The Status of DGNSS & Experimental Test of DGPS in Korea

Ko,Kwang-sub,Chung,Se-mo 한국항해항만학회 1997 한국항해항만학회 학술대회논문집 Vol.1997 No.5

DGNSS(Differential GNSS) may be most feasible positioning system to users who need the precise positioning in the future. A number of countries have carried out research and test about DGNSS based on the marine radiobeacon for improving positioning accuracy. This paper describes the status of DGNSS in the world and the system characteristics. In special, DGNSS network of Korea to be constructed is discussed. And then DGNSS, which are operating for test, is analyzed by an experimental approach.

초등학생의 과학에 대한 인지모니터링 기능의 효율성 분석

고광병,고세환,김범기,최관순 한국과학교육학회 2004 한국과학교육학회지 Vol.24 No.6

본 연구는 초등학생의 과학에 대한 인지모니터링 기능이 어느 정도인지, 또한 학년별 수준은 어떠한지를 알아보는데 그 목적이 있다. 연구 대상은 대전시에 위치하는 5개 학교에서 2~6학년까지 각 1개반을 표집하여 총 1001명을 대상으로 2~4학년용인 과학 인지모니터링 검사지Ⅰ(TSCMⅠ)와, 4~6학년용인 과학 인지모니터링 검사지Ⅱ(TSCMⅡ)를 개발하여 검사를 실시하였다. 학년별, 성별 과학 인지모니터링 기능의 효율성에 차이가 있는지를 알아보기 위해 분산 분석과 Scheffe´검증을 실시하였다. 연구 결과에 의하면 2~4학년의 인지모니터링 기능은 63.4%, 4~6학년에서는 57.4%의 효율성을 갖는 것으로 나타났다. 인지모니터링 기능의 효율성을 학년별로 구분 할 때, 3, 4학년은 2학년보다 유의미하게 높게 나타났으며, 5, 6학년이 4학년보다 더 높게 나타났다. 성별로 보면 2, 3학년은 여학생이 남학생보다 높았으며, 4~6학년은 성별 차이가 나타나지 않았다. The purpose of this study was to investigate the effectiveness of cognitive monitoring skills of elementary students on science by the grade and sex. The subject of this study was 1001 elementary students of 2~6 grade from 5 schools. The instrument of study, TSCM Ⅰ(Test of Science Cognitive Monitoring Ⅰ) for 2~4 grade and TSCM Ⅱ for 4~6 grade were developed. The results of this study showed that the effectiveness of cognitive monitoring skills was 63.4% for 2~4 grade and 57.4% for 4~6 grade. The effectiveness of cognitive monitoring skills of 3 and 4 graders was significantly higher than 2, while 5 and 6 was higher than 4 graders. Girls were higher than boys for 2 and 3 grade, but 4?6 graders were not significantly different by the sex.

임신부 뇨로부터 정제된 인간 상피세포 증식 인자 유사체의 in vitro bioassay 및 특성

박세철,전재현,남정현,권태종,고인영,유광현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

벤조산흡착, 음이온 교환수지, 단일클론항체을 이용한 immunoaffinity chromatography를 통하여 임신부 뇨로부터 천연의 hEGF를 정제하였다. 정제된 hEGF는 μ Bonda C_18 column을 사용한 HPLC 분석을 통하여 4개의 fraction으로 분리가 가능하였으며 western blot과 double immunodiffusion 실험 결과, 각각의 fraction이 hEGF의 특성을 가진 유사체인 것을 알 수 있었다. 또한 hEGF 표준 물질과의 spiking 및 아미노산 분석 등을 통하여 두 번째 fraction이 nhEGF와 동일한 것으로 확인하였다. nhEGF 및 그 유사체의 생물학적 활성 비교를 위하여 NIH 3T3 세포주에서 5'-Brdu incorporation 측정을 위한 labelling 시간, 혈청 농도의 최적 조건을 결정하였다. NIH 3T3 세포주의 DNA 합성능은 0.2% FCS가 포함된 저혈청 배지에서 hEGF가 0.1~10 ng/ml 농도로 첨가하였을 때 증가하는 경향을 나타냈다. HPLC를 통하여 분리된 두번째 hEGF 유사체가 다른 유사체보다도 생물학적인 활성이 우수하였으며, rhEGF 표준물질과의 spiking 및 아미노산 서열 분석등을 통하여 nhEGF로 밝혀졌다. 임신부의 뇨의 hEGF 유사체 함량중 natural hEGF는 46%이었다. Natural human epidermal growth facto (nhEGF) was purified from pregnant human urine by benzoic acid adsorption, DEAE-Sepharose ion exchange, and immunoaffinity chromatography. The purified nhEGF was further separated into four fractions using Bondapak C_18 HPLC system. Following characterization by Western blot and double immunodiffusion, we found that each fraction corresponds to four derivatives of the nhEGF. For biological analysis of nhEGF, we optimized the labeling time and serum concentration for the incorporation of 5-bromo-2'-deoxy-uridine (BrdU), a non-radioactive alternative for [^3H]-thymidine uptake, into NIH 3T3 cells. The DNA synthesis of NIH 3T3 cells was gradually increased at the nhEGF concentrations between 0.1~10 ng/ml in the Dulbecco's Modified Eagles Medium (DMEM) containing 0.2% Fetal calf serum (FCS). When we assayed the biological activity of four fractions, the activity of the second fraction was superior to that of the others.

