Characterization of Lactobacillus acidophilus Isolated from Piglets and Chicken

Ahn, Y.T.,Lim, K.L.,Ryu, J.C.,Kang, D.K.,Ham, J.S.,Jang, Y.H.,Kim, H.U. Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.12

Lactic acid bacteria were isolated from piglets and chicken and characterized. Lactic acid bacteria showing resistance to low pH and bile, adhesion to intestinal epithelium cells, and the inhibition of Escherichia coli and Salmonella spp. were identified as Lactobacillus acidophilus. L. acidophilus PF01 survived for 2 h in MRS broth adjusted to pH 2. L. acidophilus CF07 was less resistant than L. acidophilus PF01 to pH 2, but survived at pH 2.5 for 2 h. Both of isolates were able to grow in MRS broth containing 0.3% (w/v) bile, with L. acidophilus CF07 being more tolerant to bile than L. acidophilus PF01. L. acidophilus PF01 and CF07 adhered specifically to the duodenal and jejunal epithelium cells of piglet, and the cecal and duodenal epithelium cells of chicken, respectively. Both of isolates did not adhere to the epithelium cells of the various animal intestines from which they were isolated. When L. acidophilus was cultured with E. coli and Salmonella spp. in MRS broth, MRS broth containing 2% skim milk powder or modified tryptic soy broth at $37^{\circ}C$, L. acidophilus PF01 and CF07 inhibited the growths of E. coli K88 and K99, and S. enteritidis and S. typhimurium, respectively. Both of isolates were found to possess the essential characteristics of probiotic lactic acid bacteria for piglet and chicken.

Phase II study of S-1 combined with oxaliplatin as therapy for patients with metastatic biliary tract cancer: influence of the <i>CYP2A6</i> polymorphism on pharmacokinetics and clinical activity

Kim, K-p,Jang, G,Hong, Y S,Lim, H-S,Bae, K-s,Kim, H-S,Lee, S S,Shin, J-G,Lee, J-L,Ryu, M-H,Chang, H-M,Kang, Y-K,Kim, T W Nature Publishing Group 2011 The British journal of cancer Vol.104 No.4

<P><B>Background:</B></P><P>Advanced biliary cancer is often treated with fluoropyrimidine-based chemotherapy. In this study, we evaluated the efficacy and tolerability of a combination of S-1, an oral fluoropyrimidine prodrug, and oxaliplatin in patients with metastatic biliary cancer.</P><P><B>Methods:</B></P><P>Patients with histologically confirmed metastatic biliary cancer and no history of radiotherapy or chemotherapy were enrolled. Oxaliplatin was administered intravenously (130 mg m<SUP>−2</SUP>), followed by 14-day administration of oral S-1 (40 mg m<SUP>−2</SUP> twice daily) with a subsequent 7-day rest period every 21 days. Pharmacokinetic analysis of S-1 was performed at cycle 1. Patients were genotyped for <I>CYP2A6</I> polymorphisms (<SUP>*</SUP>1, <SUP>*</SUP>4, <SUP>*</SUP>7, <SUP>*</SUP>9 or <SUP>*</SUP>10), and pharmacokinetic and clinical parameters compared according to the <I>CYP2A6</I> genotype.</P><P><B>Results:</B></P><P>In total, 49 patients were evaluated, who received a median of four cycles. The overall response rate was 24.5%. Median progression-free and overall survival was 3.7 and 8.7 months, respectively. The most common haematological grade 3 out of 4 toxicity was neutropenia (14%), while non-hematological grade 3 out of 4 toxicities included anorexia (14%), nausea (12%), asthenia (10%), vomiting (10%), and diarrhoea (4%). Biotransformation of S-1 (AUC<SUB>0−24 h</SUB> of 5-fluorouracil/AUC<SUB>0−24 h</SUB> of tegafur) was 1.85-fold higher for the <I>*1/*1</I> group than for the other groups (90% confidence interval 1.37–2.49). Diarrhoea (<I>P</I>=0.0740), neutropenia (<I>P</I>=0.396), and clinical efficacy (response rate, <I>P</I>=0.583; PFS, <I>P</I>=0.916) were not significantly associated with <I>CYP2A6</I> genotype, despite differences in 5-FU exposure.</P><P><B>Conclusion:</B></P><P>The combination of S-1 and oxaliplatin appears to be active and well tolerated in patients with metastatic biliary cancer, and thus is feasible as a therapeutic modality. <I>CYP2A6</I> genotypes are associated with differences in the biotransformation of S-1. However, the impact of the <I>CYP2A6</I> polymorphism on variations in clinical efficacy or toxicity requires further evaluation.</P>

