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Lim, Min-Cheol,Park, Ji Young,Park, Kisang,Ok, Gyeongsik,Jang, Hyun-Joo,Choi, Sung-Wook Elsevier 2017 FOOD CONTROL Vol.73 No.2
<P>In this study, we report an approach for the automated separation and concentration of pathogenic bacteria from food samples as a pretreatment step before detection. We developed an automated immunomagnetic separation (IMS) system, which included pre-enrichment of food samples, incubation with immunomagnetic beads, and separation and concentration of immunomagnetic beads along with bound target bacteria using an automated instrument based on IMS technology. Magnetic recovery bars covered with glass cylinders were utilized for separation of magnetic beads in food samples introduced into collection tubes. The separated magnetic beads with bound bacteria were dispersed in 1 mL phosphate-buffered saline (PBS) in concentration tubes. The efficiency of separation and concentration of the target pathogenic bacteria by the fabricated system were determined using plate counting with E. coli O157:H7 and whole milk as the model target bacteria and real food matrix, respectively. To achieve high concentration and recovery yield, the spiked milk samples were enriched with buffered peptone water before incubation with immunomagnetic beads and introduction into the collection tubes. The entire procedure from pre-enrichment through to concentration was completed within 2 h with the automated IMS system. The proposed approach achieved over 100-fold concentration of inoculated E. coil O157:H7 from milk samples with over 20% recovery of viable cells after pre-enrichment. These results illustrated the feasibility of the developed auto-IMS platform for rapid inspection of food pathogens. (C) 2016 Elsevier Ltd. All rights reserved.</P>
Lim, Ki Moo,Lee, Jeong Sang,Gyeong, Min-Soo,Choi, Jae-Sung,Choi, Seong Wook,Shim, Eun Bo The Korean Academy of Medical Sciences 2013 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.28 No.1
<P>To quantify the reduction in workload during intra-aortic balloon pump (IABP) therapy, indirect parameters are used, such as the mean arterial pressure during diastole, product of heart rate and peak systolic pressure, and pressure-volume area. Therefore, we investigated the cardiac energy consumption during IABP therapy using a cardiac electromechanics model. We incorporated an IABP function into a previously developed electromechanical model of the ventricle with a lumped model of the circulatory system and investigated the cardiac energy consumption at different IABP inflation volumes. When the IABP was used at inflation level 5, the cardiac output and stroke volume increased 11%, the ejection fraction increased 21%, the stroke work decreased 1%, the mean arterial pressure increased 10%, and the ATP consumption decreased 12%. These results show that although the ATP consumption is decreased significantly, stroke work is decreased only slightly, which indicates that the IABP helps the failed ventricle to pump blood efficiently.</P>
Lim Yohwan,Kim Hye Jun,Yoon Sung Soo,Lee Sang Jun,Lee Myeong Hoon,Park Hyewon,Park Sun Jae,Jeong Seogsong,Han Hyun Wook 대한의학회 2023 Journal of Korean medical science Vol.38 No.23
Background: Exercise is an important method to control the progression of diabetes. Since diabetes compromises immune function and increases the risk of infectious diseases, we hypothesized that exercise may affect the risk of infection by its immunoprotective effects. However, population-based cohort studies regarding the association between exercise and the risk of infection are limited, especially regarding changes in exercise frequency. The aim of this study was to determine the association between the change in exercise frequency and the risk of infection among patients with newly diagnosed diabetes. Methods: Data of 10,023 patients with newly diagnosed diabetes were extracted from the Korean National Health Insurance Service-Health Screening Cohort. Self-reported questionnaires for moderate-to-vigorous physical activity (MVPA) were used to classify changes in exercise frequency between two consecutive two-year periods of health screenings (2009–2010 and 2011–2012). The association between changes in exercise frequency and the risk of infection was evaluated using multivariable Cox proportional-hazards regression. Results: Compared with engaging in ≥ 5 times of MVPA/week during both periods, a radical decrease in MVPA (from ≥ 5 times of MVPA/week to physical inactivity) was associated with a higher risk of pneumonia (adjusted hazard ratio [aHR], 1.60; 95% confidence interval [CI], 1.03–2.48) and upper respiratory tract infection (aHR, 1.15; 95% CI, 1.01–1.31). In addition, a reduction of MVPA from ≥ 5 to < 5 times of MVPA/week was associated with a higher risk of pneumonia (aHR, 1.52; 95% CI, 1.02–2.27), whereas the risk of upper respiratory tract infection was not higher. Conclusion: Among patients with newly diagnosed diabetes, a reduction in exercise frequency was related to an increase in the risk of pneumonia. For patients with diabetes, a modest level of physical activity may need to be maintained to reduce the risk of pneumonia.
