Production of soybean transgenic plants to improve agronomic traits

Yoon Jeong Lee,Jin Ho Yang,Jin Sol Park,Hye Jeong Kim,Hyun Suk Cho,Jae Seong Kim,Hyun Hee Im,Ki Jung Lee,Jeong Il Kim,Soon Chun Jeong,Dong Hee Lee,Yung Soo Chung 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Soybean is a crop of importance economically and nutritionally in many parts of the world. Thanks to many new genes brought from genomic research, It is possible to introduce various candidate genes through genetic transformation to see the performance of the genes in field. In our lab, soybean transformations have been tried for last 10 years to probe the possibility of traits improvement by transformation of new gene into soybean. For this purpose, three different genes were transformed into Korean soybean variety, Kwangan. First, the gene that controls early flowering of plant was transformed into Kwangan. Second, a candidate gene for soybean mosaic virus (SMV) resistance was transformed to produce transgenic plants. Third, another candidate gene for drought tolerance was transformed. All the transgenic plants from three genes transformation were produced for their gene insertion and their expression using PCR, qRT-PCR. Further analysis including harvesting seeds is currently undertaken.

Gender and Age Difference on Driving Information Perception for Driving Context

Sol Hee Yoon,Hwan Hwangbo,Jong Kyu Choi,Yong Gu Ji,Seung Hwan Lee,Jae Heok Ryu 대한인간공학회 2011 대한인간공학회 학술대회논문집 Vol.2011 No.10

Objective: This research analyze the differences encounter on drivers' perceived importance and verification frequency on the different types of driving information with regard to gender and age. Background: Previous studies showed that there are scarcities of researches related to the clustering of in-vehicle information. Mostly focus on the development of in vehicle information system and importance motor ability for older drivers. Method: Our research concentrates on the clustering of information and analysis of perceived importance and verification frequency between age and gender. First, we realized a categorization of the information presented in automobile by: (1) Warning, (2) Driving Information, and (3) In-Vehicle Technology. Then, we proceed with a survey analysis on the information, distributed in groups depending on gender and age. Results: Results showed that there exist significant difference between groups for perceived importance and verification frequency only for warning information. However, each of the specific information showed significant difference between groups. Conclusion: Consequently, there are differences in perceived importance and verification frequency on the lower level of in-vehicle information as each group presents different requirements, purpose of use, and level of knowledge. Application: Therefore, this result can be utilized when designing an adapted automobile, taking into consideration the target gender and age of each automobile. Thus, satisfy the needs and requirement of each group.

Slide Session : OS-ALG-10 ; Allergy : Relationship of Extended Nitric Oxide Analysis with Bronchial Hyperresponsiveness (BDR) and Brconhodilator Response (BDR) in Children with Asthma According to Atopy

( Yoon Hee Kim ),( Young A Park ),( In Suk Sol ),( Seo Hee Yoon ),( Min Jung Kim ),( Kyung Won Kim ),( Myung Hyun Sohn ),( Kyu Earn Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Extended nitric oxide (NO) analysis such as alveolar NO has been studied ardently because it is not affected by exhaled flow rate. The aim of this study is to evaluate whether extended nitric oxide analysis parameters are correlated with bronchial hyperresponsiveness (BHR) and Brconhodilator response (BDR). Methods: Children with a typical asthmatic symptom were evaluated. The exhaled nitric oxide test was done with methacholine challenge test for BHR for the children and spirometry before and after bronchodilator for BDR for the other children. We measured exhaled nitric oxide at 30, 50, 100, 200 mL/s and calculated JawNO (total NO flux). Alveolar NO, NO transfer factor and airway wall NO (CawNO) using Hogman and Merilainen algorithm. Atopy was defined as a positive skin test result or more than 0.7 KUa/L specific lgE or a more than 150 IU/ml total lgE. Results: In atopic children, JawNO was significantly higher in the positive BHR (PC20 < 16 mg/ml) than in the negative BHR subjects and correlated negatively with PC20. In atopic children, JawNO was significantly higher in the positive BDR (ΔFEV1 = 12%) than in the negative BDR subjects and correlated positively with ΔFEV1. In non-atopic children, CawNO was significantly higher in the positive BHR than in the negative BHR subjects and correlated negatively with PC20. In non-atopic children, CawNO was significantly higher in the positive BDR than in the negative BDR subjects and correlated positively with ΔFEV1. Conclusions: The relationship of extended nitric oxide analysis parameters with BHR and BDR was different according to atopy. The JawNO in the atopy and the CawNO in the non-atopy might give useful information for understanding BHR and BDR in children with asthma.

