8-hydroxyguanine이 전립선비대증 발생에 미치는 효과

이재원,이석영,이형래,이상철,김용태,김원재 충북대학교 의학연구소 2002 忠北醫大學術誌 Vol.12 No.2

연구목적: 전립선비대증은 노화현상에 의해 발생하는 질환으로서 임상적으로 주로 50세 이상에서 증상이 나타난다. 산소 라디칼은 DNA의 염기에 다양한 손상을 미칠 수 있다. 산소 라디칼에 의한 DNA의 변화에는 여러 가지 형태가 있으며 이러한 변화들 가운데서 guanine이 8-oxoguanine (8-hydroxyguanine, oh8Gua)으로의 수산화(hydroxylation) 반응은 가장 흔히 관찰되는 형태로서 노화 및 만성 퇴행성 질환, 기타 여러 질환의 중요한 병인으로 인식되고 있다. 세포에 유해한 영향을 주는 oh8Gua의 생성으로부터 유전자를 보호하는 수복효소 중 8-oxoguanine-DNA glycosylase (OGG1)가 주된 역할을 하는 것으로 알려져 있는데 이 효소를 생산하는 유전자인 hOGG1은 사람마다 유전자형이 다르다. 따라서 본 연구에서는 전립선비대증이 노화와 관련된 hOGG1 유전자형에 따라서 발생에 차이가 있는지를 조사하였다. 대상 및 방법: 충북대학교 병원에서 전립선비대증으로 진단 받고 입원하여 경요도전립선절제술을 받은 환자로 병리 조직학적으로 전립선비대증이 확인된 81명을 환자군으로 하였으며, 충청북도내 10개 군에서 50세 이상의 건강한 성인 남자 764명을 대상으로 실시한 전립선 무료검진에서 경직장 초음파 검사상 전립선 크기 20 gm 이하, 국제 전립선 증상 점수(IPSS) 7점 이하, 요속 10 ㎖/s 이상, 전립선 특이 항원(PSA) 4.0 ng/㎖ 이하인 81명을 대조군으로 설정하였다. 이들에서 채취한 혈액에서 genomic DNA를 얻은 후 PCR-SSCP (polymerase chain reaction-single stranded conformation polymorphism), 직접 염기서열분석(direct DNA sequencing) 및 RFLP (restriction fragment length polymorphism)를 통해서 두 군 사이의 hOGG1 유전자의 다형성을 분석하였다. 결과: 대조군에서 codon 326형은 Ser326Ser형이 12례(14.8%), Ser326Cys형이 51례(63.0%), Cys326Cys형이 18례(22.2%)였으며 전립선비대증 환자군에서는 Ser326Ser형이 15례(18.5%), Ser326Cys형이 34례(42.0%), Cys326Cys형이 32례(39.5%)로 두 군 사이에 통계적인 유의성이 관찰되었다(p=0.022). 또한 Cys326Cys형과 나머지(Ser326Ser, Ser326Cys)형으로 나누어 시행한 통계 분석에서도 대조군에서는 각각 18례(22.2%), 63례(77.8%)였으며 전립선비대증 환자군에서는 32례(39.5%), 49례(60.5%)로 통계적인 유의성이 관찰되었다(p=0.017, OR=2.286, 95% CI=1.149-4.546). 결론: hOGG1 유전자의 형태와 전립선비대증의 발생과는 밀접한 관련이 있었다. 즉 hOGG1 Cys326Cys형을 가진 사람에서 전립선비대증이 발생한 가능성이 높을 것으로 사료된다. Purpose: 8-oxoguanine DNA glycosylase (OSS1) rapairs DNA by removing 8-oxoguanine (oh8Gua), a highly mutagenic oxidative DNA adduct. Recently, the human gene for OGG1 (hOGG1) was cloned and several genotypes have been reported. However, the implications of such genotypes in benign prostatic lyperplasia have not been demonstrated. To assess the involvement of hOGG1 associated with the aging process on the developing of BPH, we analyzed the genetic polymorphisms of hOGG1. Materials and Methods: We studied the hOGG1 gene polymorphisms in 81 cases of BPH and 81 cases of normal controls. We performed the SSCP, PCR-based restriction fragment length polymorphism (RFLP) and direct sequencing to characterize the genetic polymorphisms of hOGG1 in both BPH patients and controls. Results: We found two polymorphic sites. A serine326/cysteine polymorphism at codon 326 (1a type) in exon 7 was associated with an exchange of amino acid. Another polymorphic site at codon 324 (1b type) in exon 6 was silent. We found that the distribution of hOGG1 genotypes in BPH patients (Ser326Ser type 18.5%, Ser326Cys type 42.0% and Cys326Cys type 39.5%) was significantly different from that in controls (14.8%, 63.0% and 22.2%, respectively) (p=0.022). Homozygosity for the Cys326Cys genotype significantly increased the risk of developing BPH, with the odds ratio (OR) being 2.286 (95% confidence interval [CI]=1.149-4.546) Conclusion: Our results suggest that the hOGG1 Cys326Cys genotype might play a important role in the development of BPH.

