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Jeong, Sin-Gu,Oh, Youn Seo,Joe, I-Seul,Jeong, So Young,Cho, Hyo Moon,Lee, Jun Sik,Oh, Won Keun,Cho, Tae Oh,Cho, Goang-Won ZOOLOGICAL SOCIETY OF KOREA 2017 ANIMAL CELLS AND SYSTEMS Vol.21 No.2
<P><B>ABSTRACT</B></P><P>The brown alga <I>Undaria pinnatifida</I>, which is called Mi-Yoek in Korea, has been traditionally consumed as a health food in East Asian countries. Recent studies have reported that <I>U. pinnatifida</I> has beneficial effects on arteriosclerosis, inflammation, fat metabolism, and tumors<I>.</I> In this study, we examined the anti-senescence effects of ethanol extracts of <I>U. pinnatifida</I> (UP-Ex) in human bone marrow mesenchymal stem cells (hBM-MSCs). UP-Ex protected hBM-MSCs against oxidative injury, as determined by MTT assays. This effect was confirmed by immunoblot analysis of the oxidation-sensitive protein p53 and the apoptotic protein cleaved caspase-3. Excessive intracellular reactive oxygen species (ROS) accumulation induced by oxidative stress was moderated in UP-Ex-treated hBM-MSCs (UP-Ex-MSCs). Similarly, expression of the ROS-scavenging enzymes superoxide dismutase 1 (SOD1), SOD2, and catalase was recovered in UP-Ex-MSCs. Excessive ROS induced by long-term cell expansion (passage 17) was significantly decreased along with restoration of the senescence proteins p53, p21, and p16 in UP-Ex-MSCs. UP-Ex treatment also improved the ability of these replicative, senescent hBM-MSCs (passage 17) to differentiate into osteocytes or adipocytes, suggesting that UP-Ex ameliorates the functional decline of senescent stem cells and may provide better therapeutic efficacy in stem cell therapy.</P><P><B>Abbreviations:</B> hBM-MSCs: human bone marrow mesenchymal stem cells; DCF: 2′,7′-dichlorodihydrofluorescein; DCFH-DA: 2′,7′-dichlorofluorescein diacetate; MTT: 3-(4,5-dimethylthiazol-2-yl-)2,5-diphenyltetrazolium bromide; PBS: phosphate-buffered saline; PFA: paraformaldehyde; RIPA: radioimmunoprecipitation assay; ROS: reactive oxygen species; SOD1: superoxide dismutase 1; SOD2: superoxide dismutase 2.</P>
Jeong, Sin-Gu,Lee, Jae-Joon,Kim, Ho-Tae,Ahn, Min-Ji,Son, Hee-Kyoung,Lee, Jun Sik,Oh, Won Keun,Cho, Tae Oh,Cho, Goang-Won The Basic Science Institute Chosun University 2018 조선자연과학논문집 Vol.11 No.2
The red algae Gracilaria vermiculophylla is widely spread around seaside areas across the globe, and has been used as a food resource in Southeast Asian countries. Previous studies have shown that Gracilaria red algae extracts have beneficial antihypercholesterolemic, antioxidant, anti-inflammatory, and antimicrobial effects. In this study, we investigated the antioxidant effects of Gracilaria vermiculophylla extracts (GV-Ex) on human bone marrow mesenchymal stem cells (hBM-MSCs). The acetone and DMSO/ethanol solvents of the tested GV contain higher total flavonoid and polyphenolic contents that can strongly scavenge reactive oxygen species (ROS). Pre-treatment with GV-Ex protected hBM-MSCs against oxidative stress induced by hydrogen peroxide treatment. The protective effects of GV-Ex treatment were confirmed by MTT assay. The elevated levels of ROS in hBM-MSCs caused by hydrogen peroxide induced oxidative stress were significantly decreased by GV extract treatment. The levels of the antioxidant proteins superoxide dismutase 1 (SOD1), superoxide dismutase 2 (SOD2), and catalase (CAT) were also restored or protected by GV-Ex treatment, suggesting that GV extracts moderate excess ROS levels and prevent cells from oxidative damage.