각종 질소·인 제거공정에서 도출된 미생물 성장 동역학 계수 비교 분석

임세호,고광백,오영기 한국물환경학회 2004 한국물환경학회지 Vol.20 No.6

In this study, some of bacterial growth kinetic parameters were delineated and evaluated for the biological nutrient removal processes such as the A²/O, 4stage-BNR, Intermittent Cycle Extended Aeration System(ICEAS) and Intermittently Aerated Cylindrical Oxidation Ditch(IACOD) processes. Y_H values for the ICEAS process ranged from 0.71 to 0.74, and were higher than those for the other processes. It seems to indicated that organic carbons uptaked by microorganism were more used up for cell synthesis rather than for energy components in the ICEAS process. b_H for the ICEAS and IACOD processes were lower than those for A²/O and 4stage-BNR processes. The μ_(max·A) for the ICEAS was higher than those for the other processes, which indicated that desirable operating conditions for nitrifying bacteria's growth were established.

PelB Signal Sequence로 유도된 제조합 인간 상피세포 증식인자 분비 발현 벡터의 제조

박세철,남정현,김정근,권태종,고인영,유광현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.5

전사개시 단계중에 삽입되는 N말단의 methionine이 제거되고 pelB signal sequence를 삽입하여 인체 epidermal growth factor (hEGF)가 직접 분비발현 되도록 고안된 T7과 tac promoter에 의하여 각각 발현되는 pYHB101과 pYHB2를 제조하였다. 또한 T7 promoter에 의하여 발현되지만 signal sequence를 삽입하지 않은 pYHB1도 제조하여 각각 제조된 재조합 plasmid들의 발현 및 분비효율을 비교하였다. pelB signal sequence hEGF 유전자를 갖도록 제조된 pYHB101을 포함하고 있는 균주가 pYHB1보다 균체 성장속도는 낮았지만 pYHB101을 1 mM IPTG로 5시간 induction 하였을 때 13 mg/l의 rhEGF가 분비발현되어 T7 promoter의 발현 체계를 갖는 경우가 tac promoter보다 높은 발현 효율을 보였다. 이때 pYHB101에서 발현되는 rhEGF의 발현량은 준최적 조건에서 14 mg/l이었다. We have designed nucleotide sequences of hEGF structural gene to eliminate the N-terminal methionine residue incorporated during the translation initiation step, and constructed recombinant human epidermal growth factor (rhEGF) secretion plasmids pYHB101, and pYHB2 in which pelB signal sequence-hEGF gene was expressed under the control of the T7, and tac promoter, respectively. We also constructed pYHB1 vector which contains rhEGF gene controlled by T7 promoter. The transformant with pYHB101 showed relatively slow growth pattern compared to the transformant with pYHB1. However, we observed that the transformant with pYHB101 secreted rhEGF of 13 mg/l significantly after 5 hr induction with 1 mM IPTG and that the T7 promoter was more effective than tac promoter when connected to pelB signal sequence. The amount of rhEGF was 14 mg/l under the sub-optimized condition.

