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동물의 장에서 분리한 Enterococcus sp.의 특성 및 분말화
박종진,변정수,조윤경,홍승서,이현수 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4
의약품 및 동물약품으로 사용되고 있는 분말유산균을 개발하고자 동물의 장에서 산성 pH 및 담즙산에 대한 내성이 강하고 대장균 생육억제력이 좋은 Enterococcus faecium L20을 분리 동정하고 이를 이용하여 고농도의 생균이 함유된 분말 유산균을 제조하였다. 분리된 균주는 pH 3.0에서 90% 이상의 내성과 0.3% 담즙산이 함유된 배지에서 100%의 내성을 나타냈으며 MRS 배지에서 대장균과 혼합배양시 24시간 이내에 대장균을 사멸시켰다. 분리된 균주를 산업용배지에서 배양한 후 동결건조시켜 분말을 만들었을 때 생균수는 5.0×0^11/g 이상이었다. 이것은 18℃에 보관하였을 경우 11개월 동안 80%의 생존율을 나타내었다. In order to develop a lactic acid bacterial powder which can be used as a probiotic for human and animal, a lactic acid bacteria which has high resistance against low pH and ox-gall, and shows a good growth inhibition against low pH and ox-gall, and shows a good growth inhibition against E. coli, was isolated from an animal intestine and characterized. The isolated strain was identified as Enterococcus faecium. It had more than 90% of survival at low pH for 2 hours and almost 100% of survival in the presence of 0.3% ox-gall. When co-cultured with E. coli in MRS broth, all of the E. coli cells were killed within 24 hours. The final powdered product of the isolated strain was manufactured after a freeze drying process suing an industrial media, and then checked its stability. Its storage stability was 80% for 11 months at 18℃.
糞尿와 都市 廢棄物과의 混合處理에 대한 硏究 (Ⅰ) : 肥料化를 中心으로한 實驗室的 考察 Experimental study for the fertilization
金熙鐘,金相烈,金秀生,孫晋彦,金燦國,成周慶,吳英敏 동아대학교 환경문제연구소 1977 硏究報告 Vol.1 No.1
우리나라에 있어서 都市廢棄物의 主인 糞尿와 固形廢棄物의 太宗인 無煙炭재를 同時에 效果的으로 處理할 수 있고 또한 堆肥化시킬 수 있는 好氣性 酸化方式의 最適條件을 얻기 위하여 生糞尿 및 煉炭재의 理化學的 및 生物學的 數値를 測定하고 이를 基礎로하여 上記 兩者를 混合處理 할수 있는 消化條件(溫度, 糞尿와 煉炭재의 配合比, 기타 固形物의 添加量, 注入되는 空氣量의 變化)에 따른 C.O.D., B.O.D.의 變化, 水分 및 電氣傳導度의 變化, 熱灼減量, N量의 變化 等을 硏究하였으며 이들 사이의 相關關係를 檢討하여 다음과 같은 結論을 얻었다. 1. 生糞尿에 固形廢棄物인 煉炭재나 紙類를 配合시켜 消化시키므로서 케이크속의 질소成分의 量을 增加 시킬수 있다. 2. 煉炭재의 配合은 糞尿의 消化速度를 增加 시킨다. 3. 糞尿의 處理를 위한 反應槽의 溫度는 50℃보다 60℃로 維持 시킬때 消化速度가 크다. 4. 反應槽의 溫度를 一定하게 했을때 空氣의 注入速度는 初期 反應時는 적게하고 後期 反應時는 크게 함이 效果的 이다. For the effective treatment and usable disposal of urban wastes in the area of Busan city, the composition of the nightsoil and anthracite bulky ash which is the main munic ipal solid wastes have been studied on the digestion of the compositive mixtures with the variable conditions that are ratio of compounding between nightsoil and ash, amount of solid additives and volume of air for the effective airation at 50, 65℃. The digestion has been detected by the physico-chemical and biological data; pH, specific gravity, content of moisture, C.O.D., B.O.D., electroconductance of the solution of the results, decrease of nition, and volume of total free nitrogen etc. The results are obtained as follows. 1) The characteristics of domestic nightsoil are different to the foreign data as table 5, but the data of anthracite ash almost are same except the composition of Fe₂O₃ and Al₂O₃. 2) Amount of total free nitrogen are increased by the digestion with the addition of solid waste, as effective absorption, to the nightsoil. 3) The digestion are accelerated by the compounding of ash, but the adding of sulfuric acid to fix the free ammonia reduced reaction. 4) The airation have to proceed slowly at initial step, and then endure enough volume of air at the later step.