Mapping the Posterior Ledge and Optic Foramen in Orbital Floor Blowout Fractures

Wong Yu Cong,Goh Doreen S.L.,Yoong Celine S.Y.,Ho Cowan,Cai Elijah Z.,Hing Angela,Lee Hanjing,Nallathamby Vigneswaran,Yap Yan L.,Lim Jane,Gangadhara Sundar,Lim Thiam C. 대한성형외과학회 2023 Archives of Plastic Surgery Vol.50 No.4

Background The posterior ledge (PL) is a vital structure that supports the implant posteriorly during orbital floor reconstruction. This study describes a technique for mapping the PL in relation to the infraorbital margin (IM) in patients with orbital floor blowout fractures. This study establishes the location of the optic foramen in relation to the PL. Methods Facial computed tomography (FCT) scans of 67 consecutive patients with isolated orbital floor blowout fractures were analyzed using Osirix. Planes of reference for orbital fractures, a standardized technique for performing measurements on FCT, was used. Viewed coronally, the orbit was divided into seven equal sagittal slices (L1 laterally to L7 medially) with reference to themidorbital plane. The distances of PL from IM and location of optic foramen were determined. Results The greatest distance to PL is found at L5 (median: 30.1mm, range: 13.5– 37.1mm). The median and ranges for each slice are as follows: L1 (median: 0.0mm, range: 0.0–19.9mm), L2 (median: 0.0mm, range: 0.0–21.5mm), L3 (median: 15.8mm, range: 0.0–31.7mm), L4 (median: 26.1mm, range: 0.0–34.0mm), L5 (median: 30.1mm, range: 13.5–37.1mm), L6 (median: 29.0mm, range: 0.0– 36.3mm), L7 (median: 20.8mm, range: 0.0–39.2mm). The median distance of the optic foramen from IM is 43.7mm (range: 37.0– 49.1) at L7.

전도성 고분자의 광 특성을 이용한 가스센서의 개발

임채근,이상문,이윤수,허용현,방영일,권오식,허증수,이덕동 경북대학교 센서기술연구소 2002 센서技術學術大會論文集 Vol.13 No.1

여대생의 흡연실태와 흡연, 자아존중감, 우울 간의 관계연구 : 서울시내 4년제 대학교 중심으로

강선아,김세은,김지민,이미현,이수연,이예리,이현정,임나래,정민선,최보영,이자형,정덕유,김현경 이화여자대학교 간호과학대학 2009 이화간호학회지 Vol.- No.43

This study was conducted to find out the actual state of smoking among female college students, their knowledge of harmfulness of smoking and their attitude about abstinence from smoking and to analyze their reasons for starting smoking. By doing so, this study aimed to provide the preliminary data for the educational program that prevents smoking and helps smokers to quit cigarettes. Also, this study was conducted to compare the self-esteem and the propensity to depression between female smokers and female non-smokers. Before carrying out the study, the study subjects were investigated about their gender, age, living allowance, residential condition, religion, high school, and whether or not they repeated a year in their high school in order to identify the factors related to smoking. Also, in case of smokers, their smoking amount, their smoking frequency, their first experience of smoking, their attempt of quitting smoking, etc were examined to find out the state of smoking among female college students. Also, as the core aspect of this study, the relationship of self-esteem and depression with smoking was investigated. As a result, the examination of smoking's effects on depression and self-esteem showed that the stud y subjects in the smoker group had a higher level of depression, indicating that smoking is related to the degree of depression (p-value = .017) (smoker group average: 14.75, non-smoker group average: 11.28). In the aspect of self-esteem, it was shown that the more self-esteem the subjects have, the more frequently they smoke, suggesting that whether or not people smoke is not related to the degree of their self-esteem (p-value = .017).

Potato (Solanum tuberosum L. cv. Gogu valley) protein as an antimicrobial agent in the diets of broilers.

Ohh, S H,Shinde, P L,Jin, Z,Choi, J Y,Hahn, T-W,Lim, H T,Kim, G Y,Park, Y,Hahm, K-S,Chae, B J Poultry Science Association, etc 2009 Poultry science Vol.88 No.6

