Salmonella Typhi, Salmonella Enteritidis 및 Salmonella Typhimurium균에 대한 Phage typing 및 antibiogram 분석

신영학,유정식,오경수,이점규,이상원,고운영,양병국 국립보건연구원 2000 국립보건원보 Vol.37 No.-

각종 갑상선질환에서의 항Thyroglobulin 항체 및 항Microsome 항체의 출현빈도에 관한 연구

이문호,고창순,이홍규,이권전,김병국,최강원,박성회,지제근,이상국 대한핵의학회 1979 핵의학 분자영상 Vol.13 No.1

저자들은 각종 갑상선질환에서 갑상선자가항체의 출현빈도를 조사하여 이들 항체가 갖는 진단적 가치를 관찰하고저, 1978년 4월부터 1979년 4월사이에 서울대학교병원 내과에 내원하였던 각종 갑상선질환환자 108명과 정상인 15명을 대상으로 하여, 감작혈구응집반응을 이용하여 항 thyroglobulin 항체와 microsome 항체를 측정하여 다음과 같은 결론을 얻었다. 1) 정상인 15명에서의 항 thyroglobulin 항체는 모두 음성이었는데, 항 microsome 항체는 1명(6.7%)에서 양성이었다. 각종 갑상선질환환자 108명에서의 결과는 각각 34명(31.5%) 및 40명(37.0%)이 양성이었다. 2) Graves병 환자 25명에서는 항 thyroglobulin 항체는 7명(28.0%)이 양성이었으나 항 microsome 항체는 9명(36.0%)에서 양성이었고, 음성인 군과 양성군 사이에 임상소견 및 검사소견이 특별한 차이를 볼 수 없었다. 3) Hashimoto병 환자는 18명으로 이들은 모두 침생검소견으로 확진된 예들로서, 두가지 항체가 각각 16명(88.9%)과 17명(94.4%)에서 양성이었다. 4) Familial goiter 환자는 7명이었는데 각각 4명(57%) 및 6(85%)명에서 양성이었다. 5) 양성갑성선종 35명에서는 각각 2명(5.7%) 및 3명(8.6%)이 양성의 소견을 보였으며, 갑상선암종 16명에서는 각각 3명(18.8%)이 양성이었다 6) 2명의 아급성갑상선염환자는 두가지가 모두 음성이었던데 반하여, 원발성점액수종환자 1명은 수검검사에서 양성이었다. 7) 1:802 이상의 고력가를 보인 예는 항 thyroglobulin 항체의 경우는 16명(14.8%)이었고, 이중 10명(62.5%)이 Hashimoto병이었으며, 항 microsome항체의 경우는 20명중 13명(65.0%)이 Hashimoto병이었다. 8) Tg Ab와 Mc Ab의 상관계수는 0.76으로 상관관계가 비교적 높으며 Mc Ab의 양성빈도가 약간 높았다. 이상의 결과를 종합하면 감작혈구응집반응은 간편하고 예민하며 신뢰도 및 재현성이 높은 검사로 생각되며 이 방법으로 측정한 항 thyroglobulin 항체 및 항 microsome 항체는 Hashimoto병에서 그 검출빈도가 높고 그 역가도 높아 Hashimoto병의 진단에 유용하다고 사료된다. The authors investigated the incidence of antithyroglobulin antibodies and antibodies and antimicrosomal antibodies measured by tanned red cell hemagglutination method in subjects suffering from various thyroid disorders. 1) In 15 normal patients, neither suffering from any thyroid diseases nor from any other autoimmune disorders, the antithyroglobulin antibodies were all negative, but the antimicrosomal antibody was positive only in one patient(6.7%). 2) The antithyroglobulin antibodies were positive in 31.5%(34 patients) of 108 patients with various thyroid diseases, and the antimicrosomal antibodies were positive in 37.0%(40 patients). 3) of the 25 patients with Graves' diseases, 7 patients(28.0%) showed positive for the antithyroglobulin antibodies, and 9(36.0%) for the antimicrosomal antibodies. There was no definite differences in clinical and thyroid functions between the groups with positive and negative results. 4) Both antibodies were positive in 16(88.9%) and 17(94.4%) patients respectively among 18 patients with Hashimoto's thyroiditis, all of them were diagnosed histologically. 5) Three out of 33 patients with thyroid adenoma showed positive antibodies, and 3 of 16 patients with thyroid carcinoma revealed positive antibodies. 6) TRCH antibodies demonstrated negative results in 2 patients with subacute thyroiditis, but positive in one patient with idiopathic primary myxedema. 7) The number of patiencs with high titers($gt;l:802) was 16 for antithyroglobulin antibody, and 62.5%(10 patients) of which was Hashimoto's thyroiditis. Thirteen(65.0) of 20 patients with high titers($gt;l:802) for antimicrosomal antibody was Hashimoto's thyroiditis. TRCH test is a simple, sensitive method, and has high reliablity and reproducibility. The incidences and titers of antithyroglobulin antibody and antimicrosomal antibody are especially high in Hashimoto's thyroiditis.

