소화기 ; 대전 지역에서 급성 A형 간염의 유전자형에 따른 임상 특성 고찰

이영우 ( Young Woo Lee ),양현웅 ( Hyeon Woong Yang ),이진아 ( Jin A Lee ),윤기호 ( Ki Ho Yun ),양성은 ( Seong Eun Yang ),이민지 ( Min Ji Lee ),박세영 ( Se Young Park ),김새희 ( Sae Hee Kim ),이향이 ( Hyang Ie Lee ),이윤정 ( Yun Ju 대한내과학회 2011 대한내과학회지 Vol.80 No.5

목적: B형 간염이나 C형 간염 등은 유전자형에 따른 차이가 알려져 있지만, 국내에서 증가하는 IIIA 유전자형과 기존의 IA 유전자형에 의한 간염의 차이가 거의 알려진 바 없다. 따라서 대전 지역에서 발생한 급성 A형 간염의 유전자형에 따른 차이를 알고자 한다. 방법: 2009년 11월부터 2010년 6월까지 급성 A형 간염으로 진단되면서 유전자형이 확인된 81명의 환자를 대상으로 임상적 소견들과 검사실 소견들을 전향적으로 연구하였다. 결과: 평균 나이는 32.6±7.4세이며, 평균 입원 기간은 7.7±2.4일이었다. 직업은 서비스직, 사무직 그리고 교육직이 가장 많았다. 유전자형 간의 급성 간염에 따른 증상들의 종류는 별다른 차이가 없었다. 유전자형에 따른 증상의 빈도는 IIIA 유전자형에서 구토와 설사가, IA 유전자형에서는 복통과 피부 반점이 상대적으로 많았다. 검사실 소견에서는 IIIA 유전자형에서 헤모글로빈의 평균, CRP 평균, AST 최대 수치 평균은 상대적으로 유의하게 높았다. 그리고 AST와 ALT 및 총 빌리루빈 최대 수치들과 INR의 수치가 IIIA 유전자형에서 상대적으로 높게 분포하는 경향을 보였다. 결론: 대전 지역에서 유행하는 급성 A형 간염에서 IIIA 유전자형에 따른 급성 간염은 기존의 IA 유전자형과 차이가 있을 것으로 보이며, 다기관 연구를 통한 심도 깊은 연구가 필요하겠다. Background/Aims: Acute viral hepatitis A is a major health problem in Korea and the influx of genotype IIIA is thought to be one reason. We examined the differences in the clinical characteristics and laboratory findings of genotypes IA and IIIA in Daejeon. Methods: From November 2009 to June 2010, 81 patients positive for IgM anti-HAV were enrolled prospectively. The hepatitis A was genotyped using real-time polymerase chain reaction. The clinical characteristics and laboratory results were compared on the Results: The mean patient age was 32.6±7.4 years. The mean hospitalization was 7.7±2.4 days. The patient occupation varied. Clinically, vomiting and diarrhea were relatively more prevalent in genotype IIIA than in IA. Abdominal pain and skin spots were relatively more prevalent in genotype IA than in IIIA. The hemoglobin, peak aspartate aminotransferase (AST) level, and C-reactive protein were statistically higher in genotype IIIA than in IA. The distributions of the peak AST, alanine aminotransferase (ALT) and total bilirubin values tended to be perched in genotype IIIA than in IA. The international normalized ratio (INR) tended to be slightly prolonged in genotype IIIA than in IA. Conclusions: Recently, genotype IIIA of acute viral hepatitis A has become prevalent in Daejeon. Hepatitis A genotype IIIA probably causes worse laboratory abnormalities than genotype IA.

