Genetic Variability of Sorghum Charcoal Rot Pathogen (Macrophomina phaseolina) Assessed by Random DNA Markers

Rajkumar,Fakrudin Bashasab,Mahaling S Kuruvinashetti 한국식물병리학회 2007 Plant Pathology Journal Vol.23 No.2

Genetic diversity among selected isolates of Macrophomina phaseolina, a causal agent of charcoal rot (stalk rot) disease in sorghum was studied using PCRRAPD markers. A set of ten isolates, from ten different rabi sorghum genotypes representing two traditional sorghum growing situations viz., Dharwad- a transitional high rainfall region and Bijapur- a semi-arid low rainfall region in South India. From a set of 40 random primers tested, amplicon profiles of 15 were reproducible. A total of 149 amplicon levels, with an average of 9.9 bands per primer, were available for analysis, of which 148 were polymorphic (99.3%). It was possible to discriminate all the isolates with any of the 15 primers employed. UPGMA clustering of data indicated that the isolates shared varied levels of genetic similarity within a range of 0.14 to 0.72 similarity coefficient index and it was suggestive that grouping of isolates was not related to sampling location in anyway. A high level of genetic heterogeneity of 0.28 was recorded among the isolates.

Analytical Modelling and Simulation Analysis of Junctionless Nanotube (JL NT) MOSFET

Raj Kumar,Raj Kumar,Arvind Kumar 한국전기전자재료학회 2022 Transactions on Electrical and Electronic Material Vol.23 No.4

In this paper, an analytical model for junctionless nanotube (JLNT) MOSFET has been developed. The analytical model for minimum central potential, threshold voltage and drain current has been developed by using variable separation method. These model expressions are further applied to analyse the Short Channel Effects (SCEs) of JL NT MOSFET. The electrical performance of proposed device has been investigated by varying different process parameters such as silicon nanotube thickness, gate oxide thickness, and gate length. All the results of developed models have been validated by comparing with so obtained simulated results from genius 3D device simulator of VisualTCAD for different device parameters. JLNT MOSFET with appropriate design parameters can be further explored for circuit applications.

Effect of process parameters on the recovery of thorium tetrafl uoride prepared by hydrofl uorination of thorium oxide, and their optimization

Raj Kumar,Sonal Gupta,Sourabh Wajhal,S.K. Satpati,M.L. Sahu 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.5

Liquid fueled molten salt reactors (MSRs) have seen renewed interest because of their inherent safetyfeatures, higher thermal efficiency and potential for efficient thorium utilisation for power generation. Thorium fluoride is one of the salts used in liquid fueled MSRs employing TheU cycle. In the presentstudy, ThF4 was prepared by hydro-fluorination of ThO2 using anhydrous HF gas. Process parameters viz. bed depth, hydrofluorination time and hydrofluorination temperature, were optimized for the preparation of ThF4 in a static bed reactor setup. The products were characterized with X-Ray diffraction andexperimental conditions for complete conversion to ThF4 were established which also corroborated withthe yield values. Hydrofluorination of ThO2 at 450 C for half an hour at a bed depth of 6 mm gave thebest result, with a yield of about 99.36% ThF4. No unconverted oxide or any other impurity was observed. Rietveld refinement was performed on the XRD data of this ThF4, and Chi2 value of 3.54 indicated goodagreement between observed and calculated profiles.

ENERGY AND EXERGY ANALYSIS OF COMPACT POWER GENERATION AND HYBRID SOLAR ENERGY-WASTE HEAT-BASED TRIPLE EFFECT EJECTOR-VAPOR ABSORPTION REFRIGERATION CYCLE

RAJ KUMAR,ANIL KUMAR 대한설비공학회 2013 International Journal Of Air-Conditioning and Refr Vol.21 No.4

An NH3–H2O ejector-absorption refrigeration cycle, and an R-152a ejector refrigeration cycle are employed with a renewable energy power generator to make a proposed compact power generation and triple effect ejector-absorption refrigeration cycle. The exergy analysis of the cycle leads to a possible performance improvement. Approximately 71.69% of the input exergy is destructed due to irreversibilities in different components. Around 7.976% is available as the useful exergy output. The exhaust exergy lost to the environment is 20.33%, which is lower than the exhaust energy loss of 47.95%, while the useful energy output is 27.88%. The refrigerants used are of zero ODP and negligible GWP, and the CO2 emission of the exhaust gases is very small as compared to that of the fossil fuel run engine, hence, this cycle is favorable to the global environment. The results also show that the proposed cycle has signi¯cant higher energy and exergy effciency than the earlier investigated `triple effect refrigeration cycle' and `the combined power and ejectorrefrigeration cycle'.

