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이경희,박혜련 명지대학교 대학원 1997 대학원논문집 Vol.1 No.-
This study was performed to assess the health and nutritional status of 125 elderly living in the Yongin area, Kyonggi-do. Survey questionnaires including 24-hr dietary recall, food habit, anthropometric measurements were applied as the instruments. The average age of the subjects were 77 for male and 75 for female. Mean value of BMI was 23.2. About 30% of the subjects was overweight, which is over 25 of BMI. Caloric intake and protein intake were about 50% of the Korean RDA. Their diets were inappropriate, showing C: P: F ratio = 75: 15: 10. Female elderly showed higher food habit score than male elderly(p<0.05).
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이인경,김희선,전경희,김성광,정시련 영남대학교 약품개발연구소 1995 영남대학교 약품개발연구소 연구업적집 Vol.5 No.-
The gene expressions of 5 cytokines(IL-1, IL-2, IL-6, TNFα and IFNy) from human peripheral blood mononuclear cells(PBMC) stimulated with Lentinus edodes lectin(LEL) were investigated by using reverse transcription polymerase chain reaction(RT-PCR). PBMC(1×10^(6) cells/ml) isolated from healthy volunteers were stimulated with LEL(O.D.=3.0) or lipopolysaccharide(LPS, 10㎎/ml) for various times(1, 8, 24, 48, 72, 96 and 120 hours). After each of the various stimulated times, total RNA was isolated by RNA sol B method and assessed for various cytokines mRNA by RT-PCR. The mRNA encoding IL-6 and IFNy were detected continuously from 1 to 120 hours. The mRNA encoding IL-1 were detected up to 72 hours and the intensity of the band were increased from 24 to 72 hours. The mRNA encoding IL-2 were detected from 8 to 120 hours and showed delayed reaction compare with other cytokines, But the mRNA encoding TNFα was detected up to 24 hours only. The patterns of TNfα gene expression showed short time response compared with other cytokines. These results suggest that LEL as an inducers of cytokines, can elicits a detectable cytokine on mRNA from the PBMC within the first few hours of stimulation.
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전경희,정시련,이인경,고성진 嶺南大學校 基礎科學硏究所 1993 基礎科學硏究 Vol.13 No.-
Table 1. Specificity of Rhizobium sp. crude lectin in agglutinating erythrocytes from various animals (그림) [HU]: hemagglutinatin unit Table 2. Specificity of intracellular crude lectin from Rhizobium sp. in agglutinating of erythrocytes from various animals (그림) [HU]: hemagglutinatin unit Table 3. Hemagglutination of intracellular crude lectin from Rhizobium sp. in culture age (그림) ※[HU]: hemagglutinatin unit (그래프) Fig.6 Stability of Rhizobium japonicum iectin activity at different pH. Rhizobium japonicum crude lectin was incubated in buffers at different pH(2.18-10.80) for 24hr at 4℃ and the remaining activity was determined. Table 4. Effect of mental ions and chelating agent in hemagglutinating activity for Rhizobium sp. crude lectin (그림) *Chelating agent (그래프) Flg.7 Stability of Rhizobium japonicum iectin activity at different temperature. Rhizobium japonicum was incubated for 30min. After immediate cooling in an ice-water bath, the remaining activity was determined. 조사한 결과 3일째 부터 약한 활성을 나타냈으며, 6일째에 32HU로 최대의 활성을 나타내었다. 배지로 분비된 렉틴은 사람 적혈구와 토끼 적혈구에서 응집현상을 나타내었으며 trypsin처리후 토끼와 DEAE 0.2M 분획에서 하나의 major band와 minor band가 나타났으며, Rhizobium sp. 렉틴의 활성은 pH 7-9범위에서 안정한 것으로 나타났으며, 또한 온도의 영향은 50℃이하에서 안정하였다. 적혈구 응집 저해 효과는 25mM galactose에서 최대로 나타났다. 세포내 결합형 렉틴을 배양시기(1, 2, 4, 6일)에 따라 전기 영동 시험을 실시한 결과 동일한 band pattern과 렉틴 활성을 나타냈으므로, 세포내 결합형 렉틴은 배양 시기 별로 차이가 없음을 알 수 있었다. Table 5. The comparison of protein and carbohydrate contents in Rhizobium sp. crude lectin (그림) a : YMA broth media b : Cell was ruptured by sonication. Crude lectins were partially purified from Rhizobium sp. by salt fractionation and ion exchange chromatography on DEAE-Sephadex A-50. Hemagglutination of extracts from culture of Rhizobium sp. with age showed remarkable activity in 6 days. With purified lectin several biochemical properties have been characterized: Rhizobium sp. lectin agglutinated nonspecifically erythrocytes of human and several animals. In SDS-polyacrylamide gel electrophoresis, Rhizobium sp. lectin (0.2M fraction of DEAE Sephadex A-50) showed one major and one minor bands. Crude lectin from Rhizobium sp. was relatively stable at the range fo pH 7-9 and temperature below 50℃. In sugar inhibition test, this lectin was inhibited by only galactose at final concentration of 25mM. Meanwhile, intracellular crude lectin from Rhizobium sp. with culture age showed same band patterns by polyacrylamide gel electrophoresis.
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신동륜,이준서,차경옥 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-
Based on Experimental analysis, the characteristics of back pressure in automotive exhaust system is tested for 4-stroke gasoline engine. The back pressure in automotive exhaust system is generated by resistance working of exhaust system, i.e. exhaust manifold, pipe length, pipe banding, difference system pressure with atmospheric pressure. This paper contains experimental results which are tested for the change of exhaust pipe length and torque change are tested under experimental conditions.
