측정방법에 따른 콩 성분의 항산화효과 비교

문갑순,권태완,류승희 한국콩연구회 2003 韓國콩硏究會誌 Vol.20 No.1

콩 성분의 항산화효과를 측정을 위해 DPPH법, FRAP법, TEAC법을 이용하여 이들 방법간의차이를 비교하고, 콩의 항산화효과를 측정하는데 가장 적합한 실험방법을 확립하고자 하였다. 콩의 유효 성분 중 항산화효과와 관련있는 isoflavone 및 phenolic acid의 표준품을 이용하여 항산화효과를 측정하였고, 항산화효과의 비교를 위해 Trolox, ascorbic acid, glutathione의 활성을 측정하였다. DPPH법, FRAP법, TEAC법의 3가지 항산화효과 측정방법에 따른 genistein의 효과가 가장 높았고 DPPH나 FRAP의 경우는 gentisic acid, syringic acid, ferulic acid 등의 phenolic acids의 활성이 높게 나타났고 isoflavones의 효과는 매우 낮게 나타났다. 항산화효과를 측정하기 위해 다양하게 이용되고 있는 방법들간에는 차이가 있음을 알 수 있었으며 TEAC법의 경우 glutathione 및 콩의 주요 성분인 isoflavones과 대부분의 phenolic acids의 활성을 평가할 수 있어 유용한 방법으로 여겨진다. To establish the proper assay for measurement of antioxidative activities in soybean, the main components relating to their antioxidative effects were measured by DPPH (α, α-diphenyl-β-pycryl-hydrazyl radical), FRAP (ferric reducing/antioxidant power), and TEAC (Trolox equivalent antioxidant capacity) assay. The antioxidative activities of commercial isoflavones (genistein, daidzein, and glycitein) and phenolic acids, which were well known as main antioxidative ingredients in soybean, were measured by three methods and compared the activities with Trolox, ascorbic acid, and glutathione as reference antioxidnats. By the FRAP and DPPH assay, gentisic acid, syringic acid and ferulic acid showed the strong antioxidative activities, while isoflavones and glutathione showed very low or undetectable activities . In contrast genistein, p-coumaric acid, and ferulic acid showed remarkable antioxidative activities by the TEAC assay. Therefore. it is proposed that TEAC assay is suitable for detecting antioxidative activity of soybean.

김치식이가 흰쥐의 항산화시스템에 미치는 영향

류승희,권명자,송영옥,송영선,문갑순 인제대학교 2003 仁濟論叢 Vol.18 No.1

김치는 배추를 주재료로 하여 마늘, 고추, 생강 등의 양념류를 포함하는 여러 재료들로 만들어지며 이들 재료 중에는 항산화효과를 가진 것으로 알려진 성분들이 많이 함유되어 있어 김치도 항산화효과를 나타낼 것으로 여겨진다. In vitro 실험계 및 세포 모델계에서 김치 재료 및 김치의 항산화효과는 확인된바 있으나 In vivo 시스템에서의 김치의 항산화효과에 관한 자료는 부족하다. 따라서 본 실험에서는 김치 섭취의 생체 내 항산화효과를 규명하기 위하여 흰쥐에게 동결건조한 김치 3, 5, 10% 첨가 식이를 6주간 섭취시키면서 간조직의 산화와 항산화효소계의 활성을 측정하였다. 간의 MDA 함량은 대조군에 비해 김치 투여군에서 낮았고 특히 5% 김치섭취군에서 현저히 낮은 값을 나타내었다. 단백질 산화를 나타내는 카르보닐 함량도 대조군에 비해 김치섭취군에서 낮아 생체 내에서 김치식이가 과산화물 생성 억제효과를 나타내었다. 항산화 효소계의 활성을 측정한 결과 SOD 활성은 대조군에 비해 김치섭취군에서 현저하게 높았고 김치첨가 함량이 증가할수록 SOD 활성이 유의적으로 높아져 10% 김치섭취군에서는 대조군에 비해 70% 이상 높은 활성을 나타내었다. Catalase 활성은 각 군에서 유의적인 차이가 나타나지 않았으나 GSH-px의 활성은 김치 섭취가 높을수록 상대적으로 높은 증가율을 나타내어 SOD 활성과 동일한 경향을 나타내었다. 따라서 김치식이는 생체 내에서 치질 및 단백질 과산화를 현저하게 억제하였고 항산화효소계인 SOD와 GSH-px의 활성을 농도의존적으로 증진시켜 김치식이가 체내의 산화적 스트레스를 효과적으로 억제하는데 기여함을 알 수 있었다. Kimchi is a Kruean traditional fermented food containing various ingredients such as Chinese cabbage, garlic, ginger, red pepper and fish sauce. Some of their components were known as its antioxidative activities and antioxidative effects of kimchi have been studied using in vitro and cell model system. To investigate the antioxidative effect of dietary kimchi, 4 groups of male S.D. rats (n=36) were fed 0, 3%, 5%, and 10% freeze-dried kimchi for 6 weeks and hepatic lipid peroxidation, protein oxidation and the activities of antioxidative enzymes were measured. Hepatic MDA content fed kimchi diet was lower than control diet. Protein carbonyl values were decreased in kimchi diet group, but not significant. SOD activities were significantly increased in kimchi added groups at dose-dependent manner. At 10% kimchi added group, SOD activity increased by 70% compared with control diet group. Catalase activities were not different between experimental groups and control group, whereas GSH-px activities were significantly increased in kimchi added group. As a result kimchi diet inhibited lipid and protein oxidation and induced antioxidative enzymes, SOD and GSH-px in rat hepatic tissue. It suggests that dietary kimchi is expected to prevent the oxidative stress in animal and human.