A Study on the Effect of Non-Driving Related Task in Take-over task for Highly Automated Driving

Sol Hee Yoon(윤솔희) 대한인간공학회 2021 대한인간공학회 학술대회논문집 Vol.2021 No.11

Objective: The aim of this study is to investigate the effect of non-driving related task attributes on drivers’ takeover task in highly automated driving. Background: The introduction of new assistive technologies improves safe driving and reduce car accidents while changes the drivers’ behavior. These changes highly automated driving concerns with the importance of drivers being out of the loop and engaging in task not related to driving. Thus, highly automated driving have to take consideration about issues related to the transition of control between the vehicle and the drivers. Method: A driving simulator experiment was conducted to analyze the effect of non-driving related task attributes on the process of transition of control in highly automated driving by analyzing and investigating the takeover performance and quality of drivers. Tasks attributes were categorized depending on the visual, physical, and cognitive demand of the non-driving related task. The measures used to evaluate the transition of control were categorized into visual (gaze on time, fixation time) performance, physical (hand on time) and overall takeover time. Results: Results from the experiment showed a significant effect on non-driving task attributes on takeover performance and quality. In addition, the current study presented a conceptual model to explain the transition of control in highly automated driving based on non-driving related task attributes engaged during automated driving. Conclusion: The results from this study provides insights and understanding on the process of transition of control between the drivers and the automated vehicle system taking into consideration the drivers’ state before the takeover request and the drivers’ performance after the takeover has been conducted.

Serum clusterin level in children with atopic dermatitis

Sol, In Suk,Kim, Yoon Hee,Lee, Kyung Eun,Hong, Jung Yeon,Kim, Mi Na,Kim, Yun Seon,Oh, Mi Seon,Kim, Min Jung,Yoon, Seo Hee,Park, Young A.,Kim, Kyung Won,Sohn, Myung Hyun,Kim, Kyu-Earn Oceanside Publications 2016 Allergy and asthma proceedings Vol.37 No.4

소아에서 혈청 25-hydroxyvitamin D와 알레르기비염의 연관성

윤서희 ( Seo Hee Yoon ),김정윤 ( Jung Yoon Kim ),김윤희 ( Yoon Hee Kim ),박영아 ( Young A Park ),설인숙 ( In Suk Sol ),김민정 ( Min Jung Kim ),김경원 ( Kyung Won Kim ),손명현 ( Myung Hyun Sohn ),김규언 ( Kyu-earn Kim ) 대한천식알레르기학회(구 대한알레르기학회) 2016 Allergy Asthma & Respiratory Disease Vol.4 No.6

Purpose: Vitamin D is known to play an important role in the regulation of the immune system and in the prevention of allergic disease. Herein, we examined the association between vitamin D and allergic rhinitis (AR) in children. Methods: A total of 72 aeroallergen-proven AR and 57 healthy control subjects who visited Severance Children`s Hospital between 2012 and 2014 were evaluated. The mean serum 25-hydroxyvitamin D (25(OH)D) level was measured in all subjects to evaluate their vitamin D status. Results: The mean serum 25(OH)D levels was significantly lower in patients with AR than in controls (19.5±6.4 ng/mL vs. 22.3±5.8 ng/mL, P=0.013). The incidence of AR is higher in the 25(OH)D-insufficient group (15-20 ng/mL) and in the 25(OH)D-deficient group (≤15 ng/mL) compared with the 25(OH)D-sufficient group (>20 ng/mL) (40.3% vs. 84.4% vs. 22.2%, P<0.001). There was no significant difference in the serum 25(OH)D level between subgroups with mild and moderate-to-severe AR symptoms (19.8±6.5 vs. 19.0±6.3, P=0.596). After adjusting for confounding factors, the lower serum vitamin D level groups had increased odds of AR compared with the 25(OH)D-sufficient group (odds ration [95% confidence interval]; 3.67 [1.29-10.38] in the 25(OH)D-insufficient group; P=0.014 and 6.04 [1.49-24.51] in the 25(OH)D-deficient group; P=0.012). Conclusion: The serum vitamin D levels were significantly lower in children with AR compared with healthy controls. Therefore, lower serum level of 25(OH)D may be associated with the development of AR. (Allergy Asthma Respir Dis 2016:4:423-428)

