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The complete chloroplast genomes of three Korean Echinochloa crus-galli accessions
Nah, Gyoungju,Im, Ji-Hoon,Kim, Jin-Won,Kim, Kyunghee,Lim, Jongsung,Choi, Ah Young,Choi, Ik-Young,Yang, Tae-Jin,Park, Tae-Seon,Lee, DoKyoung,Kim, Do-Soon Informa UK (Informa Healthcare) 2016 Mitochondrial DNA. Part A Vol.27 No.6
Complete chloroplast genomes of two Miscanthus species
Gyoungju Nah,Ji-Hoon Im,Soo-Hyun Lim,Kyunghee Kim,Do-Soon Kim 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
The complete chloroplast (cp) genomes of two Miscanthus species, M. sinensis and M. sacchariflorus, were sequenced and investigated for genes, genome size variation, and polymorphisms. There are 154 genes in both cp genomes, consisting of 122 coding genes, 40 tRNA genes, and 8 rRNA genes. The cp genome contains two inverted repeat (IR) regions, separated by large single copy (LSC) region and small single copy (SSC) region. 112bp indels in M. sinensis and 152bp in M. sacchariflorus were found mainly in LSC and SSC, which are responsible for 40 bp-difference in cp genome size in two species. Likewise, out of 94bp of SNPs, 88bp were found in LSC and SSC regions. Although gene number and sequence structure were quite well conserved, indel distribution and size were different in these two Miscanthus species.
Potential of thermal image analysis for screening salt stress-tolerant soybean (<i>Glycine max</i>)
Kim, Jin-Won,Lee, Tae-Young,Nah, Gyoungju,Kim, Do-Soon Cambridge University Press 2014 Plant genetic resources Vol.12 No.1
<P>Non-destructive high-throughput phenotyping based on phenomics is an emerging technology for assessing the genetic diversity of various traits and screening in breeding programmes. In this study, non-destructive measurements of leaf temperature and chlorophyll fluorescence were conducted to investigate the physiological responses of soybean (<I>Glycine max</I>) to salt stress so as to set up a non-destructive screening method. Two-week-old seedlings of soybean in the V2 stage were treated with 0, 12.5, 25, 50 and 100 mM NaCl to induce salt stress. Three parameters, photosynthesis rate, stomatal conductance and chlorophyll fluorescence, decreased significantly, while soybean leaf temperature increased by exhibiting a positive correlation with NaCl concentration (<I>P</I>< 0.001). Soybean leaf temperature increased significantly at 50 mM NaCl when compared with the untreated control, although no visual symptom was observed. We selected leaf temperature as a major physiological parameter of salt stress as its measurement is much easier, faster and cheaper than that of other physiological parameters. Therefore, leaf temperature can be used for evaluating the responses to salt stress in soybean as a non-destructive and phenomic parameter. The results of this study suggest that non-destructive parameters such as chlorophyll fluorescence and leaf temperature are useful tools for assessing the genetic diversity of soybean with regard to salt stress tolerance and to screen salt stress-tolerant soybean for breeding.</P>
Soo-Hyun Lim,Hae-Rim Park,Dong-Gil Kim,DoKyoung Lee,Gyoungju Nah,Do-Soon Kim 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
More than 300 Miscanthus accessions as a potential bioenergy crop were collected in Korea and their morphological traits were investigated at various growth stages. Among morphological traits, stem growth habit, the presence of awn in spikelet, and autumn new shoot are the most important key traits enabling to cluster Miscanthus accessions into M. sinensis and M. sacchariflorus groups. Miscanthus sinensis has bunch stem growth habit and awn in spikelet, and produces autumn new shoot, while M. sacchariflorus has scattering stem growth habit with no awn in spikelet and does not produce autumn new shoot. Interestingly, we found several Miscanthus accessions showing intermediate morphological traits. 7 M. sinensis accessions showed morphological traits similar to M. sacchariflorus and 17 M. sacchariflorus accessions showed morphological traits similar to M. sinensis. Flow cytometry and chromosome counting finally revealed 5 Miscanthus hybrids, suggesting that they are resulted from natural hybridization between M. sinensis and M. sacchariflorus. Therefore, these Miscanthus hybrids can be used to understand genetic recombination between these two Miscanthus species and our understanding may support future efforts for breeding new Miscanthus variety with high biomass productivity and environmental adaptability.
( Junki Lee ),( Jaekyung Chon ),( Jongsung Lim ),( Eun-kyoung Kim ),( Gyoungju Nah ) 한국육종학회 2017 Plant Breeding and Biotechnology Vol.5 No.3
Chloroplast genome sequencing has served as valuable source for developing DNA markers, including the authentication of plant material used for health supplement from its fraudulent materials. We sequenced and analyzed the chloroplast genome of Allium victorialis, a medicinal plant, to discover potential marker regions for the authentication from Veratrum patulum, an inedible toxic plant. Although we examined conventional barcode marker loci in chloroplast, matK and rbcL, there was a difficulty in aligning coding regions and determining PCR primer sequences in these two loci between A. victorialis and V. patulum, possibly due to the distant evolutionary relationship. Instead, we identified potential DNA markers that carry Insertion/Deletion (InDels) that are able to discriminate these two species around clpP, petB, petD, rpl22, and ycf2 loci. In this analysis, we demonstrated the possibility of developing potential DNA markers in the chloroplast genome other than conventional barcode markers, such as matK and rbcL. The potential DNA markers identified in this analysis will serve as useful tools for future authentication of Allium and Veratrum species.