수정-IPA를 통한 푸드카빙교육 효용성의 품질속성 분류에 관한 연구

이성주(Lee, Sung-Ju),임현철(Lim, Hyun-Cheo),김상미(Kim, Sang-Mi) 한국외식경영학회 2022 외식경영연구 Vol.25 No.2

본 연구는 푸드카빙 교육을 받은 수강생들이 지각하는 푸드카빙 효용성은 어떠한지 살펴보고, 어떠한 효용성들이 있고, 효용성의 품질 속성은 어떠한지 살펴보고자 하였다. 이러한 연구목적을 규명하기 위하여 푸드카빙 교육을 받은 경험이 있는 수강생을 모집단으로 설정하였고, 설문조사는 2020년 7월 20일부터 8월 20일까지 한 달간 진행되었다. 설문지는 총 500부를 배부하였고, 435부가 회수되었고, 불성실한 설문지 38부를 제외하고 397부가 최종 분석에 사용되었다. 분석결과, 중요 실행 요소로는 푸드카빙 교육은 지루하지 않고, 남녀노소 할 수 있고, 잡생각이 사라지고, 자존감이 높아지고, 자신의 가치가 높아지고, 마음이 편해지고, 우울함이 사라지는 등의 수업 자체에서 오는 만족감의 속성들이 포함되었다. 매력적 요소에는 푸드카빙 기술이 수익으로 연결되고, 강좌를 개설하고, 적은 금액으로 공방을 오픈하는 등의 수익 측면과 관련된 항목들이 포함되었다. 아울러, 기본적 요소에는 집중할 수 있고, 기분이 좋아지고, 자격증을 취득하고, 전문가가 될 수 있다는 측면의 항목들이 포함되었다. 이는 대체로 푸드카빙을 처음 배우는 수강생들이 기본적으로 가지는 목적들이 포함되었다. This study aims to examine the food carving utility perceived by students who received food carving education, the types of the utility, and its quality attributes. To investigate this research purpose, students who had received food carving education were set as a population, and the survey was conducted for one month from July 20 through August 20, 2020. 500 copies of the questionnaire were distributed in total, and 435 copies were collected. 397 copies were used in the final analysis, excluding 38 unreliable copies. As a result of the analysis, important execution factors included the attributes of satisfaction with the class itself, e.g., Food carving education was not boring; it could be done by men and women of all ages; it made cockeyed ideas disappear; it promoted their self-esteem; it enhanced their value; it comforted their hearts; and it made their depression disappear. Attractive elements included revenue-related items, such as food carving skills connected to profits; they could open lectures; and they could open a workshop with little money. Along with this, the basic elements included the following: They could concentrate; they could feel better; they could get a certificate; and they could become an expert. They usually included the purposes the students who would learn food carving for the first time had basically.

Novel amyloid precursor protein mutation, Val669Leu (“Seoul <i>APP</i>”), in a Korean patient with early-onset Alzheimer's disease

Bagyinszky, Eva,Kang, Min Ju,Van Giau, Vo,Shim, KyuHwan,Pyun, Jung-Min,Suh, Jeewon,An, Seong Soo A.,Kim, SangYun Elsevier 2019 NEUROBIOLOGY OF AGING Vol.84 No.-

<P><B>Abstract</B></P> <P>In this study, a novel mutation in <I>APP</I> gene, Val669Leu (“Seoul <I>APP</I>”), was reported in a Korean female patient with Alzheimer's disease. She developed cognitive decline at 56 years of age, and her memory declined rapidly over one-year period from her 1st visit to the hospital. Her Mini-Mental State Examination scores dropped from 25/30 to 13/30. Two years later, she developed parkinsonian features, myoclonic jerk, and generalized seizure. As the disease progressed, aggravated diffuse brain atrophy and small-vessel ischemic lesion was also observed, and she became mute and vegetative in 4 years from the symptom onset. Magnetic resonance imaging showed mild medial temporal lobe and hippocampal atrophy, and 18F-fluoro-deoxyglucose positron emission tomography showed bilateral temporoparietal hypometabolism. Plasma amyloid oligomer analysis revealed highly elevated Aβ oligomers levels in the proband patient. Family history revealed positive without biochemical confirmation because family members testified similar type of cognitive decline from the proband's mother and one of her aunt/uncle. Her half-siblings did not present any signs of memory impairment. Sanger sequencing of the proband patient revealed a novel mutation in <I>APP</I> gene, Val669Leu, but mutation was not found in her unaffected half-sisters. A designed algorithm by Guerreiro et al. on early-onset Alzheimer's disease–associated mutations suggested the mutation as possibly pathogenic mutation. On the other hand, PolyPhen2 and SIFT tools suggested as otherwise. Since the mutation was located nearby the β-secretase cleavage site of APP, right next to the Swedish APP (Lys,Met670/671Asn,Leu) mutation, it was named as “Seoul <I>APP”</I> mutation. 3D modeling revealed that this mutation could result in significant changes in loop orientation of APP and also its intramolecular interactions. Hence, a novel <I>APP</I> Val669Leu mutation could alter the binding interactions between APP and β-secretase, which may influence the Aβ40 and Aβ42 generations.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel variant in APP, Val669Leu, was found in a Korean patient, named Seoul APP. </LI> <LI> Proband patient developed disease phenotype in her 50s. </LI> <LI> Family history may be positive, and mutation may segregate with disease. </LI> <LI> Mutation is located nearby the beta-secretase site of APP, and potentially disturbs the enzyme mechanism. </LI> </UL> </P>

