A novel role for bone-derived cells in ankylosing spondylitis: Focus on IL-23

Jo, Sungsin,Koo, Bon San,Lee, Bitnara,Kwon, Eunji,Lee, Young Lim,Chung, Heekyoung,Sung, Il-Hoon,Park, Ye-Soo,Kim, Tae-Hwan Elsevier 2017 Biochemical and biophysical research communication Vol. No.

<P><B>Abstract</B></P> <P>The main aim of this study are to explore the role of bone-derived cells (BdCs) in ankylosing spondylitis (AS) and determine the underlying molecular mechanisms of IL-23 production. Primary BdCs were isolated from diced bone of facet joints obtained during surgery from seven AS patients and seven disease control (Ct) patients. Osteoblastic activity of BdCs was assessed by measuring their alkaline phosphatase activity and by alizarin red staining. Osteoblast and endoplasmic reticulum (ER) stress-related genes were assessed by quantitative PCR, immunoblotting, immunofluorescence, and immunohistochemistry. In addition, expression of IL-23 in response to BIX (selective BIP inducer X)-induced ER stress was evaluated by qPCR and ELISA. Protein interaction and binding to IL-23 promoter were confirmed by Immunoprecipitation and Chromatin immunoprecipitation, respectively. Transcript levels of genes involved in osteoblast function, as well as of the ER stress marker were higher in the AS group than the Ct group, and elevated RUNX2, BiP and IL-23 expression were observed in the BdCs, serum, and bone biopsies from the AS group. BIX-induced ER stress stimulated osteoblastic activity and IL-23 secretion by upregulating RUNX2 expression. Furthermore, in AS BdCs, RUNX2 interacted with C/EBPβ to bind to IL-23 promoter and RUNX2 knockdown suppressed IL-23 secretion. These finding may provide a molecular mechanism involved in sustained ER stress in AS BdCs stimulates the activation of RUNX2 and C/EBPβ genes, leading to IL-23 production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Bones and its-derived cells from patients with AS showed an increase in ER stress. </LI> <LI> IL-23 cytokine was significantly higher in AS patients than in healthy controls. </LI> <LI> Inducing ER stress in AS exhibited an increase of bone-related genes. </LI> <LI> Inducing ER stress in AS was accompanied with augmentation of IL-23 cytokine. </LI> <LI> ER stress-induced RUNX2 is involved in IL-23 secretion and bone-related genes. </LI> </UL> </P>

Sodium Trans-Bis(methyliminodiacetato)Chromate(Ⅲ)의 두가지 다른 空間群 P1￣와 A2/n間의 相互 變換

李珍昊,宋柱昊,崔成山,郭相姬,吳美蘭,박성진,李揆旺,徐廷善,徐日煥 충남대학교 1996 忠南科學硏究誌 Vol.23 No.1

The structure of sodium trans-bis(methyliminodiacetato) chromate(Ⅲ), C_10H_14O_8N_2CrNa, has been elucidated with two space groups P 1-; a=8.9856(6), b=13.308(1), c=6.261(1)Å, α=76.38(1), β=110.361(9), r=107.394(6)˚, V=662(2)Å^3, Z=2, μ=0.47mn^(-1), d=1.831Mg/㎥, R=0.0295 for 1901 independent reflections with F^2>2σ(F^2), and A2/n; a=13.65(5), b=6.261(1), c=16.85(6)Å, β=113.05(1)˚, V=1324(7)Å^3, Z=4, R=0.0287 for 1013 independent reflections with F^2>2σ(F^2). There are two different asymmetric half molecules in triclinic system and one asymmetric half molecule in monoclinic system. The structures of three different molecules are very similar in conformations. The four carboxyl O atoms and the two imino N atoms in each molecule produce a distorted octahedral coordination geometry around the Cr atom. The average distances of Cr-O(carboxyl) and Cr-N(imino) are 1.956 and 2.061Å respectively. Molecules are held together by ionic forces. In this paper, it has been also shown that the cell constants, Miller indices, atomic coordinates, lattice directions and reciprocal lattices of the two space groups can be mutually transformed.

