http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
김시중,이영관,조원권 우송대학교 1996 우송대학교 논문집 Vol.1 No.-
Even though the tour business in Korea has grown rapidly with the changes of the time, it has caused lots of problems in the ethical aspects. Accordingly, the disagreements between tour business and society has been amplified and society requires new contract against tour business. By this reason. tour business is under an age of turning point that should solve comples ethical problems for themselves. To solve these ethical problems, sound business ethics must be established. To attain these goals, we should direct the study toward the basis of conceptual model of tour business. To do this, we have to seek after the ideology and spirits of business ethics developed by Yi Yi and Chung Yahk Yong who were representative scholar of Yi dynasty . We also have to set conceptual models in tour business ethics by adapting David Vogel's development model in business ethics, Joseph Badaracoo's business men's ethics model and David L. Whitney's determinant factors in tour business ethics. The conceptual model of tour business ethics represented by this study takes characteristic of basic research for the recognition of importance in tour business ethics and for the counter measure. By the consistent study, we must enhance tour business ethics and business image. we are going to ending up this study with the task of finding substantial alternatives that are essential to be an entrepreneur civilian internally and externally.
E. coli 과량발현을 통한 B형 간염 바이러스 preS1 유도체의 안정한 동위원소 N의 치환
박병관,김세하,한규훈,김선영,홍요정,이시형,한동설,김순종,박진구 木浦大學校基礎科學硏究所 1997 基礎科學硏究誌 Vol.15 No.-
We have used molecular biology technique to label the side chains and backbone of HBV preS1(1-56) with stable isotope (??) during E. coli over-expression and purified the protein to near homogeneity. The expression level of ?? labelled preS1(1-56) seems to be ∼15% of the total protein. to characterize the secondary structure of the labelled protein, circular dichroism was used. In different buffer conditions including pH 3.3 and pH 7.4 the protein seems to be devoid of any stable secondary structure probably suggesting that the N-terminal portion (preS1(1-56)) of HBV preS1 might be unstructured before binding to its target cells.
이광태,김성호,송시헌,김관태,김윤 대한신경외과학회 1992 Journal of Korean neurosurgical society Vol.21 No.4
The authors report a case of false aneurysm of the superficial temporal artery which manifested after operation for FCCD. An aneurysm of the superficial temporal artery is a rare disease. In collected literature, the incidence of superficial temporal artery aneurysms ranged from 0.5 to 2.5 percent of all aneurysms for which surgery was performed. False aneurysm should be suspected when a mass, which may or may not be pulsatile, appears in the scalp region after recent trauma. Troublesome(but not life-threatening) bleeding may occur if the lesion is unrecognized, and an attempt is made to "shell it out" as though it were an epidermal inclusion cyst.
공기청정 겸용 전열교환 환기유니트 적용시 실내공기환경 CFD 시뮬레이션
이정재,이시환,김용경,박성관 대한건축학회 2005 대한건축학회논문집 Vol.21 No.3
Recently, indoor air pollution problem is happening in apartment housing due to the use of new building materials that emit poisonous chemical such as Volatile Organic Compounds (VOCs) and Formaldehyde (HCHO) etc. Therefore, the issues about security of healthy dwelling environment is becoming important. There are the methods to reduce indoor air contaminant. First, source control which is the method to control the emission of contaminant from building materials. Second, adopting the air cleaning and ventilation system which is the method to dilute the concentration of contaminant and exhaust the contaminant to outside of the building quickly. In this research, we evaluated the improvement effect of the indoor air quality quantitatively by using CFD(Computational Fluid Dynamics) simulation on the assumption that air cleaning and ventilation system is installed.
이지연,양시용,고은지,이승구,김시관,조왕식,송민동 建國大學校 自然科學硏究所 2002 建國自然科學硏究誌 Vol.13 No.2
본 연구가 VRSA에 대한 신규 항생물질 생산 균주를 얻기 위하여 실시하였다. 국내외에 채취한 토양시료에서 분리한 방선균 중, 임상분리 VRSA와 돌연변이 유발 VRSA에 대해 항균성을 나타내는 JY-24를 선별하였다. 선발 균주는 원통형의 포자 모양을 갖고 있으며, Bennett's 배지에 배양시 aerial mycelium color는 흰색을 나타냈으며, reverse side color는 대체로 노란색을 나타내었다. 항균물질 생산을 위한 탄소원으로는 fructose, mannose, 질소원으로는 yeast extract가 가장 우수하였으며 glucose의 첨가로 항균 물질 생산성이 증가하는 것으로 나타났다. 최적 초기 pH는 pH 7로 나타났으며, 28 ℃, 37℃, 45 ℃의 온도에서 균이 성장하는 것으로 나타났다. 또한 배양기간에 따라 항균물질 생산성을 조사한 결과, 임상분리 균주에 대해 5일 배양 시 가장 높았으나, 3일 배양과 7일 배양 시 동일한 생산성을 나타내 생산속도가 빠르며, 안정한 물질인 것이 파악되었다. 향후 균주동정 및 항균물질 정제에 관한 연구를 수행하고자 한다. An antibiotic substance producing strain JY-24, effective to the VRSA (Vancomycin-resistant Staphylococcus aureus), was isolated from soil. The spore chain of JY-24 was cylindrical, and its sulface was smooth. The aerial mass color of the strain was white, and its reverse side color was yellow. The strain did not produce soluble pigment. For the production antibiotics, fructose, mannose and yeast extract were favorable as carbon and nitrogen sources. Optimal initial pH for the production of the antibiotic was pH 7. Accumulation of the antibiotic in the culture broth reached at maximal level after 5 days cultivation in Bennett's medium.
