Kirschner wire를 사용한 과두하 골절의 구강내 접근법

김성일,김승룡,백진아,고승오,신효근 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.3

The treatment of mandibular subcondylar fractures is a matter of controversy. The majority of mandibular subcondylar fracture are treated by closed reduction, but the displaced or dislocated mandibular subcondylar fractures may be treated by open reduction. The characteristics of open reduction are the anatomical reduction, the functional restoration, the rapid function, the maintenance of vertical ramus dimension, the better appearance and the less resultant TMJ problem etc. When an open reduction is considered, the wire, miniplate, lag screw and Kirschner wire are available with internal fixation. Of these, Kirschner wire is a simple method relatively and correct positioning of the wire achieves rigid fixation. But many open reduction methods for mandibular subcondylar fractures require extraoral approach. The extraoral approach has some problems, the facial scar and the risk of facial nerve injury. On the other hand, the intraoral approach eliminates the potency of the facial scar and the facial nerve injury, but is difficult to access the operation site. Since the intraoral approach was first described by Silverman (1925), the intraoral approach to the mandibular condyle has been developed with modifications. The purpose of this article is to describe the intraoral technique with the Kirschner wire on mandibular subcondylar fractures. Conclusion : The intraoral reduction with Kirschner wire on mandubular subcondylar fractures avoids the facial scar and facial nerve injury and is simple method to the extraoral approach. And it has minimal morbidity and better esthetics.

Bortezomib과 Dexamthasone으로 치료한 골수외 형질세포종 4예

백종현,이은영,장리라,손창배,신은경,서정아,이지숙,이호섭,이상민,신성훈,김양수 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Despite the use of aggressive local and systemic treatment including autologous stem cell transplantation in multiple myeloma, extramedullary recurrences are common and the prognosis of these patients is poor. Many novel drugs such as thalidomide, lenalidomide and bortezomib improve the response of treatment of multiple myeloma, but some reports failed to describe thalidomide has effect in extramedullary plasmacytoma. Recent data report on the successful treatment plasmacytomas with bortezomib in patients with advanced multiple myeloma. We treated 4 relapsed or refractory extramedullary plasmacytomas with bortezomib at our institution. We recognized all these extramedullary plasmacytomas decreased and showed more than partial response. This report lends support to the efficacy of bortezomib in the treatment of plasmacytoma and describes the safe use of bortezomib. Responses may, however, be of short duration. Therefore, despite our limited experience, we propose that bortezomib may be considered a therapeutic option for such patients who have risk of radiation therapy

<i>Ixeris dentata</i> (Thunb. Ex Thunb.) Nakai Extract Inhibits Proliferation and Induces Apoptosis in Breast Cancer Cells through Akt/NF-κB Pathways

Shin, Seong-Ah,Lee, Hae-Nim,Choo, Gang-Sik,Kim, Hyeong-Jin,Che, Jeong-Hwan,Jung, Ji-Youn MDPI 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.2

<P><I>Ixeris dentata</I> (Thunb. Ex Thunb.) Nakai (ID) exhibits various physiological activities, and its related plant derived-products are expected to represent promising cancer therapeutic agents. However, the anticancer effects of ID extract on breast cancer cells classified as estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) are still unknown. In this study, we investigated the anti-cancer effects and analyzed the molecular mechanism of ID extract in T47D, MCF-7 (ER-, PR-positive, HER2-negative), SK-BR-3(ER-, PR-negative, HER2-positive), and MDA-MB-231 (Triple-negative) through in vitro studies. Additionally, we examined its anti-tumor effects through in vivo studies. Our findings indicated that ID extract-induced apoptosis was mediated via various survival pathways on four breast cancer cells by identifying the factors including Bcl-2 family, phospho-Akt and phospho-nuclear factor-κB (NF-κB). Based on in vitro findings that induced apoptosis via Akt-NF-κB signaling, we investigated the effects of ID extract on mice bearing MDA-MB-231 cells. The results showed that ID extract significantly decreased MDA-MB-231 tumor volume and weight via inducing apoptosis by suppressing phospho-Akt. Overall, these results indicate that ID extract induces apoptosis through the Akt-NF-κB signaling pathway in MDA-MB-231 breast cancer cells and tumors, and it may serve as a therapeutic agent for triple-negative human breast cancer.</P>

Structure-Based Classification and Anti-Cancer Effects of Plant Metabolites

Shin, Seong-Ah,Moon, Sun Young,Kim, Woe-Yeon,Paek, Seung-Mann,Park, Hyun Ho,Lee, Chang Sup MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.9

<P>A variety of malignant cancers affect the global human population. Although a wide variety of approaches to cancer treatment have been studied and used clinically (surgery, radiotherapy, chemotherapy, and immunotherapy), the toxic side effects of cancer therapies have a negative impact on patients and impede progress in conquering cancer. Plant metabolites are emerging as new leads for anti-cancer drug development. This review summarizes these plant metabolites with regard to their structures and the types of cancer against which they show activity, organized by the organ or tissues in which each cancer forms. This information will be helpful for understanding the current state of knowledge of the anti-cancer effects of various plant metabolites against major types of cancer for the further development of novel anti-cancer drugs.</P>

