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Bashasab, Rajkumar, Fakrudin,Kuruvinashetti, Mahaling S The Korean Society of Plant Pathology 2007 Plant Pathology Journal Vol.23 No.2
Genetic diversity among selected isolates of Macrophomina phaseolina, a causal agent of charcoal rot (stalk rot) disease in sorghum was studied using PCR-RAPD markers. A set of ten isolates, from ten different rabi sorghum genotypes representing two traditional sorghum growing situations viz., Dharwad- a transitional high rainfall region and Bijapur- a semi-arid low rainfall region in South India. From a set of 40 random primers tested, amplicon profiles of 15 were reproducible. A total of 149 amplicon levels, with an average of 9.9 bands per primer, were available for analysis, of which 148 were polymorphic (99.3%). It was possible to discriminate all the isolates with any of the 15 primers employed. UPGMA clustering of data indicated that the isolates shared varied levels of genetic similarity within a range of 0.14 to 0.72 similarity coefficient index and it was suggestive that grouping of isolates was not related to sampling location in anyway. A high level of genetic heterogeneity of 0.28 was recorded among the isolates.
Rajkumar,Fakrudin Bashasab,Mahaling S Kuruvinashetti 한국식물병리학회 2007 Plant Pathology Journal Vol.23 No.2
Genetic diversity among selected isolates of Macrophomina phaseolina, a causal agent of charcoal rot (stalk rot) disease in sorghum was studied using PCRRAPD markers. A set of ten isolates, from ten different rabi sorghum genotypes representing two traditional sorghum growing situations viz., Dharwad- a transitional high rainfall region and Bijapur- a semi-arid low rainfall region in South India. From a set of 40 random primers tested, amplicon profiles of 15 were reproducible. A total of 149 amplicon levels, with an average of 9.9 bands per primer, were available for analysis, of which 148 were polymorphic (99.3%). It was possible to discriminate all the isolates with any of the 15 primers employed. UPGMA clustering of data indicated that the isolates shared varied levels of genetic similarity within a range of 0.14 to 0.72 similarity coefficient index and it was suggestive that grouping of isolates was not related to sampling location in anyway. A high level of genetic heterogeneity of 0.28 was recorded among the isolates.
Bashasab Fakrudin,Vijaykumar Vijaykumar,Kambalapally Krishnareddy Bayyareddy,Kuruvinashetti Mahaling Shrishailappa,Patil Basavaraj Veeranagouda Korean Society of Applied Entomology 2007 Journal of Asia-Pacific Entomology Vol.10 No.1
Morphometric characterization of twelve geographic populations of cotton bollworm, Helicoverpa armigera occurring in south Indian cotton ecosystems was done at larval, pupal and adult stages over three cropping seasons. Traits such as length and weight of larvae, pupa and length and width of the wing, length of fore-, mid- and hind femur, male reproductive organ-length of genital capsule, valves, and ejaculatory duct, female reproductive organ-length of appendix bursae and ductus bursae at adult stage were measured across three years. Populations significantly differed for most of the traits studied. It was evident that populations from northern parts recorded higher phenotypic attributes compared to those from southern parts of south Indian cotton ecosystem. Besides larval, pupal and adult external phenotypic traits, attributes of male reproductive organ viz., length of genital capsule, valves, and ejaculatory duct and female reproductive organ viz., length of appendix bursae and ductus bursae differed significantly among populations. Information on population structure and differences based on morphometry would be useful in better understanding of population dynamics and management of this pest in cotton and other crops in south India.
Suvarna Patil,Fakrudin Bashasab,Vijaykumar,Basavanagoud,Mahaling S Kuruvinashetti,Basavaraj V Patil 한국응용곤충학회 2006 Journal of Asia-Pacific Entomology Vol.9 No.3
The genetic relatedness among Helicoverpa armigera (Hübner) occurring on different host plants prevailing in South India was studied using PCR- RAPD. Genomic DNA was isolated individually from five larvae collected from each of 10 different host plants (except in okra). PCR-RAPD analysis was carried out using a set of 20 random primers which had produced repeatable banding patterns from a original set of 60 primers. A set of 155 amplicon levels were available for analysis, of which 154 were polymorphic. An average of 7.75 bands per primer was recorded. Similarity coefficients based on the frequency of band sharing among host strains varied from 0.25 in cotton and sunflower to 0.72 in groundnut. Clustering analysis on the basis of the PCR-RAPD-generating band sharing indicated that most of the individuals occurring on niger, safflower, green gram, abutilon and lagasca clustered together, indicating greater genetic similarity among themselves, than those occurring on other crops. Furthermore, the pattern of genetic variation in the individuals collected from niger, safflower, green gram, groundnut, abutilon and lagasca was seem to be largely host-dependent.
Fakrudin Bashasab,Vijaykumar,Krishnareddy Bayyareddy Kambalapally,Mahaling Shrishailappa Kuruvinashetti,Basavaraj Veeranagouda Patil 한국응용곤충학회 2007 Journal of Asia-Pacific Entomology Vol.10 No.1
Morphometric characterization of twelve geographic populations of cotton bollworm, Helicoverpa armigera occurring in south Indian cotton ecosystems was done at larval, pupal and adult stages over three cropping seasons. Traits such as length and weight of larvae, pupa and length and width of the wing, length of fore-, mid- and hind femur, male reproductive organ-length of genital capsule, valves, and ejaculatory duct, female reproductive organ-length of appendix bursae and ductus bursae at adult stage were measured across three years. Populations significantly differed for most of the traits studied. It was evident that populations from northern parts recorded higher phenotypic attributes compared to those from southern parts of south Indian cotton ecosystem. Besides larval, pupal and adult external phenotypic traits, attributes of male reproductive organ viz., length of genital capsule, valves, and ejaculatory duct and female reproductive organ viz., length of appendix bursae and ductus bursae differed significantly among populations. Information on population structure and differences based on morphometry would be useful in better understanding of population dynamics and management of this pest in cotton and other crops in south India.
Patil Suvarna,Bashasab Fakrudin,Vijaykumar Vijaykumar,Basavanagoud Basavanagoud,Kuruvinashetti Mahaling S,Patil Basavaraj V Korean Society of Applied Entomology 2006 Journal of Asia-Pacific Entomology Vol.9 No.3
The genetic relatedness among Helicoverpa armigera ($H\"{u}bner$) occurring on different host plants prevailing in South India was studied using PCR-RAPD. Genomic DNA was isolated individually from five larvae collected from each of 10 different host plants (except in okra). PCR-RAPD analysis was carried out using a set of 20 random primers which had produced repeatable banding patterns from a original set of 60 primers. A set of 155 amplicon levels were available for analysis, of which 154 were polymorphic. An average of 7.75 bands per primer was recorded. Similarity coefficients based on the frequency of band sharing among host strains varied from 0.25 in cotton and sunflower to 0.72 in groundnut. Clustering analysis on the basis of the PCR-RAPD-generating band sharing indicated that most of the individuals occurring on niger, safflower, green gram, abutilon and lagasca clustered together, indicating greater genetic similarity among themselves, than those occurring on other crops. Furthermore, the pattern of genetic variation in the individuals collected from niger, safflower, green gram, groundnut, abutilon and lagasca was seem to be largely host-dependent.