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      • SCOPUSKCI등재

        Chlorella 엽록체의 인지질 생합성 및 지방산조성에 미치는 Nitrate와 Phosphate 결핍 효과

        이점규,李鍾三 한국식물학회 1988 Journal of Plant Biology Vol.31 No.3

        Chlorella ellipsoidea were cultured in "cold" media starvated with the nitrate and phosphate sources. The effects of the nitrate and phosphate starvation on the biosynthesis of phospholipid and the composition of fatty acids in chloroplasts isolated from these cells were analyzed. The syntheses of phosphatidylcholine and phosphatidylinositol in the nitrate and phosphate starvation were similarly inhibited as compared with the control but phosphatidyle-thanolamine synthesis in the nitrate starvation was extremely lower than that in the phosphate starvation. The major fatty acids utilized in phospholipid formation within chloroplasts were palmitic acid and linolenic acid. However, palmitic acid and stearic acid were dominant in the condition of the nitrate starvation. The levels of palmitic acid in the phosphate starvation were predominant and availability of palmitic acid were enhanced 3-fold than that of the control. These results suggest that the biosynthesis of phospholipid and the composition of fatty acids were affected by the nitrate and phosphate starvation in the culture media.

      • KCI등재후보

        1·2차 의료기관 임상 검체에서 분리된 Acinetobacter spp.의 항균제 내성과 Metallo-β-Lactamase 생성주 검출

        이점규,유용선,이영선,유재일,김봉수 대한감염학회 2004 Infection and Chemotherapy Vol.36 No.5

        Background:The aim of this study was to investigate the antimicrobial resistance of clinical isolates of Acinetobacter spp. collected from non-tertiary hospitals and to characterize the phenotype and the genotype of imipenem-non-susceptible isolates. Materials and Methods:Clinical isolates of Acinetobacter spp. were identified using recA-restriction fragment length polymorphism (RFLP) analysis with Tsp5091. Susceptibility to antimicrobial agents was determined using disk diffusion test and agar dilution test according to the criteria of the National Committee for Clinical Laboratory Standards. PCR and sequence analyses were used to detect the blaIMP-1 and blaVIM-2 genes, and to determine the content and order of the resistance genes inserted in integron. Results:Of 71 Acinetobacter spp. isolates collected from non-tertiary hospitals during 2002 and 2003, 60 isolates were A. baumannii, and 2, 4, and 5 isolates were Acinetobacter genomic species 3, 13TU, and A. lwoffii, respectively. The resistance rate of Acinetobacter spp. isolates to β- lactams, aminoglycosides, and fluoroquinolones was high except for imipenem and meropenem. The presence of blaVIM-2 gene was found in one isolate, Acinetobacter genomic species 13TU, for which the MIC of imipenem was 8 mg/L; the blaVIM-2 gene of this strain was located on 3 kb class 1 integron with aacA7 and aadA1 genes. Conclusions:Among the tested agents, imipenem and meropenem retained greatest activity against Acinetobacter spp. isolates collected from non-tertiary hospitals. This is the first report of VIM-2-producing Acinetobacter genomic species 13TU strains with class 1 integron containing blaVIM-2 gene. 결 론: Imipenem과 meropenem은 1 ․2차 의료기관의 임상 분리 Acinetobacter spp.에 대해 가장 큰 활성을 가지고 있었고, 국내 처음으로 확인된 VIM-2 생성 Acinetobacter genomic species 13TU 1주는 blaVIM-2 유전자를 포함하는 class 1 integron을 보유하고 있었다. 목 적 : 지역사회 내 1·2차 의료기관의 임상 검체에서 분리된 Acinetobacter spp.의 항균제 내성 실태를 파악하고 imipenem 비감수성 균주의 표현형 및 유전형 특성을 조사하고자 하였다.재료 및 방법 : 임상 검체에서 분리된 Acinetobacter spp.의 균종 동정은 제한효소 Tsp5091를 이용한 recA- RFLP법을 이용하였고, 항균제 감수성은 NCCLS 지침에 따라 원산확산법과 희석법으로 결정하였다. Imipenem 비감수성 Acinetobacter spp.를 대상으로 Hodge 변형법과 EDTA-double disk synergy 법을 실시하여 metallo-β- lactamase 생성 여부를 감별한 후 PCR과 염기서열분석으로 blaVIM-2와 blaIMP-1 유전자 존재와 integron의 유전자 서열 구조를 확인하였다. 결 과 : 2002년과 2003년 사이 1·2차 의료기관의 임상 검체에서 분리된 Acinetobacter spp. 71주 중 A. baumannii가 60주였고, Acinetobacter genomic species 3, 13TU와 A. lowffii 가 각각 2, 4, 5주씩 분리되었다. 임상 분리 Acinetobacter spp.는 imipenem과 meropenem를 제외한 β-lactam계, aminoglycoside계 와 fluoroquinolone계 항균제에 높은 내성을 나타내었다. Imipenem MIC가 8 mg/L 인 Acinetobacter genomic species 13TU 1주는 blaVIM-2 유전자 뒤에 aacA7와 aadA1 유전자가 배열한 3 kb 크기의 class 1 integron을 보유하고 있었다.

