계작지모가우슬탕(桂芍知母加牛膝湯) 약침이 류마티스 관절염 생쥐에 미치는 영향

정순현 ( Soon Hyun Jung ),조종관 ( Chong Kwan Cho ),김소연 ( So Yun Kim ),김영일 ( Young Il Kim ) 대전대학교 한의학연구소 2016 혜화의학회지 Vol.24 No.2

Objectives : The purpose of this study is to prove the effect and mechanism of Gamikyejakjimogawusul-tang(GKHA) herbal acupuncture on induced rheumatoid arthritis model of DBA/1 mice. Methods : We check effect of GKHA extract on the AST, ALT, Creatinine, BUN of serum and cell viability of GK extract in RAW 264.7 cells to test the stability of this study. In vitro, we measure total phenol contents, total flavonoid contents, DPPH free radical scavenging activity, ABTS cation radical scavenging activity of Gamikyejakjimogawusul-tang, effect of GK extract on ROS(Reactive Ooxygen Species) production to estimate a anti-oxidant capacity, and we also measure effect of GK extract on NO (Nitric Oxid), IL-1β, IL-6, IL-17, IL-21, TNF-α, MCP-1, GM-CSF production in RAW 264.7 cells to estimate a anti-inflammatory efficacy. In vivo, we compare a rheumatoid arthritis manifestation between control and experimental group and estimate a AI. Then we check effect of GKHA on the level of WBC, neutrophil, lympocyte, monocyte in the blood to see the effect of immune cells in blood. In addition we measure effect of GKHA on the level of hs-CRP, IgM, IgG, IL-1β, IL-6, IL-17, IL-21, TNF-α, MCP-1, GM-CSF in serum. We observe effects of GKHA on imaging of cartilage degeneration using micro CT-arthrography in paw hind. And we calculate effects of GKHA that reduced BV ratio, BS/BV ratio using 3D Micro-CT. Lastly we observe effects of GKHA histopathologic examination analysis. Results : 1. The toxicity on liver and kidney was disregardable and the cytotoxicity against RAW 264.7 cells was also disregardable. < In vitro > 1. Total phenol contents and total flavonoid contents in GK extract were in high level. 2. DPPH free radical scavenging activity and ABTS cation radical scavenging activity were increased according to concentration of GK extract 3. ROS production was significantly decreased in GK extract (at 10, 100 ㎍/ml). 4. NO, IL-6, TNF-α, MCP-1 production were significantly decreased in GK extract(at 10, 100 ㎍/ml). IL-17, GM-CSF production were significantly decreased in GK extract(at 1, 10, 100 ㎍/ml). IL-1β, IL-21 production were also decreased but there was no statistical significance. 5. 25x observation after H&E and M-T staining, infiltration of immune cells and subsidence of the cartilage and damage to the synovial cells were decreased. Conclusions : This study showed that GKHA extract had anti-oxidant capacity, anti-inflammatory efficacy. GKHA extract also had inhibiting effect on the process of rheumatoid arthritis and can protect joint and cartilage. So we expect that GKHA extract can be a meaningful treatment to rheumatoid arthritis patients.

저열관성 사출성형의 새로운 접근

尹倧淳,盧日瑢 國立 昌原大學校 産業技術硏究所 1988 産技硏論文集 Vol.2 No.-

This paper deals with a new approach to the application of the LTIM(Low Thermal Inertia Molding) Concept for molding a plastic product. Thermoelectric modules are used to form a mold cavity. By controlling the polarity of power input to the thermo-electric modules, a mold cavity surface can be rapidly heated and cooled down. An experiment was conducted on the thermoelectric modules to test the feasibility of using these modules as a mold cavity. A prototype mold was designed for the experiment and the modules were controlled by a temperature control system designed in Goldstar Co. A general injection molding machine was interfaced with the temperature control system. Some tests were conducted to compare the LTIM mold with a conventional mold.

새로운 Cyclic Oxalate의 합성

김윤영,김남령,이용균,최순규,이시우,정대일 東亞大學校 1993 東亞論叢 Vol.30 No.1

As a new protective group for diols, cyclic oxalates (20) (24) were synthesized by using oxalyl chloride, ethyl oxalyl chloride, diethyl oxalate and oxalic acid. In the reaction of diol with oxalyl chloride, the product was a mixture of the cyclic oxalate (20) and mono oxalated(21). When ethyl oxalyl chloride was used which pyridine as base, acyclic oxalates (22) (25) were obtained. Whereas ethyl oxalyl chloride which triethylamine instead of pyridine, cyclic oxalates (20) (24) as well as acyclic oxalates (22) (25) were obtained. Using diethyl oxalate, the amounts of cyclic oxalates (20) (24) were increased, but there were some difficulties in separations of its desired products. Oxalic acid, however, did not afford cyclic oxalates but gave formate which probably was formed by decarboxylation of hydroxyoxalyl group.