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감염 조절용 차단막의 두께가 광중합기의 중합광에 미치는 영향
장훈상,이석련,홍성옥,류현욱,송창규,민경산 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.5
연구목적: 본 연구는 감염 조절용 차단막을 여러 겹으로 사용했을 때 광중합기의 광강도와 파장, light diffusion 등에 미치는 영향에 대해 조사하였다. 연구 재료 및 방법: 감염 조절용 차단막은 투명 랩 (크린랩)을 사용하였고 광중합기는 할로겐 광중합기 (Optilux 360)와 LED 광중합기 (Elipar FreeLight 2)를 사용하였다. 차단막을 1겹, 2겹, 4겹, 8겹으로 광중합기의 광섬유말단을 감싸고 휴대용 광강도 측정기 (Cure Rite)로 광중합기의 광강도를 측정하였다. 광중합기를 주문제작한 optical breadboard에 고정시킨 후 휴대용 spectroradiometer (CS-1000)를 이용하여 광중합기의 파장을 측정하였고, DSLR (Nikon D70s)을 이용하여 광중합기의 light diffusion을 사진 촬영하였다. 결과: 광강도 측정 결과는 차단막의 두께가 증가할수록 광강도가 유의하게 감소하였으나 할로겐 광중합기에서 1겹과 2겹 사이에는 유의차가 없었으며, 4겹 이상의 차단막을 투과할 때 광강도가 더 많이 감소하였다. 여러 겹의 차단막을 투과한 광중합기의 전체적인 파장 형태와 peak wavelength의 변화는 관찰되지 않았다. Light diffusion 사진 촬영 시, LED 광중합기에서는 차단막의 두께가 미치는 영향이 없었으나 할로겐 광중합기에서는 차단막을 4겹 사용했을 때부터 중합광이 조사되는 각도가 감소하기 시작하여 8겹 사용했을 때 통계적으로 유의하게 감소하는 것을 볼 수 있었다 (p < 0.05). 결론: 광중합형 복합레진을 광중합할 경우 감염 조절용 차단막이 찢어지는 경우를 대비하여 1겹으로 사용하기 보다는 2겹으로 사용하는 것이 환자간의 교차감염을 예방하는데 유리할 것으로 사료된다. Objectives: This study investigated the effect of infection control barrier thickness on power density, wavelength, and light diffusion of light curing units. Materials and Methods: Infection control barrier (Cleanwrap) in one-fold, two-fold, four-fold, and eightfold, and a halogen light curing unit (Optilux 360) and a light emitting diode (LED) light curing unit (Elipar FreeLight 2) were used in this study. Power density of light curing units with infection control barriers covering the fiberoptic bundle was measured with a hand held dental radiometer (Cure Rite). Wavelength of light curing units fixed on a custom made optical breadboard was measured with a portable spectroradiometer (CS-1000). Light diffusion of light curing units was photographed with DSLR (Nikon D70s) as above. Results: Power density decreased significantly as the layer thickness of the infection control barrier increased, except the one-fold and two-fold in halogen light curing unit. Especially, when the barrier was four-fold and more in the halogen light curing unit, the decrease of power density was more prominent. The wavelength of light curing units was not affected by the barriers and almost no change was detected in the peak wavelength. Light diffusion of LED light curing unit was not affected by barriers, however, halogen light curing unit showed decrease in light diffusion angle when the barrier was four-fold and statistically different decrease when the barrier was eight-fold (p < 0.05). Conclusions: It could be assumed that the infection control barriers should be used as two-fold rather than one-fold to prevent tearing of the barriers and subsequent cross contamination between the patients.
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Lee, Myung Kyung,Lee, Woo Jin,Do, Young Rok,Lee, Keun Seok,Jung, Kyung Hae,Heo, Dae Seog,Kim, Sam Yong,Park, Sang Yoon,Jeong, Hyun Sik,Kang, Jung Hun,Kim, Si-Young,Park, Sook Ryun,Yun, Young Ho Cambridge University Press 2015 Palliative & supportive care Vol.13 No.4
<B>Abstract</B><B>Objective:</B><P>This study examined changes in health-related quality of life (HRQoL) and quality of care (QoC) as perceived by terminally ill cancer patients and a stratified set of HRQoL or QoC factors that are most likely to influence survival at the end of life (EoL).</P><B>Method:</B><P>We administered questionnaires to 619 consecutive patients immediately after they were diagnosed with terminal cancer by physicians at 11 university hospitals and at the National Cancer Center in Korea. Subjects were followed up over 161.2 person-years until their deaths. We measured HRQoL using the core 30-item European Organization for Research and Treatment of Cancer Quality of Life Questionnaire, and QoC using the Quality Care Questionnaire-End of Life (QCQ-EoL). We evaluated changes in HRQoL and QoC issues during the first three months after enrollment, performing sensitivity analysis by using data generated via four methods (complete case analysis, available case analysis, the last observation carried forward, and multiple imputation).</P><B>Results:</B><P>Emotional and cognitive functioning decreased significantly over time, while dyspnea, constipation, and pain increased significantly. Dignity-conserving care, care by healthcare professionals, family relationships, and QCQ-EoL total score decreased significantly. Global QoL, appetite loss, and Eastern Cooperative Oncology Group Performance Status (ECOG-PS) scores were significantly associated with survival.</P><B>Significance of results:</B><P>Future standardization of palliative care should be focused on assessment of these deteriorated types of quality. Accurate estimates of the length of life remaining for terminally ill cancer patients by such EoL-enhancing factors as global QoL, appetite loss, and ECOG-PS are needed to help patients experience a dignified and comfortable death.</P>
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