<i>Scutellaria baicalensis</i> Extracts and Flavonoids Protect Rat L6 Cells from Antimycin A-Induced Mitochondrial Dysfunction

Im, A-Rang,Kim, Young-Hwa,Uddin, Md. Romij,Lee, Hye Won,Chae, Seong Wook,Kim, Yun Hee,Jung, Woo Suk,Kang, Bong Ju,Mun, Chun Sun,Lee, Mi-Young Hindawi Publishing Corporation 2012 Evidence-based Complementary and Alternative Medic Vol.2012 No.-

<P>Antimycin A (AMA) damages mitochondria by inhibiting mitochondrial electron transport and can produce reactive oxygen species (ROS). ROS formation, aging, and reduction of mitochondrial biogenesis contribute to mitochondrial dysfunction. The present study sought to investigate extracts of <I>Scutellaria baicalensis</I> and its flavonoids (baicalin, baicalein, and wogonin), whether they could protect mitochondria against oxidative damage. The viability of L6 cells treated with AMA increased in the presence of flavonoids and extracts of <I>S. baicalensis</I>. ATP production decreased in the AMA treated group, but increased by 50% in cells treated with flavonoids (except wogonin) and extracts of <I>S. baicalensis</I> compared to AMA-treated group. AMA treatment caused a significant reduction (depolarized) in mitochondrial membrane potential (MMP), whereas flavonoid treatment induced a significant increase in MMP. Mitochondrial superoxide levels increased in AMA treated cells, whereas its levels decreased when cells were treated with flavonoids or extracts of <I>S. baicalensis</I>. L6 cells treated with flavonoids and extracts of <I>S. baicalensis</I> increased their levels of protein expression compared with AMA-treated cells, especially water extracts performed the highest levels of protein expression. These results suggest that the <I>S. baicalensis</I> extracts and flavonoids protect against AMA-induced mitochondrial dysfunction by increasing ATP production, upregulating MMP, and enhancing mitochondrial function.</P>

The association between prion proteins and Aβ1-42 oligomers in cytotoxicity and apoptosis

Hyeon, Jae Wook,Kim, Su Yeon,Park, Jun Sun,Choi, Bo Yeong,Lee, Sol Moe,Ju, Young Ran,An, Seong Soo A.,Kim, Chi-Kyeong Elsevier 2012 Biochemical and biophysical research communication Vol.424 No.2

