d-pinitol regulates Th1/Th2 balance via suppressing Th2 immune response in ovalbumin-induced asthma

Lee, J.S.,Lee, C.M.,Jeong, Y.I.,Jung, I.D.,Kim, B.H.,Seong, E.Y.,Kim, J.I.,Choi, I.W.,Chung, H.Y.,Park, Y.M. North-Holland Pub ; Elsevier Science Ltd 2007 FEBS letters Vol.581 No.1

d-pinitol has been demonstrated to exert insulin-like and anti-inflammatory activities. However, its anti-allergic effect in the Th1/Th2 immune response is poorly understood. Recently, it was shown that T-bet and GATA-3 are master Th1 and Th2 regulatory transcription factors. In this study, we have attempted to determine whether d-pinitol regulates Th1/Th2 cytokine production, T-bet and GATA-3 gene expression in OVA-induced asthma model mice. We also examined to ascertain whether d-pinitol could influence eosinophil peroxidase (EPO) activity. After being sensitized and challenged with ovalbumin (OVA) showed typical asthmatic reactions. These reactions included an increase in the number of eosinophils in bronchoalveolar lavage (BAL) fluid, an increase in inflammatory cell infiltration into the lung tissue around blood vessels and airways, airway luminal narrowing, and the development of airway hyper-responsiveness (AHR). The administration of d-pinitol before the last airway OVA challenge resulted in a significant inhibition of all asthmatic reactions. Accordingly, this study may provide evidence that d-pinitol plays a critical role in the amelioration of the pathogenetic process of asthma in mice. These findings provide new insight into the immunopharmacological role of d-pinitol in terms of its effects in a murine model of asthma, and also broaden current perspectives in our understanding of the immunopharmacological functions of d-pinitol.

Effects of Dietary Fat Types on Growth Performance, Pork Quality, and Gene Expression in Growing-finishing Pigs

Park, J.C.,Kim, S.C.,Lee, S.D.,Jang, H.C.,Kim, N.K.,Lee, S.H.,Jung, H.J.,Kim, I.C.,Seong, H.H.,Choi, Bong-Hwan Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.12

This study was performed to determine the effects of dietary fat sources, i.e., beef tallow, soybean oil, olive oil and coconut oil (each 3% in feed), on the growth performance, meat quality and gene expression in growing-finishing pigs. A total of 72 crossbred pigs (Landrace${\times}$Large White${\times}$Duroc) were used at $71{\pm}1$ kg body weight (about 130 d of age) in 24 pens ($320{\times}150$ cm) in a confined pig house (three pigs per pen) with six replicate pens per treatment. The growing diet was given for periods of $14{\pm}3$ d and the finishing diet was given for periods of $28{\pm}3$ d. The fat type had no significant effect either on growth performance or on chemical composition or on meat quality in growing-finishing pigs. Dietary fat type affected fatty acid composition, with higher levels of unsaturated fatty acids (UFAs) and monounsaturated fatty acids (MUFAs) in the olive oil group. Microarray analysis in the Longissimus dorsi identified 6 genes, related to insulin signaling pathway, that were differentially expressed among the different feed groups. Real time-PCR was conducted on the six genes in the longissimus dorsi muscle (LM). In particular, the genes encoding the protein kinase, cAMP-dependent, regulatory, type II, alpha (PRKAR2A) and the catalytic subunit of protein phosphatase 1, beta isoform (PPP1CB) showed the highest expression level in the olive oil group (respectively, p<0.05, p<0.001). The results of this study indicate that the type of dietary fat affects fatty acid composition and insulin signaling-related gene expression in the LM of pigs.

Effect of grain-boundary flux pinning in MgB₂ with columnar structure

Kim, D.H.,Hwang, T.J.,Cha, Y.J.,Seong, W.K.,Kang, W.N. North-Holland 2009 Physica. C, Superconductivity Vol.469 No.15

We studied the flux pinning properties by grain boundaries in MgB<SUB>2</SUB> films prepared by using a hybrid physical chemical vapor deposition method on the c-axis oriented sapphire substrates. All the films we report here had the columnar grains with the growth direction perpendicular to the substrates and the grain sizes in the range of a few hundred nanometers. At very low magnetic fields, no discernable grain-boundary (GB) pinning effect was observed in all measuring temperatures, but above those fields, the effect of GB flux pinning was observed as enhanced critical current densities (J<SUB>c</SUB>s) and reduced resistances when an external magnetic field (B) was aligned parallel to the c-axis. We interpret the B dependence of J<SUB>c</SUB> in the terms of flux line lattice shear inside the columnar grains activated by dislocations of Frank-Read source while the flux lines pinned by GB act as anchors for dislocations. Magnetic field dependence of flux pinning force density for B parallel to the c-axis was reasonably explained by the above model.