On harmonic convolutions involving a vertical strip mapping

Raj Kumar,Sushma Gupta,Sukhjit Singh,Michael Dorff 대한수학회 2015 대한수학회보 Vol.52 No.1

Let $ f_\beta=h_\beta+\overline{g}{_\beta}$ and $F_a=H_a+\overline{G}_a$ be harmonic mappings obtained by shearing of analytic mappings $$h_\beta+g_\beta={1}/{(2i{\sin}\beta)}\log\left({(1+ze^{i\beta})}/{(1+ze^{-i\beta})}\right),~0<\beta<\pi$$ and $H_a+G_a={z}/{(1-z)}$, respectively. Kumar \emph{et al.} \cite{ku and gu} conjectured that if $\omega(z)=e^{i\theta}z^n (\theta\in\mathbb{R},\,\, n\in \mathbb{N})$ and $ \omega_a(z)={(a-z)}/{(1-az)},\,a\in(-1,1)$ are dilatations of $f_\beta$ and $F_a$, respectively, then $F_a\widetilde\ast f_\beta \, \in S_H^0$ and is convex in the direction of the real axis, provided $a\in \left[{(n-2)}/{(n+2)},1\right)$. They claimed to have verified the result for $n=1,2,3$ and $4$ only. In the present paper, we settle the above conjecture, in the affirmative, for $\beta=\pi/2$ and for all $n\in \mathbb{N}$.

One-Pot Synthesis of Fluorescent Hydrogels by the In-Situ Growth of Carbon Dots on Holocellulose Nanofibrils

( Rajkumar Bandi ),( Chan-woo Park ),( Song-yi Han ),( Azelia Wulan Cindradewi ),( Eun-ah Lee ),( Jeong-ki Kim ),( Gu-joong Kwon ),( Seung-hwan Lee ) 한국목재공학회 2021 한국목재공학회 학술발표논문집 Vol.2021 No.1

Preparation of nanocellulose-based hydrogels that can be used in the field of biomedicine, have gained tremendous attention. Carbon dots are the latest addition to the carbon nanomaterials family and are well known for their stable fluorescence and high biocompatibility. Hence the incorporation of carbon dots into the nanocellulose-based hydrogels can broaden their applicability. In this work, a facile one-pot hydrothermal method is demonstrated to prepare N-doped carbon dots/holocellulose nanofibrils composite (NCDs/HCNF) hydrogel. During the hydrothermal treatment, the NCDs grow in-situ on HCNF surface and serve as cross-linkers to produce hydrogel. Hemicellulose portion of HCNF act as carbon source and ethylenediamine will serve as N dopant. Synthesis conditions were optimized, optical and physicochemical properties were studied in detail.

In-Situ Hydrothermal Synthesis of a MoS2 Nanosheet Electrode for Electrochemical Energy Storage Applications

Rajkumar Patel,Akbar I. Inamdar,Hyungbae Kim,Hyunsik Im,Hyungsang Kim 한국물리학회 2016 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.68 No.11

A molybdenum disulfide (MoS2) nanosheet film was grown directly on a stainless-steel substrate by using an in-situ hydrothermal growth technique at 200 C. The formation of an MoS2 hexagonal structure with a nanosheet-like morphology was confirmed by using X-ray diffraction (XRD) and field-emission scanning electron microscopy (FE-SEM) while a layered MoS2 nanosheet structure was observed under an energy-filtering transmission electron microscope (EF-TEM). The electrochemical supercapacitor properties of the MoS2 nanosheet electrode were measured in 1-M aqueous Na2SO4 electrolyte by using cyclic voltammetry (CV) and charge/discharge technique, and the electrode’s specific capacitances were 91.29 F/g and 146.15 F/g, respectively. The concurrent double-layer capacitance and pseudo-capacitance behaviors of the electrode manifested themselves in the rectangular shape and redox peaks of the CV curve. The mesoporous MoS2 nanosheets were electrochemically stable for up to 1000 charge/discharge cycles.

