인체 대장암 세포주 SW480에서 docosahexaenoic acid에 의한 침윤억제 기전

Soyeon Shin(신소연),Yong-Jo Kim(김용조),Kyoung-Sub Song(송경섭),Kaipeng Jing,Nayeong Kim(김나영),Soyeon Jeong(정소연),Ji-Hoon Park(박지훈),Kang-Sik Seo(서강식),Jun-Young Heo(허준영),Hyun-Joo Kwon(권현주),Jong-Il Park(박종일),Seung 한국생명과학회 2010 생명과학회지 Vol.20 No.4

대장암은 미국 등 서양 국가뿐만 아니라 국내에서도 2번째로 많이 발병이 되는 암으로 알려져 있다. 역학조사에 의하면 ω3-PUFAs를 많이 섭취한 인종에서 대장암 발생빈도가 감소하고 최근 ω3-PUFAs는 수종의 암에 대해 항암작용을 나타낸다고 한다. 이에 본 연구에서는 대장암에서 DHA 등 ω3-PUFA의 항침윤 기전을 규명하여 다음과 같은 결과를 얻었다. DHA 및 EPA는 대장암 세포주 SW480의 증식을 농도 의존적으로 억제하였으나 AA는 거의 영향이 없었으며 TUNEL assay로 apoptotic cell death가 확인 되었다. DHA는 β-catenin 단백 및 TCF/LEF luciferase 활성을 농도 의존적으로 억제 하였다. SW480 세포의 침윤능은 DHA의 농도에 의존적으로 억제되었다. DHA처리 후 MMP-9 및 MMP-2 mRNA양이 감소되었을 뿐만 아니라 그 promoter의 reporter 활성도 억제되었다. NF-kB 및 p-IkB 단백짙양도 DHA의 처리농도에 의존적으로 감소하였으며 NF-kB promoter의 활성도 억제되었다. 이상의 결과로 ω3-PUFA는 대장암에서 NF-kB 신호전달 차단에 의한 MMP-2 및 MMP-9 발현을 억제하여 침윤을 억제하여 항암작용을 나타낼 수 있음을 시사하며, 따라서 ω3-PUFA는 대장암의 예방 및 치료에 유용하게 사용될 수 있으리라 생각된다. Colon cancer is one of the most common malignancies in the western world and the second leading cause of cancer death in Korea. Epidemiology studies have shown a reduced incidence of colon cancer among populations consuming a large quantity of ω3-polyunsaturated fatty acids (ω3-PUFA) of marine origin. Recently, it has been found that ω3-PUFA has an antineoplastic effect in several cancers. This study was designed to investigate the mechanism of the anti-invasive effect of ω3-PUFA in colon cancer. ω3-PUFA, docosahexaenoic acids (DHA) and eicosapentaenoic acid (EPA) treatment resulted in a dose-dependent inhibition of cell growth in SW480 human colon cancer cells. In contrast, arachidonic acid (AA), a ω6-PUFA, exhibited no significant effect. This action likely involves apoptosis, given that DHA treatment increased apoptotic cells in TUNEL assay. Moreover, invasiveness of SW480 cells was inhibited following treatment of DHA in a dose-dependent manner; in contrast, AA had no effect. The levels of MMP-9 and MMP-2 mRNA decreased after DHA pretreatment. MMP-9 and MMP-2 promoter activities were also inhibited by DHA treatment. The levels of NF-kB and p-IkB protein were down-regulated by DHA pretreatment in a dose dependent manner. In addition, DHA inhibited NF-kB promoter reporter activities. These findings suggest that ω3-PUFA may inhibit cancer cell invasion by inhibition of MMPs via reduction of NF-kB in colon cancer. In conclusion, ω3-PUFA could be used for chemoprevention and treatment of human colon cancer.