Potential application of antibody-mimicking peptides identified by phage display in immuno-magnetic separation of an antigen

Hien, T.B.D.,Maeng, J.H.,Lee, B.H.,Seong, G.H.,Choo, J.,Lee, E.K. Elsevier Science Publishers 2012 Journal of biotechnology Vol.161 No.3

Phage display was performed against human IgG (hIgG) through five rounds of 'biopanning'. Each round consisted of: (1) incubating a library of phage-displayed 12-mer peptides sequences on hIgG-coated magnetic beads, (2) washing the unbound phages, and (3) eluting the bound phages. The eluted phages were either amplified to enrich the pool of positive clones or subjected to the next round without amplification. Through ELISA, four clones (F9, D1, G5, and A10) showing specific binding affinity to hIgG were identified. Among these, F9 had the highest affinity (K<SUB>d</SUB>=6.2nM), only one order of magnitude lower than the native anti-hIgG antibody (0.66nM). Following the DNA sequences of the selected clones, four 12-mer peptides were chemically synthesized. Among them, D1 peptide showed the highest binding affinity to hIgG via SPR biosensor measurements. This peptide was conjugated to biofunctionalized magnetic beads, and its immuno-binding ability was compared with that of the native antibody immobilized to magnetic beads. The mol-to-mol binding efficacy of the peptide-coated magnetic beads was approximately 1000-fold lower than that of the antibody-coated magnetic beads. Our results suggest a feasibility of using antibody-mimicking peptides identified by phage display technique for immuno-magnetic separation of an antigen.

양파 재배 생력기계화 정식기계 개발

민영봉,문성동,하종규,문경규,김도완,박중춘,정한택,제성룡,우철노,강환규,신광식,김성환,성경현,한규정,서전규,하인종 경상대학교 시설원예연구소 1999 施設園藝硏究 Vol.6 No.-

Characterization of a renewable extracellular polysaccharide from defatted microalgae Dunaliella tertiolecta

Geun Goo, B.,Baek, G.,Jin Choi, D.,Il Park, Y.,Synytsya, A.,Bleha, R.,Ho Seong, D.,Lee, C.G.,Kweon Park, J. Elsevier Applied Science 2013 Bioresource technology Vol.129 No.-

Extracellular polysaccharide (EPS) was isolated from defatted micro-algae Dunaliela tertiolecta and defined as linear (1→4)-α-d-glucan based on monosaccharide composition, enzymatic and spectroscopic analyses. Optimization and characterization of acidic and enzymatic hydrolyses of EPS have been performed for its potential use as a renewable biorefinery material. The hydrolytic methods were improved to assess the effect of substrate specificity, reaction time, pH, ionic strength and temperature on efficiency of glucose production. EPS was effectively converted into glucose within one-step enzymatic or acidic hydrolysis under optimized conditions. Over 90% recovery of glucose was achieved for both hydrolytic approaches. High potential production of EPS and high yield conversion of this substrate to glucose may allow further exploration of microalga D. tertiolecta as a potential biomass producer for biotechnological and industrial exploitation of bioethanol.

工業敎育大學 敎育改善에 關한 硏究(Ⅱ)

孟琦錫,洪元杓,任洪彬,李廷玟,朴萬植,李載元,金八圭,權五憲,成世鎭,朴東徹,金太均,李定根 충남대학교 공업교육연구소 1981 論文集 Vol.4 No.1

Since the College of Industrial Education of Chungnam National University took charge of technical teacher preparation in 1977, much efforts have been concentrated on the activities for creating favorable educational environments in terms of curricula, pedagogic affairs, inner and outer facilities, etc. In this study some imposed barriers which are likely to hinder this college from achieving better education were identified and analysed. Considerably large scale of the student enrollment in comparison with the market demand is a prevailing issue which causes an adverse effect for clarification of the educational goal of the college and another problem is concerned with the student entry criteria which do not require the pre-experiences in the field. Under this circumstances, a guide line to improve the teacher education is recommended as follow; 1) The number of the student enrollment should be adjusted to meet the minimum range in the wave of the technical teacher demand projection and the shortage of teacher could be covered by the graduates from the shortterm courses in this college. 2) The current curriculum is accordingly recommended to be diversified taking into consideration the qualification of the student. The suggested consists of four-year regular course, one-year course, and six-month course and the short-term courses are provided to induce the experienced people from industries. 3) In order to have teachers adjust themselves to changing technology, education on sound and fundamental knowledges should be provided in the pre-service education course and education on advanced knowledges and applied theories deferred to the in-service training course. 4) In the four-year pre-service education, the general education and pedagogic subjects are to be drastically intensified from 18 credits and 22 credits up to 40 credits and 30 credits respectively.

