Characteristics of progestin- insensitive early stage endometrial cancer and atypical hyperplasia patients receiving second-line fertility-sparing treatment

Shuang Zhou,Zhiying Xu,Bingyi Yang,Jun Guan,Weiwei Shan,Yue Shi,Xiaojun Chen 대한부인종양학회 2021 Journal of Gynecologic Oncology Vol.32 No.4

Objective: This study investigated the characteristics of progestin-insensitive endometrioid endometrial cancer (EEC) and atypical endometrial hyperplasia (AEH) patients receiving fertility-sparing treatments and assessed the therapeutic effects of second-line fertility- preserving treatments. Methods: Three hundred and thirty-eight patients with EEC (n=75) or AEH (n=263) receiving fertility-preserving treatment were retrospectively analyzed. ‘Progestin-insensitive’ was defined as meeting one of the following criteria: 1) presented with progressed disease at any time during conservative treatment, 2) remained with stable disease after 7 months of treatment, and/or 3) did not achieve complete response (CR) after 10 months of treatment. Clinical characteristics and treatment results of progestin-insensitive patients receiving second-line treatment and those of progestin-sensitive patients were compared. Results: Eight-two patients (59 AEH and 23 EEC) were defined as progestin-insensitive and 256 as progestin-sensitive. In multivariate analysis, body mass index ≥28.0 kg/m2 (odds ratio [OR]=1.898) and lesion size >2 cm (OR=2.077) were independent predictors of progestin- insensitive status. Compared to AEH patients, progestin-insensitive EEC patients had poorer second-line treatment responses (28-week cumulative CR rate after changing second-line treatment, 56.3% vs. 85.4%, p=0.011). No statistical difference was found in CR rate among different second-line treatments. Conclusion: Obesity and larger lesion size were independent risk factors associatedwith progestin-insensitive status. In progestin-insensitive patients receiving second-line treatment, EEC patients had lower CR rate comparing with AEH patients. Further study with larger sample size is needed to evaluate efficacy of different second-line treatments for progestin insensitive patients.

Modeling and Analyzing for Magnetic Field of Segmented Surface-Mounted PM Motors with Skewed Poles

Wang Huimin,Guan Peijun,Liu Shu,Wu Shuang,Shi Tingna,Guo Liyan 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.2

The skewed PM (permanent magnet) in the axial direction and the segmented PM in the circumferential direction, can reduce the cogging torque of the permanent magnet synchronous motor (PMSM). In this paper, the magnetization expression, which can be applied to diff erent circumferential sectioning modes, is derived. The quasi-3D magnetic fi eld analytical model suitable for diff erent circumferential and axial segmentation modes is proposed. The magnetic fi eld, cogging torque, back-EMF, and electromagnetic torque are obtained by the proposed modeling. The accuracy of the analytical method is verifi ed by 3D fi nite-element analysis. Finally, the modeling is used to analyze the variation of electromagnetic performance with diff erent radial skew angle and axial segments. The proposed modeling can shorten the calculation time, and analyze the variation law of motor performance with PM segmentation and skew comprehensively. It provides a reference for the design and optimization of segmented PMSM with skewed poles and has great signifi cance for improving motor performance.

Preparation of magnetic metal-organic frameworks with high binding capacity for removal of two fungicides from aqueous environments

Jiping Ma,Shuang Li,Gege Wu,Maryam Arabi,Feng Tan,Yafeng Guan,Jinhua Li,Lingxin Chen 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.90 No.-

A novel kind of Zr-based magnetic metal-organic frameworks (MMOFs) was prepared by immobilizationof UiO-66 onto Fe3O4@SiO2 particles via an efficient one-pot solvothermal method. Subsequently, it wasused for adsorptive removal of triclosan (TCS) and triclocarban (TCC) fungicides from aqueousenvironments by magnetic solid phase separation. Morphology and physical/chemical features of theMMOFs were fully characterized by XRD, SEM, TEM, FT-IR, and VSM etc., showing high specific surfacearea, appropriate functionality, and desirable magnetic property. Several main factors affecting theadsorption performances of TCS and TCC on the MMOFs were systematically investigated and optimized,such as pH value of water sample, amounts/types of adsorbent and salinity. Under the optimizedconditions, short adsorption equilibrium time (only 25 min) and outstanding saturated adsorptioncapacities (476.27 and 602.40 mg g 1 for TCS and TCC, respectively) were the remarkable superiorities ofthe MMOFs compared with that of most reported adsorbents. The MMOFs demonstrated excellentadsorption selectivity for TCS and TCC and anti-interference ability. Also, the reusability for at least 11cycles was another major profit of the MMOFs that saved cost and prevented waste. Moreover, theMMOFs demonstrated satisfactory removal/purification ability for actual environmental water samples. These benefits propounded a promising outlook of employing the MMOFs for influential removal ofpollutants with considerable reliability in thefield of wastewater treatment.

