당뇨병과 동반된 화농성 간농양의 임상상 및 예후인자

오은숙,강무일,이원영,오기원,임동준,이소영,이정민,고승현,김성래,안유배,손현식,윤건호,차봉연,이광우,손호영,강성구 대한당뇨병학회 2000 임상당뇨병 Vol.1 No.1

연구배경: 감염질환은 혈관질환과 함께 당뇨병의 중요한 사망원인의 하나이며, 혈당조절이 불량할수록 감염 빈도가 증가됨은 잘 알려져 있다. 간농양의 발생이 있어서도 당뇨병은 중요한 기저질환 중의 하나이다. 일반적으로 간농양의 가장 흔한 원인균은 Escherichi coli라고 알려져 있으나, 최근 당뇨병을 가진 간농양 환자에서 Klesiella pneumoniae의 검출이 증가되고 있다. 또 최근 연구에 의하면 K.pneumonie가 간농양의 가장 흔한 원인균이며, K. pneumoniae에 의한 간농양은 K.pneumoniae 이외의 균주에 의한 간농양보다 당뇨병과 밀접히 관련되어 있다고 보고된 바 있다. 이에 저자들은 간농양 환자를 대상으로 당뇨병환자의 빈도를 알아보고, 그 원인균 및 특징을 비당뇨병 환자들의 경우와 비교해 보고자 하였다. 방법: 1992년 1월부터 1999년 6월까지 방사선학적 검사 혹은 수술에 의해 간농양이 확인되거나, 경피적 천자나 수술을 통한 배농액 배양, 혹은 혈액 검사에서 원인균이 증명된 182명의 환자를 대상으로 후향적 임상고찰을 실시하였다. 결과: 화농성 농양을 가진 167명의 환자(남:94명, 여:73명)중 당뇨병을 가진 환자는 54명 (남:30명, 여:24명)으로 32.3%를 차지하였다. 환자의 평균나이는 당뇨군에서 62.1 ± 13.4세, 비당뇨군에서는 55.1 ±15.5세로 당뇨군에서 높았으며(p=0.0021), 남녀간의 평균나이 비교 시 두 군 모두 여성의 나이가 의미있게 높았다. 비당뇨군에 비하여 당뇨군의 경우 저알부민혈증의 빈도와 aspartate transaminase가 증가되어있었다. 원인균주는 K. pneumoniae (당뇨군54%, 비당뇨군 39.1%), E. coli(당뇨군 17.5%, 비당뇨군 18.8%), Pseudomonas aeruginosa, Streptococcus viridans, Enterococcus 순이었으며 두 군간의 의미는 차이는 없었으나 K. pneumoniae의 경우 다른 균종에 비해 단독감염의 빈도가 높았다(82.8%). 사망률은 당뇨군에서 16.7%, 비당뇨군에서 7.1%로 당뇨군에서 의미있게 높았으며(p=0.019), 당뇨군에서의 사망환자는 모두 패혈증이 동반되어 있었다. 당뇨환자의 사망군은 생존군에 비해 당뇨병 이환기간이 길고 혼합감염의 빈도가 높았다(p=0.046). 검사실 소견에서는 총 빌리루빈 및 알카리성 포스파타제의 증가가 관찰되어 있었다. 결론: E. coli가 간농양의 주된 원인균이라는 기존 보고와는 달리 당뇨군과 비당뇨군 모두 K. pneumoniae가 간농양의 주된 원인균이었다. 또한 당뇨 유병기간이 길고 당뇨조절이 불량한 환자에서 혼합감염이며 총 빌리루빈 및 알카리성 포스파타제의 증가등과 같은 위험인자가 존재할 경우 사망률이 증가될 수 있으므로 이들 군에서는 적극적인 치료가 필요할 것으로 사료된다. Background: Liver abscesses are commonly associated with underlying disease, particularly diabete mellitus. The number of the liver abscesses caused by Klebslella pneumoniae in diabetic patient has been increased in Korea nowadays. This study was conducted to clarify the clinical presentation and prognostic factors of pyogenic liver abscesses, especially in diabetic patients and to determine the proportion of K. pneunomiae as a pathogen in liver abscess in Korea. Methods: Medical records of 167 patients treated for pyogenic liver abscess from January, 1992 through June, 1999 were reviewed retrospectively in detail. Major Pathogenic organism, clinical manifestations, prognostic factors, the importance of diabetes mellilus as an underlying disease and its effect on clinical features and prognosis were analyzed. Results: Among 167 cases of pyogenic liver abscess, underlying diabetes mellitus was present in 32.3%. The mean age of patients was 62.7 13.4 years in diabetic liver abscess group and 55.1 15.5 years in non-diabetic liver abscess group. Most liver abscesses were cryptogenic in origin or secondary to the billary tree diseases. The clinical presentations among the two groups were not significantly different. When compared to patients without diabetes, patients with diabetes had significantly higher proportions of hypoalbuminemia and elevated aspartate transaminase level. The most common organism of the pyogenic abscess was K. pneumoniae in both groups. Percutaneous drainage of the abscess with appropriate antibiotics was the most commonly used therapeutic modality in liver abscess. The mortality rate of diabetic liver abscess is 16.1 % and 7.1% in nondiabetic liver abscess. Complications, especially septicemla, were found more frequently in patients with diabetes than in patients without diabetes (64.8% vs 28.3%, septicemia : 31.5% vs 9.7%). The factors influencing mortality in the diabetic liver abscess were disease duration, mixed infection, presence of septicemia, elevated bilirubin and alkaline phosphatase. Conclusions: In contrast to prior report that the E. coli was the most common pathogen in liver abscess, we found that K. pneumoniae was the most common organism cultured in liver abscess. Diabetic patients have more complications and higher mortality than patients without diabetes. Early detection and proper treatment are needed to improve the outcome for diabetic patient with liver abscess.