<P>Two experiments were conducted to evaluate potato protein (PP, experiment 1) and refined PP (RPP, experiment 2) obtained from Gogu valley tubers as an antimicrobial agent in broiler diets. In both the experiments, 1-d-old male Ross 308 chicks were allotted to 5 treatments and performance, nutrient retention, and microbial populations in excreta and cecum were studied. Dietary treatments were as follows: basal diet (negative control, NC), basal diet with antibiotic (positive control, PC, 10 mg/kg of avilamycin), and low, medium, or high levels of PP (0.25, 0.50, and 0.75%, respectively, in experiment 1) or RPP (200, 400, and 600 mg/kg, respectively, in experiment 2). The overall gain and retention of DM (d 20 to 21) and CP (d 20 to 21 and d 41 to 42) were greater in birds fed PC and high PP diets than birds fed the NC diet. Population of total aerobic bacteria and coliforms was lowest in the cecum and excreta of birds fed the PC diet and highest in birds fed the NC diet. An increase in dietary PP linearly improved BW gain, feed intake, and feed conversion ratio during starter phase and overall BW gain. Also, there was linear improvement in retention of DM (d 20 to 21) and CP (d 20 to 21 and d 41 to 42) and reduced populations of total aerobic bacteria and coliforms in the cecum (d 42) and excreta (d 28 and 42) due to an increase in dietary PP. In the second experiment, the PC diet and diets with increasing levels of RPP had no effect on performance and nutrient retention. Birds fed the PC diet had the lowest microbial population in excreta and cecum, whereas the population of total aerobic bacteria and coliforms in excreta and cecum decreased (linear, P < 0.05) as the level of RPP was increased in the diet. These results suggest that both PP and RPP obtained from Gogu valley potato tubers have in vivo antimicrobial activity.</P>

왕고들빼기"선향"MAP에 적합한 저장온도 및 OTR 필름 구명

노희선 ( H S Noh ),임예나 ( Y N Lim ),임상현 ( S H Lim ),김희연 ( H Y Kim ),김상수 ( S S Kim ),최인이 ( I L Choi ),강호민 ( H M Kang ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2015 강원 농업생명환경연구 Vol.27 No.3

These studies were conducted to identify the proper temperature and the effects of laser ablation OTR (oxygen transmission rate) film on storability of Lactuca indica L.‘Seonhyang’. Respiration rate in 2℃ and 8℃ was low (20CO2mg/kg/hr). We used 3,000 cc, 5,000 cc, 7,000 cc and 10,000 cc/m2·day·atm OTR films in this research. Fresh weight loss rate was less than 1.0% in all OTR films and storage temperatures. This was lower in the low temperature. The change of oxygen and carbon dioxide contents in packing show the normal trend. The more oxygen transmission rate rises, the more oxygen contents rises. On the contrary to this, the carbon dioxide contents became low. The more temperature rise, the less oxygen contents and the more carbon dioxide contents became. Visual quality had the long storage times for sail in the low storage temperature and the low oxygen transmission rate film. Following these results, it comes to conclusion the suitable type of OTR film such as 3,000 cc and 5,000 cc for MA storage of Lactuca indica L.‘Seonhyang’ in 2℃ low temperature and storage time was 40 days.

Phase II study of preoperative chemoradiation with S-1 plus oxaliplatin in patients with locally advanced rectal cancer

Lee, E. M.,Hong, Y. S.,Kim, K. p.,Lee, J. L.,Kim, S. Y.,Park, Y. S.,Choi, D. H.,Kim, J. H.,Lim, S. B.,Yu, C. S. Blackwell Publishing Ltd 2013 CANCER SCIENCE Vol.104 No.1

Enhanced biomass and lipid production by supplement of myo-inositol with oceanic microalga Dunaliella salina

Cho, K.,Kim, K.N.,Lim, N.L.,Kim, M.S.,Ha, J.C.,Shin, H.H.,Kim, M.K.,Roh, S.W.,Kim, D.,Oda, T. Pergamon ; Elsevier Science Ltd 2015 Biomass & bioenergy Vol.72 No.-

We investigated the effects of inositols, which are well-known plant growth-promoting agents, on the growth of the oceanic microalga Dunaliella salina. Of the four inositol derivatives tested (myo-inositol, scyllo-inositol, d-chiro-inositol, and l-chiro-inositol), myo-inositol (MI) showed the greatest growth-promoting effect in a concentration-dependent manner. The yield of biomass from the alga cultured with 500 mg L<SUP>-1</SUP> of MI was 1.48-times that of the control culture. No significant effect of MI on the total carotenoid content was observed, but neutral lipid content was significantly increased, 1.34-times greater than the control. MI also influenced the fatty acid methyl ester composition, with the levels of linoleic, linolenic, and linolelaidic acids significantly higher than those of the control culture. To the best of our knowledge, this is the first demonstration that MI promotes the growth of a marine microalga. Our results suggest that MI has potential for enhancing the efficiency of biofuel production by D. salina through growth promotion and increasing lipid productivity.

배관해석에 의한 원전 배관부의 검사부위 선정

임형택,이삼래,이종포,김병철 한국비파괴검사학회 1992 한국비파괴검사학회지 Vol.12 No.2

Stress analysis has been performed using computer program ANSYS in the pressurizer surge line in accordance with ASME Sec. Ⅲ in order to predict possibility of fatigue failure due to thermal stratification phenomena in pipes connected to reactor coolant system of nuclear power plants. Highly vulnerable area to crack generation has been chosen by the analysis of fatigue due to thermal stress in pressurizer surge line. This kind of result can be helpful to choose the location requiring intensive care during inservice inspection of nuclear power plants.