홍조류인 Gracilaria vermiculophylla 추출물에 의한 노화 골수유래 중간엽줄기세포의 항노화 및 분화능력 개선 효과
정신구(Sin-Gu Jeong),조태오(Tae Oh Cho),조광원(Goang-Won Cho) 한국생명과학회 2018 생명과학회지 Vol.28 No.9
홍조류인 꼬물꼬시래기(Gracilaria vermiculophylla)는 전 세계의 해변 지역에 널리 퍼져 있으며 아시아 국가에서 식량 자원으로 이용되어왔다. 이전 연구에 따르면, Gracilaria 속 홍조류 추출물에서 항산화 및 항염증 효과가 보고되었다. 본 연구에서는 노화된 인간의 골수 유래 중간엽 줄기세포(hBM-MSCs)를 이용하여 Gracilaria vermiculophylla 추출물(GV-Ex)의 항노화 효과를 조사하였다. MTT 분석와 immunoblot 분석(apoptotic protein p53과 cleaved caspase-3)을 이용하여, GV-Ex 전처리는 산화적 스트레스에 의해 손상된 hBM-MSCs의 세포생존력을 향상시킴을 확인하였다. 또, 세포내 생성된 ROS는 장기간 배양 된 MSCs (Passages 17; P-17)와 P-7 MSC에서 측정하여 서로 비교하였는데, P-17 MSC에서 증가되었고, GV-Ex 처리하면(GV-Ex treated P-17 MSCs) 유의하게 감소되었다. 또한, 항산화 효소인 SOD1와 SOD2, CAT의 발현 역시 GV-Ex 처리함에 따라 복원됨을 관찰하였다. 노화표지단백질인 p53와 p21, p16 등의 발현 또한 GV-Ex를 처리한 P-17 MSC에서 감소되었다. 줄기세포의 골세포(osteocytes) 혹은 지방세포(adipocytes)로 분화하는 능력 역시 GV-Ex를 처리한 P-17 MSCs에서 개선되었다. 이상과 같은 결과를 통해, GV 추출물은 노화된 줄기세포의 기능을 개선함을 시사한다. The red algae Gracilaria vermiculophylla is widespread on seashores worldwide and has been used as food in Asian countries. Previous studies have reported that extracts of Gracilaria red algae have beneficial anti-oxidant and anti-inflammatory effects. The present study examined the anti-senescence effects of Gracilaria vermiculophylla extracts (GV-Ex) in replicatively senescent human bone marrow mesenchymal stem cells (hBM-MSCs). GV-Ex pretreatment improved the cellular viability of hBM-MSCs that had been injured by oxidative stress. These effects of GV-Ex were confirmed by MTT assay and immunoblot analysis using the apoptotic proteins p53 and cleaved caspase-3. The reactive oxygen species (ROS) levels were examined in long-term cultured Passages 17 (P-17) mesenchymal stem cells (MSC) and compared to P-7 MSC. The ROS accumulation was greater in the P-17 than in the P-7. However, these increased ROS levels in the P-17 were decreased significantly after treatment with GV-Ex, and restoration of the levels of the anti-oxidant enzymes SOD1, SOD2, and CAT was also observed under these conditions. In addition, P-17 hBM-MSC treated with GV-Ex had decreased levels of the senescence proteins p53, p21, and p16. The results show that the ability of P-17 hBM-MSC to differentiate into osteocytes and adipocytes was improved by GV-Ex treatment, suggesting that GV-Ex ameliorates the functional decline of senescent stem cells.