Establishment and characterization of cell lines from three human thyroid carcinomas: Responses to all-trans-retinoic acid and mutations in the BRAF gene

Koh, C.S.,Ku, J.L.,Park, S.Y.,Kim, K.H.,Choi, J.S.,Kim, I.J.,Park, J.H.,Oh, S.K.,Chung, J.K.,Lee, J.H.,Kim, W.H.,Kim, C.W.,Cho, B.Y.,Park, J.G. North-Holland 2007 Molecular and cellular endocrinology Vol.264 No.1

We report the characteristics of three cell lines (designated, SNU-80, SNU-373 and SNU-790), which were established from two papillary carcinomas and one anaplastic carcinoma obtained from three Korean thyroid carcinoma patients. All cell lines grow as adherent cells. Electron microscopy characteristically showed cytoplasmic invaginations of nuclei and intranuclear cytoplasmic inclusions. SNU-80 and SNU-790 cells showed a positive reaction to anti-cytokeratin antibody, and SNU-790 cells positivity for CK-19. All lines were free of mycoplasma or bacteria and were proven unique by DNA fingerprinting analysis. The p15 and p16 genes are deleted in the SNU-790 line. Mutations of the p53 gene were found in two lines (SNU-80 and SNU-373), but no mutations in the RET or MEN1 genes were observed. Mutations of the BRAF gene were found in the SNU-80 (G468R) and the SNU-790 (V599E) cell lines, but no mutations in the K-ras gene were present. SNU-80 and SNU-790 cells showed a positive reaction to anti-cytokeratin antibody, and no evidence of the production of thyroglobulin or calcitonin was observed. The cell lines were unable to trap radioactive iodine but did not contain TSH receptor. In addition, we investigated the mRNA expression levels of Tg, TSHR, TTF-1, PAX-8, NIS, IL-6, and LIF, and of the α, β and γ retinoic acid receptors in these cell lines. IL-6 was down-regulated in all three cell lines by all-trans-retinoic acid treatment. RAR-α was expressed but RAR-β was not expressed in the three cell lines, and RAR-γ was not expressed in SNU-790. Interestingly, RAR-β (SNU-80 and SNU-373) and RAR-γ (SNU-790) was up-regulated by all-trans-retinoic acid treatment. We believe that these well-characterized thyroid carcinoma cell lines may be useful tools for investigations on the biological characteristics of thyroid carcinoma, particularly for investigations related to gene alterations, especially of the BRAF gene. These cell lines may also be useful for redifferentiation therapy studies on thyroid carcinoma using all-trans-retinoic acid.

干拓地의 農業開發을 위한 綜合硏究 : III.界火干拓地에 있어서 鹽耐性 適樹의 選拔에 關하여 Selection of Some Nonhalophyte Species fit for Saline area at Gehwa Reclaimed land

高大植,金癸煥,위흡,韓光洙 전북대학교 농업과학기술연구소 1984 農大論文集 Vol.15 No.-

Twelve tree species growing near the reclaimed land of Kaehwa, Buan-Kun, Jeonlabug-Do province that were believed to be suitable for the windbreak and timber production, were selected and studied for their withering ratio and the contents of moisture, Na+, Cl-· and K+ in the roots of these trees. Results obtained are as follows: 1. The most suitable were found out to be Pinus Thunbergil, Robinia pseudo-acacia and Populus albaglandulosa, and the suitable were Populus euro-americanα. Koelreuteria pamculate acd Thuja ortentalis for planting in the Kaehwa reclaimed land. 2. The moisture content in the roots of Pinus Thunbergii, Robinia pseudo-acacia and Populus albaglandulosa, planted in the Kaehaw reclaimed land was remarkably low when compared to those planted in the none saline land. It was especially low during June through JuIy when relative humidity and the temperature were high. 3. In the roots of trees planted in the reclaimed land, Na+, and Cl-, content were relatively high in general. Na+ content was lowest during June through July but Cl- content was gradually increased from the beginning of the tree growth in May to the resting period of those tree growth. K+ content was remarkably decreased planted in saline area and gradually decreased in contents with times.