The Role of Aquaporin-1 in Choroid Plexus After Systemic Hyponatremia
Lim, Sung Moon,Jung, Yong Wook 東國大學校醫學硏究所 2005 東國醫學 Vol.12 No.2
본 실험은 전신성저나트륨증 후 나타나는 뇌의 병리 생리학적 변화과정에서 맥락막얼기에 존재하는 aquaporin-1(AQP1) 단백질의 역할에 대하여 알아보기 위하여 시행되었다. 전신성저나트륨증은 30㎖(체중의 12%)의 dextrose용액과 1-deamino-8-D-argine vasopression(dAVP)을 복강과 피하조직에 동시에 투여하여 유발하였다. 투약 후 2시간군과 6시간군에서 혈청 내 오스몰농도와 나트륨의 농도는 각기 252±5.2, 252±6.7mOsm/L과 117±1.7, 97±2.0mM로 감소하였다. 전신성저나트륨증으로 나타나는 제3뇌실 맥락막얼기에서의 AQP1단백질의 변화는 면역조직화학법과 정량적 면역블로트검사법을 이용하여 관찰하였다. 전신성저나트륨증 유발 후 2시간군에서 AQP1의 발현은 대조군에 비하여 의미있게 증가하였으며(28.2%±2.7%, n=7, P<0.05) 6시간군에서는 2시간군에 비하여 약간 감소하였다(15.7%±2.2%, n=7, P<0.05). 또한 전신성저나트륨은 제3뇌실에 인접한 치아이랑세포들의 자연사를 초래하였으며, AQP1이 최대한 증가한 2시간군에서 TUNEL 염색된 수가 가장 많이 관찰되었으며 이 후 AQP1의 감소와 함께 사라지는 양상을 보였다. 이러한 결과들은 전신성저나트륨증 후 혈액-뇌척수액장벽에서의 AQP1을 매개로한 수분의 과도한 유입이 두개강뇌압을 증가시키고 이로 인해 치아이랑은 세포들의 자연사를 초래한 것으로 생각된다. 따라서 맥락막얼기에서의 QAP1은 전신성저나트륨증 후 나타나는 뇌세포의 병리학적 과정에 중요한 역할을 담당할 것으로 생각된다. The expression of aquaporin-1 (AQP1) protein, a water channel, in the choroid plexus epithelium was examined in the rat to determine the role of AQP1 in the pathophysiology of systemic hyponatremia. Systemic hyponatremia was produced by coadministration of 30 ㎖ hypotonic dextrose solution (140 mM) intraperitoneally and 3㎍ 1-deamino-8-D-arginine vasopressin subcutaneously. This hydration protocol produced severe systemic hyponatremia: Two and six hours after the drug administration. there were significant reductions in serum osmolarity (252±5.1 and 252±6.4 mOsm/L) and in Na^(+) concentration (117±1.7 and 97.2 mM) from the control values (296±5.2 mOsm/L. 140±4.7 mM). AQP 1 expression in the third ventricle choroid plexus epithelium was determined by using immunohistochemistry and quantitative immunoblot analysis. After 2 h of systemic hyponatremia AQP1 expression was significantly increased by 28.2± 2.7% (n=7. P<0.05). whereas AQP1 expression was attenuated to 15.7±2.2% (n=7, P<0.05) after 6 h of systemic hyponatremia. In addition, systemic hyponatremia produced apoptotic cell death of granule cells in the dentate gyrus adjacent to the third ventricle. TUNEL staining revealed apoptotic granule cells after 2 h of systemic hyponatremia. while few apoptotic granule cells were observed after 6 h of systemic hyponatremia. These findings suggest that AQP 1—mediated excessive water influx into the brain through the blood —cerebrospinal fluid barrier during systemic hyponatremia may elevate the intracranial pressure and result in the apoptotic brain cell death. Thus, AQP1 in the choroid plexus may play an important role in the pathogenesis of systemic hypontremia.