소아 천식 환자에서 전신 염증 유형에 따른 천식의 특성

설인숙 ( In Suk Sol ),김윤희 ( Yoon Hee Kim ),이희선 ( Hee Seon Lee ),김민정 ( Min Jung Kim ),한윤기 ( Yoon Ki Han ),박영아 ( Young A Park ),김경원 ( Kyung Won Kim ),손명현 ( Myung Hyun Sohn ),김규언 ( Kyu Earn Kim ) 대한소아알레르기호흡기학회 1992 소아알레르기 및 호흡기학회지 Vol.2 No.3

Purpose: Asthma is a chronic airway inflammation. We evaluated whether systemic inflammatory patterns could reflect the nature of airway inflammation. We assessed characteristics of asthma according to systemic inflammatory patterns. Methods: A total of 413 children with asthma were enrolled in the study. Four systemic inflammatory patterns were classified according to eosinophil and neutrophil counts in peripheral blood. Children with neutrophil count≥5,000/μL were defined as the NEUhi group, those with neutrophil count <2,720/μL as the NEUlo group. The intermediate group with neutrophil count between 2,720/μL and 5,000/uL was excluded from the study. Children with eosinophil≥650/μL were defined as the EOShi group, those with eosinophil count<240/μL as the EOSlo group. The remaining patients were excluded from the study. The characteristics of asthma include pulmonary function test results, bronchodilator response, airway hyperresponsiveness, and atopy. Results: The EOShi group had a lower PC20 (provocative concentration of methacholine causing a 20% fall in forced expiratory volume in 1 second [FEV1]), a lower FEV1, and a higher immunoglobulin E level rather than the EOSlo groups, although there were no significant differences between the NEUhi and NEUlo groups. The eosinophil percentages of the induced sputum samples were higher in the EOShi group than the EOSlo group and correlated with blood eosinophil counts. Conclusion: Eosinophilic inflammation was related to characteristics of asthma and sputum eosinophils. However, neutrophilic in- flammation reflected neither asthma features, sputum neutrophils, nor eosinophilic inflammation. Further studies on blood neutrophils involving asthma phenotypes in terms of more specific characteristics of asthma should be needed in children. (Allergy Asthma Respir Dis 2014;2:165-170)

가와사키병 환아에서 아스피린 치료 후 나타난 호산구증가증 1예

설인숙 ( N Suk Sol ),최명현 ( Myung Hyun Choi ),김민정 ( Min Jung Kim ),김윤희 ( Yoon Hee Kim ),이희선 ( Hee Seon Lee ),한윤기 ( Yoon Ki Han ),김기환 ( Ki Hwan Kim ),김경원 ( Kyung Won Kim ),손명현 ( Myung Hyun Sohn ),김규언 ( Kyu 대한천식알레르기학회(구 대한알레르기학회) 2014 Allergy Asthma & Respiratory Disease Vol.2 No.2

Drug hypersensitivity is one of drug adverse reactions that develop in susceptible patients following exposure to certain drugs and cannot be predicted from the known pharmacology of a drug. Severe hypersensitivity is associated with high morbidity and mortality. Although the issue of hypersensitivity to nonsteroidal anti-inflammatory drugs (NSAIDs) has been largely investigated in adults, data related to NSAIDs hypersensitivity is insufficient in childhood. And in spite of the recommendation to avoid use of aspirin due to Reye syndrome in children, aspirin is one of major treatment along with intravenous immunoglobulin in Kawasaki disease. We report a case of a 10-month-old boy who underwent intravenous immunoglobulin and aspirin treatment for Kawasaki disease, and subsequently revealed severe leukocytosis and eosinophilia. To our knowledge, there have been no previous reportsof aspirin-induced eosinophilia in Korea. (Allergy Asthma Respir Dis 2014;2:142-145)

