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Transmission ability of Zika virus with artificially infected Aedes albopictus in Korea
Yang Sung‐Chan,Lee Hee-Il,Kim Hyunwoo,Lee Wook‐Gyo 한국곤충학회 2021 Entomological Research Vol.51 No.8
Zika virus (ZIKV), a virus mainly occurring in South America, is now globally distributed. Understanding the pathogen transmission cycle in a vector is exceptionally important in developing disease control strategies. In this study, we performed ZIKV microinjection in Aedes albopictus (Skuese), to estimate its vertical and horizontal transmission ability. The virus infection rate was confirmed by real-time qPCR in the F1 generation derived from the ZIKV-injected Ae. albopictus. The minimum infection rate (MIR) of the F1 generation was highest when they were injected with the virus at 6–12 h (MIR: 16.1) and lowest at 12–24 h (MIR: 4.7) after feeding. In the developmental stage of the F1 progeny, MIR values were 1.0, 1.3, and 6.7 in each egg, larval, and adult stage, respectively, but ZIKV was not detected in the pupa stage. Virus transmission ability was not significantly different between the collection areas (Tongyeong and Jeju). Ae. albopictus demonstrated a high venereal transmission rate of ZIKV and was detected in males (6 pool/7 pool) and females (6 pool/7 pool), confirming that ZIKV can be transmitted from infected mosquitoes to uninfected mosquitoes via mating. This is the first study of the administration of a ZIKV microinjection to Ae. albopictus in Korea and suggests a possibility of a potential mechanism for the virus to survive during adverse conditions via vertical transmission.
강동수(Dong Soo Kang),최상용(Sang Yong Choi),이광찬(Kwang Chan Lee),김진승(Chin Seung Kim) 대한소화기학회 1993 대한소화기학회지 Vol.25 No.5
N/A The 19 months clinical experiences about 634 cases of laparoscpic surgery were reviewed at Sung-Ae General Hospital from July 1991 to Jan. 1993 The results were as follows; Among 168 cases of laparoscopic cholecystectomy and 5 cases of laparoscopic CBD exploration, we obtained better results with advantages of laparoscopic surgery including few complications, less morbidity and early return to work. In series of 361 cases of laparoscopic appendectomy, we obtained superior results especially in the cases of 1) uncertainty of clinically suspected appendicitis, 2) right lower quadrant pelvic pain without other pathology and 3) patients who wanted early return to work. In series of spontaneous pneumothorax with bullae and recurrent pneumothorax, the thoracoscopic bullectomy was much superior than open bullectomy. The laparoscopic Taylor procedures and laparoscopic bandlysis will be better choice in the management of intractable pain in duodenal ulcer and postoperative adhesion. The diagnostic laparoscopy will be much useful in the blunt and penetrating abdominal trauma.
Eun-Do Kim,Jin-Kwon Lee,Jin-Kyu Cho,Jae-Myung Kim,Ji-Ho Park,Ju-Yeon Kim,Sang-Ho Jeong,Young-Tae Ju,Chi-Young Jeong,Eun-Jung Jung,Young-Joon Lee1,Soon-Chan Hong,곽승진 대한종양외과학회 2019 Korean Journal of Clinical Oncology Vol.15 No.2
Purpose: Whether subtotal or total colectomy with primary anastomosis (PA) is safer than Hartmann procedure (HP) for left-sided colon cancer obstruction or perforation remains controversial. The purpose of this study was to compare postoperative morbidity, mortality, and defecation frequency between PA and HP for left-sided colon cancer obstruction or perforation. Methods: This retrospective study enrolled 54 patients from January 2014 to February 2018 who underwent emergency surgery due to left-sided colon cancer obstruction or perforation. Results: PA was carried out in 20 patients while HP was performed for 34 patients. Thirty-day mortality did not show significant difference between the two groups (15.0% vs. 14.7%, P=1.000). No anastomotic leakage occurred in PA group while three (8.8%) cases of stump leakage occurred in HP group. Stoma repair was performed for 13 cases (44.8%) and stoma reformation was performed for one case in HP group (7.7%). Stoma related complications occurred in five cases (17.24%). For patients after stoma repair, defecation frequency at 3 months after operation was 2.91±2.88 times per day in PA group and 2.86±2.63 times per day in HP group. At 1 year after operation, defecation frequency was changed to 1.40±1.12 times per day in PA group and 1.17±0.39 times per day in HP group. Conclusion: Primary ileosigmoid or ileorectal anastomosis for left-sided colon cancer obstruction or perforation is safe, and shows similar outcome of defecation frequency compared to H
