김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

ATPase 활성도에 미치는 카드뮴의 영향

이우석,박정덕,정규철 중앙대학교 의과대학 의과학연구소 1989 中央醫大誌 Vol.14 No.1

Effects of cadmium on ATPase activities were studied in microsomal fractions obtained from liver, kidney and testis of rats. The total ATPase activities were 5.829 ± 0.141 (S.D.) μmol/mg protein/hr, 35.992 ± 1.304μmol/mg protein/hr and 46.046 ± 0.961 μmol/mg protein/hr in microsomes of normal liver, kidney and testis, respectively. The total activities of liver and kidney microsomes were inhibited by 34.4% and 39.60%, respectively, by addition of 2 mM ouabain but that of testis was not influenced by ouabain. The inhibition of microsomal ATPase activity by cadmium were dose-dependent in the range of concentrations from 0.05 mM to 3.0 mM. The concentrations of cadmium causing 50% inhibition (I_so) of ATPase activity were calculated by probit method. Cadmium concentrations causing 50% inhibition of total ATPase activity were found to be 0. 458 mM, 0.310 mM and 0.730 mM in liver, kidney and testis, respectively. Ouabain insensitive (Mg^++-) ATPase of liver, kidney and testis were inhibited 50% by Cd in concentrations of 1.460 mM, 0.970 mM, 0.310 mM and 0.730 mM in liver, kidney and testis, respectively. Ouabain insensitive (Mg^++- ) sitive to Cd and were inhibited 50% in microsomes of liver and kidney by Cd concentrations of 0.086 mM and 0.058 mM, respectively. Thus ouabain-sensitive (Na^+, K(^+)-) ATPasc was 17 times more susceptible to cadmium than ouabain-insensitive (Mg(^++)- ) ATPase.

蒐集 在來種 고추의 特性(1988年度)

金炳洙,李愚升,權寧石,孫銀鈴 慶北大學校 1989 論文集 Vol.48 No.-

Twenty three local cultivars of pepper(capsicum annuum L.) were planted in the Kyungpook National University Experiment farm and their growth and fruit characteristics were investigated. Seedings grown separately were tested for resistance to bacterial spot(Santhomonas campestris pv. vesicatoria) and Phytophthora blight(Phytophthora capsici) by artificial inoculation. KC209 was the earliest in flowering followed by KC218, KC203, KC223, KC202, KC201 and KC200. KC213, KC217, and KC204 were leading in fresh red fruit yield in a descending order among the rest excluding KC205 and KC207, open-pollinated fruits of which were removed and flower buds were wrapped before blooming to guarantee self-pollination. Fruits of Kc207 was the largest and Kc221 and KC217 were also bearing relatively large iruits. None of the cultivars tested was resistant to either bacterial spot or Phytophthora blight. Collection of local cultivars of pepper was continued in 1988 and 7 local cultivars were collected in Kyungpook, Cheonbuk, and Cheonnam provinces and their seed fruit characters were recorded.

316LN 스테인리스 강에서 질소에 의한 결정립 미세화가 고온 기계적 특성에 미치는 영향

김대환,이요섭,류우석 대한금속재료학회 2004 대한금속·재료학회지 Vol.42 No.7

Mechanical tests, such as tensile, fatigue and creep, of type 316L(N) stainless steel containing 0.04 and 0.10 wt% nitrogen content were conducted at 600℃ to investigate the grain size effect on the behaviors of mechanical properties since grain size was refined with the addition of nitrogen. Yield strength and fatigue life increased with the addition of nitrogen and the decrease of grain size. The creep rupture time was increased with the addition of nitrogen but decreased with increasing grain size. The change in minimum creep rate with grain size was consistent with the result predicted from Garofalo's equation. (Received November 17, 2004)

