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Acremonium chrysogenum에 의한 Cephalosporin C 생산 및 모델링
이종일,류화원,박돈희 전남대학교 촉매연구소 2000 觸媒硏究 論文集 Vol.21 No.-
The production of cephalosporin C and its biosynthetic modeling were studied. Cultivation experiments were carried out in an agitated and aerated fermentor using Acremonium chrysogenum. Corn steep liquor was used as a major carbon source. Intracellular and extracellular concentrations of metabolites and enzymes were measured. Based on enzymatic kinetics the biosynthetic modeling of cephalosporin C was developed and used to compare the experimental data with simulation results. In the simulation the effect of dissolved oxygen concentraction on cephalosporin C production was investigated. For the high production yield of cephalosporin C the dissolved oxygen concentration should be kept over 40 % during the cultivation. The biosynthetic modeling can help further to understand the biosynthetic machinery of the microorganism to produce cephalosporin C.
Rhizopus oryzae KCTC 11970을 이용한 푸마르산 생산조건의 최적화
김진남,양환승,이종일,류화원 全南大學校 觸媒硏究所 2002 觸媒硏究 論文集 Vol.23 No.-
This work was developed in several steps to find the optimum conditions of fumaric acid production by Rhizopus oryzae KCTC 11970 in flask culture. Six different carbon sources and eleven nitrogen sources were tested. CaCO_3 was used as a neutralizing agent to control pH of medium. The optimum carbon source and nitrogen source were glucose 70 g/L and ammonium sulfate 0.4 g/L, respectively. At this optimum condition, the fumaric acid concentration, yield, and volumetric productivity were 19.33 g/L, 52.8%, and 0.32 g/L/h, respectivity, after fermentation time of 60 hr.
이석호,이화영,이규택,강인구,최규완,백승운,이종균,이준혁,고광철,이종철,오영륜,현재근,이풍렬,김재준,채종일 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.2
Strongyloidiasis is a parasitic disease caused by Strongyloides stercoralis which exists in two forms : the free living and parasitic forms. It exists in warm, moist climate in areas where there is frequent fecal contamination of the soil. After cutaneous invasion by the filariform larvae, petechial hemorrage, pruritus, papular rashes, edema, and urticaria occur. Infection commonly occurs in the proximal intestine of the gastrointestinal (G-I) tract but may extend from the stomach to the anus. Once the worm is established in the small intestine, the physical findings may include epigastric tenderness to palpation. The mucosal biopsy is an inefficient way of making the diagnosis because the worm is found in the biopsy specimen in only 2% of patients. Gastric strongyloidiasis is rare. We experienced a case of gastric strongyloidiasis diagnosed by the endoscopic biopsy and serologic test for parasite specific IgG antibody by micro-ELISA.