Evaluation of an Automated Screening Assay, Compared to Indirect Immunofluorescence, an Extractable Nuclear Antigen Assay, and a Line Immunoassay in a Large Cohort of Asian Patients with Antinuclear Antibody-Associated Rheumatoid Diseases: A Multicenter

Jeong, Seri,Hwang, Hyunyong,Roh, Juhye,Shim, Jung Eun,Kim, Jinmi,Kim, Geun-Tae,Tag, Hee-Sang,Kim, Hyon-Suk Hindawi 2018 Journal of immunology research Vol.2018 No.-

<P>We assessed the diagnostic utility of the connective tissue disease (CTD) screen as an automated screening test, in comparison with the indirect immunofluorescence (IIF), EliA extractable nuclear antigen (ENA), and line immunoassay (LIA) for patients with antinuclear antibody- (ANA-) associated rheumatoid disease (AARD). A total of 1115 serum samples from two university hospitals were assayed using these four autoantibody-based methods. The AARD group consisted of patients with systemic lupus erythematosus (SLE), systemic sclerosis (SSc), Sjögren's syndrome (SS), and mixed connective tissue disease (MCTD). The qualitative results of all four autoantibody assays showed a significant association with AARDs, compared to controls (<I>P</I> < 0.0001 for all). The areas under the receiver operating characteristic curves (ROC-AUCs) of the CTD screen for differentiating total AARDs, SLE, SSc, SS, and MCTD from controls were 0.89, 0.93, 0.73, 0.93, and 0.95, respectively. The ROC-AUCs of combination testing with LIA were slightly higher in patients with AARDs (0.92) than those of CTD screen alone. Multivariate analysis indicated that all four autoantibody assays could independently predict AARDs. CTD screening alone and in combination with IIF, EliA ENA, and LIA are potentially valuable diagnostic approaches for predicting AARDs. Combining CTD screen with LIA might be effective for AARD patients.</P>

Changing Genotypic Distribution, Antimicrobial Susceptibilities, and Risk Factors of Urinary Tract Infection Caused by Carbapenemase-Producing Pseudomonas aeruginosa

Jeong Seri,Jeon Kibum,Lee Nuri,Park Min-Jeong,Song Wonkeun 대한진단검사의학회 2024 Annals of Laboratory Medicine Vol.44 No.1

Background: Carbapenem-resistant Pseudomonas aeruginosa (CrPA) is a leading cause of healthcare-associated urinary tract infections (UTIs). Carbapenemase production is an important mechanism that significantly alters the efficacy of frequently used anti-pseudomonal agents. Reporting the current genotypic distribution of carbapenemase-producing P. aeruginosa (CPPA) isolates in relation to antimicrobial susceptibility, UTI risk factors, and mortality is necessary to increase the awareness and control of these strains. Methods: In total, 1,652 non-duplicated P. aeruginosa strains were isolated from hospitalized patients between 2015 and 2020. Antimicrobial susceptibility, carbapenemase genotypes, risk factors for UTI, and associated mortality were analyzed. Results: The prevalence of carbapenem-non-susceptible P. aeruginosa isolates showed a decreasing trend from 2015 to 2018 and then increased in the background of the emergence of New Delhi metallo-β-lactamase (NDM)-type isolates since 2019. The CPPA strains showed 100.0% non-susceptibility to all tested antibiotics, except aztreonam (94.5%) and colistin (5.9%). Carbapenems were identified as a risk and common predisposing factor for UTI (odds ratio [OR]=1.943) and mortality (OR=2.766). Intensive care unit (ICU) stay (OR=2.677) and white blood cell (WBC) count (OR=1.070) were independently associated with mortality. Conclusions: The changing trend and genetic distribution of CPPA isolates emphasize the need for relentless monitoring to control further dissemination. The use of carbapenems, ICU stay, and WBC count should be considered risk factors, and aggressive antibiotic stewardship programs and monitoring may serve to prevent worse outcomes.

