JBR(Jeju Island Bovine Rotavirus)의 Sequencing에 의한 Homology 비교에 관한 연구

이종익,谷口孝喜,김응률,류영수,박선오,송진욱,유제현,조홍찬,차광종,浦澤正三,이태협,김유성,이영건 건국대학교 동물자원연구센터 1998 動物資源硏究誌 Vol.19 No.-

본 연구는 PCR에 의해 로타바이러스의 serotype을 결정하고 염기배열의 유사성을 다른 지역에서 발견된 로타바이러스와 비교 분석하였으며 그 결과는 다음과 같다. 1. 제주도 목장에서 송아지 설사변을 채취하여 로타바이러스를 분리 후 MA104세포에 감염시켜 세포변성 효과(CPE)를 확인하였다. 2. 전자현미경에 의해 형태학적로 로타바이러스임이 판명되었으며 형광항체법에 의해 MA104세포에 감염된 것을 재확인 하였다. 3. 제주도 송아지 로타바이러스(JBR)RNA를 PAGE에 의해 genotype를 분석한 결과 bovine특유의 4:2:3:2 pattern이지만 일반 PAGE로 분석한 결과는 NCDV, UK, KK3, A5-37, 61A, B223와 차이가 있었으며, Ns-5, Nc-5, Kawatabi(Japan)와는 비슷한 경향을 보였다. 4. Plaquing 후 titer한 결과 NCDV, UK보다는 낮은 ??PFU/ml이었다. 5. RNA-RAN hybridization과 ELISA 및 VP7과 VP4의 1차, 2차 PCR 사물을 1% agarose(EtBr 1㎕의 TAE)에서 전기영동한 결과 G6P11의 serotype이었다. 6. JBR의 P serotype이 동일하게 나타난 B223과의 비교 결과 총 JVP8 750 bases 중에서 731개의 염기가 B223과 같은 것으로 나타나 염기배열의 유사성은 97.47%로 나타났고, 아미노산 배열의 유사성은 97.57%로 나타났다. This study was carried our to identify JBR's serotype by polymerase chain reaction(PCR) and to analyze homology of JBR' sequence. The results obtained in these experiments were as follows: 1. Fecal samples of calf diarrhea were taken on farms in Jeju island, rotavirus was isolated and cytopathic effect(CPE) was determined after infection to MA104 cell. 2. Morphological evaluation on electron microscopy proved it as rotavirus. Also, its infection to MA104 cell was reidentified using a fluorescence antibody method. 3. Genotype of Jeju island bovine rotavirus(JBR) analyzed thorough PAGE was 4:2:3:2 pattern, which was unique in bovine and that analyzed through general PAGE was somewhat different from NCDV, UK, KK3, A5-37, 61A, B223 and similar to Nstool-5, N cultrue-5 and Kawatabi(Japan). 4. By titration after plaquing, the level was ??PEU/ml, which was lower than those of NCDV and UK. 5. Electrophoresis analysis of RNA-RNA hybridization. ELISA, adn first and second PCR products of VP7 and VP4 in 1% agarose(TAE+1㎕ EtBr) revealed that the rotavirus was a serotype of G6P11. 6. Alignment of two(JBR and B223) amino acid sequences is 97.57% and nucleotide sequences is 97.47%.