The Prognosis of Patients with Primary Region Confined within Submucosa (yT0-1) after Neoadjuvant Chemoradiotherapy Followed by Radical Surgery for Advanced Lower Rectal Cancer

Seong Won Shin(신성원),Hee Cheol Kim,Jung Kyong Shin,Jung Wook Huh,Woo Yong Lee,Seong Hyeon Yun,Yong Beom Cho,Yoon Ah Park 대한외과학회 2021 대한외과학회 학술대회 초록집 Vol.2021 No.5

Apoptotic cell clearance in the tumor microenvironment: a potential cancer therapeutic target

Seong-Ah Shin,Sun Young Moon,Daeho Park,박종배,Chang Sup Lee 대한약학회 2019 Archives of Pharmacal Research Vol.42 No.8

Millions of cells in the human body undergoapoptosis not only under normal physiological conditionsbut also under pathological conditions such as infection orother diseases related to acute tissue injury. Swift apoptoticcell clearance is essential for tissue homeostasis. Defectiveclearance of dead cells is linked to pathogenesis of diseasessuch as inflammatory diseases, atherosclerosis, neurologicaldisease, and cancer. Significance of apoptotic cellclearance has been emerging as an interesting field fordisease treatment. Efficient apoptotic cell clearance playsan important role in reducing inflammation through thesuppression of inappropriate inflammatory responses underhealthy and diseased conditions. However, apoptotic cellclearance related to cancer pathogenesis is more complexin tumor microenvironments. Chronic inflammationresulting from the failure of apoptotic cell clearance cancontribute to tumor progression. Conversely, tumor cellscan exploit the anti-inflammatory effect of apoptotic cellclearance to generate an immunosuppressive tumormicroenvironment. In this review, focus is on the currentunderstanding of apoptotic cell clearance in the tumormicroenvironment. Furthermore, we discuss how signalingmolecules (PtdSer and PtdSer recognition receptor) mediatingapoptotic cell clearance are aberrantly expressed inthe tumor microenvironment and their current developmentstate as potential therapeutic targets for clinical cancertherapy.

Artificial Disc Replacement Combined With Fusion Versus Two-Level Fusion in Cervical Two-Level Disc Disease

Shin, Dong Ah,Yi, Seong,Yoon, Do Heum,Kim, Keung Nyun,Shin, Hyun Cheol Lippincott Williams Wilkins, Inc. 2009 1528-1159) Vol.34 No.11

STUDY DESIGN.: A prospective analysis. OBJECTIVE.: The purpose of this study was to compare the clinical and radiologic outcomes of cervical artificial disc replacement (C-ADR) combined with anterior cervical discectomy and fusion (ACDF) and 2-level ACDF in patients with 2-level cervical disc disease. SUMMARY OF BACKGROUND DATA.: Adjacent segment degeneration is a long-term complication of ACDF, and estimated to affect 25% of patients within 10 years of the initial surgery. Two-level fusion leads to a substantially greater increase in intradiscal pressure than one-level fusion. It has been demonstrated that C-ADR maintains motion at the level of the surgical procedure and decreases strain on the adjacent segments for prevention of adjacent segment degeneration. In the case of 2-level cervical disc disease, hybrid surgery (HS), consisting of C-ADR combined with ACDF, may be a reasonable alternative to 2-level ACDF (2-ACDF). METHODS.: Between 2004 and 2006, 40 patients undergoing 2-level cervical disc surgery at our hospital were identified who met the following surgical indications: 2 consecutive level degenerative disc disease between C3/4 and C6/7; either a radiculopathy or myelopathy; and no response to conservative treatment for >6 weeks. Twenty patients of the HS group were matched to 20 patients of the 2-ACDF group based on age and gender. Patients were asked to check the neck disability index (NDI) and grade their pain intensity before surgery and at routine postoperative intervals of 1, 3, 6, 12, and 24 months. Dynamic flexion and extension lateral cervical radiographs were obtained in the standing position before surgery and at routine postoperative intervals of 1, 3, 6, 12, and 24 months. The angular range of motion (ROM) for C2–C7 and adjacent segments were measured using the Cobb method with PACS software. RESULTS.: The HS group had better NDI recovery 1 and 2 years after surgery (P < 0.05). Postoperative neck pain was less in the HS group 1 month and 1 year after surgery (P < 0.05). There was no difference in arm pain relief between the groups. The HS group showed faster C2–C7 ROM recovery. The mean C2–C7 ROM of the HS group recovered to that of the preoperative value, but that of the 2-ACDF group did not (P < 0.05). The inferior adjacent segment ROM showed significant differences between the groups 6 and 9 months, and 1 and 2 years after surgery (P < 0.05, P < 0.01, P < 0.05, and P < 0.05, respectively). CONCLUSION.: HS is superior to 2-ACDF in terms of better NDI recovery, less postoperative neck pain, faster C2–C7 ROM recovery, and less adjacent ROM increase.