Use of a Comprehensive Geriatric Assessment to Predict Short-Term Postoperative Outcome in Elderly Patients With Colorectal Cancer

Lee, Yoon Hyun,Oh, Heung-Kwon,Kim, Duck-Woo,Ihn, Myong Hoon,Kim, Jee Hyun,Son, Il Tae,Kang, Sung Il,Kim, Gwang Il,Ahn, Soyeon,Kang, Sung-Bum The Korean Society of Coloproctology 2016 Annals of Coloproctolgy Vol.32 No.5

<P><B>Purpose</B></P><P>This study was conducted to identify the effectiveness of a preoperative comprehensive geriatric assessment (CGA) for predicting postoperative morbidity in elderly patients who underwent surgery for colorectal cancer.</P><P><B>Methods</B></P><P>Elderly patients (≥70 years old) who underwent surgery for colorectal cancer at a tertiary hospital in Korea were identified, and their cases were analyzed using data from a prospectively collected database to establish an association between major postsurgical complications and 'high-risk' patient as defined by the CGA.</P><P><B>Results</B></P><P>A total of 240 patients, with a mean age of 76.7 ± 5.2 years, were enrolled. Ninety-five patients (39.6%) were classified as 'high-risk' and 99 patients (41.3%) as having postoperative complications. The univariate analysis indicated that risk factors for postoperative complications were age, American Society of Anesthesiologists physical status classification, serum hemoglobin, carcinoembryonic antigen, cancer stage, and 'high-risk' status. The multivariable analyses indicated that 'high-risk' status (odds ratio, 2.107; 95% confidence interval, 1.168–3.804; P = 0.013) and elevated preoperative carcinoembryonic antigen (odds ratio, 2.561; 95% confidence interval, 1.346–4.871, P = 0.004) were independently associated with postoperative complications. A multivariable analysis of the individual CGA domains indicated that high comorbidities and low activities of daily living were significantly related with postoperative complications.</P><P><B>Conclusion</B></P><P>A preoperative CGA indicating 'high-risk' was associated with major postoperative complications in elderly patients who underwent surgery for colorectal cancer. Thus, using the CGA to identify elderly colorectal-cancer patients who should be given more care during postoperative management may be clinically beneficial.</P>

First Investigation of Microbial Community Composition in the Bridge (Gadeok Channel) between the Jinhae-Masan Bay and the South Sea of Korea

Lee, Jiyoung,Lim, Jae-Hyun,Park, Junhyung,Youn, Seok-Hyun,Oh, Hyun-Ju,Kim, Ju-Hyoung,Kim, Myung Kyum,Cho, Hyeyoun,Yoon, Joo-Eun,Kim, Soyeon,Markkandan, Kesavan,Park, Ki-Tae,Kim, Il-Nam Korean Ocean Research & Development Institute and 2018 OCEAN SCIENCE JOURNAL Vol.53 No.2

Microbial community composition varies based on seasonal dynamics (summer: strongly stratified water column; autumn: weakly stratified water column; winter: vertically homogeneous water column) and vertical distributions (surface, middle, and bottom depths) in the Gadeok Channel, which is the primary passage to exchange waters and materials between the Jinhae-Masan Bay and the South Sea waters. The microbial community composition was analyzed from June to December 2016 using 16S rRNA gene sequencing. The community was dominated by the phyla Proteobacteria (45%), Bacteroidetes (18%), Cyanobacteria (15%), Verrucomicrobia (6%), and Actinobacteria (6%). Alphaproteobacteria (29%) was the most abundant microbial class, followed by Flavobacteria (15%) and Gammaproteobacteria (15%) in all samples. The composition of the microbial communities was found to vary vertically and seasonally. The orders Flavobacteriales and Stramenopiles showed opposing seasonal patterns; Flavobacteriales was more abundant in August and December while Stramenopiles showed high abundance in June and October at all depths. The genus Synechococcus reached extremely high abundance (14%) in the June surface water column, but was much less abundant in December water columns. Clustering analysis showed that there was a difference in the microbial community composition pattern between the strongly stratified season and well-mixed season. These results indicate that the seasonal dynamics of physicochemical and hydrologic conditions throughout the water column are important parameters in shaping the microbial community composition in the Gadeok Channel.

Comparative analysis between combination and single-agent chemotherapy for elderly patients with advanced non-small cell lung cancer: A nationwide population-based outcome study

Lee, Yun-Gyoo,Lee, Ju Hyun,Kim, Se-Hyun,Kim, Yu Jung,Lee, Heeyoung,Ahn, Soyeon,Jang, Joung-Soon,Lee, Jong-Seok,Kim, Jee Hyun Elsevier 2018 Lung cancer Vol.122 No.-

