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Test and Simulation for the Response of PLS-II Button Type Beam Position Monitor
Lee, Sojeong,Hong, Juho,Ko, In Soo,Kim, Changbum,Kim, Do Tae,Park, Hong Jib,Lee, Eun Hee,Lee, Jin Won,Kim, Seunhwan,Park, Yong-jung,Park, Sung-Ju Korean Physical Society 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.59 No.2
글루탐산 생산을 위한 Lactococcus lactis HY7803 균주의 대두 발효 적용
Lee Jungmin,Heo Sojeong,Choi Jihoon,Pyo Eunji,Lee Myounghee,Shin Sangick,Lee Jaehwan,Lee Junglyoul,정도원 한국미생물·생명공학회 2023 한국미생물·생명공학회지 Vol.51 No.1
The glutamic acid producing strain for development savory taste enhancing foods was identified the possibility through application into soybean fermentation. To check the effects on glutamic acid production during soybean fermentation, Lactococcus lactis HY7803 was introduced as a starter. The soybean samples were analyzed on days 0, 7, 14 and 21. The numbers of bacteria decreased gradually, while the content of amino-type nitrogen increased during fermentation in the soybean with L. lactis HY7803. Glutamic acid content in soybeans with L. lactis HY7803 increased from 114.99 ± 9.37 pmol/ul on day 0 to 138.14 ± 1.76 pmol/ul on day 21, showing an overall higher amino acid content than soybeans without L. lactis HY7803 and similar content to soybeans with Aspergillus oryzae SNU-G. It was clearly distinguished through principal component analysis. Consequently, our results indicate that L. lactis HY7803 is available as a fungus replacement and may be a good starter strain for enhancing savory taste in vitro as well as soybean fermentation.
Yun Sojeong,Ryu Ji Hyeong,Jang Joo Hee,Bae Hyunjoo,Yoo Seung-Hyo,Choi Ae-Ran,Jo Sung Jin,Lim Jihyang,Lee Jehoon,Ryu Hyejin,Cho Sung-Yeon,Lee Dong-Gun,Lee Jongmin,Kim Seok Chan,Park Yeon-Joon,Lee Hyeyo 대한진단검사의학회 2021 Annals of Laboratory Medicine Vol.41 No.6
Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody assays have high clinical utility in managing the pandemic. We compared antibody responses and seroconversion of coronavirus disease 2019 (COVID-19) patients using different immunoassays. Methods: We evaluated 12 commercial immunoassays, including three automated chemiluminescent immunoassays (Abbott, Roche, and Siemens), three enzyme immunoassays (Bio-Rad, Euroimmun, and Vircell), five lateral flow immunoassays (Boditech Med, SD biosensor, PCL, Sugentech, and Rapigen), and one surrogate neutralizing antibody assay (GenScript) in sequential samples from 49 COVID-19 patients and 10 seroconversion panels. Results: The positive percent agreement (PPA) of assays for a COVID-19 diagnosis ranged from 84.0% to 98.5% for all samples (>14 days after symptom onset), with IgM or IgA assays showing higher PPAs. Seroconversion responses varied across the assay type and disease severity. Assays targeting the spike or receptor-binding domain protein showed a tendency for early seroconversion detection and higher index values in patients with severe disease. Index values from SARS-CoV-2 binding antibody assays (three automated assays, one LFIA, and three EIAs) showed moderate to strong correlations with the neutralizing antibody percentage (r=0.517–0.874), and stronger correlations in patients with severe disease and in assays targeting spike protein. Agreement among the 12 assays was good (74.3%–96.4%) for detecting IgG or total antibodies. Conclusions: Positivity rates and seroconversion of SARS-CoV-2 antibodies vary depending on the assay kits, disease severity, and antigen target. This study contributes to a better understanding of antibody response in symptomatic COVID-19 patients using currently available assays.