도심 교차로 노면 지표유출수의 초기수질특성에 관한 연구

최상용,고광백,이지영,임세호 한국환경과학회 2006 한국환경과학회지 Vol.15 No.4

극단 저체중 신생아에서의 동맥관 개존증 결찰술을 위한 진정맥 마취 경험 2례

최영균,고명진,이상은,조광래,김영환,임세훈,이정한,이근무,정순호,김영재,신치만 仁濟大學校 白病院 2010 仁濟醫學 Vol.31 No.-

Running title: Cases of anesthesia for extremely low birth weight infant. Extremely low birth weight infants (birth weight < 1000 g) are prone to various morbidities such as respiratory distress syndrome, intraventricular hemorrhage, periventricular leukomalacia, patent ductus arteriosus, necrotizing enterocolitis and retinopathy. To accomplish successful anesthetic management, many precautions must be continuously taken during the operation. First, inspired oxygen concentration should be adjusted to avoid oxygen toxicity. Second, body temperature must be maintained adequately. Third, hemodynamic parameters should be kept stable. We report 2 cases of successful anesthetic management for extremely low birth weight infant who underwent ligation of patent ductus arteriosus at the neonatal intensive care unit.

Methexymethyl sulfide를 이용한 합성연구

김정순,고영관,김대광,윤세중 공주대학교 기초과학연구소 1992 論文集 Vol.30 No.1

For the preparation of broad range of substituted aryl derivatives from the bromophenyl methoxymethyl sulfide and the bromobenzyl methoxymethyl sulfide, various functional groups such as alkyl, ester and haloketone could be easily introduced in the aromatic methoxymethyl sulfides by treatment with various electrophiles. Substituted methoxymethyl phenyl sulfides were converted to the arenesulfonyl chlorides with chlorine in water-dichloromethane at 5∼10℃. And substituted benzyl methoxymethyl sulfides were also converted to the arylmethanesulfonyl chlorides with N-chlorosuccinimide in aqueous acetic acid, at 5∼10℃.

A clinical and histopathologic study in alopecia associated with discoid lupus erythematosus of the scalp: a retrospective single-center study

( Se Kwang Park ),( You Jin Jung ),( Young Wook Ko ),( Dong Uk Cheon ),( Won Seon Koh ),( Jeong Eun Kim ),( Joo Yeon Ko ),( Young Suck Ro ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.2

Background: Discoid lupus erythematosus (DLE) of the scalp leads to transient or permanent hair loss causing significant psychological distress. However, a few clinicopathological studies of scalp DLE have been published. Objectives: This study was to investigate the clinical and histopathologic features of scalp DLE. Methods: We performed a retrospective study of 42 patients diagnosed as alopecia with DLE by scalp biopsy. We reviewed the clinical photos, biopsy specimens and clinical courses. Results: Forty patients had underlying SLE and 34 patients were female. Twenty-six patients got multiple lesions. Clinically, scale (90%), erythema (80%), scleroatrophy (73%) and hyperpigmentation (70%) were the common clinical features. Histopathologically, Epidermis showed hyperkeratosis (70%), epidermal atrophy (56%), basement membrane thickening (70%), liquefactive degeneration and pigmentary incontinence (42%). All patients revealed various degrees of infiltration and 70% patients showed perifollicular infiltration (mid portion 83%). Perifollicular fibrosis was observed in 29 patients and dermal mucin deposit in 31 patients. Twenty-three patients (55%) showed positive hair regrowth. Conclusion: Our study defines the clinical and histopathologic features of scalp DLE with hair loss and showed hair regrowth in more than half of patients. We suggest active treatment is needed for those patients although some patients may have permanent hair loss.

거주 성능 관리 시스템을 활용한 평가 사례 연구

고세광(Ko, Se-Kwang),이희원(Lee, Hee-Won) 한국산학기술학회 2008 한국산학기술학회 학술대회 Vol.- No.-

본 논문은 거주 성능 관리 시스템을 이용하여 수원시 인계동 반경 1km지역에 대한 거주 성능 평가 적용 사례이다. 수치지도가 가지고 있는 기본 데이터를 EasyMap을 이용하여 1차, 2차의 단계로 가공 한 후 Geomania를 통해 데이터베이스를 구축한다. 구축된 데이터 베이스는 거주 성능 관리 시스템을 통해 분석/평가 자료로 사용된다.