<P><B>Abstract</B></P> <P><B>Introduction</B></P> <P>To determine whether an upfront combination compared to single-agent therapy is beneficial for elderly patients with advanced non-small cell lung cancer (NSCLC) in the real world, a population-based epidemiologic study was conducted.</P> <P><B>Methods</B></P> <P>Patients ≥70 years with advanced NSCLC from 2007 to 2012 were identified in the National Health Insurance Service Database of Korea. A Cox proportional-hazards regression model and propensity score analysis were used to examine the effect of treatment modality on survival.</P> <P><B>Results</B></P> <P>Among 41,276 patients newly diagnosed with lung cancer, 8274 (20.0%) identified to be treated with upfront palliative chemotherapy were eligible for this study. After excluding 976 patients who received a first-line anti-epidermal growth factor receptor (EGFR) treatment, 7298 (88.2%) who received cytotoxic chemotherapy were included in further analyses: 5636 (77.2%) received doublet chemotherapy and 1662 (22.8%) received monotherapy. The most frequent regimen in combination group was gemcitabine and platinum doublet (44.7%), whereas that in monotherapy group was gemcitabine (46.7%). Multivariate analyses indicated lower use of combination chemotherapy with increasing age (odds ratio [OR] 0.73; 95% CI 0.67–0.79; <I>P</I> < 0.001) and female sex (OR 0.71; 95% CI 0.62–0.80; <I>P</I> < 0.001). Receipt of combination over single-agent chemotherapy was associated with a reduced risk of death (hazard ratio [HR] 0.91; 95% CI 0.86–0.96; <I>P</I> = 0.001) in overall population and (HR 0.89; 95% CI 0.80–0.98; <I>P</I> = 0.019) in the propensity-matched cohort.</P> <P><B>Conclusion</B></P> <P>In elderly patients with advanced NSCLC excluding those receiving frontline anti-EGFR targeted agents, receiving initial combination chemotherapy compared to single-agent was associated with improved survival.</P> <P><B>Highlights</B></P> <P> <UL> <LI> This population-based study included all patients ≥70 years of advanced NSCLC. </LI> <LI> We examined if an upfront combination compared to singlet therapy is beneficial. </LI> <LI> Combination over singlet chemotherapy was associated with a reduced risk of death. </LI> <LI> Our results were consistent between overall and propensity score-matched cohort. </LI> </UL> </P>

Categorized wetland preference and life forms of the vascular plants in the Korean Peninsula

Choung, Yeonsook,Min, Byeong Mee,Lee, Kyu Song,Cho, Kang-Hyun,Joo, Kwang Yeong,Hyun, Jin-Oh,Na, Hye Ryun,Oh, Hyun Kyung,Nam, Gi-Heum,Kim, Jin-Seok,Cho, Soyeon,Lee, Jongsung,Jung, Sangyeop,Lee, Jaeyeon The Ecological Society of Korea 2021 Journal of Ecology and Environment Vol.45 No.1

Background: In 2020, a categorized list of wetland preferences, major habitats, and life forms of 4145 vascular plant taxa occurring in the Korean Peninsula was published by the National Institute of Biological Resources. We analyzed the list and explored the distribution patterns of the five categorized groups according to wetland preference, along with the information on the major habitats and the life forms of the plants belonging to those categories. Results: Out of 4145 taxa, we found that 729 wetland plant taxa (18%) occur in Korea: 401 obligate wetland plants and 328 facultative wetland plants. Among the 729 wetland taxa, the majority (73%) was hygrophytes and the remaining 27% was aquatic macrophytes. Furthermore, almost all of the wetland taxa are herbs; so, woody plants are only 4.7%. The 16 carnivorous taxa distributed in Korea were characterized as obligate wetland plants. Conclusions: We expect the categorized information would promote understanding of the characteristics of the plant species and would be an important source for understanding, conservation, and restoration of wetland ecosystems.

Draft genomes of two morphologically similar mushrooms endemic in Korea; Ganoderma lingzhi and Perenniporia fraxinea

Hyun Jo Koo,Eun-Kyung Kim,Hwa-Yong An,Soyeon Kang,Gyoungju Nah 한국육종학회 2023 한국육종학회 공동학술발표집 Vol.2023 No.-

A population-based observational study of patients with pulmonary disorders in intensive care unit

Hyun Woo Lee,Eunjeong Ji,Soyeon Ahn,Hye-Joo Yang,Seo-Young Yoon,Tae Yeon Park,Yeon Joo Lee,Jinwoo Lee,Sang Min Lee,Seung-Hye Choi,Young-Jae Cho 대한내과학회 2020 The Korean Journal of Internal Medicine Vol.35 No.6