고도비만 환자에서 복강경 위소매절제술 후 발생한 누공의 내시경 치료 1예

류민선,심기남,조원영,김찬용,강현주,김미연,안소영,이윤표,조형원,정성애,이주호 이화여자대학교 의과학연구소 2013 EMJ (Ewha medical journal) Vol.36 No.2

Laparoscopic sleeve gastrectomy can reduce morbidity and mortality in patients with morbid obesity, but it can cause complications such as a gastrointestinal leak. A 30-year-old morbidly obese female who had type 2 diabetes mellitus and hypertension with estimated body mass index of 40.2 kg/m2 was admitted. Laparoscopic sleeve gastrectomy was performed. On postoperative day 19, a leak was suspicious on physical examination and radiologic findings. Conservative management was performed, but the patient was hemodynamically unstable and imminently septic. After laparoscopic drainage procedure, esophagogastroduodenoscopy was performed and revealed the fistula opening at staple line just below gastroesophageal junction. Fibrin tissue adhesive was injected around the fistula and the esophageal covered stent was inserted to cover the leak. At 14th days after stent insertion, the barium study confirmed no more leak. In this case, we experienced that the esophageal stent insertion with fibrin tissue adhesive injection may reduce recovery time of the fistula developed after laparoscopic sleeve gastrectomy

Development of salt-tolerant transgenic rice using OsCBF4 cDNA

So-Hyeon Baek,Eun-Mi Lee,Man-Kee Baek,Woo-Jae Kim,Jong-Ho Park,Ki-Yong Ha,Hyun-Soon Kim,Young-Chan Cho,Jeom-Ho Lee 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

This study was conducted to isolate a salt tolerant gene and to develop salt tolerant rice for reclaimed-saline areas through genetic transformation. A rice c/DRE binding factor 4(OsCBF4) cDNA was isolated from rice using RT-PCR. The full-length cDNA of the CBF4 gene consists of 1,429 nucleotides and 274 amino acid residues. In order to develop salt tolerant rice, transgenic rice plants containing the OsCBF4 gene were obtained via Agrobacterium-mediated transformation. The stable incorporation of the OsCBF4 gene into rice genome was confirmed by PCR and Southern analysis. The stable expression of introduced gene was also validated by RT-PCR analysis in T2 plants. Biological assay of T3 progeny of the transgenic plants in Yoshida solution containing 120mM Nacl for 2weeks, confirmed that the OsCBF4 confers salt tolerance to transgenic rice plants. OsCBF4 transgene in the transgenic line CBF4-10 was markedly expressed up to over three-fold in the leaf by 120 mM NaCl treatment. Real-time PCR analysis revealed that the levels of the transgene expression were markedly increased under salt treatment. The transgenic line CBF4-10 which showed highest ability to recover from the saline stress could be used as a potential source for salt tolerance in rice breeding programs