肥肉豚의 血液化學値에 關한 硏究

李政吉,康炳奎,魏聖河,朴承柱,朴來生 全南大學校 農漁村開發硏究所 1981 農業科學技術硏究 Vol.16 No.1

全羅南道 順天地方을 中心으로 한 養豚場에서 出荷된 肥肉豚을 對象으로 血液化學値를 調査했다. 돼지의 年齡은 7個月부터 13個月이었으며, 總 276頭(♀ 181,♂ 95)를 對象으로 屠畜場에서 採血했는데 Landrace와 Berkshire의 一代難種이 大部分이었다. 한편 本 實驗에서 使用한 무??豚은 모두가 去勢한 것이었다. 1. 測定한 血液化學値는 血淸?萄糖量, 血淸總蛋白量, ablumin量, globulin量, 血淸尿素?素量, 血淸 cholesterol量, calcium量, 無機燐量, 血淸 transaminase(SGOT와 SGPT)等이었다. 2. 本 調査에서 나타난 각종 血液化學値의 範圍 및 平均値는 이미 報告된 結果와 거의 一致하였다. 3. 血淸cholesterol量은 性別差가 없었고 겨울에 높은値를 보인 反面, 여름과 가을에는 낮은 치를 보였다. 4. 血淸總蛋白量의 季節的變動은 globulin量의 變動에 起因한 것이었고, globulin量은 全般的으로 높게 나타났는데 特히 여름철의 增加가 뚜렷했다. Samples of blood were taken from 276 fattening pigs(181 intact females and 95 castrated males) at slaughter and serum chemical values were determined. The pigs were from Suncheone area, Chonnam Province; their age ranged 7 to 13 months; most of them were crossbreds of Landrace and Berkshire. 1. Determined blood chemical values in the prestent study were glucose, total protein, albumin, globulin, serum urea nitrogen, cholesterol, calcuim, phosphorus, serum glutamin oxalacetic transaminase and serum glutamic pyruvic transaminase. 2. The ranges and means of the chemical values in the present study were reconcilable with the normal values previously reported. 3. Sex differences between castrated males and intact prepubertal females were not observed in serum cholesteol levels. There were significant differences between the means of seasons, with the higher cholesterol values being observed during the winter as compared with summer and fall. 4. Seasonal changes of total protein cantent were due to the seasonal changes of globulin values. The globulin values in the present study were higher than those reported previously by others; the values were highest during summer season.