Genotypic Distribution and Antimicrobial Susceptibilities of Carbapenemase-Producing Enterobacteriaceae Isolated From Rectal and Clinical Samples in Korean University Hospitals Between 2016 and 2019

Jeong Seri,이누리,Park Min-Jeong,Jeon Kibum,Kim Han-Sung,Kim Hyun Soo,Kim Jae-Seok,송원근 대한진단검사의학회 2022 Annals of Laboratory Medicine Vol.42 No.1

Background: The emergence of carbapenemase-producing Enterobacteriaceae (CPE) represents a major clinical problem. Recently, the occurrence of CPE has increased globally, but epidemiological patterns vary across region. We report the trends in the genotypic distribution and antimicrobial susceptibility of CPE isolated from rectal and clinical samples during a four-year period. Methods: Between January 2016 and December 2019, 1,254 nonduplicated CPE isolates were obtained from four university hospitals in Korea. Carbapenemase genotypes were determined by multiplex real-time PCR. Antimicrobial susceptibility was profiled using the Vitek 2 system (bioMérieux, Hazelwood, MO, USA) or MicroScan Walkaway-96 system (Siemens West Sacramento, CA, USA). The proportions of carbapenemase genotypes and nonsusceptibility were analyzed using Pearson’s chi-square test. Results: Among the 1,254 CPE isolates, 486 (38.8%), 371 (29.6%), 357 (28.5%), 8 (0.6%), 8 (0.6%), and 24 (1.9%) were Klebsiella pneumoniae carbapenemase (KPC), oxacillinase (OXA)-48-like, New Delhi metallo-β-lactamase (NDM), imipenemase (IMP), Verona integron-encoded metallo-β-lactamase (VIM), and multiple producers, respectively. The predominant species was K. pneumoniae (72.6%), followed by Escherichia coli (6.5%). More than 90% of the isolates harboring KPC, NDM, and OXA-48-like were nonsusceptible to cephalosporins, aztreonam, and carbapenems. Conclusions: The impact of CPE is primarily due to KPC-, NDM-, and OXA-48-like-producing K. pneumoniae isolates. Isolates carrying these carbapenemase are mostly multidrug-resistant. Control strategies based on these genotypic distributions and antimicrobial susceptibilities of CPE isolates are required.

THE IMPACT OF PLATFORM CHANGE ON LIVE STREAMING PERSONAL MEDIA

Jeong, Seri,Lee, Yoo-jin,Park, Seung-ho 한국디자인학회 2015 한국디자인학회 학술발표대회 논문집 Vol.2015 No.10

Starting from how the live-internet-broadcasting system accommodates different platforms, this research will analyze the factors that contributed internet broadcasting’s mass popularity by taking closer look at an example: the MBC channel’s ‘My Little Television’. Using Lasswell’s SMCRE communication model as a standard for analysis, this research will examine the impact of media platform transition on the live streaming personal media. The results are as follows: Live streaming personal media is at an experimental stage of broadcasting media that is yet to fully satisfy the consumer needs. Live streaming personal media has to evolve in formats that utilize social networking service platforms in order to provide more choices for users to participate and co-create.

Performance comparison between Elecsys Anti-SARS-CoV-2 and Anti-SARS-CoV-2 S and Atellica IM SARS-CoV-2 Total and SARS-CoV-2 IgG assays

Jeong Seri,홍유라,Hwang Hyunyong 고신대학교(의대) 고신대학교 의과대학 학술지 2022 고신대학교 의과대학 학술지 Vol.37 No.2

Background: Although serological severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) tests from several manufacturers have been introduced in South Korea and some are commercially available, the performance of these test kits has not yet been sufficiently validated. Therefore, we compared the performance of Elecsys Anti-SARS-CoV-2 (ACOV2) and Anti-SARS-CoV-2 S (ACOV2S) and Atellica IM SARS-CoV-2 Total (COV2T) and SARS-CoV-2 IgG (sCOVG) serological tests in this study.Methods: A total of 186 patient samples were used. For each test, we analyzed the positive rate of serological antibody tests, precision, linearity, and agreement among the four assays.Results: The positive rates of COV2T, sCOVG, and ACOV2S were high (81.7%–89.2%) in total, with those for ACOV2S being the highest, while those of ACOV2 were as low as 44.6%. This may be related to the high completion rate of vaccination in Korea. The repeatability and within-laboratory coefficients of variation were within the claimed allowable imprecision; however, further research is needed to establish an allowable imprecision at low concentrations. COV2T showed a linear fit, whereas sCOVG and ACOV2S were appropriately modeled with a nonlinear fit. Good agreement was found among COV2T, sCOVG, and ACOV2S; however, the agreement between ACOV2 and any one of the other methods was poor.Conclusions: Considering the different antigens used in serological SARS-CoV-2 antibody assays, the performance of the tested assays is thought to show no significant difference for the qualitative detection of antibodies to SARS-CoV-2.