原點選擇에 따른 構造因子의 位相變化 : Triclinic, Monoclinic, Orthorhombic System에서 Primitive이면서 Centrosymmetric인 Space Groups에만 適用 Only for Primitive and Centrosymmetric Space Groups of Triclinic, Monoclinic and Orthorhombic Systems

徐日煥,李珍昊,郭相姬,崔成山 충남대학교 기초과학연구소 1995 忠南科學硏究誌 Vol.22 No.2

Triclinic, monoclinic, orthorhombic systems에서 primitive이면서 centrosymmetric space group이면 8가지 對稱中心點中 任意의 한 點을 原點으로 指定하기 爲하여 3個의 structure factor에게 任意의 phase를 割當할 수 있음을 보였다. 이들 phase들은 direct method에서 structure factor의 phase를 決定해가는 basic set이 될 수 있다. In any primitive and centrosymmetric space group in the triclinic, monoclinic, or orthorhombic systems, arbitrary signs can be allocated to three reflections in order to specify the origin at one of the eight centers of symmetry. These signs form a basic set, from which more and more signed reflections emerge in the process of direct method.

포르피린증 환자에서 보인 가역적 자기공명영상소견 1례

강석윤,마효일,곽규환,이상암 대한신경과학회 1998 대한신경과학회지 Vol.16 No.2

우수방사성의약품 생산시설 개발

신병철,정원명,박상현,이규일,박경배,박진호 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.2

Manufacturing facilities of the pharmaceuticals must meet certain level of the cleanness required so that foreign substances such as dust, moisture, heat, microorganism, or virus do not contaminate the product. In case of radiopharmaceuticals for medical treatment and diagnosis, not only should the operators and environment be protected from radiation but also need to be isolated from the foreign contaminant. Therefore, manufacturing facilities for radiopharmaceuticals must satisfy the design standards of both hot cell and clean room which are specified by GNP. However, standards of maintaining negative pressure for preventing spread of radioactive contaminant in isolated facilities conflict with the standards of maintaining positive pressure for keeping cleanness. To solve this problem, are pressure of hot cell was designed lower than in the adjacent area to meet standards of the radiation safety. To keep higher cleanness in certain part of the hot cell for filling, minimal relative positive pressure allows. In order to effectively maintain the cleanness that is required for production of Tc-99m generator, which takes 70% of whole demand of radiopharmaceuticals, the rooms placed in each side of production room are used as a buffer area and three lead hot cells are installed in production room. In this research, we established the appropriate engineered design concept for Tc-99m generator manufacturing facility, which satisfies both GMP cleanness standard for preventing particles, bacteria, other contaminants and the regulations of radiation safety for supervising and controlling the amount of radiation exposure and exhausted radioactivity. And the concept of multi-buffer zones is introduced to apply negative air pressure for hot cell with first priority and to continue relative positive air pressure for clean room.

췌관내 회충 미입증으로 발생된 급성 췌장염 1예

이영창,이태영,김홍수,이문호,박상흠,최우봉,정일권,성재락,황산,이낙원,목지오,김선주 대한소화기내시경학회 1997 Clinical Endoscopy Vol.17 No.5

Acute pancreatitis due to impaction of Ascaris lumbricoides into the pancreatic duct is very rare. Authors hnve experienced one case of pancreatic ascariasis. Patient was a 62-year-old female with epigastric pain. Abdominal USG and endoscopic retrograde cholangiopancreatography showed characteristic finding of pancreatic ascariasis. Ascaris was removed with palypectomy snare and acute pancreatitis was resolved completely.

J2.5dPathway: A 2.5D Visualization Tool to Display Selected Nodes in Biological Pathways, in Parallel Planes

Ham, Sung-Il,Song, Eun-Ha,Yang, San-Duk,Thong, Chin-Ting,Rhie, Arang,Galbadrakh, Bulgan,Lee, Kyung-Eun,Park, Hyun-Seok,Lee, San-Ho Korea Genome Organization 2009 Genomics & informatics Vol.7 No.3

The characteristics of metabolic pathways make them particularly amenable to layered graph drawing methods. This paper presents a visual Java-based tool for drawing and annotating biological pathways in two- and a-half dimensions (2.5D) as an alternative to three-dimensional (3D) visualizations. Such visualization allows user to display different groups of clustered nodes, in different parallel planes, and to see a detailed view of a group of objects in focus and its place in the context of the whole system. This tool is an extended version of J2dPathway.