Physical and chemical Effects on the sonication Treatment of chitosan solution
Lee, Keun Tai,Park, Seong Min,Park, Chan Kyu,Kim, Sang Moo 한국키틴키토산학회 1997 한국키틴키토산학회지 Vol.2 No.2
물리적 방법을 이용한 키토산 올리고당의 제조에 관한 기초 자료를 제시하기 위하여 키토산용액을 20kHz의 초음파로 처리하였으며, 초음파 처리 효과에 미치는 처리 조건의 영향을 조사하였다. 키토산 용액의 고유점도는 초음파 처리 5분까지는 급속하게 감소하였고 그 이후로는 서서히 감소하였다. 키토산 용액의 부피가 작을수록 초음파 처리효과는 컸으며, 키토산 용액의 온도는 초음파 처리에 별다른 효과가 없는 것으로 나타났다. 5분간 초음파 처리 후의 점도는 용매의 종류에 따라 큰 차이가 없었으나, acetate buffer의 경우 초음파 처리효과가 가장 컸다. pH가 높을수록 초음파 처리효과는 크게 나타났으며, 이온강도와 염의 종류에 따른 효과는 나타나지 않았다. 이상의 결과에서, 용액의 부피는 10~20ml, 온도는 20~30℃, 용액의 pH는 4.5, 그리고 용매의 종류는 acetate buffer로 하는 것이 최적 초음파 처리조건으로 추정된다. As the first step of studies related to production of chitooligosaccharides by physical methods, chitosan solution were sonicated with 20kHz and various treatment effects were examined to present fundamental data of sonicated chitosan solution. Intrinsic viscosity of chitosan solution sharply decreased from 3.76dl/g to 2.90dl/g until 5 minutes of sonication and then slowly decreased. With low volume of chitosan solution, sonication was very effective and temperature of chitosan solution slightly affected the efficiency of sonication. In case of changing the solvent, no significant differences were observed on the effect of sonication, however, acetate buffer had highest sonication effect among various solvents. The sonication effect was increased as the increasement of the value of pH, on the contrary, ionic strength and type of counterions showed no effect on sonication, With these results, we assumed that optimal sonication treatment would be as follows, solution volume was 10 ~20ml, temperature range was 20~30℃ , pH value of solution was 4.5 and type of solvent was acetate buffer
Is There a Relationship Between Bone Quality and Hearing Level?
Lee, Seung Yeol,Hong, Hyung Sun,Yang, Seung-Chan,Kim, Kyu-Sung,Kim, Hyun Ji Lippincott Williams Wilkins 2018 Otology & Neurotology Vol.39 No.9
OBJECTIVES:: This study evaluated the correlation between skull bone attenuation on temporal bone computed tomography (CT) and bone mineral density (BMD) of the central skeleton on dual-energy x-ray absorptiometry (DXA) and the correlation between bone quality and hearing level. STUDY DESIGN:: Retrospective observational. SETTING:: Tertiary referral center. PATIENTS/INTERVENTIONS:: One hundred and one ears of 101 patients who underwent temporal bone CT and DXA with pure tone audiometry within 3 months of CT. MAIN OUTCOME MEASURES:: Bone quality was measured by both bone attenuation of the clivus and petrous apex by setting a circular region of interest and by the cortical thickness of the occipital bone on CT. Partial correlation analysis was used to assess the correlation between bone quality around the skull on CT and BMD in the central skeleton after adjusting for age and sex. Partial correlations between pure tone audiometry and CT/DXA results were sought. RESULTS:: There was no significant correlation between measurements on CT and BMD on DXA. The hearing level partially correlated with BMD on DXA. The average threshold of bone conduction showed a significant correlation with BMD at the femoral neck (correlation coefficient −0.241, p = 0.020) and trochanter (correlation coefficient −0.244, p = 0.018), but there was no significant correlation between the average threshold of bone conduction and BMD at the lumbar spine (p = 0.177–0.332). CONCLUSION:: We could not find a relationship between hearing loss and bone quality. Hearing levels can be affected by various factors, so further studies are needed to assess regional temporal bone quality.
Sequence Analysis of the Nuclear Matrix - Associated DNA Segments
Lee, Sang Eun,Park, Sang Dai,Lee, Chee Gun,Kim, Chan Kil 한국유전학회 1988 Genes & Genomics Vol.10 No.4
Nuclear matrix isolated from synchronized LP1-1 cells at G1/S boundary digested with DNase 1 to separate a nuclear-matrix(N.M)-associated DNA segments from the rest Through the cloning of the N.M-associated DNA segments (300-500 base pair in size) into a pUC18 vector, 800 clones (named as pMRs) were selected with X-Gal/IPTG and amphiciline. Putative clones harboring replication origin sequence were sequenced and characterized. We found that pMR15 and 16 act as a good template for origins of DNA replication in in vitro replication assay. Furthermore, pMR15 shows A/T richness and has a palindromic sequence. However, pMR62 shows G/C richness (65.7%) and has a homologous sequence to that of Adenovirus replication origin i.e. TGAPyGCC AGG.