http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
The role of local IL6/JAK2/STAT3 signaling in high glucose-induced podocyte hypertrophy
( Hyung Ah Jo ),( Joo-young Kim ),( Seung Hee Yang ),( Seung Seok Han ),( Kwon Wook Joo ),( Yon Su Kim ),( Dong Ki Kim ) 대한신장학회 2016 Kidney Research and Clinical Practice Vol.35 No.4
Background: Interleukin-6 (IL6) is an important regulator of cellular hypertrophy through the gp130/Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathway. We tested the hypothesis that IL6 and its downstream gp130/JAK2/STAT3 pathway participated in high glucose (HG)-induced podocyte hypertrophy. Methods: IL6 levels in the media and lysates of podocytes were measured by enzyme-linked immunosorbent assay. Western blots were performed to determine the protein expression levels of gp130/JAK2/STAT3 among podocytes cultured with normal glucose (NG), NG + mannitol, NG + recombinant IL6, HG, and HG + IL6- neutralizing antibodies (IL6NAb). Immunoprecipitation was examined to determine whether gp130 interacted with JAK2 in response to HG or IL6. Podocyte hypertrophy was verified using protein/cell counts and flow cytometry. Results: IL6 levels were significantly increased in the media and lysates of podocytes cultured in HG compared with the NG groups. The nuclear phospho-STAT3/ STAT3 ratio was increased by HG and NG + IL6 and was attenuated in the HG + IL6NAb groups, indicating that nuclear STAT3 was activated following JAK2 and cytosolic STAT3 activation in response to IL6 secreted by HG-stimulated podocytes. Immunoprecipitation showed increased phospho-JAK2 recruitment to gp130 in the HG and NG + IL6 groups, and the addition of IL6NAb in the HG group significantly abrogated these increases. Podocyte hypertrophy was significantly increased in the HG and NG + IL6 compared with the NG condition and was diminished by the addition of IL6NAbs to the HG group. Conclusion: IL6 might play a prominent role in the local activation of JAK2/STAT3 in podocyte hypertrophy under HG conditions. In vivo studies examining this pathway are warranted. Copyright ⓒ 2016. The Korean Society of Nephrology. Published by Elsevier. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
S-538 The role of local IL6/JAK2/STAT3 signaling in high glucose-induced podocyte hypertrophy
( Hyung Ah Jo ),( Joo-young Kim ),( Seung Hee Yang ),( Seung Seok Han ),( Kwon Wook Joo ),( Yon Su Kim ),( Dong Ki Kim ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1
Background: IL6 is an important regulator of cellular hypertrophy through gp130/JAK2/STAT3. We tested the hypothesis that IL6 and its downstream gp130/JAK2/STAT3 signaling pathway were participated in high glucose (HG)-induced podocyte hypertrophy. Methods: Levels of IL6 in media and lysates of podocytes were measured by ELISA. WB were performed to determine protein expression levels of gp130/JAK2/STAT3 among podocytes cultured with normal glucose (NG), NG+mannitol (MN), NG+recombinant IL6 (rIL6), HG, and HG+IL6 neutralizing antibody (IL6NAb). Immunoprecipitation assay (IP) were examined to determine whether gp130 interact with JAK2 in response to HG or IL6. Podocyte hypertrophy was verified using protein/cell counts and flow cytometry. Results IL6 levels were significantly increased in the media and lysates of podocytes cultured in HG compared with the NG groups. Nuclear phospho-STAT3/STAT3 ratio was increased by HG and NG+IL6 treatment and attenuated by addition of IL6NAb to HG media, indicating that nuclear STAT3 activated following JAK2 and cytosolic STAT3 in response to IL6 secreted by HG-stimulated podocytes. IP showed increased phospho-JAK2 recruitment to gp130 in the HG and NG+IL6 groups, and IL6NAb in the HG group significantly abrogated these increases. Podocyte hypertrophy was significantly increased in HG and NG+IL6 conditions compared with NG. This effect was diminished by the addition of IL6NAb to the HG group.Conclusions: IL6 might play a prominent role in local activation of JAK2/STAT3 in podocyte hypertrophy under HG conditions.
Increased Microalgae Growth and Nutrient Removal Using Balanced N:P Ratio in Wastewater
( Seung Hoon Lee ),( Chi Yong Ahn ),( Beom Ho Jo ),( Sang Ah Lee ),( Ji Yeon Park ),( Kwang Guk An ),( Hee Mock Oh ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.1
Microalgal cultivation using wastewater is now regarded as essential for biodiesel production, as two goals can be achieved simultaneously; that is, nutrient removal efficiency and biomass production. Therefore, this study examined the effects of carbon sources, the N:P ratio, and the hydraulic retention time (HRT) to identify the optimal conditions for nutrient removal efficiency and biomass production. The effluent from a 2nd lagoon was used to cultivate microalgae. Whereas the algal species diversity and lipid content increased with a longer HRT, the algal biomass productivity decreased. Different carbon sources also affected the algal species composition. Diatoms were dominant with an increased pH when bicarbonate was supplied. However, 2% CO2 gas led to a lower pH and the dominance of filamentous green algae with a much lower biomass productivity. Among the experiments, the highest chlorophyll-a concentration and lipid productivity were obtained with the addition of phosphate up to 0.5 mg/l P, since phosphorus was in short supply compared with nitrogen. The N and P removal efficiencies were also higher with a balanced N:P ratio, based on the addition of phosphate. Thus, optimizing the N:P ratio for the dominant algae could be critical in attaining higher algal growth, lipid productivity, and nutrient removal efficiency.