Oxygen-Releasing Microparticles for Cell Survival and Differentiation Ability under Hypoxia for Effective Bone Regeneration

Kim, Ho Yong,Kim, So Young,Lee, Hye-Young,Lee, Jin Ho,Rho, Gyu-Jin,Lee, Hyeon-Jeong,Lee, Hee-Chun,Byun, June-Ho,Oh, Se Heang American Chemical Society 2019 Biomacromolecules Vol.20 No.2

<P>Sufficient oxygen delivery into tissue-engineered three-dimensional (3D) scaffolds to produce clinically applicable tissues/organs remains a challenge for researchers and clinicians. One potential strategy to overcome this limitation is the use of an oxygen releasing scaffold. In the present study, we prepared hollow microparticles (HPs) loaded with an emulsion of the oxygen carrier perfluorooctane (PFO; PFO-HPs) for the timely supply of oxygen to surrounding cells. These PFO-HPs prolonged the survival and preserved the osteogenic differentiation potency of human periosteal-derived cells (<I>h</I>PDCs) under hypoxia. <I>h</I>PDCs seeded onto PFO-HPs formed new bone at a faster rate and with a higher bone density than <I>h</I>PDCs seeded onto phosphate buffered saline-loaded control HPs. These findings suggest that PFO-HPs provide a suitable environment for the survival and maintenance of differentiation ability of <I>h</I>PDCs at bony defects without vascular networks until new blood vessel ingrowth occurs, thus enhancing bone regeneration. PFO-HPs are a promising system for effective delivery of various functional cells, including stem cells and progenitor cells, to regenerate damaged tissues/organs.</P> [FIG OMISSION]</BR>

한반도 지체구조도에 대한 지구과학Ⅱ 교과서 분석

허현 ( Hyeon Heo ),이한솔 ( Han-sol Lee ),박소연 ( So-yeon Park ),정덕호 ( Duk-ho Chung ) 전북대학교 과학교육연구소 2021 과학과 과학교육 논문지 Vol.46 No.1

본 연구의 목적은 지구과학 Ⅱ 교과서에 제시된 한반도 지체구조도를 비교하기 위한 것이다. 이를 위하여 4종의 지구과학 Ⅱ 교과서에 실린 지체구조도를 비교했을 때 1종의 교과서가 나머지 3종의 교과서와 다르게 임진강대의 범위가 표시된 것을 확인하였다. 이를 위하여 한국지질자원연구원의 지체구조도(2020)를 지구과학 Ⅱ 과목 4종의 교과서들과 비교하는 기준 지체구조도로 설정하였다. 그 결과 1종의 교과서가 한국지질자원연구원의 새로운 지체구조도와 범위가 유사하였다. 현재 임진강대와 서해안 쪽에서 나오는 암석이 연장성 있게 발달을 하므로 이 부분을 좀 더 확대하여 홍성-임진강대로 새롭게 규정을 한 듯이 앞으로 새로 나오는 교과서에서는 이와 같은 점을 반영해야 할 것이다. The purpose of this study is to compare the structure of the Korean Peninsula’s geotectonic map presented in Earth Science II textbooks. For this study we compared four different Earth Science II textbooks that are now being used. After comparing the textbooks we found out that one textbook was different from the other three. So we compared the structure of the Korean Peninsula’s geotectonic map that has recently been published from the Korea Institute of Geoscience and Mineral Resources (2020) with the four Earth Science II textbooks. The results showed that the different textbook from the other three was similar to the geotectonic map that was recently published. Currently, rocks from the Imjingang Belt are extended to the west coast, and is now being called Hongseong-Imjingang Belt. So this should be reflected in the new textbooks.