<P>Misfolding of prion protein (PrP to PrPSc) can cause neurodegenerative prion diseases. As a glycosylphosphatidylinositol (GPI)-anchored membrane protein, the normal form of PrP (PrPC) can function as a receptor for ligands in the extracellular space. PrPC was suggested to be involved in memory, synaptic neuronal communication, and anti-oxidation as a neuroprotective agent. The recently identified interaction between PrPC and Aβ(1-42) oligomers suggested another role for PrP as a receptor for Aβ(1-42) oligomers, thereby influencing cytotoxicity and apoptosis. Here, the association between PrPC and Aβ(1-42) oligomers was investigated by visualizing protein localization in neuronal cells by immunocytochemistry. Aβ(1-42) oligomer-induced cytotoxicity was tested in respective expressions of PrPC by using mouse neuroblastoma-2a (N2a) cells, the prion protein overexpressed cells (L2-2B1), and a Prnp-null mouse hippocampal cell line (HpL 3-4). Moreover, apoptotic proteins such as caspase-8 were used to assess the effect of PrPC on Aβ(1-42) oligomer-mediated apoptosis. In L2-2B1 and HpL 3-4 cells, the difference in the cytotoxicity of Aβ(1-42) oligomers could be clearly distinguished. In addition, Aβ(1-42) oligomers induced mitochondria dysfunction, reactive oxygen species (ROS) generation, and calcium influx PrPC-dependently. Apoptosis, related to mitochondria dysfunction, was further investigated to determine the cytotoxic pathway; the results suggest that PrPC could be involved in both the intrinsic and extrinsic apoptotic pathways. Finally, cells with abundant PrPC expression seemed to be more susceptible to Aβ(1-42) oligomer toxicity, suggesting the importance of the level of PrPC expression in the induction of apoptosis.</P>

ACTN-3 유전자 다형성과 무산소성 파워 능력

김철현 ( Chul Hyun Kim ),이영익 ( Young Ik Lee ),김윤만 ( Yoon Man Kim ),조인호 ( In Ho Cho ),제임스전 ( James Jeon ),김지연 ( Ji Yeon Kim ),박재현 ( Jae Hyun Park ),김혜진 ( Hea Jin Kim ),신경아 ( Kyung A Shin ),이승주 ( Seong Ju 한국운동영양학회 2004 Physical Activity and Nutrition (Phys Act Nutr) Vol.8 No.3

The α-actinin is an actin-binding protein belonging to the spectrin protein superfamily. a-actinin-3(ACTN-3) expression is limited to skeletal muscle, especially Type Ⅱ muscle fiber. This gene has homozygosity for a premature stop codon resulting in a-actinin-3 deficiency. The deficiency in the type Ⅱ muscle is able to be compensated by a-actinin-2(ACTN-2). While that deficiency does not induce a disease phenotype, the ACTN-3 is highly conserved in evolutionary terms because of its functions independent of the ACTN2. Researchers have suggested that this trait is related to muscle function at the extremes of power performance. Therefore, we compared the relative frequencies of the ACTN-3 R577X polymorphism between anaerobic power athletes and control group. For this study, we recruited 158 sprint or power elite athletes and 414 healthy adults. The results of the current study showed significant differences in the genotype frequencies such that elite anaerobic power athletes have 4% higher of the RR genotype and 9% lower of the XX genotype than the healthy adults. With respect to the allele frequencies, the athletic group had significantly higher R allele frequency and significantly lower X allele frequency than the control group. In summary, these results suggest that the ACTN-3 R577X genotype may represent a genetic marker for anaerobic power performance.

Molecular Cloning of a cDNA Encoding a Ribulose - 1 , 5 - Bisphosphate Carboxylase Small Subunit in soybean

Tae Ju Cho,Kee A Chung,Kwang Joon Lee,Un Seong Chung Nam Jeong Cho,Quae Chae 생화학분자생물학회 1992 BMB Reports Vol.25 No.7

Light enhances the expression of soybean rbcS genes, which encode small subunits of ribulose-1,5-bisphosphate carboxylase/oxygenases. To isolate soybean rbcS genes, a soybean λ cDNA library was constructed with mRNA from light-grown soybean seedlings. The cDNA library was screened using a pea rbcS clone as a probe, and several rbcS ≤DNA clones were isolated. The largest insert DNA from the rbcS λ cDNA clones was subcloned into pBluescribe+, and designated pGSS8. Nucleotide sequence analysis showed that the pGSS8 has a full-length cDNA insert containing 31 nucleotides of 5` untranslated region. The pGSS8 contains an open reading frame of 178 amino acids, including a transit peptide of 55 amino acids. When RNA samples from various soybean tissues were prepared and subjected to Northern blot hybridization, it was shown that the soybean rbcS genes are expressed in green tissues, particularly in leaves.