Case Reports : A Case of Multiple Organ Failure due to Heat Stoke Following a Warm Bath
Seung Young Kim,Su Ah Sung,Gang Jee Ko,Chang Su Boo,Sang Kyung Jo,Won Yong Cho,Hyoung Kyu Kim 대한내과학회 2006 The Korean Journal of Internal Medicine Vol.21 No.3
Heat stroke is a potentially fatal disorder that`s caused by an extreme elevation in body temperature. We report here an unusual case of multiple organ failure that was caused by classical, nonexertional heat stroke due to taking a warm bath at home. A 68 year old diabetic man was hospitalized for loss of consciousness. He was presumed to have been in a warm bath for 3 hrs and his body temperature was 41℃. Despite cooling and supportive care, he developed acute renal failure, disseminated intravascular coagulation (DIC) and fulminant liver failure. Continuous venovenous hemofiltration was started on day 3 because of the progressive oligouria and severe metabolic acidosis. On day 15, septic ascites was developed and Acinetobacter baumanii and Enterococcus faecium were isolated on the blood cultures. In spite of the best supportive care, the hepatic failure and DIC combined with septic peritonitis progressed; the patient succumbed on day 25.
Ischemic Stroke after Use of Cannabis: a Case Report and Review of Literature
Jo Sung Jung,박예원,Seung Ah Lee,Tae Jin Song,Soo Mee Lim 대한자기공명의과학회 2018 Investigative Magnetic Resonance Imaging Vol.22 No.3
Cannabis or marijuana is the most commonly used recreational drug after alcohol in the world, and usage is generally recognized as having few serious adverse effects. However, usage is restricted in South Korea. The report of ischemic stroke associated with cannabis is rare in literature. We present a case of a 47-year-old female patient with no underlying disease presenting with acute ischemic stroke after smoking cannabis in South Korea. The result for synthetic cannabinoid metabolites (delta-9 tetrahydrocannabinol) screening was positive. Absence of other vascular risk factors and drug screening results suggest a causal role of cannabis in this ischemic stroke case. The patient eventually progressed to brain death. The underlying mechanism, clinical manifestation, and imaging findings of cannabis-related stroke will be reviewed.
CASE REPORT : Single Nodular Pulmonary Amyloidosis: Case Report
( Seung Hyun Lee ),( Young Chun Ko ),( Jong Pil Jeong ),( Chan Woo Park ),( Seok Ho Seo ),( Jong Taek Kim ),( Dae Won Park ),( Cheol Min Bak ),( Seung Ki Moon ),( Shin Hyoung Jo ),( Se Mi Kim ),( Ah L 대한결핵 및 호흡기학회 2015 Tuberculosis and Respiratory Diseases Vol.78 No.4
Amyloidosis is defined as the presence of extra-cellular deposits of an insoluble fibrillar protein, amyloid. The pulmonary involvement of amyloidosis is usually classified as tracheobronchial, parenchymal nodular, or diffuse alveolar septal. A single nodular lesion can mimic various conditions, including malignancy, pulmonary tuberculosis, and fungal infection. To date, only one case of nodular pulmonary amyloidosis has been reported in Korea, a case involving multiple nodular lesions. Here, we report and discuss the case of a patient having single nodular amyloidosis.
Single Nodular Pulmonary Amyloidosis: Case Report
Lee, Seung Hyun,Ko, Young Chun,Jeong, Jong Pil,Park, Chan Woo,Seo, Seok Ho,Kim, Jong Taek,Park, Dae Won,Bak, Cheol Min,Moon, Seung Ki,Jo, Shin Hyoung,Kim, Se Mi,Jung, Ah Lon The Korean Academy of Tuberculosis and Respiratory 2015 Tuberculosis and Respiratory Diseases Vol.78 No.4
Amyloidosis is defined as the presence of extra-cellular deposits of an insoluble fibrillar protein, amyloid. The pulmonary involvement of amyloidosis is usually classified as tracheobronchial, parenchymal nodular, or diffuse alveolar septal. A single nodular lesion can mimic various conditions, including malignancy, pulmonary tuberculosis, and fungal infection. To date, only one case of nodular pulmonary amyloidosis has been reported in Korea, a case involving multiple nodular lesions. Here, we report and discuss the case of a patient having single nodular amyloidosis.