치즈 제조 부산물을 이용한 ELISA Blocking Agent의 개발에 관한 연구

유제현,송진욱,조흥찬,차광종,박범석,김응률,이종익,이중복,谷口孝喜 건국대학교 동물자원연구센터 1999 動物資源硏究誌 Vol.20 No.-

본 연구는 유청과 탈지분유를 blotto로 이용하여 ELISA 수행시 항원·항체반응에 비특이반응을 막는 Blocking agent를 탐색하기 위해 실험을 수행했다. MAb의 역가측정 결과 유의차가 인정되는 OD값이 0.3이상 검색된 희석배율은 1B2의 경우 819,200배, 6D4의 경우 1,638,400배, 15B10의 경우 204,800배이었다. 서로 다른 Blotto가 항원·항체반응에 미치는 영향을 각기다른 MAb를 이용하여 조사해 본 결과 MAb Yo-156, Yo-5는 Blocking agent로 표준적으로 사용하고 있는 BAS가 비교하여 group A 바이러스 7종 모두에 비슷한 정도의 발색을 일으켰으며, MAb G6는 G6 type바이러스 4종 모두에서 비슷한 정도의 발색을 일으켰고, MAb G10은 G10 type 바이러스 3종 모두에서 비슷한 정도의 발색을 일으켰다. 따라서 본 실험에 사용한 모든 종류의 blotto소재인, 탈지분유1(서울우유(협)), 탈지분유2((주)매일유업), 혼합탈지분유((주)매일유업), whey powder1(Sigma), whey powder2 ((주)삼익유가공), WPC34((주)환이상사), WPC800((주)후드피아), WPC8000 ((주)후드피아), WPI((주)환이상사)는 현재 Blocking agent로 사용하고 있는 BSA와 비교하여 유사하게 비특이 반응을 막아주는 것으로 밝혀져 경제적으로 보다 저렴한 blotto 소재로 이용이 가능하다는 사실을 확인하였다. Enzyme-Linked-Immuno-Sorbent-Assay (ELISA) has been frequently used for detection of interaction of antigen and antibody. Many efforts have been made to reduce nonspecific reaction of ELISA. Bovine serum albumin (BSA) is usually used for blocking the nonspecific reaction even thought it is expensive. Recently skin milk has been focused on blocking nonspecific reaction of ELISA. This study aimed to substitute expensive blocking agent of ELISA with cheap by-product of cheese manufacture. To evaluate the substitution of BSA with wheys made in different companies, ELISA was carried out using monoclonal antibodies (MAbs) against rotavirus. The blocking capacity of the whey was compared with that of the BSA. The commercial wheys showed the same activity as BSA when ELISA was performed using MAb YO-156, S2-37, and Yo-5. To further investigate whether the whey affects the detection specificity of MAb, the ELISA was carried out using type specific MAbs. MAb G6 specific to serotype G6 bound to only rotavirus serotype G6 such as NCDV, UK, JBR, and Jedong strain whereas MAb G10 specific to serotype G10 bound to serotype G10 rotavirus only, demonstrating that the whey a and no effect on the binding specificity of MAb with respect to antigen-antibody reaction. These data indicate that the whey may have no effect on specific interation of antigen-antibody and may be widely used for blocking substitute because of competitive priority of its price.

Optical Spectroscopic Investigations on Multiferroic MnWO₄

W. S. Choi,K. Taniguchi,S. J. Moon,S. J. Kim,S. S. A. Seo,Y. S. Lee,T. Arima,J. Y. Kim,H. Hoang,I.-S. Yang,T. W. Noh 한국자기학회 2008 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

FREE PAPAR ( ROOM 2 ) / B - 1 : Mite Survey in Asthmatic Chilren`s House

( S . Sasaki,M . Motonaga,K . Taniguchi,M . Yoshida ) 대한천식 및 알레르기학회 ( 구 대한알레르기학회 ) 1991 한일합동심포지움 Vol.11 No.1

Reduction of AOTF Insertion Loss

S. Taniguchi,M. Doi,T. Nakazawa,Y. Takasu,M. Seino 한국통신학회 1997 OPTOELECTRONICS & COMMUNICATIONS CONFERENCE Vol.2 No.1

Controlled Electrochemical Intercalation of Graphene/<i>h-</i>BN van der Waals Heterostructures

Zhao, S. Y. Frank,Elbaz, Giselle A.,Bediako, D. Kwabena,Yu, Cyndia,Efetov, Dmitri K.,Guo, Yinsheng,Ravichandran, Jayakanth,Min, Kyung-Ah,Hong, Suklyun,Taniguchi, Takashi,Watanabe, Kenji,Brus, Louis E. American Chemical Society 2018 Nano letters Vol.18 No.1

<P>Electrochemical intercalation is a powerful method for tuning the electronic properties of layered solids. In this work, we report an electrochemical strategy to controllably intercalate lithium ions into a series of van der Waals (vdW) heterostructures built by sandwiching graphene between hexagonal boron nitride (<I>h</I>-BN). We demonstrate that encapsulating graphene with <I>h</I>-BN eliminates parasitic surface side reactions while simultaneously creating a new heterointerface that permits intercalation between the atomically thin layers. To monitor the electrochemical process, we employ the Hall effect to precisely monitor the intercalation reaction. We also simultaneously probe the spectroscopic and electrical transport properties of the resulting intercalation compounds at different stages of intercalation. We achieve the highest carrier density >5 × 10<SUP>13</SUP> cm<SUP>2</SUP> with mobility >10<SUP>3</SUP> cm<SUP>2</SUP>/(V s) in the most heavily intercalated samples, where Shubnikov-de Haas quantum oscillations are observed at low temperatures. These results set the stage for further studies that employ intercalation in modifying properties of vdW heterostructures.</P> [FIG OMISSION]</BR>

Electronic structure and anomalous band-edge absorption feature in multiferroicMnWO4: An optical spectroscopic study

Choi, Woo Seok,Taniguchi, K.,Moon, S. J.,Seo, S. S. A.,Arima, T.,Hoang, H.,Yang, I.-S.,Noh, T. W.,Lee, Y. S. American Physical Society 2010 Physical review. B, Condensed matter and materials Vol.81 No.20

Regulation of Major Histocompatibility Class Ⅱ Gene Expressino in FRTL-5 Thyrocytes : Opposite Effects of Interferon and Methimazole

MONTANI, VALERIA,SHONG, MINHO,TANIGUCHI, SHIN-ICHI,SUZUKI, KOICHI,GIULIANI, CESIDIO,NAPOLITANO, GIORGIO,SAITO, JUN,SAJI, MOTOYASU,FIORENTINO, BRUNO,REIMOLD, ANDREAS M.,SINGER, DINAH S.,KOHN, LEONARD D 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