교차소뇌해리현상을 보이는 환자는 새로운 운동학습이 가능 한가? : 단일사례연구

남희윤(Hee Yun Nam),이성아(Seong A Lee),임연주(Yeon Ju Lim),김원령(Won Ryeong Kim) 한국인지운동치료연구회 2011 한국신경인지재활치료학회지 Vol.3 No.1

Objective : This study is aimed at looking at how the new motor learning is going or not by comparing the patient showing the phenomenon of crossed cerebellar diaschisis with a normal person through darts-throwing. Methods : This study selected a 69-year-old man with the phenomenon of crossed cerebellar diaschisis and another 69-year-old man judged as neurologically normal as its study subjects. The process of this experiment consisted of Checking the distance from the bull s eye with a dot when two men playing darts. Results : As a result of the study, there appeared no significant difference between experimental conditions in the healthy man while there appeared a significant difference in experimental condition 1-2(p=.019), and 1-3(p=.019), but there appeared no significant difference in experiment 2-3. Looking at the results of the comparison between the healthy man and patient by experimental condition, there appeared a significant difference in all experimental condition. In the result of the analysis of variance between the three experimental conditions, there appeared no significant difference in the healthy man and the patient. Conclusion : Through a simple experiment, this study was able to discover that it s difficult for a patient with crossed cerebellar diaschisis to take motor learning requiring new movements.

Capsaicin induces apoptosis by generating reactive oxygen species and disrupting mitochondrial transmembrane potential in human colon cancer cell lines

Yang, Kyung Min,Pyo, Jong Ok,Kim, Gyu-Yeol,Yu, Rina,Han, In Seob,Ju, Seong A.,Kim, Won Ho,Kim, Byung-Sam SP Versita 2009 Cellular & molecular biology letters Vol.14 No.3

<P>Although genetic factors are a well-known cause of colorectal cancer, environmental factors contribute more to its development. Despite advances in the fields of surgery, radiotherapy and chemotherapy, the cure rates for colon cancer have not substantially improved over the past few decades. Capsaicin (trans-8-methyl-N-vanillyl-6-nonenamide), the principal pungent ingredient of hot chili pepper, has exhibited an anti-tumor effect in many cell types. However, the mechanisms responsible for the anti-tumor effect of capsaicin are not yet completely understood. In this study, we investigated whether capsaicin induces apoptosis in colon cancer cell lines. Capsaicin decreased cell viability in a dose-dependent manner in Colo320DM and LoVo cells. In addition, capsaicin produced cell morphology changes and DNA fragmentation, decreased the DNA contents, and induced phosphatidylserine translocation, which is a hallmark of apoptotic cell death. We showed that capsaicin-induced apoptosis is associated with an increase in ROS generation and a disruption of the mitochondrial transmenbrane potential. A possible mechanism of capsaicin-induced apoptosis is the activation of caspase 3, a major apoptosis-executing enzyme. Treatment with capsaicin induced a dramatic increase in caspase 3 activity, as assessed by the cleavage of Ac-DEVD-AMC, a fluorogenic substrate. In conclusion, our results clearly showed that capsaicin induced apoptosis in colon cancer cells. Although the actual mechanisms of capsaicin-induced apoptosis remain uncertain, it may be a beneficial agent for colon cancer treatment and chemoprevention.</P>

자궁경부 원추절제술과 관련된 자궁적출술에서 조직병리와 절단경계면의 침범여부에 기초한 잔류병소의 예측에 관한 연구

윤영민 ( Yun Yeong Min ),이주열 ( Lee Ju Yeol ),전현아 ( Jeon Hyeon A ),김홍배 ( Kim Hong Bae ),이근영 ( Lee Geun Yeong ),강성원 ( Kang Seong Won ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.5