Ahnak functions as a tumor suppressor via modulation of TGFβ/Smad signaling pathway

Lee, I H,Sohn, M,Lim, H J,Yoon, S,Oh, H,Shin, S,Shin, J H,Oh, S-H,Kim, J,Lee, D K,Noh, D Y,Bae, D S,Seong, J K,Bae, Y S Macmillan Publishers Limited 2014 Oncogene Vol.33 No.38

We provide detailed mechanisms of Ahnak-mediated potentiation of transforming growth factor β (TGFβ) signaling, which leads to a negative regulation of cell growth. We show that Smad3 interacts with Ahnak through MH2 domain and that Ahnak stimulates Smad3 localization into nucleus leading to potentiating TGFβ-induced transcriptional activity of R-Smad. Moreover, overexpression of Ahnak resulted in growth retardation and cell cycle arrest through downregulation of c-Myc and cyclin D1/D2. We describe results from analyses of Ahnak<SUP>−/−</SUP> mouse model expressing middle T antigen in a mammary gland-specific manner (MMTV<SUP>Tg/+</SUP>Ahnak<SUP>−/−</SUP>), which showed significantly progressed hyperplasia of mammary glands compared with MMTV<SUP>Tg/+</SUP>Ahnak<SUP>+/+</SUP>. Finally, we screened multiple human breast cancer tissues and showed that the expression of Ahnak in cancer tissues is lower than that in control tissues by 50%. Taken together, these data indicate that Ahnak mediates a negative regulation of cell growth and acts as novel tumor suppressor through potentiation of TGFβ signaling.

Powder Blasting을 이용한 유리소재의 연료전지용 개질기의 유로 채널 가공

박동삼,성은제,한진용 한국공작기계학회 2006 한국공작기계학회 추계학술대회논문집 Vol.2006 No.-

In this study, introduce about processing method of fluid passage channel in fuel processor by powder blasting. A processing meterial of the channel used LCD glass which thermal resistance and corrosion resistance are superior. For the comparison of heat transfer performance of methanol evaporator, numerical analysis using FLUENT code was carried out. Thereafter, processed fluid passage channel for fuel processor of LCD glass by powder blasting. After processing, analyzed about comparison of several channel forming cases channel depth, width and rib width.

키토산 발효사료의 첨가가 비육돈의 도체특성 및 육질에 미치는 영향

김종원,김종덕,성기승,강석남 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.3

처리구 당 돼지 10마리씩을 선발하여 비육후기에 0.2%, 0.5%의 키토산 발효사료를 첨가하여 6주간(43일) 급여한 사양시험 결과는 증체량에 있어서는 0.2% 처리구가 가장 높았으며 (p<0.05) 사료요구율에 있어서는 0.5% 처리구가 가장 낮게 나타났다. 0.2% 수준에서의 첨가는 사료의 효율을 증가시키고 증체량을 개선하였다. pH값은 등심의 경우에 있어서 대조구가 5.27로서 가장 낮았고 0.2%와 0.5% 처리구는 각각 5.47과 5.54를 나타내었으며 처리구간에 있어서 유의한 차이를 보여 주었다(p<0.05). 등심의 경우 L*값이 0.2%와 0.5%의 두 처리구에 있어서 대조구보다 유의하게(p<0.05) 낮게 나타났으며, 목심의 경우네는 0.5% 처리구가 다른 처리구보다 유의하게(p<0.05) 낮은 것으로 나타났다. 3가지 처리에 따른 등심부위의 지방산 조성을 보면 현저한 차이를 발견할 수는 없으나 키토산 발효사료 처리구에는 C12:0 이나 C16:0과 같은 포화지방산의 비율이 높아진 반면 탄소수가 18개의 불포화 지방산 함량은 다소 낮아 진 것을 알 수 있다(대조구 : 30.15%, 0.2% 처리구 : 26.03%, 0.5% 처리구 : 26.94%). 관능검사항목 중에서 다즙성의 경우에만 처리구간별로 유의한 차이를 나타내었으며, 0.5% 처리구가 가장 높은 것으로 나타났다(p<0.05). 또한 연도에 있어서도 0.5% 처리구가 가장 부드러운 것으로 나타났다. 삽겹살에 대해서는 다즙성, 풍미 그리고 기호성을 조사한 바, 풍미와 기호성 측면에서도 0.5% 처리구가 가장 높은 것으로 나타났다(p<0.05) This experiment was carried out to investigate the effect of fermented chitosan additive(FCA) as a probiotics in finishing pig. The levels of 0.2% and 0.5% of FCA powder was added to the regular feed. A total of 30 pigs were used to evaluate the growing performance. Those FCA supplemented feeds were supplied to the pigs at 17 weeks of age for 6 weeks. After slaughtering, 4 pigs of each treatment were used to compare the physico-chemical characteristics and sensory evaluation. Addition of 0.2% of FCA improved the average daily weight gain and feed efficiency. The pH of the FCA treated loin was slightly higher than control. The L^*, a^*, b^* values of the treatment groups were generally low compared to control group. So the effect of FCA to meat color was expression of purplish red color. Juiciness of the pork shoulder treated with 0.5% FCA was significantly(p<0.05) higher than the other groups. Flavor and overall preference scores for bacon treated with 0.5% of FCA were significantly(p<0.05) higher than the other groups.