고압 커패시터의 고장 분석을 통한 신뢰도 예측

양석준,김진우,신승우,이희진,신승훈,유동수,장석원 한국비파괴검사학회 2001 한국비파괴검사학회지 Vol.21 No.6

본 논문은 고압 커패시터의 고장분석과 신뢰성 예측 결과를 다루고 있다. 부품의 수명과 고장률을 예측하기 위해서 두 가지 방법으로 고장 모드와 고장 메커니즘을 연구하였다. 에폭시 수지로 성형된 고압 커패시터가 절연내압 시험 하에서 저항이 제로로 되는 고장에 대하여, 근본원인 고장분석 체계를 효과적으로 수립함으로써 고장 메커니즘의 원인을 분석하였다. 특히 세라믹-에폭시 계면에서의 절연파괴 고장 현상이 강조되었으며, 본 연구에서 얻어진 결과의 타당성은 마그네트론에 장착된 고압 커패시터의 열사이클 시험 수행에 의한 가속시험 결과로부터 입증되었다. 시험 결과들은 결함이 있는 로트를 신속히 규명하고 B10수명을 결정하는데 유용하게 사용할 수 있다. 또한, 유전체의 절연파괴에 대해서 부하-강도 간섭모델을 이용하여 고장률을 예측하였다. This paper presents a result of failure analysis and reliability evaluation for high voltage ceramic capacitors. The failure modes and failure mechanisms were studied in two ways in order to estimate component life and failure rate. The causes of failure mechanisms for zero resistance phenomena under withstanding voltage test in high voltage ceramic capacitors molded by epoxy resin were studied y establishing an effective root cause failure analysis. Particular emphasis was placed on breakdown phenomena at the ceramic-epoxy interface. The validity of the results in this study was confirmed by the results of accelerated testing. Thermal cycling test for high voltage ceramic capacitor mounted on a magnetron were implemented. Delamination between ceramic and epoxy, which might cause electrical short in underlying circuitry, can occur during curing or thermal cycle. The results can be conveniently used to quickly identify defective lots, determine B10 life estimation each lot at the level of inspection, and detect major changes in the vendors processes. Also, thd condition for dielectric breakdown was investigated for the estimation of failure rate with load-strength interference model.

CD34+ 조혈 모세포 이식 2례

김정아,정현식,김원석,윤성수,이홍기,박찬형,박성규,김동욱,이종욱,한치화,민우성,김춘추,김동집 대한조혈모세포이식학회 1996 대한조혈모세포이식학회지 Vol.1 No.1

Background: In most solid tumors, the CD34 antigen has not been detected, so positive selection of CD34+ cells may reduce tumor cell contamination and the CD34+ cells are capable of reconstituting hematopoiesis. We tried CD34+ cell transplantation in two patients. Method: CD34+ cells from chemotherapy + G-CSF mobilized PBPCs or bone marrow were positively selected with an avidin-biotin immunoadsorption column (CEPRATE SC system). Case 1. One course of chemotherapy using cyclophosphamide(200㎎/㎡) and etoposide (4.2g/㎡), combined with G-CSF(5㎍/㎏) S.C. was used in a relapsed lymphoma patient. This patient responded to the induction chemotherapy. CD34+ cells from harvested bone marrow were selected by the CellPro immunoadsorption column. The total number of mononuclear cells loaded onto the CellPro was 2.4×10^(8)/㎏, with 1.1% CD34+ cells. After column separation, the total number of positively selected cells was 5.16×10^(6)/㎏. The number of CFU-GM was 76.8×10⁴/㎏. This patient was treated with melphalan (140㎎/㎡) and TBI (1200cGy) and the positively selected CD34+ cells were infused. The time to neutrophil recovery greater than 0.5×10^(9)/L was 19 days and the time to platelet recovery greater than 50×10^(9)/L was 21 days. Case 2. Two courses of mobilizing chemotherapy were given 4 weeks apart using taxol(210㎎/m2) and adriamycin(60㎎/m2), combined with G-CSF(5㎍/㎏) S.C. in a breast cancer patients with 7 axillary node metastasis. CD34+ cells from each single leukapheresis product were selected by the CellPro immunoadsorption column. In the first collection, the total number of nucleated cells was 4.4×10^(8)/kg, with 0.42% CD34+ cells. In the second collection, the total number of nucleated cell was 2.8× 10^(8)/㎏ with 0.43% CD34+ cells. After colum separation, the total numbers of collected cells were 4.0×106/kg and 4.8×10^(6)/kg, the total number of CD34+ cells were 1.2×10^(6)/㎏ and 0.82×10^(6)/㎏. Colonogenic assays of positively selected CD34+ cells gave rise to myeloid erythroid, and multilineage colonies, with a median of 190 CFU-GM, 190 BFU-E, and 164 CFU-GEMM per 1×10³ adsorbed cells, respectively. High-dose chemotherapy with cumulative doses of 40mg/㎡ mitoxantrone, 750mg/㎡ thioptepa, and 1000mg/㎡ carboplatin was administered. Positively selected CD34+ cells were rapidly infused 24 hours after the end of high-dose chemotherapy. The time to neutrophil recovery greater than 0.5×10^(9)/L was 16 days and the time to platelet recovery greater than 50×10^(9)/L was 20 days.