김승수,곽병곤,김영대,김우경,김영균,이종국 白中央醫療院 2005 仁濟醫學 Vol.26 No.1
Objectives: The aim of this study was to describe the clinical characteristics of enuretic children and to evaluate the efficacy of treatments. Methods: Our study subjects enrolled were 52 children with nocturnal enuresis, who visited outpatient clinic of pediatrics in Seoul Paik Hospital and Ilsan Paik Hospital, during the period from October 1998 to August 2004. The data of gender, age, family history, symptom, clinical type of enuresis and drug therapy were collected and analyzed retrospectively. Results: The male : female ratio was 2.25 : 1. The mean age at diagnosis was 6.8 years with an age range from 4.3 years to 12.5 years. Fourty five cases(86.5%) were diagnosed as primary enuresis and 7 cases(13.5%) were diagnosed as secondary enuresis. A family history of enuresis was noted in 5 cases(9.6%) including 4 cases with parental enuresis history. The accompanying urinary symptoms were urgency(11.5%), frequency (7.7%), and dysuria(2.0%). Conclusion: We found frequent occurrences of enuresis in boys and primary type and an occasional association with a family history of enuresis. The two main treatment modalities were behavior control with an alarm system and drug therapy with imipramine, desmopressin, or oxybutynin. We recommend continuous drug medications to control nocturnal enuresis along with encouragement.
조종수,강병국,양재경,박종열,김윤근 진주산업대학교 농업기술연구소 2000 農業技術硏究所報 Vol.13 No.-
This paper was carried out to analyze the variation of composition of seed oil with storage periods. Difference of content of crude fat in fresh-cut seed and one year storage seed is not detected but in pentadecanoic acid, acid value, iodine value and peroxide value in one year storage seed have lower than those of the fresh-cut seed. Linoleic acid and oleic acid were high among of total fatty acid in fresh-cut seed but moiety of linolenic acid and icosanoic acid was low otherwise that of palmitic acid and oleic acid is increased in one year storge. In neutral lipid, capric and mytistic acid were high in fresh-cut, oleic acid, palmitic acid and linoleic acid were increased after one year storge. In glycolipid, the lauric acid and linoleic acid were high in fresh-cut seed, the palmitic acid was increased, otherwise, linolenic acid was decreased after one year storge. In phospholipid, palmitic acid and unknown part were high in fresh-cut seed, palmitic acid, oleic acid and linolenic acid were increased after one year storge.
Holography에 의한 Loudspeaker Cone의 振動에 관한 考察
李慶源,朴鍾允,林鍾洙,朴興秀 成均館大學校 科學技術硏究所 1984 論文集 Vol.35 No.1
Optical holographic interferometry is used to study the forced vibrational cone behaviour at some frequencies and photographs of resultant hologragm images are included. The entire cone vibrates almost uniformly in the low frequency region. Above certain frequency both transverse and longitudinal waves are coupled and together determine the vibration patterns. The coupling between longitudinal and bending waves gives rise to an antiresonance that the transverse amplitude become large. Bending waves only occur above the frequency at which this antiresonance occur. As the frequency increases, the resonance effect gradually progresses from the outer edge to the inner edge.
Prostaglandin E₂의 子宮內 投與가 家兎의 卵巢機能에 미치는 影響에 關한 實驗的 硏究
崔鏡淑,羅重烈,李壽鍾 고려대학교 의과대학 1983 고려대 의대 잡지 Vol.20 No.3
The effects of intra-uterine administration of Prostaglandin-E₂on the ovary and plasma steroid hormones were studied. Pseudopregnancy was induced in seven rabbits by the injection of 50㎍ of ethinyl estradiol and 100IU of hCG subcutaneously. And these seven pseudopregnant rabbits served as control group. In the prostaglandin-E₂ was implanted in uterus on Day seven under laparotomy and uterine incision with pentothal anesthesia. The plasma estrogen and progesterone level was determined by radioimmunoassay with three days interval in both control and prostaglandin=E₂ treated group. The uterus and ovaries were submitted to histologic study on Day 30. The following results were obtained; 1. Serum progesterone level in intra-uterine prostaglandin-E₂-implanted pseudopregnant rabbits was decreased at 14th day of pseudopregnancy, while the progesterone level of normal pseudopregnant rabbits was decreased from 15th day of pseudopregnancy and returned to normal values by 18th day. 2. Serum estrogen levels of pseudopregnant rabbit was higher than pretreatment state and returned to normal value by 30th day, while, in prostaglandin-E₂treated group, the serum estrogen values was decreased at 11th and 18th day below the level of pretreatment state and it was more decreased at 28th day. 3. The corpus luteum regresses from 15th day in control group, but in prostaglandin-E₂ Treated group, the corpus luteum was indistinct. The numbers of follicles in control group were 4-5 in number, while, in prostaglandin-E₂treated group, they were increased to 7-11 in number. The endometrium was papillary secretory patterns in control group, while, in prostaglandin-E₂ treated group, the endometrium was shown to have proliferative patterns.