Performance Evaluation of the Roche Cobas 5800 HBV and HCV Tests: Comparison of the 200 and 500 μL Protocols

Jeong Seri,Lee Su-Kyung,Cho Eun-Jung,Kim Han-Sung,Lee Young Kyung,Kim Jae-Seok,Song Wonkeun,Kim Hyun Soo 대한진단검사의학회 2024 Annals of Laboratory Medicine Vol.44 No.3

Background: Clinical management of patients infected with hepatitis B virus (HBV) or hepatitis C virus (HCV) relies on the viral load (VL). The Cobas 5800 system (Roche Diagnostics) can determine VLs in 200 and 500 μL samples, but the performance of each protocol has not been compared. We evaluated the performance of both protocols for the HBV and HCV tests. Methods: Precision and linearity were verified using commercial panels. Probit analyses were used to determine limits of detection (LoDs). The results obtained with 336 samples were compared using the 200 and 500 μL protocols. Data from 6,737 retrospective HBV and 768 HCV samples were compared to estimate the effects of the different LoDs on the diagnostic results of the protocols. Correlations between protocols were tested with Spearman’s rank correlation coefficients (rho). Results: The precision and linearity of both protocols were verified. The LoDs for the 200 and 500 μL protocols were 6.5 and 2.7 IU/mL for HBV and 29.7 and 8.2 IU/mL for HCV, respectively. The agreement between the protocols ranged from 0.8 to 1.0. The results obtained with the HBV and HCV tests showed a strong correlation (rho=0.994). Only 0.4% of HBV and 0.4% of HCV test results were affected by the LoDs of the 200 μL protocol. Conclusions: The Cobas 5800 200 and 500 μL protocols for the HBV DNA and HCV RNA tests demonstrated excellent performance. These findings establish the 200 μL protocol as a new option for low-volume samples, especially for pediatric and difficult-to-bleed patients.

대용량 온디바이스 데이터를 활용한 Webix 웹 서비스 구현

윤여은(YeoEun Yoon),정세리(SeRi Jeong),전보광(BoKwang Jeon),윤동식(Dongsik Yoon),강정훈(JeongHoon Kang) 한국정보기술학회 2023 Proceedings of KIIT Conference Vol.2023 No.6

Rapid Identification of OXA-48-like, KPC, NDM, and VIM Carbapenemase-Producing Enterobacteriaceae From Culture: Evaluation of the RESIST-4 O.K.N.V. Multiplex Lateral Flow Assay

송원근,박민정,Seri Jeong,신동훈,Jae-Seok Kim,김현수,Han-Sung Kim,Nuri Lee,Jun Sung Hong,Seok Hoon Jeong 대한진단검사의학회 2020 Annals of Laboratory Medicine Vol.40 No.3

There is an urgent need for accurate and rapid diagnostic assays capable of identifying carbapenemase-producing Enterobacteriaceae (CPE). We assessed the performance of the RESIST-4 O.K.N.V. (OKNV) assay (Coris BioConcept, Gembloux, Belgium) for the identification of oxacillinase (OXA)-48-like-, Klebsiella pneumoniae carbapenemase (KPC)-, New Delhi metallo-β-lactamase (NDM)-, and Verona integron-encoded metallo-β-lactamase (VIM)-producing Enterobacteriaceae grown on sheep blood agar (SBA) and the CHROMagar KPC medium. Sixty-five carbapenem-resistant Enterobacteriaceae (CRE) isolates with characterized carbapenemase content were used to evaluate the OKNV assay. The assay correctly identified all 30 isolates that produced one of the four targeted carbapenemase families. Additionally, it correctly identified 15 isolates that co-produced KPC and NDM, VIM and NDM or OXA-48-like and NDM, but failed to identify an NDM-1 and OXA-232 co-producing Klebsiella pneumoniae isolate. All 16 non-carbapenemase-producing CRE and four CPE isolates exhibited negative results, and no cross-reaction was observed. Overall, the sensitivity and specificity of the assay were 97.8% and 100%, respectively. The OKNV assay is an accurate and rapid assay for identifying OXA-48-like, KPC, NDM, and VIM carbapenemases produced by Enterobacteriaceae isolates cultured on both SBA and the CHROMagar KPC media in the clinical microbiology laboratory.

COVID-19의 자가격리로 인한 영향

서유리(Yuri Seo),이정섭(Jeong-Seop Lee),김원형(Won-Hyoung Kim),김양식(Yang-Sik Kim),맹세리(Seri Maeng),최서현(Seo-Hyun Choi),배재남(Jae-Nam Bae) 대한사회정신의학회 2023 사회정신의학 Vol.28 No.2