강원도 토교 3리에 대한 사회 의학적 조사

고현경,김혜숙,박순실,오혜숙,추희숙 梨花女子大學校 醫科大學 1975 梨花醫學誌 Vol.- No.8

Three Cases of Cutaneous Mesenchymal Hamartoma

Jang, K. A Korean Dermatological Association 1998 Annals of Dermatology Vol.10 No.4

각종 갑상선질환에서 혈청 myoglobin치에 관한 연구

정순일,조보연,이문호,장연복,고창순,조경삼,김삼용 대한핵의학회 1980 핵의학 분자영상 Vol.14 No.2

각종 갑상선질환에서 혈청 myoglobin치의 변화를 관찰하고 방사면역측정법으로 혈청 myoglobin치를 측정한 결과 다음과 같은 결과를 얻었다. 1) 치료전 갑상선기능항진증 환자의 혈청 myoglobin 평균치는 39.1±20.3 ng/ml로 정상대조군의 50.9±17.0 ng/ml보다 낮았으나 통계적으로 유의한 차이는 없었다. 2) 갑상선기능저하증 환자의 혈청 myoglobin치는 28∼156 ng/ml로 5예중 3예에서 증가되어 있었다. 3) 혈청 myoglobin치와 혈청 T₃, T₄치와는 유의한 상관관계가 없었다. 4) 갑상선중독성 근병증의 진단과 그 정도 및 치료후 경과관찰의 지표로서 유용할 가치가 있다고 사료된다. To evaluate the change of serum myoglobin in the thyroid disease, we measured serum myoglobin by radioimmunoassay in 24 normal subjects, 34 patients with hyperthyroidism (20 untreated, 14 treated) and 5 patients with hypothyroidism. The mean values of serum myoglobin in 24 normal control, 20 untreated hyperthyroidism and 14 treated hyperthyroidism were 50.9±17.0 ng/ml, 39.1±203 ng/ml and 51.0±18.3 ng/ml respectively. The serum myoglobin level of untreated hyperthyroidism seemed to be lower than that of normal control without statistical significance. The serum myoglobin levels in 5 patients with hypothyroidism ranged 25-156 ng/ml, and 3 patients showed increased serum myoglobin level. We observed one case with severe degree of thyrotoxic myopathy whose myoglobin level was markedly elevated initially and was decreased to normal range after treatment. This results suggests that the determination of serum myoglobin might be useful in the evaluation of thyrotoxic myopathy.

寒國 正常 新生兒의 末梢 赤血球 容積 및 白血球値의 變動에 關하여

金仲植,高舜姬,李慶孝,白承龍 대한소아과학회 1969 小兒科 Vol.12 No.7

化濃性 筋炎 1예

임효근,고순희,박승옥,최예원,김동규,정기석,류지소 대한감염학회 1990 감염 Vol.22 No.4

Pyomyositis is a bacterial infection of skeletal muscle usually occurring in the asence of trauma or a primary focus of infecation. Usual causative agent is Staphylococcus aureus. It is common in the tropics and rare in temperate climates. We diagnosed phomyositis in a 23-year-old man with symptoms of localized muscle pain, fever, chill and malaise. Muscular ultrasonogram and needle aspiration confirmed the disease. It was the first case reported in Korea.

아급성 갑상선염의 임상적 고찰

홍기석,고창순,정준기,이홍규,김삼용,석창호,박성회,김용일 대한핵의학회 1978 핵의학 분자영상 Vol.12 No.2

저자들은 서울대학교 병원 내과에서 108례의 갑상선 침생검을 시행하여 8례(7.9%)의 아급성갑상선염을 진단할 수 있었다. 이를 임상경과, 갑상선기능 등으로 본 임상단계와 비교한 결과 다음과 같은 결론을 얻었다. Czernick 등이 시도하였던 임상단계와 침생검의 조직소견이 비교적 잘 대응하였다. 임상경과가 길거나 갑상선기능이 낮을수록 갑상선의 섬유화가 심하였고 여포성분의 감소를 보였다. 갑상선의 기능상태와 옥소섭취율 혹은 갑상선주사상의 섭취율은 역 상관관계를 보였다. 임상단계의 초기에 발견된 환자들은 기능항진증의 증상을 보였다가 기능저하증의 양상을 보인 후 회복되는 이형적인 경과를 보였다. 임상단게의 후기에 발견된 환자들은 4명중 3명이 기능저하증을 보였는데, 이는 경과로 보아 질환의 최종단계로 생각되었다. $quot; 8 cases of subacute thyroiditis was diagraiosed amormg 108 needle biopsy of the thyroid during the period from April 1976 to August 1978. Correlation of the histologic findings with the clinical staging proposed by Czernick was relatively well matched; cases with long clinical course or low thyroid hormone levels showed greater fibrosis and reduction of follicular elements in thyroid. Thyroid (131)^I uptake or the uptake as seen on the thyroid scanning correlated inversely with the thyroid hormone levels. The clinical course of the patients seen in their initial stage of disease followed the classical pattern; hyperthyroid-like, hypothyroid-like and recovery. 3 among the 4, who were seen in their later clinical course showed hypothyroidism, which seemed the final outcome of their diseases.