Design and Experimental Investigation of Zeta Converter Fed Reduced Switch Multi-stage Grid Connected Boost MLI

Rajkumar M.,Rathinam A. 대한전기학회 2024 Journal of Electrical Engineering & Technology Vol.19 No.1

Single-phase grid-tied inverter is a promising structure for low wattage sustainable Energy systems due to its compact size, fexible operation and lower cost. The traditional system employs a boost converter and a full bridge inverter to pump the energy from sustainable sources to the utility grid. The traditional boost converter has bounded operation in increasing or decreasing the dc voltage from sources like PV modules. The full bridge three-level PWM inverter, on the other hand, increases switching losses, devices with high voltage/current capacity, and electromagnetic interference.This work tries to address the faults highlighted earlier by introducing a modifed reduced switch count Multi-stage Boost Inverter. This setup has two stages, a zeta converter used for limitless operation across the working range and a reduced count multilevel inverter. This setup has ability to boost the input DC voltage with voltage self-balancing and convert to AC voltage. This setup has a current control technique is used to synchronize the inverter output sinusoidal and in-phase with the grid parameters. This technique is also used to control the duty cycle to maintain the required output voltage at converter side. Usefulness of the presented work is analyzed in MATLAB/Simulink and an experimental prototype is constructed to verify the simulated response.

One-Pot Synthesis of Ag Nanoparticles/Carbon Dots/Holocellulose Nanofibrils Ternary Nanocomposite

( Rajkumar Bandi ),( Chan-woo Park ),( Song-yi Han ),( Azelia Wulan Cindradewi ),( Eun-ah Lee ),( Jeong-ki Kim ),( Gu-joong Kwon ),( Seung-hwan Lee ) 한국목재공학회 2021 한국목재공학회 학술발표논문집 Vol.2021 No.1

Research on development of sustainable and environmentally friendly nanocomposites is a fast growing field due to their potential applications from the combination of unique chemical, optical and mechanical characteristics. Nanocellulose has a great potential to act as a sustainable support material for the formation of various nanocomposites. In this work, a novel ternary composite consisting of Ag nanoparticles, carbon dots and holocellulose nanofibrils (AgNPs/CDs/HCNF) is prepared by a one-pot hydrothermal treatment. During the reaction, AgNPs and CDs were grown in-situ on HCNF. Hemicellulose is crucial for the formation of CDs and AgNPs which was evident from control experiments using pure cellulose nanofibrils. Synthesis conditions were optimized, optical and physicochemical properties of the composite were studied in detail.

Targeted Resequencing of 30 Genes Improves the Detection of Deleterious Mutations in South Indian Women with Breast and/or Ovarian Cancers

Rajkumar, Thangarajan,Meenakumari, Balaiah,Mani, Samson,Sridevi, Veluswami,Sundersingh, Shirley Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.13

Background: We earlier used PCR-dHPLC for mutation analysis of BRCA1 and BRCA2. In this article we report application of targeted resequencing of 30 genes involved in hereditary cancers. Materials and Methods: A total of 91 patient samples were analysed using a panel of 30 genes in the Illumina HiScan SQ system. CLCBio was used for mapping reads to the reference sequences as well as for quality-based variant detection. All the deleterious mutations were then reconfirmed using Sanger sequencing. Kaplan Meier analysis was conducted to assess the effect of deleterious mutations on disease free and overall survival. Results: Seventy four of the 91 samples had been run earlier using the PCR-dHPLC and no deleterious mutations had been detected while 17 samples were tested for the first time. A total of 24 deleterious mutations were detected, 11 in BRCA1, 4 in BRCA2, 5 in p53, one each in RAD50, RAD52, ATM and TP53BP1. Some 19 deleterious mutations were seen in patients who had been tested earlier with PCR-dHPLC [19/74] and 5/17 in the samples tested for the first time, Together with our earlier detected 21 deleterious mutations in BRCA1 and BRCA2, we now had 45 mutations in 44 patients. BRCA1c.68_69delAG;p.Glu23ValfsX16 mutation was the most common, seen in 10/44 patients. Kaplan Meier survival analysis did not show any difference in disease free and overall survival in the patients with and without deleterious mutations. Conclusions: The NGS platform is more sensitive and cost effective in detecting mutations in genes involved in hereditary breast and/or ovarian cancers.