Induction of Apoptosis in Human Cancer Cells with Extracts of Taraxacum coreanum, Youngia sonchifolia and Ixeris dentate

Seong-Ah Shin,Hae-Nim Lee,Gang-Sik Choo,Ji-Youn Jung 한국실험동물학회 2016 한국실험동물학회 학술발표대회 논문집 Vol.2016 No.2

Anti‑cancer effects of lucidadiol against malignant melanoma cells

Shin Seong-Ah,Lee Jun Seob,Joo Byeong Jun,Ryu Gyoungah,Han Minjoo,Kim Huiji,An Jangeun,Koo Man Hyung,Youn Ui Joung,Lee Jun Hyuck,Park Hyun Ho,이창섭 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.5

Melanoma is one of the most aggressive and lethal skin cancers. Lucidadiol is a triterpenoid isolated from Ganoderma lucidum and is known to have various biological functions, including antibacterial effects. However, the anti-cancer effects and mechanism of action of lucidadiol in malignant melanoma are unknown. In this study, lucidadiol significantly reduced B16 melanoma cell viability in a dose- and time-dependent manner. In addition, lucidadiol induced apoptosis and suppressed cell mobility in B16 melanoma cells. Moreover, our findings revealed that lucidadiol remarkably downregulated phospho-Akt/ERK/JNK, but not p38. Taken together, our results suggest that lucidadiol could exerts its anti-cancer effects by inducing apoptosis via modulation of the Akt/MAPK pathway. Therefore, lucidadiol may be a potential cancer therapeutic agent for malignant melanoma.

Piperine에 의한 위암세포 AGS 증식 억제와 Apoptosis 유도

신성아(Seong-Ah Shin),이해님(Hae-Nim Lee),추강식(Gang-Sik Choo),김소정(So-Jung Kim),김형진(Hyeong-Jin Kim),박영석(Young-Seok Park),박병권(Byung-Kwon Park),김병수(Byeong-Soo Kim),김상기(Sang-Ki Kim),이후장(Hu-Jang Lee),정지윤(Ji-Youn 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.11

본 연구는 후추과 식물의 주성분인 piperine이 위암세포 AGS의 항암 효과에 미치는 영향을 확인하기 위해 수행되었다. Piperine에 의한 위암세포 AGS의 생존율을 확인하기 위해 MTT assay를 수행한 결과 농도 의존적으로 암세포의 생존율이 감소하는 것을 확인하였다. 이러한 암세포의 생존율 억제가 apoptosis에 의한 효과인지를 확인하기 위해 DAPI staining을 실시한 결과 piperine을 처치한 군에서 apoptotic body와 염색질 응축이 증가하는 것을 확인하였다. MTT assay와 DAPI staining의 결과를 바탕으로 piperine이 위암세포 AGS에서 apoptosis와 관련한 단백질 발현 양상에 미치는 영향을 확인하기 위해 western blotting을 실시하였다. 그 결과 piperine에 의해 AGS 세포에서 apoptosis를 유도하는 p53, Bax의 발현은 농도 의존적으로 증가하였고, apoptosis를 억제하는 Bcl-2, XIAP의 발현은 농도의존적으로 감소하였다. Apoptosis의 궁극적 단계인 caspase-9와 손상된 DNA를 복구하는 PARP의 분절은 증가하였으며, apoptosis를 방해하는 인자인 Akt의 인산화는 농도의존적으로 감소하였다. Akt 억제제인 LY294002와 piperine을 병행 또는 단독으로 처치하여 western blotting을 진행한 결과 Bcl-2의 발현은 piperine을 단독으로 처치했을 때와 유사한 감소 경향을 보였지만, p-Akt의 발현은 대조군과 비교해 LY294002와 piperine을 단독으로 처치했을 때보다 병행했을 때 더욱 감소하였다. 이와 반대로 Bax와 cleaved-PARP의 발현은 강하게 증가하였다. 본 연구의 결과를 종합해볼 때 piperine이 위암세포 AGS에서 Akt 경로를 통해 apoptosis를 유도하는 것으로 여겨지며, 향후 위암예방제나 치료제로의 개발 가능성이 있을 것으로 생각한다. Piperine [(E,E)-5-(3,4-methtylenedioxyphenyl)-2,4-pentadienolypiperidide] is a principal of Piperaceae, including Piper nigrum L. and Piper longum Linn., which has been used as a spice and traditional medicine. In this study, we investigated whether or not piperine has anti-cancer effects on AGS human gastric cancer cells. The results demonstrated that piperine not only inhibited proliferation using MTT assay but also induced apoptotic bodies using DAPI assay in a dose-dependent manner in response to piperine. Expression levels of p53, Bax (pro-apoptotic), cleaved caspase-9, and cleaved-PARP increased, whereas expression levels of Bcl-2, XIAP (anti-apoptotic), and Akt decreased in a dose-dependent manner compared with the control by western blotting analysis. To identify the connection between phospo-Akt and Bcl-2 family in response to piperine, LY249002 (Akt inhibitor) was treated with piperine (150 μM). The results were shown that expression of phospo-Akt was reduced whereas expression of Bax and cleaved-PARP increased in a dose-dependent manner. These results indicate that piperine induced apoptosis in AGS cells and may serve as a chemopreventive or therapeutic agent for human gastric cancer.