Initial Report on the Oriented-precipitation of T1-Phase in Creep-Aged Al-Cu-Li Single Crystal

Wei Hu,Jiqiang Chen,Shuang Han,Jinjun Xu,Jiale Miao,Ting Xing,Renguo Guan 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.5

In this work, the precipitation behavior of the T1-phase on each {111}Al habit plane was characterized by transmissionelectron microscopy (TEM), which was observed along two < 110 > Al zone axes in an Al-Cu-Li single crystal during creepaging. The results indicate that the oriented-precipitation of T1-phase in the Al-Cu-Li single crystal was originally determinedduring creep aging. The number densities of the T1-phase on the habit planes of (111)Al and (1−11)Al is noticeablyhigher than those of the T1-phase on the habit planes of (−111)Al and (11−1)Al in the same Al-Cu-Li single crystal when theexternal stress is loaded along the direction close to [111]Al orientation. It implies that the T1-phase tends to preferentiallyprecipitate on the habit planes that are perpendicular to the loading direction during creep aging. This work provides theinitial experimental evidence of the oriented-precipitation of the T1-phase in Al-Cu-Li alloy, and proposes the possibilityof regulating the precipitation distribution of the T1-phase.

Biological characterization of mesenchymal stem cells from bovine umbilical cord

Hui Xiong,Wei Jun Guan,Yue Hui Ma,Chunyu Bai,Shuang Wu,Yuhua Gao,Taofeng Lu,Qingyun Hu 한국통합생물학회 2014 Animal cells and systems Vol.18 No.1

Mesenchymal stem cells (MSCs) are multi-potential cells that are able to proliferate and differentiate into othercell types. Much research has been done on the MSCs from the umbilical cord (UCMSCs) in human, mice, andavian, but little literature has been published about these cells in big livestock. Here, we choose Luxi cattle asthe experimental animal, we describe an external culture of the UCMSCs from it and summarize the biologicalcharacteristics of these cells, e.g., morphologic appearance, surface antigens, colony-forming ability, geneexpression, and differentiation potential were detected via using immunofluorescence and reverse transcriptionpolymerase chain reaction (RT-PCR). The induced cells, osteoblast, lipoblast, hepatocyte, islet cells, andneurocyte were identified by Alizarin red staining, Oil-red-O staining, Periodic acid-schiff staining, andDithizone staining and RT-PCR detection for specific genes. Results suggest that biological characteristics ofthe UCMSCs were similar to those of MSCs previously analyzed. The primary UCMSCs were sub-cultured topassage 32, the UCMSCs expressed gene CD29, CD44, CD73, CD90, and CD166, induced cells illustratedtypical staining, and expressed specific genes, which indicate that the UCMSCs could be a novel alternativesource of MSCs for experimental and clinical applications.

Characterization of Two GAS1 Genes and Their Effects on Expression and Secretion of Heterologous Protein Xylanase B in Kluyveromyces lactis

( Zhao Lian ),( Ing Bo Jiang ),( Shuang Chi ),( Guo Hua Guan ),( Ying Li ),( Ji Lun Li ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.12

β-1,3-glucanosyltransferases play essential roles in cell wall biosynthesis in yeast. Kluyveromyces lactis has six putative β-1,3-glucanosyltransferase genes. KlGAS1-1 and KlGAS1-2 are homologs of Saccharomyces cerevisiae gene GAS1. RT-qPCR indicated the transcription level of KlGAS1-1 was significantly reduced while heterologous protein (thermostable xylanase B) secretion was enhanced during medium optimization. To evaluate if these two events were related, and to improve xylanase B secretion in K. lactis, we constructed KlGAS1-1 and KlGAS1-2 single deletion strains and double deletion strain, respectively. KlGAS1-1 gene deletion resulted in the highest xylanase B activity among the three mutants. Only the double deletion strain showed morphology similar to that of the GAS1 deletion mutant in S. cerevisiae. The two single deletion strains differed in terms of cell wall thickness and xylanase B secretion. Transcription levels of β-1,3-glucanosyltransferase genes and genes related to protein secretion and transport were assayed. The β-1,3-glucanosyltransferase genes displayed transcription complementation in the cell wall synthesis process. KlGAS1-1 and KlGAS1-2 affected transcription levels of secretion- and transport-related genes. Differences in protein secretion ratio among the three deletion strains were associated with changes of transcription levels of secretion- and transport-related genes. Our findings indicate that KlGAS1-1 deletion is an effective tool for enhancing industrial-scale heterologous protein secretion in K. lactis.