Background/Aims: Only a few epidemiologic studies on the patients with pulmonary disorders admitted to intensive care unit exist. We investigated the characteristics and clinical outcomes of the patients with severe pulmonary disorders. Methods: The sample cohort database of National Health Insurance Sharing Service from 2006 to 2015 was used. Operational definition of critically ill patients was adults who were either admitted to intensive care unit for at least 3 days or expired within first 2 days in the unit. The pulmonary disorder group comprised of critically ill patients with respiratory disease as the main diagnosis. Results: Among the 997,173 patients, 12,983 (1.3%) in 383 intensive care units were categorized as critically ill. Patients in the pulmonary disorder group tended to have more comorbidities or disabilities. The length of hospital stay and duration of mechanical ventilation were longer in the pulmonary disorder group. Overall mortality and re-admission were higher in the pulmonary disorder group, with adjusted incidence rate ratios of 1.22 (95% confidence interval, 1.18 to 1.27) and 1.26 (95% confidence interval, 1.17 to 1.36), respectively. After adjustment by Cox regression, the pulmonary disorder group was an independent risk factor for in-hospital mortality. Conclusions: In critically ill patients with pulmonary disorder, the use of healthcare resources was higher, and their clinical outcomes were significantly worse than the non-pulmonary disorder group.

Agastache rugosa ethanol extract suppresses bone loss via induction of osteoblast differentiation with alteration of gut microbiota

Soyeon Hong,Kwang Hyun Cha,Do Yeon Kwon,Yang Ju Son,Sang Min Kim,Jung-Hye Choi,Gyhye Yoo,Chu Won Nho 한국식품영양과학회 2020 한국식품영양과학회 학술대회발표집 Vol.2020 No.10

The Src/PLC/PKC/MEK/ERK Signaling Pathway Is Involved in Aortic Smooth Muscle Cell Proliferation induced by Glycated LDL

Hyun-Mi Cho,김태웅,최성희,Ki-Chul Hwang,Sue-Young Oh,김호경,Deok-Hyo Yoon,Myung-Ae Choi,SoYeon Lim,송희상,Yangsoo Jang 한국분자세포생물학회 2005 Molecules and cells Vol.19 No.1

Low density lipoproteins (LDL) play important roles in the pathogenesis of atherosclerosis. Diabetes is asso-ciated with accelerated atherosclerosis leading to car-diovascular disease in diabetic patients. Although LDL stimulates the proliferation of arterial smooth muscle cells (SMC), the mechanisms are not fully understood. We examined the effects of native LDL and glycated LDL on the extracellular signal-regulated kinase (ERK) pathway. Addition of native and glycated LDL to rat aorta SMCs (RASMCs) stimulated ERK phosphoryla-tion. ERK phosphorylation was not affected by expo-sure to the Ca2+ chelator BAPTA-AM but inhibition of protein kinase C (PKC) with GF109203X, inhibition of Src kinase with PP1 (5 M) and inhibition of phos-pholipase C (PLC) with U73122/U73343 (5 M) all reduced ERK phosphorylation in response to glycated LDL. In addition, pretreatment of the RASMCs with a cell-permeable mitogen-activated protein kinase kinase (MEK) inhibitor (PD98059, 5 M) markedly decreased ERK phosphorylation in response to native and gly-cated LDL. These findings indicate that ERK phos-phorylation in response to glycated LDL involves the activation of PKC, PLC, and MEK, but is independent of intracellular Ca2+.

Short Communication: Complete genome sequence of functional probiotic candidate Lactobacillus amylovorus CACC736

Soyeon Park,Jung-Ae Kim,Hyun Jun Jang,Dae-Hyuk Kim,Yangseon Kim 한국축산학회 2023 한국축산학회지 Vol.65 No.2

Lactobacillus amylovorus CACC736 was originated from swine feces in Korea. The complete genome sequences of the strain contained one circular chromosome (2,057,809 base pair [bp]) with 38.2% guanine-cytosine (GC) content and two circular plasmids, namely, pCACC736-1 and pCACC736-2. The predicted protein-coding genes, which are encoding the clustered regularly interspaced short palindromic repeats (CRISPR)-associated proteins, biosynthesis of bacteriocin (helveticin J), and the related proteins of the bile, acid tolerance. Notably, the genes related to vitamin B-group biosynthesis (riboflavin and cobalamin) were also found in L. amylovorus CACC736. Collectively, the complete genome sequence of the L. amylovorus CACC736 will aid in the development of functional probiotics in the animal industry.