Decadal-scale variations of sedimentary dinoflagellate cyst records from the Yellow Sea over the last 400 years

Kim, So-Young,Roh, Youn Ho,Shin, Hyeon Ho,Huh, Sik,Kang, Sung-Ho,Lim, Dhongil Academic Press in association with the Estuarine a 2018 Estuarine, coastal and shelf science Vol.200 No.-

<P>In recent decades, the Yellow Sea has experienced severe environmental deterioration due to increasing input of anthropogenic pollutants and consequently accelerated eutrophication. Whilst there have been significant advances in documenting historical records of metal pollution in the Yellow Sea region, changes in phytoplankton community structures affected by eutrophication remain understudied. Here, we present a new record of dinoflagellate cyst-based signals in age-dated sediment cores from the Yellow Sea mud deposits to provide better insight into eutrophication history and identification of associated responses of the regional phytoplankton community. It is worthy of note that there were significant variations in abundances and community structures of dinoflagellate cysts in three historical stages in association with increasing anthropogenic activity over the last 400 years. Pervasive effects of human interference altering the Yellow Sea environments are recognized by: 1) an abrupt increase of organic matter, including the diatom-produced biogenic opal concentrations (similar to 1850); 2) a distinct shift in phytoplankton composition towards dinoflagellate dominance (similar to 1940), and 3) recent acceleration of dinoflagellate cyst accumulation (similar to 1990). Particularly in the central Yellow Sea shelf, the anomalously high deposition of dinoflagellate cysts (especially Alexandrium species) is suggested to be a potentially important source of inoculum cells serving as a seed population for localized and recurrent blooms in coastal areas around the Yellow Sea. (C) 2017 Elsevier Ltd. All rights reserved.</P>

CPTED 활성화를 위한 범죄영향평가의 법제화 방안

박현호(Park Hyeon Ho),강소영(Kang So Young) 한국공안행정학회 2010 한국공안행정학회보 Vol.19 No.2

범죄에 대한 사후대응보다는 사전예방을 향한 보다 체계적, 과학적, 기술적 접근을 위한 노력의 일환으로서 환경설계를 통한 범죄예방(CPTED)은 점차 많은 정부기관, 국회, 학자, 그리고 산업의 연구, 관심과 투자의 대상이 되어가고 있다. 이에 CPTED 적용의 가장 중요한 초기 단계인 범죄위험성평가(crime risk assessment)에 대하여 국내의 환경영향평가법과 호주의 환경계획평가법을 비교법적 고찰을 통해 국내 실정에 적합하고 실현 가능한 범죄영향평가체계를 분석 및 도출하였다. 종합해보면 첫째, 많은 선진국들이 CPTED 관련 범죄분석과 범죄위험평가의 근거 법규를 갖고 있지만 호주 NSW주의 환경계획평가법이 가장 포괄적이고 체계적인 형태의 범죄영향평가의 법적 뿌리를 마련하고 있다고 분석되었다. 둘째, 국내에도 환경영향평가법이 있으며, 그 평가범위에 사회ㆍ경제환경에 대한 평가가 포함되어 있으나 범죄(안전)라는 사회환경 요소는 누락되어 있어 개선이 필요하다는 점이 발견되었다. 셋째, 호주 NSW주의 환경계획평가법에 의한 범죄영향평가체계를 기반으로 국내에서도 국민생활안전기본법안에 기초한 별도의 범죄영향평가 제도를 개발할 수 있으며, 최소한 현행 환경영향평가법에 있는 평가 범위를 개정하여 범죄영향평가를 제도화할 수 있을 것으로 파악되었다. 마지막으로 제도화를 통해 범죄영향평가체계가 마련되려면 대통령령, 부령, 한국산업표준 등에 의해 기준과 범위가 구체화되어야 하며, 실제 도시개발과 건축설계 등에 적용하기 위해서는 보다 구체화된 영향평가의 가이드라인과 매뉴얼 개발이 요구되는 것으로 확인되었다. Rather than reactive approaches proactive approaches to prevent crime in a more systematic, scientific and technological manner, CPTED is attracting more and more study, interest and investment from public agencies, MPs, academics and security industry in South Korea. In the context, this study elaborated on crime risk assessment as the first stage for CPTED in a comparative study between 'Environment Impact Assessment Act' in Korea and 'Environmental Planning and Assessment Act' in NSW, Australia. The study found that NSW's 'Environmental Planning and Assessment Act' has one of the most comprehensive and systematic crime impact assessment for CPTED in the world. Secondly, it was found that based on the NSW's Environmental Planning and Assessment Act we can include a crime impact assessment in the 'Environment Impact Assessment Act'. Thirdly, a crime impact assessment system can be introduced as the Community Safety Bill can support it. Finally, other relevant legislations and National Standards should specify the process and system of CPTED and also detailed risk assessment checklists and design guidelines/manuals have to be developed so that they can underpin the crime impact assessment laws.