Effects of Replacing Sucrose with Various Sugar Alcohols on Quality Properties of Semi-dried Jerky
Sung Jin Jang,Hyun Wook Kim,Ko Eun Hwang,Dong Heon Song,Yong Jae Kim,Youn Kyung Ham,Yun Bin Lim,Tae Jun Jeong,Si Young Kim 한국축산식품학회 2015 한국축산식품학회지 Vol.35 No.5
The objective of this study was to investigate the effects of replacing sucrose with sugar alcohols (sorbitol, glycerol and xylitol) on the quality properties of semi-dried jerky. Total 7 treatments of jerkies were prepared as follows: control with sucrose, and treatments with 2.5 and 5.0% of sucrose replaced by each sugar alcohol, respectively. Drying yield, pH, water activity, moisture content, shear force, myofibrillar fragmentation index (MFI), 2-thiobarbituric acid reactive substance (TBARS) value, sugar content, and sensory evaluation were evaluated. Xylitol slightly decreased the pH when compared to the other sugar alcohols (p>0.05). The water activity of the semidried jerky was significantly reduced by treatment with glycerol and xylitol (p<0.05). The moisture content of semi-dried jerky containing various sugar alcohols was significantly higher than that of the control (p<0.05), while replacing sucrose with glycerol yielded the highest moisture content. The shear force of semi-dried jerky containing sugar alcohols was not significantly different for the sorbitol and glycerol treatments, but that replacing sucrose with 5.0% xylitol demonstrated the lowest shear force (p<0.05). The TBARS values of semi-dried jerkies with sugar alcohols were lower than the control (p<0.05). The sugar content of the semi-dried jerkies containing sorbitol and glycerol were lower than the control and xylitol treatment (p<0.05). In comparison with the control, the 5.0% xylitol treatment was found to be significantly different in the sensory evaluation (p<0.05). In conclusion, semi-dried jerky made by replacement with sugar alcohols improved the quality characteristics, while xylitol has applicability in manufacturing meat products.
Lim, Sung Nam,Seo, Jung Yoon,Jung, Dae Soo,Ahn, Wook,Song, Hoon Sub,Yeon, Sun-Hwa,Park, Seung Bin Elsevier 2015 JOURNAL OF ALLOYS AND COMPOUNDS Vol.623 No.-
<P><B>Abstract</B></P> <P>Spherical Li<SUB>1.167</SUB> <SUB>−</SUB> <I> <SUB>x</SUB> </I>Na<I> <SUB>x</SUB> </I>Ni<SUB>0.18</SUB>Mn<SUB>0.548</SUB>Co<SUB>0.105</SUB>O<SUB>2</SUB> (0⩽ <I>x</I> ⩽0.1) particles were prepared by spray pyrolysis, and subjected to electrochemical characterization for lithium battery applications. It was confirmed that Na doping enhances the charge/discharge rate capability. The structure of prepared samples was characterized by XRD: the <I>c</I>-axis lattice parameter increases with increase in the amount of Na ions (parameterized by <I>x</I>, above). The Na-doped sample with <I>x</I> =0.05 shows capacities of 208 and 184mAhg<SUP>−1</SUP> at high current densities of 1.0C and 2.0C, respectively. These values are enhanced, compared to values of 189 and 167mAhg<SUP>−1</SUP> for the bare sample. The ratio of the capacity at 1.0C to that at 0.1C is enhanced from 77% for the bare sample to 84% for the Na-doped sample with <I>x</I> =0.05. The Li diffusion coefficients obtained from the galvanostatic intermittent titration technique (GITT) are higher for Na-doped samples than for the bare sample. In particular, the Na-doped sample (<I>x</I> =0.05), in the potential range around 4V, has a higher <I>D</I> <SUB>Li+</SUB> value of 3.34×10<SUP>−9</SUP> cm<SUP>2</SUP> s<SUP>−1</SUP>, compared with 1.35×10<SUP>−9</SUP> cm<SUP>2</SUP> s<SUP>−1</SUP> for the bare sample. The Na-doped samples (0< <I>x</I> <0.075) show high capacity retention: the Na-doped sample (<I>x</I> =0.05) shows a capacity retention of 92% compared to 83% for the bare sample.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Spherical Na-doped Li-rich cathode material prepared by spray pyrolysis. </LI> <LI> Na-doped samples show better rate capability than that of bare sample. </LI> <LI> Na-doped sample has higher <I>D</I> <SUB>Li+</SUB> value at 4V compared with that of the bare sample. </LI> <LI> The cycle performance was enhanced from 83% to 92%. </LI> </UL> </P>