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연구보문 : 생태환경 ; 우분뇨 유래 젖소 유방염 저감을 위한 자외선 조사 살균의 효과 규명
김동혁 ( Dong Hyeok Kim ),임정주 ( Jung Ju Lim ),이진주 ( Jin Ju Lee ),장홍희 ( Hong Hee Jang ),장동일 ( Dong Il Jang ),이승주 ( Seung Joo Lee ),이후장 ( Hu Jang Lee ),민원기 ( Won Gi Min ),권순홍 ( Sun Hong Kwon ),김상훈 ( Sang H 한국환경농학회 2008 한국환경농학회지 Vol.27 No.4
E. coli, S. agalactiae, S. aureus는 젖소의 유방염을 유발하는 주요 원인균들이다. 이 균들은 분변 혹은 우유에 존재하며, 감염되지 않은 다른 개체로의 감염을 유발한다. 자외선은 소독제로서 이미 산업계 및 의료계에서 쓰레기 및 물의 살균에 사용되고 있으므로, 자외선을 이용하여 젖소의 유방염 확산을 방지하는 것의 실효성을 검증하였다. 분변의 함수율은 젖소 유방염 유발균의 증식에 영향을 미치지 않았고, 144시간 이상의 장시간 생존이 가능함을 확인 하였다. 자외선의 조사 시, 조사하는 동안 우분뇨 및 톱밥을 교반하는 것이 교반하지 않는 것보다 균의 증식을 억압하는 것을 확인할 수 있었다. 한편, 자외선의 살균력은 함수율이 낮을수록, 조사시간이 길수록, 조사거리가 짧을수록 더 증가하는 것을 확인할 수 있었다. 본 실험을 통해 얻은 함수율, 조사시간, 조사거리, 교반 여부에 대한 결과는 환경 유래, 특히 우분뇨 유래 젖소 유방염의 감염을 예방하고자 할 때 자외선 살균기의 적용이 가능할 것이며, 현장에 적용할 살균기의 제작에 중요한 기초 자료로서 활용될 것이다. Bovine mastitis is an important disease causing serious economic loss in dairy production and food poison in public health. The major causative agents of bovine mastitis include Escherichia coli (E. coli), Streptococcus agalactiae (S. agalactiae), Staphylococcus aureus (S. aureus). These bacteria were found in milk and environmental condition such as feces, water, soil and so on. Recently, many cases of mastitis are derived from environmental contamination of micro-organisms, which important factors for the spread of this disease in farm. Ultraviolet irradiation (UV) has been used as disinfection for waste and water in clinical and industrial facilities. Moreover the UV irradiation has been used as useful bactericidal agents to remove bacterial biofilms in environmental condition. In this study, we determined the bacterial replication in different percentage of water content (PWC) in sterilized saw dust and feces complexes from farm, and results showed that slightly decreased growth pattern of E. coli and S. agalactiae but increased growth pattern of S. aureus in various PWC (200, 400 and 600%) until 144 h incubation. In the bacteriocidal effect of UV irradiation to bacteria in saw dust and feces complex, the results showed that bacteriocidal effect was depended on the UV irradiation time, irradiation distance and PWC. Especially the antibacterial activity of UV irratiation is stronger in low PWC (50%), long time irradiation (50 sec), and short distance (5 cm) than other condition of this study. Furthermore UV irradiation with stirring showed increased the bactericidal effect compared without stirring. These results suggested that bovine mastitis causing agents may survive long time in environmental condition especially saw dust and feces complexes in farm and can cause a various disease including mastitis. Moreover, these data can be used as basis for application and development of UV disinfection to control of bovine mastitis from environmental contaminated bacteria in dairy farm.