Identification of candidate genomic region associated with seed longevity by re-sequencing in rice

In-Seon Jeong,Jun-Hyeon Cho,Chang-Deok Han,Hyun-Ju Lee,Hyeon-So Ji,Gang-Seob Lee,Ung-Han Yoon,Jang-Ho Hahn,Tae-Ho Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

It is well known that Dharial (Bangladesh origin and weedy rice line) has longer seed longevity than indica and japonica rice varieties. To study the genetic basis of seed longevity of Dharial, we developed 240 BC3F7 backcross recombinant inbred lines derived from the crosses between Dharial (a donor parent) and two korea rice accessions (recurrent parents) including Ilmi and Gopum, respectively. Among these lines, we selected two introgression lines with longer seed longevity and named them Ilmi-NIL and Gopum-NIL. Also, we developed an EMS-induced mutant line from Dharial which has shortened seed longevity, and named it Dharial-EMS. We performed re-sequencing of four rice accessions that are Dharial, Dharial-EMS, Ilmi-NIL, and Gopum-NIL. A total of 706×106 raw reads were generated which provided sequence data over 46x rice genome coverage per each accession. We did genome-wide variation analysis comparing produced re-sequencing data and the re-sequencing data of Ilmi from NABIC database with the Nipponbare reference sequence. By graphical analysis of SNP distribution in rice genome of the five accessions, we could select candidate chromosomal segments introgressed from Dharial in Ilmi-NIL and Gopum-NIL. The introgressed chromosomal segments were in seven regions in Ilmi-NIL and eight regions in Gopum-NIL, and four common introgressed regions between Ilmi-NIL and Gopum-NIL were identified. 2,758 SNPs between Dharial and Dharial-EMS were found in the introgressed regions. Also, we detected 450 genes including at least one SNP among these SNPs. This result will facilitate identification of genes and development of molecular markers for improvement of seed longevity.

일반연제 발표 : 젊은 한국 남성의 사구체질환에 대한 고찰

이상호 ( Lee Sang Ho ),유태현 ( Yu Tae Hyeon ),류승호 ( Lyu Seung Ho ),소병조 ( So Byeong Jo ),이현순 ( Lee Hyeon Sun ) 대한신장학회 2002 춘계학술대회 초록집 Vol.21 No.1

Wound-induced expression of the OsDof1 gene promoter in the Ds insertion mutant and transgenic plants

Hyemin Lim,Sung Han Park,A-Ram Kim,Hyeon-So Ji,Ung-Han Yoon,Tae-Ho Kim,Soo-Chul Park,Jang-Ho Hahn,Gang-Seob Lee 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

In plants, the Dof (DNA binding with One Finger) proteins are plant-specific transcription factors with a particular class of zinc-finger DNA-binding domain. The Dof genes have been predicted 30 different Dof genes in the rice Oryza sativa genome by phylogenetic analysis. The mostly Dof proteins contain a conserved region of 50 amino acids with a C2-C2 zinc finger motifs that binds a cis-regulatory element sequence 5’-T/AAAAG-3’. We found that a member of the DOF transcription factor family, Dof1 gene of rice, was expressed to wound from Ds insertion mutant population. Sequencing of the flanking regions of the transposon insertion site indicated that the gene-trap had been inserted near the front of the second exon of OsDof1 gene in chromosome 7. Genomic southern analysis revealed that mutant line contained a single copy of Ds gene trap. The Ds tagged rice mutant line, OsDof1::Ds, wound-inducible GUS expression was identified. To analyze the cis-acting elements, we constructed fusion genes with the OsDof1 promoter fused to the β-glucuronidase (GUS) reporter gene and transformed Arabidopsis and rice plants with these constructs. Wound-induced GUS expression was observed in the leaves of transgenic OsDof1::GUS rice and Arabidospsis plants. These results showed that, OsDof1 protein might be involved in stress responses and growth regulation in plant, might plays a role as a transcription regulator in stress response signal transduction pathways of plant.