유한요소해석을 이용한 고무차륜 주행로 진동 저감 구조 개발

김재식(Jae-Sik Kim),이호용(Ho-Yong Lee),이일화(Il-Wha Lee),홍희록(Hee-Rok Hong),이강산(Kang-San Lee),이다솜(Da-Som Lee),서명원(Myung-Won Suh) 한국도시철도학회 2020 한국도시철도학회논문집 Vol.8 No.1

고무차륜 경전철은 저렴한 비용과 중규모의 수송능력을 갖춘 철도 시스템이다. 그러나 주거 밀집지역 주행 시 발생하는 소음에 대한 대책은 부족한 실정이다. 이에 따라 진동을 저감하기 위한 방진대책은 추가로 연구될 필요가 있다. 본 연구에서는 주행로의 진동 저감을 위한 구조를 선정하기 위해 유한요소해석을 활용하여 진동 특성 해석 기법 및 모델을 구축하고, 진동저감 효과가 우수한 주행로 구조를 제안한다. 주행로 구조는 기존 콘크리트 주행로, 단순 방진재 주행로 및 철판방진재 주행로가 있으며 실제 운행 조건을 고려한 구조 및 진동 해석을 수행한다. Rubber wheeled light rail vehicle is a railway system with low cost and mesoscale transport capacity. However, the research for vibration isolator way is needed because of vibration generated by running. In this study, We developed a vibration characteristic analysis method and model of a runway using finite element analysis. And then, We proposed the runway with excellent vibration isolator effect. There are the variable type of the runway structures including concrete runway, rubber and steel-rubber vibration isolator runway. The vibration analysis analyzed vibration acceleration results based on actual driving conditions. As a result, the steel-rubber vibration isolator runway model is decreased by 86%

Poster Session : PS 1315 ; Endocrinology : Invasive Adrenal Tuberculosis with Persistent 18f-Fdg Uptake Mimicking Malignancy

( Chang Woo Shim ),( Eun Kyung Lee ),( Bin Hwangbo ),( Ho Seok Chi ),( San Ho Lee ),( Hye In Lee ),( Do Il Choi ),( You Jin Lee ),( Seog Yun Park ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Context: Adrenal tuberculosis was one of most common disease causing primary adrenal insufficiency. We report a case showing invasive adrenal tuberculosis mimicking malignancy. Case presentation: A 52 year old man visited our clinic with generalized weakness and bilateral adrenal masses found by abdominal computed tomography(CT) scan. Adrenal masses appeared to invasive to adjacent organs (pancreas and liver), which was suspicious for malignancy. Sputum acid-fast bacilli(AFB) stains were negative, but aspirate obtained from bronchoalveolar lavage detected AFB positive tuberculosis. After performing rapid ACTH stimulation test with synthetic ACTH injection, a diagnosis of primary adrenal insufficiency was con. rmed; this was related with tuberculosis infection in both adrenal glands. After 9 months of anti-tuberculosis therapy, a chest CT revealed that multiple consolidation of lung was markedly decreased, suggesting remission of pulmonary tuberculosis. However, the hypermetabolic adrenal mass were remained in size and avidity. To exclude adrenal malignancy, ultrasonography-guided gun biopsy of adrenal mass indicated chronic in. ammation with necrosis and positive tuberculosis polymerase chain reaction. After 3 months of tissue AFB culture, no microorganisms were isolated. Conclusion: Adrenal tuberculosis can show aggressive features of invading adjacent organs in CT scan. In addition, the FDG uptake of adrenal tuberculosis can be persisted after remission of pulmonary tuberculosis.

Myelin oligodendrocyte glycoprotein antibody-associated disease manifesting as intractable fever managed by bromocriptine: a case report

Lee Jin San,이석윤,Oh Seong-il 대한신경집중치료학회 2023 대한신경집중치료학회지 Vol.16 No.1

Background: Demyelinating events expressed as abnormal thermoregulatory responses are rare, but intractable fever in myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD) is very rarely reported.Case Report: A 56-year-old woman presented with quadriparesis with acroparesthesia. During the admission, fever developed and persisted for 2 days despite the administration of high-dose antipyretics. Brain magnetic resonance imaging (MRI) showed hyperintense lesions involving the upper brainstem. A diagnosis of MOGAD was made according to the clinical characteristics and presence of seropositive MOG antibody. After administration of oral bromocriptine (2.5 mg/day), fever was slowly controlled for a few days.Conclusion: The present case explained that persistent fever in MOGAD could manifest as an uncommon manifestation. The lesion in the upper brainstem within the brain MRI could be thought of as a lesion anterior to the periaqueductal gray and the lesion at that site could be the cause of the patient's persistent fever with unknown origin.