Kwan-Ho Park,Young-Cheol Choi,Jong-Gil Kim,Ji-Young Choi,Won-Tae Kim,Si-Kab Nho,Jae-Sam Hwang 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05
Protease from various sources have been studied biotecnologically. For biotechnological applications, one highly preferred enzyme is protease. There have been no reports of cloned genes encoding digestive proteases in the Laccotrephes japonenis, Ranatra unicolor, Muljarus japonicus. These insects are considered to be a predator of aquatic insects. RT-PCR was used to amplify cDNA fragments for digestive proteases from total RNA the hole body of the insects. The flanking sequences of the 5'- and 3'- end of the these genes were characterized by RACE-PCR. Sequence analysis showed that these genes contained complete ORF. The deduced amino acid sequences of these protease showed 62% identity to the serine protease of Creontiades dilutus, 58% to Lygus loneolaris trypsin-like serine proteinase, 54% to Triatonatoma infestans salivary trypsin. To generate Laccotrephes japonensis serine protease, the DNA fragment coding for serine protease is cloning into suttle vector pBACⅠ, named pBAC1-JG and infected to Spodoptera frugiperda (sf9) insect cell. The cDNA encoding JG was expressed as a 32-kDa polypeptide in baculovirus infected insect cells and the recombinant protein showed activity in the protease enzyme assay using gelatin as a substrate.
Kwan-Ho Park,Young-Cheol Choi,Jong-Gil Kim,Ji-Young Choi,Won-Tae Kim,Si-Kab Nho 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05
The black soldier fly, Hermetia illucens (BSF), has a worldwide distribution in the tropics and warm temperate regions and is active in the Korea from May through October. This species colonize a wide variety of decomposing vegetable and animal matter and oviposit in a variety of decomposing materials. In this study, how the BSF molting, adult emergence and mating rate changed by seasonal condition at the artificial rearing system was investigated. The black soldier fly larvae and pupae were reared under laboratory condition (27℃, 60% R.H.). Under the laboratory condition, molting and adult emergence were not influenced by seasonal factors such as climate, radiation intensity. But it is known that the sunlight is the most important factor of the mating. In the previous study the time of day, temperature, and humidity is significantly correlated with oviposition and mating. The rearing of BSF throughout the year is restricted by sunlight. In this study, the data shows definitely different mating numbers throughout whole year. The time of the day and sunlight density are changed with season and it influence on artificial rearing. To culture the black soldier fly throughout the year in Korea needs a more deep study under the artificial rearing system.
N-Acetylglycine Side Chain is Critical for the Antimicrobial Activity of Xanthostatin
KIM, SI-KWAN,UBUKATA, MAKOTO,ISONO, KIYOSHI 한국미생물 · 생명공학회 2003 Journal of microbiology and biotechnology Vol.13 No.6
This study was carried out to elucidate the mode of bacteriostatic property of xanthostatin (XS), a novel depsipeptide antibiotic with an N-acetylglycine side chain and selective antimicrobial activity against Xanthomonas spp. Two biotransformed XSs were isolated by the treatment of XS with the cell lysate of Xanthomonas campestris pv. citri, a solvent partition. prepatative TLC, and HPLC. Structure determination of those two biotransformed XSs demonstrated deletion of the N-acetylglycine side chain. Notwworthily, they showed no antimicrobial activity against Xanthomonas spp. This result suggests that the N-acetylglycine side chain plays a criticla role in the antimicrobial activity of XS, and that the bacteriostatic porperty of XS is susceptibility of the easter bond between the hexadepsipeptide nucleus and the N-acetylglycine side chain to hydrolytic enzyme(s) produced by Xanthomonas spp.