급성 심근경색증 진단을 위한 Triage® Cardiac System의 진단적 유용성 : Comparison with Established Devices

이상래,김미란,노준영,류석용,김홍용,김성준 대한응급의학회 2002 대한응급의학회지 Vol.13 No.4

Purpose: The purpose of this study is to evaluate the diagnostic value of the Triage® Cardiac System (Biosite, USA) for quantitative detection of acute myocardial infarction. Methods: During a three-month period, we prospectively studied the cases of patients who visited the emergency department (ED) with suspicious acute ischemic chest pain. With the exclusion of patients with stable angina (according to ACC/AHA guideline) and non-cardiogenic chest pain, a total of 20 patients were studied. We evaluated the sensitivity and the specificity of the Triage® Cardiac System, and we compared the results with those from an established device (Elecsys® 1010, Boehringer Mannheim, Germany) by using a Pearson correlation and the student t-test. Results: The mean operating time of the Triage® Cardiac System was faster than that of the established device (16±1.2 min vs 30±0.6 min). The sensitivity and the specificity were same for the two devices. The sensitivity and the specificity of CK-MB were 63.6% and 88.9% respectively, and those of cardiac troponin were 54.5% and 100%. In the evaluation of patients who arrived at the ED after 4 hours of symptom onset, the sensitivity and the specificity were high. The Pearson correlation of CK-MB between the two devices was significant (r=0.970, p<0.01). The CK-MB values of acute myocardial infarction were significantly different with unstable angina (Triage® Cardiac System: t=2.857 p=0.017; Elecsys® 1010: t=2.804 p=0.018). Conclusion: The Triage® Cardiac System is a good diagnostic device for acute myocardial infarction, and its fast reporting of the result and its ease to use in the ED are superior to those of the established device.

Disulfiram-Alcohol Reaction(DAR)에 의한 저혈압 1예

이상래,류석용,김홍용 대한응급의학회 2002 대한응급의학회지 Vol.13 No.4

Disulfiram, or antabuse, is used in the treatment of chronic alcoholism since it causes an unpleasant aversive reaction to alcohol. It works by inactivating hepatic aldehyde dehydrogenase, leading to a pronounced rise in the acetaldehyde concentration when ethanol is metabolized. Acetaldehyde causes alcohol sensitivity, which involves vasodilation associated with increased skin temperature, subjective feelings of hotness and facial flushing, increased heart and respiration rates, lowered blood pressure, a dry-mouth or throat sensation associated with bronchoconstriction and allergy reactions, nausea, and headache. One of its metabolites, diethyldithiocarbamate (DDC) can inhibit the enzyme dopamine β-hydroxylase (DBH) through copper chelation. This may account for the profound refractory hypotension seen with the disulfiram-ethanol reaction (DAR), resulting from norepinephrine depletion. This report is presents the case of a patient we met, who presented with severe hypotension caused by the disulfiram-alcohol reaction, and along with a brief review of the subject.

연속공정시스템의 고속 고정도 장력제어시스템

이진섭,백형래,이상일 조선대학교 에너지.자원신기술연구소 2001 에너지·자원신기술연구소 논문지 Vol.23 No.1

This paper deals with the tension control of state feedback control for continuous processing line. At the starting point of entry section, for example, a unwinder named pay off reel unwinds the material maintaining constant tension and line speed. Speed and torque characteristics state is variance during acceleration and deceleration period. In simulation roll diameter variation and inertia variation are considered. It becomes clear that the proposed tension control system has high accuracy performance.