A Study of the Influence of Strain Path Change on the Microstructure and Mechanical Properties of Low Carbon Steel Using Plate and Bar Rolling

Lee, Young Seog,Choi, Sang Woo,Lee, Soo Chan 대한금속재료학회(대한금속학회) 2001 METALS AND MATERIALS International Vol.7 No.2

This paper presents an experimental study of the effect of strain path changes with reference to rolling. 'The fundamental idea considered is to utilize the characteristics of plate rolling and bar rolling. Plate rolling is characterized as monotonic compressive loading while bar rolling is characterized as cross compressive loading. A four-pass plate rolling and bar rolling experiment was designed so that the material experiences the same amount of strain at each pass during rolling. The rolling experiment was carried out at moderately high temperatures (450, 550 and 650℃). The microstructures and mechanical properties of a low carbon steel acquired from two types of rolling experiments were compared. The results revealed that the differences in strain path attributed to monotonic loading (plate rolling) and cross loading (bar rolling) significantly influenced microstuctures and mechanical properties.

Which is the most accurate parameter to estimate the pulmonary wedge pressure in critically ill patients?

Woo Hyung Bae,June Hong Kim,Jun Hyuk Oh,Dong Won Lee,Sung Gook Song,Jeong Su Kim,Hyeon Gook Lee,Woo Seog Ko,Yong Hyun Park,Sang Gwon Lee,Kook Jin Chun,Taek Jong Hong,Yung Woo Shin 대한내과학회 2004 대한내과학회 추계학술대회 Vol.2004 No.1

Epidemiological Investigation on Botulism of Cattle in Northern Gyeonggi-do between 2011 and 2012

Woo seog Jeong,Jeong Kyoung Bae,Woo Jin Jeon,Youn Ju Kim,Ha chung Yoon,Woo Seok Chang,Sung Chul Hwang,Jee Hye Kim,Yong Ju Lee,Myeong Soo Kim,Do Soon Kim,Yi Seok Joo,So Young Park,Oun Kyong Moon 대한수의학회 2012 대한수의학회 학술대회발표집 Vol.2012 No.-

Changes in health-related quality of life and quality of care among terminally ill cancer patients and survival prediction: Multicenter prospective cohort study

Lee, Myung Kyung,Lee, Woo Jin,Do, Young Rok,Lee, Keun Seok,Jung, Kyung Hae,Heo, Dae Seog,Kim, Sam Yong,Park, Sang Yoon,Jeong, Hyun Sik,Kang, Jung Hun,Kim, Si-Young,Park, Sook Ryun,Yun, Young Ho Cambridge University Press 2015 Palliative & supportive care Vol.13 No.4

<B>Abstract</B><B>Objective:</B><P>This study examined changes in health-related quality of life (HRQoL) and quality of care (QoC) as perceived by terminally ill cancer patients and a stratified set of HRQoL or QoC factors that are most likely to influence survival at the end of life (EoL).</P><B>Method:</B><P>We administered questionnaires to 619 consecutive patients immediately after they were diagnosed with terminal cancer by physicians at 11 university hospitals and at the National Cancer Center in Korea. Subjects were followed up over 161.2 person-years until their deaths. We measured HRQoL using the core 30-item European Organization for Research and Treatment of Cancer Quality of Life Questionnaire, and QoC using the Quality Care Questionnaire-End of Life (QCQ-EoL). We evaluated changes in HRQoL and QoC issues during the first three months after enrollment, performing sensitivity analysis by using data generated via four methods (complete case analysis, available case analysis, the last observation carried forward, and multiple imputation).</P><B>Results:</B><P>Emotional and cognitive functioning decreased significantly over time, while dyspnea, constipation, and pain increased significantly. Dignity-conserving care, care by healthcare professionals, family relationships, and QCQ-EoL total score decreased significantly. Global QoL, appetite loss, and Eastern Cooperative Oncology Group Performance Status (ECOG-PS) scores were significantly associated with survival.</P><B>Significance of results:</B><P>Future standardization of palliative care should be focused on assessment of these deteriorated types of quality. Accurate estimates of the length of life remaining for terminally ill cancer patients by such EoL-enhancing factors as global QoL, appetite loss, and ECOG-PS are needed to help patients experience a dignified and comfortable death.</P>