      • KCI등재후보

        1·2차 의료기관 임상 검체에서 분리된 Acinetobacter spp.의 항균제 내성과 Metallo-β-Lactamase 생성주 검출

        이점규,유용선,이영선,유재일,김봉수 대한감염학회 2004 감염과 화학요법 Vol.36 No.5

        목적 : 지역사회 내 1·2차 의료기관의 임상 검체에서 분리된 Acinetobacter spp.의 항균제 내성 실태를 파악하고 imipenem 비감수성 균주의 표현형 및 유전형 특성을 조사하고자 하였다. 재료 및 방법 : 임상 검체에서 분리된 Acinetobacter spp.의 균종 동정은 제한효소 Tsp5091를 이용한 reCA-RFLP법을 이용하였고, 항균제 감수성은 NCCLS 지침에 따라 원산확산법과 희석법으로 결정하였다. Imipenem 비감수성 Acinetobacter spp.를 대상으로 Hodge 변형법과 EDTA-double disk synergy 법을 실시하여 metallo-β-lactamase 생성 여부를 감별한 후 PCR과 염기서열분석으로 bla_(VIM-2)와 bla_(IMP-1) 유전자 존재와 integron의 유전자서열 구조를 확인하였다. 결과 : 2002년과 2003년 사이 1·2차 의료기관의 임상검체에서 분리된 Acinetobacter spp. 71주 중 A baumannii가 60주였고, Acinetobacter genomic species 3, 13TU와 A lowffii 가 각각 2, 4, 5주씩 분리되었다. 임상분리 Acinetobacter spp.는 imipenem과 meropenem를 제외한 β-1actam계, aminoglycoside계 와 fluoroquinolone계 항균제에 높은 내성을 나타내었다. Imipenem MIC가 8 mg/L 인 Acinetobacter genomic species 13TU 1주는 bla_(VIM-2) 유전자 뒤에 aacA7와 aadAl 유전자가 배열한 3kb 크기의 class 1 integron을 보유하고 있었다. 결론 : Imipenem과 meropenem은 1·2차 의료기관의 임상 분리 Acinetobacter spp.에 대해 가장 큰 활성을 가지고 있었고, 국내 처음으로 확인된 VIM-2 생성 Acinetobacter genomic species 13TU 1주는 bla_(VIM-2) 유전자를 포함하는 class 1 integron을 보유하고 있었다. Background : The aim of this study was to investigate the antimicrobial resistance of clinical isolates of Acinetobacter spp. collected from non-tertiary hospitals and to characterize the phenotype and the genotype of imipenem-non-susceptible isolates. Materials and Methods : Clinical isolates of Acinetobacter spp. were identified using recA-restriction fragment length polymorphism (RFLP) analysis with Tsp5091. Susceptibility to antimicrobial agents was determined using disk diffusion test and agar dilution test according to the criteria of the National Committee for Clinical Laboratory Standards. PCR and sequence analyses were used to detect the bla_(IMP-1) and bla(VIM-2) genes, and to determine the content and order of the resistance genes inserted in integron. Results : Of 71 Acinetobacter spp. isolates collected from non-tertiary hospitals during 2002 and 2003, 60 isolates were A. baumannii, and 2, 4, and 5 isolates were Acinetobacter genomic species 3, 13TU, and A. lwoffii, respectively. The resistance rate of Acinetobacter spp. isolates to β-lactams, aminoglycosides, and fluoroquinolones was high except for imipenem and meropenem. The presence of bla_(VIM-2) gene was found in one isolate, Acinetobacter genomic species 13TU, for which the MIC of imipenem was 8 mg/L; the bla(VIM-2) gene of this strain was located on 3 kb class 1 integron with aacA7 and aadA1 genes. Conclusions : Among the tested agents, imipenem and meropenem retained greatest activity against Acinetobacter spp. isolates collected from non-tertiary hospitals. This is the first report of VIM-2-producing Acinetobacter genomic species 13TU strains with class 1 integron containing bla_(VIM-2) gene.

      • KCI등재후보
      • A study on distribution and susceptibility to antifungal agents of Candida species isolated from hospitals and community health centers

        김영권 ; 김태운 ; 유재일 ; 최치원 ; 이점규 ; 김봉수 김천대학교 2004 김천대학교 논문집 Vol.25 No.-

        목적 : C. albicans 뿐만 아니라 non-albicans Candida의 감염 증가와 항진균제 내성 칸디다 종 출현이 세계적으로 보고되고 있다. 본 연구에서는 국내 1,2차 의료기관, 종합병원과 보건소에서 분리된 Candida 분리주를 대상으로 분리균의 분포를 조사하고 voriconazole을 포함한 4가지 항진균제에 대한 Candida spp.,의 감수성 분포를 조사하였다. he incidence of Candida albicans infections with non-albicans Candida and antifungal resistant Candida species have been increased worldwide. This study was carried out to determine Candida species dis-tribution and susceptibility to four antifungal agents including voricona zole against Candida isolates collected from hospitals and community health centers. Candida species identification was performed using the Vitek instru-ment or Candida CHROM agar medium. The MICs of antifungal agents for Candida species were determined by the NCCLS broth microdilution method and confirmed with E test method. In non-tertiary hospitals and community health centers, C. albicans was still the most common isolates, but incidence rate of non-albicans Candida isolated from blood specmens in tertiary hospitals was more than that of C. albicans. The fluconazole resistant Candida species were rare but the resistance to amphotericin B and 5 flucytosine was detected in C. albicans of all the institution. Most of Candida species showed low MICS (50%. MIC 0.03 ug/ml) to voriconazole compared to fluconazole.

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