자궁경부종양에서 Cathepsin-D 표현의 의의

이춘희,김세진,전상식,이택후,조영래,채종민 경북대학교 병원 1997 경북대학교병원의학연구소논문집 Vol.1 No.1

Various clinical and histopathologic characteristics are currently used to obtain prognostic information about cervical carcinoma, but they do not predict the outcome for any individual patients. Thus, there is a need to identify additional tumor characteristics that are able to predict more accurately the outcome for an individual patient with cervical cancer. In this study, we explored the relationship between cathepsin-D expression and progression of the cervical neoplasia, the correlation between response to neoadjuvant chemotherapy and cathepsin-D expression, and we investigated if tumor cell cathepsin-D expression could serve as a prognostic factor in cervical carcinoma. Tumor tissues were obtained from 14 patients with cervical intraepithelial neoplasia and 52 patients with squamous cell carcinoma of the cervix. Cathepsin-S expression was identified by immunohistochemical methods using monoclonal antibody cathepsin-D (BioGene). Positive cathepsin-D immunoreaction in greater than 30% of carcinoma cells was scored as high expression. High cathepsin-D expression was seen in 15 of 52 invasive cancer but was absent in cervical intraepithelial neoplasia. It was shown that cathepsin-D expression was independent of the rumor grade, tumor size, lymph node involvement, depth of invasion, parametrial invasion, and response to chemotherapy. In disease free survival analysis by log-rank test, cathepsin-D expression was not significantly associated with survival. These results show that cathepsin-D expression is not a clinically useful adjunct to assessment of prognosis in invasive squamous cell carcinoma of the cervix.

만성 변비환자에서 발생한 자발성 후복막기종 1예

이상래,류석용,김홍용 대한응급의학회 2002 대한응급의학회지 Vol.13 No.4

The pneumoretroperitoneum has many etiologies. These range from the clinically insignificant to the potentially fatal, if not recognized promptly. Spontaneous pneumoretroperitoneum can develope from ulcerative colitis, colonic diverticulitis, or duodenal ulcer perforation. It can be associated with pneumothorax, pneumomediastinum, respiratory tract rupture, etc. An isolated pneumoretroperitoneum are a more obscure symptom than a pneumoperitoneum. Mostly, the diagnosis of retroperitoneal air depends of radiologic methods, such as simple X-ray, ultrasound (US), or computerized tomography (CT) measurements. In this case, we diagnosed a pneumoretroperitoneum from CT and found that if caused the perforation of the colon spontaneously, without any underlying diseases, due to chronic constipation without underlying diseases

국제전립선증상점수와 요류속도 및 전립선용적과의 상관성에 대한 고찰

이상철,이형래,김용태,김원 大韓泌尿器科學會 1998 Korean Journal of Urology Vol.39 No.4

비정질 Fe-B-Si-Al계 합금의 자기적 성질에 관한 연구

이상래 釜山大學校生産技術硏究所 1999 生産技術硏究所論文集 Vol.56 No.-

급냉응고된 Fe81-xB13Si6Alx(0≤x≤10)합금의 비정질 형성능, 열적 성질 및 자기적 성질이 조사되었다. Fe 대신에 Al을 치환하면 비정질상의 결정화시 첫 번째 결정피이크와 두 번째 결정피이크의 온도간격이 증가하였는데, 이것은 다음의 열처리에 의해서 나노결정질에 비정질 합금의 제조가능성을 시사한다. 모든 합금조성에서 가장 좋은 자기적 성질을 625K에서 1시간동안안 시효함으로서 얻을 수 있었다. Al의 첨가는 보자력을 증가시키고 자속밀도는 가소시켰다. 모든 조성에서 철손은 거의 비슷하고 시효온도의 증가와 함께 급격하게 감소하였다. Amorphous Fe81-xB13Si6Alx(0≤x≤10) alloys have been formed and their thermal stability and magnetic properties have also been investigated. Substitution of Al for Fe is found to increase the difference of the 1st peak temperature for crystallization of smorphous phase(Tx1) and 2nd ones(Tx2), ΔTx(=Tx2-Tx1), indicating the possibility for the formation of nanocrystalline amorphous alloy. The best softmagnetic properties was obtained by aging at 625K for 1hr all the alloys. The addition of Al increased the coercivity. The core loss in all compositions is nearly similar and rapidly decreased with ageing temperatures.

負饋還 回路의 安定化에 關한 硏究

李相來 충주대학교 1979 한국교통대학교 논문집 Vol.12 No.2

A Feedback control system has many Advan tages than open Loop system. The system is unstable if a tranient disturbance persists indefinitely or increases until it is limited only by some nonl ineariity in the circuit. This paper design the three order system increased stability by delayed compensated alement in the open loop transfer funetion.