괄루근의 Glycosidase들에 대한 연구

류병호,양승택,조은정,김동수,문윤희,김희숙 慶星大學校 1996 論文集 Vol.17 No.2

Several glycosidase activities, α-galactosidase, β-galactosidase and β-fucosidase and their isozymes were founded in Trichosantes kirilowii root, Trichosantes radix. The activities of α-glucosidase, β-glucosidase and α-fucosidase were also assayed but were not detected. Those glycosidases were isolated and partial purified glycosidases by acid-treated Sepharose-6B, anion exchange Mono-Q HPLC, DEAE-Sephacel, butyl-toyopearl hydrophobic column chromatographies. However, these glycosidases need to be purified more homogeneous and characterized.

Best Practice 구현에 의한 신발 제조 자원관리 시스템

류영근,이태문,서승록,김행렬 한국의사결정학회 2000 경영과학연구 Vol.9 No.-

Presented in this paper is a development of ERP systems for footwear manufacturing. Final goal of the system development is to construct ERP system covering all works from order to make, shipment, accounting. And the objective of this paper is to introduce to completed 6 sub-systems of developing total 13 sub-system for production resource planning. In this study, introduce analysis of existing business flow and design of standard business process for development of systems. Also describe input and output of the standard business process for production resource management and basic structure, design of the total system flow, configuration of completed 6 sub-systems are presented. Finally, function of software modules and principal algorithms and basic data structuring techniques on the 6 sub-systems are explained.

공공스포츠센타 참가 주부의 레저만족과 생활만족의 관계

이승훈,배성민,문근석,유미진,이지훈 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.2

The purpose of this study was to define the relationship between housewife's practical physical education activity, leisure satisfaction and life satisfaction. For achieving the purpose, an analysis was made over the impact of attendance at public sports center on leisure satisfaction, the influence of attendance at public sports center on life satisfaction, the relationship of leisure satisfaction to life satisfaction for those who joined public sports center, and the impact of an experience of taking part in public sports center on life satisfaction. The instruments used in this study were Beard and Ragheb(1980)'s Leisure Satisfaction Scale(LSS) and Diener, Emmoson, Larsen, and Griffin(1985)'s Satisfaction with Life Scale(SWLS) adapted for this study. The statistical technique employed to analysis of variance and multiple regression analysis. The findings of this study were as below: First, the housewives who attended public sports center was significantly different in leisure satisfaction from one another according to personal characteristics. A significantly better leisure satisfaction was expressed by the group that was less educated, was older, had less than 3,000 thousand won of family income, was in very good health, chose golf, or responded to seeking more meaningful life. For job, the unemployed group was slightly higher in leisure satisfaction than the employed. Second, the personal characteristics also made a significant difference to the life satisfaction of the housewives who attended public sports center. Especially, a significantly higher life satisfaction was shown by the group that was older, had less than 3,000 thousand won of family income, was in very good health, chose golf was a little higher in life satisfaction than the unemployed. But education level generated no significant difference. Third, there appeared a close relationship between the housewives' leisure satisfaction and life satisfaction and life satisfaction who attended public sports center. Fourth, when the experience of attending public sports center was considered, the housewives with more than 3-year experience, who weren't expected to drop out in the practical physical education program, expressed significantly higher leisure satisfaction and life satisfaction. As this study found that the group that had participated in practical physical education program in public sports center for more than 3 years expressed better life satisfaction, it can be concluded that practical physical education can serve to raise housewife's leisure satisfaction and life satisfaction who joins public sports center.

안내염과 대장암이 동반된 재발성 간농양

전승정,김태헌,류민선,오다연,송명은,이신아,류재인,김혜인,문일환,유권 이화여자대학교 의과학연구소 2011 EMJ (Ewha medical journal) Vol.34 No.2

The causes of pyogenic liver abscess has been known as biliary tract disease or intrabadominal infection but the large proportions of the patients has no apparent underlying disorders. Recently colonic mucosal lesions were reported in patients with cryptogenic liver abscess and it has been suggested that colonic mucosal break may play a role in developing liver abscess in otherwise healthy patients. We experienced a patient of severe recurrent liver abscess complicated with endophthalmitis only 3 months after successful treatment of initial cryptogenic liver abscess and a polypoid colon cancer was discovered by chance. It seems prudent to proceed colonoscopic examination in patients with cryptogenic liver abscess especially when it is recurrent.