Aberrant expression of major histocompatibility complex (MHC) classⅡ antigens is associated with autoimmune thyroid disease; aberrant expression duplicating the autoimmune state can be induced by interferon-γ (IFNγ). We have studied IFNγ-induced human leukocyte antigen (HLA)-DRα gene expression in rat FRTL-5 thyroid cells to identify the elements and factors important for aberrant expression. Using an HLA-DRα 5'-flanking region construct from-176 to +45 bp coupled to the chloramphenicol acetyltransferase reporter gene, we show that there is no basal classⅡ gene expression in FRTL-5 thyroid cells, that IFNγ can induce expression, and, as is the case for antigen-presenting cells from the immune system, that IFNγ-induced expression requires several highly conserved elements on the 5'-flanking region, which, from 5' to 3', are the S, X_1, X_2, and Y boxes. Methimazole (MMI), a drug used to treat patients with Graves' disease and experimental thyroiditis in rats or mice, can suppress the IFNγ-induced increase in HLA-DRα gene expression as a function of time and concentration; MMI simultaneously decreases IFNγ-induced endogenous antigen presentation by the cell. Using gel shift assays and the HLA-DRα 5'-flanking region from -176 or -137 to +45 bp as radiolabeled probes, we observed the formation of a major protein-DNA complex with extracts from FRTL-5 cells untreated with IFNγ, termed the basal or constitutive complex, and formation of an additional complex with a slightly faster mobility in extracts from cells treated with IFNγ. MMI treatment of cells prevents IFNγ from increasing the formation of this faster migrating complex. Formation of both complexes is specific, as evidenced in competition studies with unlabeled fragments between -137 and -38 bp from the start of transcription; nevertheless, they can be distinguished in such studies. Thus, high concentrations of double stranded oligonucleotides containing the sequence of the Y box, but not S, X_1, or X_2 box sequences, can prevent formation of the IFNγ-increased faster migrating complex, but not the basal complex. Both complexes involve multiple proteins and can be distinguished by differences in their protein composition. Thus, using specific antisera, we show that two cAMP response element-binding proteins, activating transcription factor-1 and/or -2, are dominant proteins in the upper or basal complex. The upper or basal complex also includes c-Fos, Fra-2, Ets-2, and Oct-1. A dominant protein that distinguishes the IFNγ-increased lower complex is CREB-binding protein (CBP), a coactivator of cAMP response element-binding proteins. We, therefore, show that aberrant expression of MHC classⅡ in thyrocytes, induced by IFNγ, is associated with the induction or increased formation of a novel portein-DNA complex and that its formation as well as aberrant classⅡ expression are suppressed by MMI, a drug used to treat human and experimental autoimmune thyroid disease. Its component proteins differ from those in a major, basal, or constitutive protein-DNA complex formed with the classⅡ 5'-flanking region in cells that are not treated with IFNγ and that do not express the classⅡ gene. (Endocrinology 139: 290-302, 1998)

A Design of PFC Circuit for Resucing the Harmonic in Constant Voltage-fed Electronic Ballast Circuit

T.K.Kang,K.H.Koh,S.C.Moon,S.S.Noh,H.W.Lee,K. Taniguchi 전력전자학회 2004 ICPE(ISPE)논문집 Vol.- No.-

In this paper, a PFC(Power Factor Correction) electronic ballast with constant voltage-fed is proposed. The proposed PFC electronic ballast is combined of a highefficiency boost converter and a conventional half bridge inverter. It is proved that the ripple of input-current and the input-current's harmonic of the proposed PFC electronic ballast are reduced using the voltage divider and softswitching technique. It is demonstrated that simulation results for fluorescent lamp correspond with theoretical analysis.

THE HST COSMOS PROJECT: CONTRIBUTION FROM THE SUBARU TELESCOPE

TANIGUCHI YOSHIAKI,SCOVILLE N. Z.,SANDERS D. B.,MOBASHER B.,AUSSEL H.,CAPAK P.,AJIKI M.,MURAYAMA T.,MIYAZAK S.,KOMIYAMA Y.,SHIOYA Y.,NAGAO T. The Korean Astronomical Society 2005 Journal of The Korean Astronomical Society Vol.38 No.2

The Cosmic Evolution Survey (COSMOS) is a Hubble Space Telescope (HST) treasury project. The COSMOS aims to perform a 2 square degree imaging survey of an equatorial field in I(F814W) band, using the Advanced Camera for Surveys (ACS). Such a wide field survey, combined with ground-based photometric and spectroscopic data, is essential to understand the interplay between large scale structure, evolution and formation of galaxies and dark matter. In 2004, we have obtained high-quality, broad band images of the COSMOS field (B, V, r', i', and z') using Suprime-Cam on the Subaru Telescope, and we have started our new optical multi-band program, COSMOS-21 in 2005. Here, we present a brief summary of the current status of the COSMOS project together with contributions from the Subaru Telescope. Our future Subaru program, COSMOS-21, is also discussed briefly.