소아 천식 환자에서 전신 염증 유형에 따른 천식의 특성

설인숙 ( In Suk Sol ),김윤희 ( Yoon Hee Kim ),이희선 ( Hee Seon Lee ),김민정 ( Min Jung Kim ),한윤기 ( Yoon Ki Han ),박영아 ( Young A Park ),김경원 ( Kyung Won Kim ),손명현 ( Myung Hyun Sohn ),김규언 ( Kyu Earn Kim ) 대한천식알레르기학회 2014 Allergy Asthma & Respiratory Disease Vol.2 No.3

Purpose: Asthma is a chronic airway inflammation. We evaluated whether systemic inflammatory patterns could reflect the nature of airway inflammation. We assessed characteristics of asthma according to systemic inflammatory patterns. Methods: A total of 413 children with asthma were enrolled in the study. Four systemic inflammatory patterns were classified according to eosinophil and neutrophil counts in peripheral blood. Children with neutrophil count≥5,000/μL were defined as the NEUhi group, those with neutrophil count <2,720/μL as the NEUlo group. The intermediate group with neutrophil count between 2,720/μL and 5,000/uL was excluded from the study. Children with eosinophil≥650/μL were defined as the EOShi group, those with eosinophil count<240/μL as the EOSlo group. The remaining patients were excluded from the study. The characteristics of asthma include pulmonary function test results, bronchodilator response, airway hyperresponsiveness, and atopy. Results: The EOShi group had a lower PC20 (provocative concentration of methacholine causing a 20% fall in forced expiratory volume in 1 second [FEV1]), a lower FEV1, and a higher immunoglobulin E level rather than the EOSlo groups, although there were no significant differences between the NEUhi and NEUlo groups. The eosinophil percentages of the induced sputum samples were higher in the EOShi group than the EOSlo group and correlated with blood eosinophil counts. Conclusion: Eosinophilic inflammation was related to characteristics of asthma and sputum eosinophils. However, neutrophilic inflammation reflected neither asthma features, sputum neutrophils, nor eosinophilic inflammation. Further studies on blood neutrophils involving asthma phenotypes in terms of more specific characteristics of asthma should be needed in children.(Respir Dis 2014;2:165-170)

Etc. ; The Korean version of the FRAIL scale: clinical feasibility and validity of assessing the frailty status of Korean elderly

( Hee Won Jung ),( Hyun Jung Yoo ),( Si Young Park ),( Sun Wook Kim ),( Jung Yeon Choi ),( Sol Ji Yoon ),( Cheol Ho Kim ),( Kwang Il Kim ) 대한내과학회 2016 The Korean Journal of Internal Medicine Vol.31 No.3

Background/Aims: The fatigue, resistance, ambulation, illnesses, and loss of weight (FRAIL) scale is a screening tool for frailty status using a simple 5-item questionnaire. The aim of this study was to evaluate the clinical feasibility and validity of the Korean version of the FRAIL (K-FRAIL) scale. Methods: Questionnaire items were translated and administered to 103 patients aged ≥ 65 years who underwent a comprehensive geriatric assessment at the Seoul National University Bundang Hospital. In this cross-sectional study, the K-FRAIL scale was compared with the domains and the multidimensional frailty index of the comprehensive geriatric assessment. We also assessed the time required to complete the scale. Results: The participants’ mean age was 76.8 years (standard deviation [SD], 6.1), and 55 (53.4%) were males. The mean overall frailty index was 0.19 (SD, 0.17). For K-FRAIL-robust, prefrail, and frail patients, the mean frailty indices were 0.09, 0.18, and 0.34, respectively (p for trend < 0.001). A higher degree of impairment in the K-FRAIL scale was associated with worse nutritional status, poor physical performance, functional dependence, and polypharmacy. The number of items with impairment in the K-FRAIL scale was positively associated with the frailty index (B = 3.73, p < 0.001). The K-FRAIL scale could differentiate vulnerability from robustness with a sensitivity of 0.90 and a specificity of 0.33. Of all patients, 75 (72.8%) completed the K-FRAIL scale within < 3 minutes. Conclusions: The K-FRAIL scale is correlated with the frailty index and is a simple tool to screen for frailty in a clinical setting.