목적 : 본 연구는 2019년 12월 중국 우한에서 발생한 코로나바이러스감염증-19(coronavirus disease-19, 이하 COVID-19)을 억제하기 위해 시행된 격리 조치 대상자들이 겪은 심리적인 영향을 조사하기 위해 시행되었다. 또한 이러한 심리적 영향과 자아존중감 사이의 연관성을 확인하고자 하였다. 방법 : 2022년 4월 25일부터 2022년 7월 14일까지 인천시 중구 정신건강복지센터에 COVID-19 자가격리 관리 대상자로 등록된 자에게 온라인으로 설문링크를 전송하였고, 이 중 설문에 응답한 사람을 806명을 대상으로 이들의 정신건강 특성을 연구하였다. 평가 도구로 자가 보고식 설문 Patient health questionnaire-9(PHQ-9), Generalized anxiety disorder-7(GAD-7), ISI-K(Korean version of the Insomnia Severity Index), Patient health questionnaire-15(PHQ-15), P4 suicidality screener scale(P4), Perceived stress scale(PSS), IES-R-K(The Korean Version of Impact of event scale-revised), RSES(Korean version of the Rosenberg Self-esteem scale)를 사용하였다. 위험군의 사회인구학적 요인의 차이를 알아보기 위하여 로지스틱 회귀분석을 시행하였다. 부정적인 정신건강 특성과 자아존중감과의 관련성을 확인하기위하여 Pearson 상관분석을 시행하였다. 결과 : 본 연구 결과 여성에서 우울감, 수면장애, 신체증상을 호소하는 비율이 높았으며, 20-30대 연령대에서 40-50대에 비하여 불안감이 높게 나타났다. 또한 20-30대 연령대에서 60대 이상에 비하여 신체증상을 호소하는 비율이 높게 나타냈다. 고학력자에서 자살사고, 신체증상 호소하는 비율이 낮았으며, 스트레스 척도는 여성에서, 그리고 미혼에 비해 결혼 또는 동거상태에서, 그보다 이혼 또는 별거상태에서 더 높게 나타났다. 자아존중감과 정신건강척도의 상관분석 결과, 우울, 불안, 수면장애, 신체증상, 자살사고, 신체증상, 외상 후 스트레스 장애 모두와 약한 정적 상관관계를 나타내었다. 결론 : COVID-19로 인한 격리를 경험한 대상자 중 정신건강 취약집단으로는 여성, 20-30대 연령층, 기혼 상태 또는 이혼이나 별거의 혼인상태, 고졸 이하의 학력자로 나타났으며 이들을 적극적으로 지원할 수 있는 정책의 수립이 필요할 것이다. Objectives: This study investigated the psychological impact experienced by individuals subjected to quarantine measures in response to the coronavirus disease-19 (COVID-19) outbreak that originated in Wuhan, China, in December 2019. Specifically, we examined the association between this psychological impact and self-esteem. Methods: From April 25 to July 14, 2022, an online survey was administered to 806 individuals registered as home-based treatment recipients at the Mental Health Welfare Center in Jung-gu, Incheon. Various assessment tools were used, including the Patient Health Questionnaire-9 (PHQ-9), Generalized Anxiety Disorder-7 (GAD-7), Korean version of the Insomnia Severity Index (ISI-K), Patient Health Questionnaire-15 (PHQ-15), P4 suicidality screener scale (P4), Perceived Stress Scale (PSS), Impact of Event Scale-Revised (IES-R-K), and Rosenberg Self-Esteem Scale (RSES, Korean version). Logistic regression analysis was conducted to examine the sociodemographic differences among the high-risk groups, and Pearson correlation analysis was performed to explore the relationship between negative mental health characteristics and self-esteem. Results: Depression, sleep disturbances, and physical symptoms were more prevalent among females than males. Increased anxiety was observed among individuals aged 20-30 compared to those aged 40-50. Furthermore, participants aged 20-30 reported higher rates of physical symptoms compared to those aged 60 and above. Participants with higher education exhibited lower rates of suicidal ideation and physical symptom complaints. Stress levels were higher among females and individuals who were married or cohabitating status than in single, and higher in those who were divorced or separated than married or cohabitating status. Correlation analysis demonstrated weak positive correlations between self-esteem and various mental health indicators, including depression, anxiety, sleep disturbances, physical symptoms, suicidal ideation, and post-traumatic stress disorder. Conclusion: Among individuals who experienced quarantine measures due to COVID-19, vulnerable groups in terms of mental health included females, individuals aged 20-30, married or divorced/separated individuals, and individuals with low educational attainment. These findings highlight the importance of actively supporting these groups.

전기차 충·방전 데이터 분석 기술 연구

방은진(EunJin Bang),정세리(SeRi Jeong),심별희(ByeoHee Choi),최효섭(HyoSub Choi) 한국정보과학회 2022 한국정보과학회 학술발표논문집 Vol.2022 No.12