1-Phenylindole Ester류의 합성과 반응성 연구

崔舜圭,金兪瑛,兪丙國,李龍均,鄭大一 東亞大學校附設基礎科學硏究所 1993 基礎科學硏究論文集 Vol.10 No.1

일반적인 dienophile에 대한 pyrrole의 반응은 pyrrole이 diene system으로 작용된 [4+2] 고리부가반응과 pyrrole의 α-탄소 위치에 부가되는 Michael형태의 반응이 알려져 있다.

정보기술의 사용과 만족에 관한 통합모형 연구

유일,노춘섭,소순후,이석인,나광윤 한국정보전략학회 2000 한국정보전략학회지 Vol.3 No.1

Studies in individual level on Information Technology(IT) success are developed two areas: Studies on IT acceptance and use. Studies on end-user satisfaction. This study tried to combine two areas and develop the research model - the IT use and satisfaction model. The research model in empirically tested using data collected from end-user of groupware systems. The results of this survey are significant to general fitness of the research model. The rusults are summarized as follows: (1) perceived ease of use have significant positive influence on perceived usefulness. (2) perceived ease of use have not significant on attitude. (3) perceived usefulness have significant positive influence on attitude. (4) attitude have significant positive influence on groupware use. (5) groupware use have significant positive influence on end-user satisfaction. (6) end-user satisfaction have significant negative on groupware use.

새로운 Cyclic Oxalate의 반응성 연구

鄭大一,金侖瑛,崔舜圭,李龍均 東亞大學校 大學院 1994 大學院論文集 Vol.19 No.-

As a new protective group for diols, cyclic oxalates (6) (7) were synthesized by using oxalyl chloride, rthyl oxalyl chloride, diethyl oxalate and oxalic acid. Cyclic oxalates were readily cleaved to the corresponding diols by various bases(CH₃ONa/CH₃OH, K₂CO₃/CH₃OH, Et₃N/CH₂Cl₂1% NaOH/CH₂Cl₂, LiAlH₄/CH₂Cl₂) but were stable in acid(p-TsOH).

위 점막하 종양으로 발현된 동정맥 기형 1예

백순구,조미연,김준명,권상옥,이동기,김윤미,최윤종,김일회 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.4

Arteriovenous malformations (AVM) are an unusual cause of gastrointestinal (GI) bleeding. But most GI AVM can occur with GI bleeding. When conventional diagnostic studies have failed to reveal the source of GI bleeding, AVM have been one of the most common causes. But the incidence of AVM presented as bleeding of a gastric submucosal tumor is very low. A case of gastric submucosal tumor was experienced and reported as an AVM. This case is herein discussed with review of corresponding literature.

솔잎(갈비)·솔방울의 수처리에 관한 연구

최순규,엄기수,성현순,김윤영,이시우,정대일,이용균 동아대학교 환경문제연구소 1994 硏究報告 Vol.17 No.2

The absorption ability of low density heavy metal of pine needles and cones is better than one of existing absorption materials in treatment ability and experimental condition. The elimination ability of chlorine of pine needles and cones is better than one of active carbon. In elimination of trihalomethane, the effect of treatment of pine needles is lower than one of pine cones. And surface of pine needles and cones after absorption of heavy metal ion was observed by Scanning Electron Microscope(SEM). Application and availability of pine needles and cones as absorption material need lots of experiments. The experiment on Gal-Bi(fallen leaves) is proceeding.

당뇨병성 동결 견에서 혈관내피 세포 성장인자의 출현

김용환,권순용,김진영,박일중,조윤경,한창환 대한정형외과연구학회 2003 대한정형외과연구학회지 Vol.6 No.2

술폰기가 도핑된 폴리피를 제조 및 전기화학적 특성

박종호,김지윤,장순호,조영일 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.4

정전류 중합법으로 도펀트의 화학적 구조와 크기에 따라 폴리피롤 막을 ITO 전극위에 합성하고 전기화학적 특성을 알아보았다. 전해중합 효율은 SBA, PVS, BDS 순으로 증가하였고 합성 전기량의 증가에 따라서는 변화되지 않았다. SBA를 도펀트로 제조된 폴리피롤 막은 산화 과정에서 음이온 및 양이온 교환특성이 나타남을 CV를 통해 알 수 있었다. 도펀트가 BDS인 폴리피롤 막은 전류밀도 1 mA/㎠에서 가장 높은 속도 상수 값을 나타내었다. 또한 선형분극 법으로 측정한 확산계수는 이중층 용량 성분 때문에 임피던스 법으로 구한 값보다 크게 나타났다. According to chemical structure and size of dopant, the polypyrrole films were prepared by constant current polymerization method on ITO electrode and its electrochemical properties were investigated. The current efficiency of polymerization of pyrrole was increased in the sequence 4-sulfobenzoic acid(SBA), polyvinylsulfonic acid(PVS), 1.3-benzenedis-ulfonic acid(BDS) and was unchanged with electricity of polymerization. As a result of cyclic voltammetry of polypyrrole film prepared with SBA as dopant, ion-exchange behavior both of anion and cation was observed during oxidation process. The maximum rate constant of polypyrrole doped with BDS was obtained at 1 ㎃/㎠. In addition, the diffusion coefficient calculated by linear polarization method was more large than that measured by impedance analysis due to double layer capacitance.