한국 폐경여성에서 부갑상선 호르몬 유전자 BstBI 다형성과 골밀도 및 호르몬대체요법에 대한 골반응도 사이의 연관성
김정구 ( Kim Jeong Gu ),구승엽 ( Gu Seung Yeob ),김석현 ( Kim Seog Hyeon ),최영민 ( Choe Yeong Min ),문신용 ( Mun Sin Yong ),이진용 ( Lee Jin Yong ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.4
목적: 한국 폐경여성에서 PTH 유전자 다형성양상과 골밀도 및 호르몬대체요법후 골반응도 사이에 연관성이 있는가를 알아보고자 하였다. 연구 방법: 자연폐경여성 444명과 주기적 호르몬대체요법을 1년간 받은 자연폐경여성 309명을 대상으로 하여 PTH 유전자 BstBI 다형성양상을 RFLP으로 분석하였고 요추 및 대퇴 근위부에서의 골밀도를 호르몬치료전과 치료 후 1년에서 DEXA로, 혈청 CTX, osteocalcin, BAP, calcitonin, PTH Objective: To evaluate the relationship between parathyroid hormone (PTH) gene BstBI polymorphism, bone mineral density (BMD) and bone responsiveness to hormone replacement therapy (HRT). Methods: PTH BstBI polymorphism was determined by restriction fragm
신성철 ( Sin Seong Cheol ),김윤구 ( Kim Yun Gu ),백현정 ( Baeg Hyeon Jeong ),임영환 ( Im Yeong Hwan ),여호명 ( Yeo Ho Myeong ),김응호 ( Kim Eung Ho ),김정아 ( Kim Jeong A ),이방훈 ( Lee Bang Hun ),강우헌 ( Kang U Heon ),김범 ( Ki 대한신장학회 2003 Kidney Research and Clinical Practice Vol.22 No.3
A 21-year-old male was presented with sudden headache, fever, petechiae and neck stiffeness. The diagnosis of meningococcal meningitis was confirmed by examination of cerebrospinal fluid. The clinical symptoms of the illness were improved after treatment of antibiotics. However the paient developed generalized edema, oliguria, azoternia, and heavy proteinuria in the recovery phas of illness. Low serum C3 level was also noted. A kidney biopsy was perfromed and showed the features of postinfectious glomerulonephritis and typical subepithelial humps on electron-microscopic examination. His sysmptoms and laboratory findings were improved, and C3 level re turned to normal range after conservative treatment. We suggest that a complement deficiency should be reled out in patients of glomerulonephritis developed during the recovery phase of meningococcal men ingitis. C3 nephritic factor detection and renal biopsy should be carefully considered in these patients. (Korean J Nephrol 2003;23(3):321-325
경기,충청지역의 수치 산림입지도를 이용한 주요 수종의 산림생산력 추정에 관한 연구
구교상 ( Gu Gyo Sang ),김인호 ( Kim In Ho ),정진현 ( Jeong Jin Hyeon ),원형규 ( Won Hyeong Gyu ),신만용 ( Sin Man Yong ) 한국농림기상학회 2003 한국농림기상학회지 Vol.5 No.4
N/A This study was conducted to develop site index equations by main species grown in Gyunggi and Chungcheong provinces using environmental factors obtained from a digital forest site map. For this, 28 environmental factors were regressed on site index by species. Four to five environmental factors by species were selected as independent variables in the best site index equations (coefficients of determination greater than 0.91). For these site index equations, three evaluation statistics, mean difference, standard deviation of difference, and standard error of difference, were applied to the data set. Site index equations by species relationships developed in this study effectively estimate forest productivity in the study area. However, the site index equation of Larix leptolepis showed a larger than expected bias between the estimated and the measured site index. The reason is not clear in this situation, but might be because of the small sample set. It will be necessary, therefore, to conduct more studies to determine the exact reason. It is also expected that the site index equations with a few environmental factors as independent variables could provide valuable information about species well suited to given site conditions. Site index equations for other species should be developed to establish a rational policy about the selection of best species for site conditions.