간접광고의 개선방향에 관한 인식 연구

이제영(Lee jei-young),관상(Guan shuang) 한국콘텐츠학회 2022 한국콘텐츠학회 종합학술대회 논문집 Vol.2022 No.7

Differentiation potential of neural stem cells derived from fetal sheep

Qian Li,Minghai Zhang,Wei Jun Guan,Shuang Zhang,Yanjie Zheng,Hebao Wen,Xiao Han 한국통합생물학회 2017 Animal cells and systems Vol.21 No.4

Neural stem cells (NSCs) are multipotent stem cells that can differentiate into many cell types in vitro. In this study, we isolated and established an NSC line from fetal Ovis aries. Based on the results of immunofluorescence staining, NSCs expressed Nestin, Pax6 and MAP2. Moreover, a reverse transcription–polymerase chain reaction assay was used to biologically characterize the cell line. NSCs were induced to differentiate into neurogenic cells in vitro. They expressed MAP2, glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP). In this study, we successfully isolated and cultivated NSCs from the hippocampal tissue of fetal sheep. NSCs not only displayed a self-renewal capacity but also had the potential to differentiate into neurons and glial cells. This study provided valuable experimental data for NSC transplant research.

Tissue distribution and functional characterization of odorant binding proteins in Chilo suppressalis (Lepidoptera: Pyralidae)

Sajjad Ali Khuhro,Hui Liao,Guan-Heng Zhu,Shuang-Mei Li,Zhan-Feng Ye,Shuang-Lin Dong 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.4

Odorant binding proteins (OBPs) play important roles in the insect olfaction and other diverse physiological processes. Forty OBP genes have been molecularly identified from Chilo suppressalis (Walker), a notorious rice pest in Asian countries, but little is known about the olfactory function for most of these genes. In the present study, we first determined the tissue expression profiles of 34 OBPs (excluding two general odorant bonding proteins (GOBPs) and four pheromone binding protein (PBPs)) by quantitative real-time PCR (qPCR), and found that 9 genes (OBP1, 3, 4, 11, 15, 17, 19, 20 and 24) were specifically or predominantly expressed in antennae of both sexes, suggesting their roles in olfaction, while three genes (OBP29, 30 and 32) were almost not expressed in antennae. Focusing on olfactory roles, the ligand specificities of six antenna specifically or predominantly expressed genes were further investigated for 35 plant volatiles, using the fluorescence competitive binding assays. The results revealed that six OBPs displayed different ligand preference, suggesting a differentiation of OBPs in ligand binding spectrum. Of six tested OBPs, OBP3, 11, 17, 19 and 31 showed moderate (Ki =10.21–19.85 μM) or high (Ki < 10.00 μM) binding affinity for 11 and one plant volatiles, respectively. In particular, a plant volatile β-ionone had high or moderate binding to all five OBPs. Our study suggests that these five OBP genes play important roles in the perception of different host plant volatiles, providing insight into the olfactory mechanism in C. suppressalis.

Detection and Characterization of PCR-SSCP Markers of the Bovine Lactoferrin Gene for Clinical Mastitis

Zhou, Lei,Yang, Yuan-Yuan,Li, Zhong-Hao,Kong, Li-Juan,Xing, Guan-Dong,Di, He-Shuang,Wang, Gen-Lin Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.10

A total of 80 cows, including 40 top mastitis resistant and 40 top mastitis susceptible animals as Group I and Group II, were selected from a population of 520 cows based on clinical mastitis occurrence. PCR-SSCP analysis on four fragments within the 5'region and two fragments of Exons 4,15 of bovine lactoferrin (bLF) revealed that four fragments-P1,P4,E4,E15-had polymorphisms which totally included six base mutations, and only two of them had significant differences in allele frequencies between resistant and susceptible groups, P1 (53.7% vs. 70.0%, p<0.05) and P4 (55.0% vs. 68.8%, p<0.05). Further study on these two promising markers combined with the milk performance traits of cows demonstrated that their selection would result in higher fat percentage (p<0.05), lower Somatic Cell Score (SCS) (p<0.05) and Clinical Mastitis Residuals (CMR) (p<0.01) indicating higher mastitis resistance and lower milk yield (p<0.05). The putative transcription factor binding sites in the 5'region were also studied by using MatInspector 7.2.2 software, and two signal pathways regulating the expression of bLF including the NF-${\kappa}B$ pathway and nuclear hormone receptor pathway were predicted.