목적 : 원추절제술 후 남은 자궁경부에서 잔류병소의 예측인자로 원추절제 경계면의 침범상태와 자궁경부 종양의 심도를 평가하고자 하였다. 연구 방법 : 1996년 1월부터 2002년 6월까지 원추절제술 후 자궁적출술을 시행 받은 146명의 환자들을 대상으로 후향적 연구를 시행하였다. 잔류병소의 예측인자로 환자의 나이와 출산력, 원추절제 검체의 조직병리정도와 절단면 침범상태가 분석되었다. 결과 : 절단면 양성의 이환율 LSIL 0%, HSIL 15%, CC-Ⅰa1 43.3%, CC-Ⅰa2 85.7%였다. 원추절제술 후 자궁적출술 검체에서 잔류병소 발생율은 LSIL 0%, HSIL 23.3%, CC-Ⅰa1 35.5%, CC-Ⅰa2 100%였다. 잔류병소는 양성 절단면인 경우에서 음성 절단면인 경우에서보다 유의하게 높은 빈도를 보였다. 양성 절단면의 경우 잔류병소에 대한 양성 예측율은 LSIL 0%, HSIL 22.2%, CC-Ⅰa1 57.6%, CC-Ⅰa2 100%였다. 양성 절단면의 경우 8.3%에서만이 자궁적출술에서 더 진행된 병변을 보였다. 음성 절담녀의 경우 잔류병소에 대한 음성 예측율은 LSIL 100%, HSIL 70.5%, CC-Ⅰa1 81.4%, CC-Ⅰa2 0%였다. 결론 : 원추절제술 양성 절단면과 잔류병소의 발생율은 자궁경부 종양의 심도에 따라 증가한다. 양성절단면인 경우 음성 절단면인 경우보다 잔류병소와의 높은 상관관계를 보였으나 음성절단면이 잔류병소가 존재하지 않는다는 것을 확신하지는 못한다. 또한, 양성 절단면이 항상 잔류병소의 존재와 지속성을 지적하지는 않는다. 그러므로 원추절제술 후 자궁적출술은 양성 절단면을 가진 고등급 자궁경부 종양이나 자궁경부암인 경우, 동시에 다른 자궁의 병변이 있는 경우나 지속적 추적관찰이 불가능한 경우의 환자에게 고려되어져야 한다. Objective : The objective of this study is to evaluate predictive factors for residual lesion after conization in patients with cervical neoplasia. Methods : we performed retrospective study (between January 1996 and June 2002) and reviewed 146 patients who had undergone cervical conization followed by subsequent hysterectomy. The age and the parity of patients, the histopathology and the status of resection margins of cone specimens were analysed as predictive values of residual lesions. Results : The prevalence rate of positive cone margins were 0%, 15%, 43.4%, and 85.7% respectively in patients with low-grade squamous intraepithelial lesion (LSIL) (n=3), high-grade squamous intraepithelial lesion (HSIL) (n=60), Cervical cancer stage Ⅰa1 (CC-Ⅰa1) (n=76) and Cervical cancer stage Ⅰa2 (CC-Ⅰa2) (n=7). The prevalence rates of positive residual lesions in post-cone hysterectomy specimens were 0%, 23.3%, 35.5%, and 100% respectively in those with LSIL, HSIL, CC-Ⅰa1, and CC-Ⅰa2. Residual lesions were significantly more frequently found in patients with positive cone margin (56.3%) than in those with negative margin (21.4%) (P value=0.000026). Positive predictive values of margin status for the presence of residual lesions were 0% (LSIL), 22.2% (HSIL), 57.6% (CC-Ⅰa1) and 100% (CC-Ⅰa2). Negative predictive values of margin status for the absence of residual lesions were 100% (LSIL), 76.5% (HSIL), 81.4% (CC-Ⅰa1) and 0% (CC-Ⅰa2). Only 8.3% (4/48) of patients with positive cone margin had more advanced residual lesions. 9.2% (9/98) of patients with negative margins had invasive residual lesion. Conclusion : The prevalence rate of positive cone margin and residual lesion increased with high severity of cervical neoplasia. Patients with positive cone margin had significantly higher chances of having residual lesion than those with negative margin. Free cone margin does not ensure the absence of residual lesion in the remaining cervix. Positive cone margin does not invariably indicate the presence and persistence of more severe residual lesion. Subsequent hysterectomy may be reserved for the patients with invasive cone pathology, concomitant morbid uterine condition, or not reliable for continuous follow-up.