( Sojeong Heo ),( Jungmin Lee ),( Jong-hoon Lee ),( Do-won Jeong ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.10
To shed light on the genetic basis of salt tolerance in Enterococcus faecium, Enterococcus faecalis, and Tetragenococcus halophilus, we performed comparative genome analysis of 10 E. faecalis, 11 E. faecium, and three T. halophilus strains. Factors involved in salt tolerance that could be used to distinguish the species were identified. Overall, T. halophilus contained a greater number of potassium transport and osmoprotectant synthesis genes compared with the other two species. In particular, our findings suggested that T. halophilus may be the only one among the three species capable of synthesizing glycine betaine from choline, cardiolipin from glycerol and proline from citrate. These molecules are well-known osmoprotectants; thus, we propose that these genes confer the salt tolerance of T. halophilus.
Impact of Device Area and Film Thickness on Performance of Sol-Gel Processed ZrO<sub>2</sub> RRAM
Lee, Sojeong,Kim, Taegyun,Jang, Bongho,Lee, Won-yong,Song, Kyu Chan,Kim, Hyun Seo,Do, Gyung Yeop,Hwang, Seung Bum,Chung, Seungjun,Jang, Jaewon IEEE 2018 IEEE electron device letters Vol.39 No.5
<P>A sol-gel-processed ZrO<SUB>2</SUB> layer was used as an active layer for a resistive random-access memory (RRAM). With top Ag electrodes, the fabricated devices show conventional bipolar switching memory properties. The impacts of device size and ZrO<SUB>2</SUB> film thickness on device performance were investigated. The scaling of the device area and the successful increase in ZrO<SUB>2</SUB> film thickness increased the high-resistance state (HRS)/low-resistance state (LRS) ratio values and improved the non-volatile memory properties, such as retention and endurance. The fabricated ITO/ZrO<SUB>2</SUB>/Ag RRAM shows good retention and endurance properties. The HRS and LRS were found to last for 10<SUP>4</SUP> s without any significant degradation.</P>
Diagnostic imaging findings of subcutaneous mast cell tumor in a dog
Sojeong Kwon,Min Jang,Sang-Kwon Lee,Kija Lee 대한수의학회 2023 大韓獸醫學會誌 Vol.63 No.2
A 5-year-old Labrador Retriever presented with a subcutaneous mass on the right scapular region with localized calor. On magnetic resonance imaging (MRI), a heterogeneously hypointense mass was seen on T1-weighted images (WI) and hyperintense on T2WI and fat-suppressed T2WI with marginal contrast enhancement on postcontrast T1WI and invasion into the surrounding tissue. Histopathological examination of the mass confirmed subcutaneous mast cell tumor (MCT) infiltrating to the adjacent subcutaneous fat. This is the first report to describe the MRI findings of subcutaneous MCT in veterinary medicine. This study suggests that MRI may help evaluate the extent of invasion of subcutaneous MCT.
DNA binding fluorescent proteins for the direct visualization of large DNA molecules
Lee, Seonghyun,Oh, Yeeun,Lee, Jungyoon,Choe, Sojeong,Lim, Sangyong,Lee, Hyun Soo,Jo, Kyubong,Schwartz, David C. Oxford University Press 2016 Nucleic acids research Vol.44 No.1
<P>Fluorescent proteins that also bind DNA molecules are useful reagents for a broad range of biological applications because they can be optically localized and tracked within cells, or provide versatile labels for <I>in vitro</I> experiments. We report a novel design for a fluorescent, DNA-binding protein (FP-DBP) that completely ‘paints’ entire DNA molecules, whereby sequence-independent DNA binding is accomplished by linking a fluorescent protein to two small peptides (KWKWKKA) using lysine for binding to the DNA phosphates, and tryptophan for intercalating between DNA bases. Importantly, this ubiquitous binding motif enables fluorescent proteins (<I>K</I><SUB>d</SUB> = 14.7 μM) to confluently stain DNA molecules and such binding is reversible via pH shifts. These proteins offer useful robust advantages for single DNA molecule studies: lack of fluorophore mediated photocleavage and staining that does not perturb polymer contour lengths. Accordingly, we demonstrate confluent staining of naked DNA molecules presented within microfluidic devices, or localized within live bacterial cells.</P>