Salmonella 방제에 대한 한방사료첨가제의 야외적용 효과
강호조,김용환,이후장,김종수,김도경,김은희,박미림,김곤섭,Kang, Ho-jo,Kim, Young-hwan,Lee, Hu-jang,Kim, Jong-shu,Kim, Toh-gyong,Kim, Eun-hee,Park, Mi-rim,Kim, Gon-sup 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.2
The present study was conducted to investigate the preventive effect of feed supplemented with 1.0% oriental herbal medicine feed additives (OHMFA) on the colonization of Salmonella spp. and incidence of death in broiler chickens. The frequency of Salmonella spp. in feces samples treated with OHMFA (25/239; 10.5%) was significantly reduced (p<0.05) than that of control group (83/347; 23.9%). A total of 108 Salmonella spp, belonging to four different serotypes, were isolated from three broiler farms. Among the serotypes isolated, Salmonella typhimurium was the moot prevalent (60.2%), followed by S. enteritidis (20.4%), S. gallinarum (13.0%) and S. pullorum (6.4%). In incidence of death in chickens during experiment, the mortality of rate OHMFA group (3.9%) was significantly lower (p<0.01) than that of control group (5.7%). These results show that the administration of OHMFA may prevent the colonization of Salmonella in chickens.
축산물 및 작업장 유래 Listeria monocytogenes의 혈청형, 약제감수성 및 plasmid profile
박상구,손원근,이후장,김용환,강호조,Park, Sang-koo,Son, Won-geun,Lee, Hu-jang,Kim, Young-hwan,Kang, Ho-jo 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.1
This study was carried out to investigate the serotype, and antimicrobial susceptibility and analyze the plasmid profile for the 145 isolates of L. monocytogenes isolated from livestock products and these product processing plants in Gyeongnam, Korea. All of L. monocytogenes strains belonged to serotype 1/2b (57.9%), 1/2a (20.0%), 4b (11.4%), 1/2c, 3b, 4c (each 2.9%) and 4d (0.7%). Serotype 1/2b, 1/2a, 4b from each source were found predominantly. Serotype 1/2b was predominantly higher than other serotype, and there was no significant difference between serotypes isolated from livestock products and product processing plants. 4b was major serotype isolated from raw milk and pork, and serotypes isolated from beef, chickens and slaughterhouse were 1/2b and 1/2a. The susceptibility of 145 strains of L. monocytogenes to 14 antibiotics commonly used in veterinary and human therapy was determined by disk diffusion method. All of L. monocytogenes strains were susceptible to amikacin, ampicillin, cephalothin, chloramphenicol, gentamicin, kanamycin, neomycin and penicillin. L. monocytogenes strains had the highest resistance with colistin (100%), oxytetracycline (44.8%), tetracycline (43.4%) followed by erythromycin (2.8%), spectinomycin (1.4%) and streptomycin (0.7%). Tetracycline resistance, and serotype distribution of the isolates from sample sources were significantly different. Resistance to at least one antibiotic was observed in all of them and 7 different resistant profiles were recorded. The most common resistance pattern were CL-OTC-TC (colistin-oxytetracycline-tetracycline) (42.8%). Among all tested isolates, two different plasmid profiles were observed. Of the 97 examined strains, 14 (14.4%) contained either the 8 and 11 kb plasmid or the 11 kb.
LC-MS/MS를 이용한 돼지 근육조직 중 dl-methylephedrine hydrochloride의 잔류 분석법 개발
최원석,김석,이후장,Chae, Won-Seok,Kim, Suk,Lee, Hu-Jang 대한수의학회 2020 大韓獸醫學會誌 Vol.60 No.4
This study examined the residue of dl-methylephedrine hydrochloride (MEP) on the muscle of pigs administered orally with MEP 12 g/ton feed for seven consecutive days. Twenty healthy cross swine were administered MEP. Four treated animals were selected arbitrarily to be sacrificed at 1, 2, 3, 4, and 5 days after treatment. MEP residue concentrations in the muscle were determined by liquid chromatography coupled with tandem mass spectrometry. The drug was extracted from muscle samples using 10 mM ammonium formate in acetonitrile followed by clean-up with n-hexane. The analyte was separated on an XBridgeTM hydrophilic interaction liquid chromatography column using 10 mM ammonium formate in deionized distilled water and acetonitrile. The correlation coefficient (R2) of the calibration curve was 0.9974, and the limits of detection and quantification were 0.05 and 0.15 ㎍/kg, respectively. The recoveries at three spiking levels were 94.5-101.2%, and the relative Standard Deviations was less than 4.06%. In the MEP-treated group, MEP residues on one day post-treatment were below the maximum residue limit in the muscle. The developed method is sensitive and reliable for the detection of MEP in porcine muscle tissues. Furthermore, it exhibits low quantification limits for animal-derived food products destined for human consumption.