TV 스포츠프로그램에 관한 고찰

이승훈,배성민,문근석,유미진,이지훈 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.2

Nowadays, watching sports on TV is becoming a frame in the act of culture. Sports news, sports broadcast, and other programs that has relations with sports are developing not only with the quality of modern people but also with the relations of broadcasting company. This study aims at examining the way of studying TV sports programs and the influence of TV sports programs. It could be said that sports and television programs on sports have not been offered in-depth discussion and empirical exploration in the academic world so for, although their social influence is considerable. We firstly look over the meaning of mass media and then, we go into the study about mass media and sports. TV sports program takes social function, therefore, many theories is being used to study TV sports program. "Cultivation theory" and " Uses and gratification Approaches" are the main theories of the study on TV sports programs. At the first glance, the Cultivation Theory, which empirically analyzed the symbolic function of television as social ideological institution, and the Uses and Gratification Approaches, which contend the media audience are active rather than passive recipients, seem to be contradictory. However, if we look at the relationship between the mass media and audience not as one-way but as interactive process, it could be suggested that combining those two different theoretical worlds will be useful.

압출성형에 의한 국산 무연탄 활성탄의 세공구조 변화

이송우,문장천,김도한,이창한,최동훈,류동춘,송승구 부산대학교 환경문제연구소 2000 環境硏究報 Vol.18 No.-

The purpose of this study is to investigate the variation of pore size distribution of the coal-based extruded activated carbon. The extruded activated carbon was prepared by extruding the mixture of coal powder and coal tar. Pore size distribution of the extruded activated carbon was compared with that of the extruded coal and the extruded char. Also it was compared with that of the crushed activated carbon, raw coal and char. The extruded coal had coarser surface than that of raw coal. Macropores of the extruded coal were funned near 10,000Å in diameter during carbonization process and steam penetrated easily between particles and were growing between 3,000Å and 20,000Å in diameter during activation process.

콩 삶은 물을 첨가한 청국장 제조시 항산화활성 및 관련 성분들의 변화

이경희,류승희,이영순,김영만,문갑순 한국조리과학회 2005 한국식품조리과학회지 Vol.21 No.2

Soybean is an important plant as the source of protein and oil, as well as phytochemicals such as genistein, daidzein, phenolic acids, phytic acid, tocopherol, and saponin. Chungkukjang, a fermented soybean paste, is cammon in Korean meals and bacillus is usually used in the fermentation of steamed soybean. For its processing, whole soybeans are boiled in water until the beams are soft, and then the drained beans are wrapped with rice straw or starter and set in a warm place at 65℃ for 3~4 days. Normally, the remaining cooked water which was drained from the steamed beans is discarded. We supposed that this water possesses high amounts of useful components, and we therefore developed a modified method using the cooked water. After fermentation, we added the remaining cooked water which had been drained from the beans to the fermented soy beans and boiled them together. To investigate the bio-functionality of the modified chusngkukjang, the total antioxidative activity, isoflavones contents, phenolic acids, and 3-deoxyglucosone (3-DG) were measured at each stage of the preparation of changkukjang, The original and modified chturgkukjang possessed l a high antioxidative activity compared with the other samples, as did the drained water after steaming of the soybean. The contents of genistein, daidzein, and phenolic acids, which contained antioxidative activity, were also increased in the original chungkukjang and their contents were similar in the modified chungkukjang. The content of 3-DG was increased in the modified chungkukjang compare with the original. It is suggested that the active soybean components delivered to the drained water during the steaming process were useful for increasing the bio-functionality of the modified chunghnkjang.

말초성 신경아세포종 : 두개골 발생 증례 보고 Involving Skull

박양화,권익승,류연석,신미경,최문배,홍승관,문명선 대한신경외과학회 1988 Journal of Korean neurosurgical society Vol.17 No.4

The peripheral neuroblastomas are malignant neoplasms that originate from the adrenal gland and sympathetic nervous tissues. They usually occur in childhood. They exhibit such malignant features that they metastasize early to lung, liver, bone, rarely skull and other structures. The patients with peripheral neuroblastoma have short duration of symptom and short survival period. They reveal the characteristic light microscopic features that resemble other small cell carcinomas. The establishment of final diagnosis of peripheral neuroblastoma needs various special stainings for small cell carcinomas, and the electron microscopic findings are the most reliable. The authers recently experienced a case of peripheral neuroblastoma in a 26-year old man which involved right frontoparietal skull vault. The only chief complaint was a local non-tender mass at right frontoparietal scalp. Neither headache nor any neurological deficits was detected. Simple skull X-ary revealed a punched out rediolucency at right frontoparietal bone and brain CT showed a superficial elliptical high density mass that enhanced strongly. The mass was totally removed by wide craniectomy. The tumor invaded and penetrated the dura but the arachnoid membrane. at the tumor bed was not invaded by the tumor. The tumor was confirmed as peripheral neuroblastoma by various special stainings for small cell carcinomas. Following surgical resection of the mass, post-operative radiotherpy was offered(4800 rads for about 5 weeks). The patient aggrevated progressively and showed numerous metastases to such bones as lumbar vertebrae, pelvis and humerus to became paraplegic. 8 months after the operation, the patient died.