ZNF424, a novel human KRAB/C2H2 zinc finger protein, suppresses NFAT and p21 pathway

( Yue Qun Wang ),( Jun Mei Zhou ),( Xiang Li Ye ),( Yong Qi Wan ),( Yong Qing Li ),( Xiao Yan Mo ),( Wu Zhou Yuan ),( Yan Yan ),( Na Luo ),( Ze Qun Wang ),( Xiong Wei Fan ),( Yun Deng ),( Xiu Shan Wu 한국생화학분자생물학회 (구 한국생화학회) 2010 BMB Reports Vol.43 No.3

Zinc finger-containing transcription factors are the largest single family of transcriptional regulators in mammals, which play an essential role in cell differentiation, cell proliferation, apoptosis, and neoplastic transformation. Here we have cloned a novel KRAB-related zinc finger gene, ZNF424, encoding a protein of 555aa. ZNF424 gene consisted of 4 exons and 3 introns, and mapped to chromosome 19p13.3. ZNF424 gene was ubiquitously expressed in human embryo tissues by Northern blot analysis. ZNF424 is conserved across species in evolution. Using a GFP-labeled ZNF424 protein, we demonstrate that ZNF424 localizes mostly in the nucleus. Transcriptional activity assays shows ZNF424 suppresses transcriptional activity of L8G5-luciferase. Overexpression of ZNF424 in HEK- 293 cells inhibited the transcriptional activity of NFAT and p21, which may be silenced by siRNA. The results suggest that ZNF424 protein may act as a transcriptional repressor that suppresses NFAT and p21 pathway to mediate cellular functions. [BMB reports 2010; 43(3): 212-218]

MiR-675 Promotes the Growth of Hepatocellular Carcinoma Cells Through Cdc25A Pathway

Yu, Ya-Qun,Weng, Jun,Li, Shu-Qun,Li, Bo,Lv, Jun Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.8

Background: MicroRNAs (miRNAs) have fundamental roles in tumorigenesis. MiR-675 is upregulated in hepatocellular carcinoma(HCC) cells. However, the roles of miR-675 in hepatocellular carcinogenesis are still not fully elucidated. In this study, we focus on investigating the effect and mechanism of miR-675 in proliferation of HCC cells. Materials and Methods: The cell proliferation was measured by MTT assays after transfection with miR-675 inhibitor and miR-675 mimics in HCC cells. The expression level of miR-675 was detected by real-time quantitative reverse transcription polymerase chain reaction. Protein expression of Cdc25A was measured by western blotting analysis. Results: In MTT assays, overexpression of miR-675 promoted the proliferation of HCC cells(P<0.05. at 48 hours, P<0.01. at 72 hours) compared with the miR-675mimics control group. Downexpression of miR-675 inhibited the proliferation of HCC cells(P<0.05. at 48 hours, P<0.01. at 72 hours) compared with the miR-675inhibitor control group. In western blotting analysis, the expression level of Cdc25A was significantly increased (p<0.05) after treatment with miR-675 mimics. The expression level of Cdc25A was significantly decreased (p<0.05) after treatment with miR-675 inhibitor. Conclusions: Our results indicate that miR-675 promotes the proliferation in human hepatocellular carcinoma cells by associating with Cdc25A signaling pathway.

Maximum Likelihood and Signal-Selective TDOA Estimation for Noncircular Signals

Fei Wen and Qun Wan,Qun Wan 한국통신학회 2013 Journal of communications and networks Vol.15 No.3

This paper addresses the issue of time-difference-ofarrival (TDOA) estimation for complex noncircular signals. First,under the wide-sense stationary assumption, we derive the maximum likelihood (ML) estimator and the Cramer-Rao lower bound for Gaussian noncircular signals in Gaussian circular noise. The ML estimator uses the second-order statistics information of a noncircular signal more comprehensively when compared with the cross-correlation (CC) and the conjugate CC estimators. Further,we present a scheme to modify the traditional signal-selective TDOA methods for noncircular signals on the basis of the cyclostationarity of man-made signals. This scheme simultaneously exploits the information contained in both the cyclic cross-correlation (CCC) and the conjugate CCC of a noncircular signal.

The dietary behavior status of participants in fitness clubs

( Qun Zuo ) 대한운동사협회 2017 대한운동사협회 운동사대회자료집 Vol.2017 No.-

Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Nocardia salmonicida, the Causative Agent of Nocardiosis in Fish

( Qun Xia Li ),( Hong Lian Zhang ),( Yi Shan Lu ),( Jia Cai ),( Bei Wang ),( Ji Chang Jian ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.3

Nocardia salmonicida is one of the main pathogens of fish nocardiosis. The purpose of this study was to build a loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of N. salmonicida. A set of four primers were designed from the 16S-23S rRNA intergenic spacer region of N. salmonicida, and conditions for LAMP were optimized as incubating all the reagents for 60 min at 64°C. LAMP products were judged with agar gel electrophoresis as well as with the naked eye after the addition of SYBR Green I. Results showed the sensitivity of the LAMP assay was 1.68 × 103 CFU/ml (16.8 CFU per reaction) and 10-fold higher than that of PCR. The LAMP method was also effectively applied to detect N. salmonicida in diseased fish samples, and it may potentially facilitate the surveillance and early diagnosis of fish nocardiosis.

Genetic and epigenetic variation in mass selection populations of Pacific oyster Crassostrea gigas

Qun Jiang,Qi Li,Hong Yu,Lingfeng Kong 한국유전학회 2013 Genes & Genomics Vol.35 No.5

Selective breeding often produces an improvementin phenotype. Much of the phenotypic change withina species is a consequence of genetic variation. However,there is growing evidence for phenotypic change even inthe absence of DNA sequence polymorphisms, termedepigenetic variation. This study’s goal was to investigatethe genetic and epigenetic variation in the mass selectionpopulations of the Pacific oyster (Crassostrea gigas),determine if any correlation exists between the genetic andepigenetic variations. This can serve as a first step ininvestigating the potential role epigenetic variations havein selective breeding. Amplified fragment length polymorphismanalysis and methylation-sensitive amplifiedpolymorphism methodology were used to monitor geneticand epigenetic variation in two populations (the base stockand the third selected generation) from a mass selectionline in the Pacific oyster. The correlation between geneticand epigenetic variation was evaluated by Co-InertiaAnalysis. The genetic difference was mainly found in thegene frequency shift revealed by the FST value (0.0151,P\0.01) and no significant reduction in genetic diversitywas detected. The percentage of methylation in C. gigaswas 26.4 %. No significant difference was observed on theaverage state of methylation, but a few bands showed differentfrequencies between the two populations. Co-InertiaAnalysis revealed a significant association between thegenetic and epigenetic profiles (P\0.01). Selective breeding often produces an improvement in phenotype. Much of the phenotypic change within a species is a consequence of genetic variation. However, there is growing evidence for phenotypic change even in the absence of DNA sequence polymorphisms, termed epigenetic variation. This study’s goal was to investigate the genetic and epigenetic variation in the mass selection populations of the Pacific oyster (Crassostrea gigas), determine if any correlation exists between the genetic and epigenetic variations. This can serve as a first step in investigating the potential role epigenetic variations have in selective breeding. Amplified fragment length polymorphism analysis and methylation-sensitive amplified polymorphism methodology were used to monitor genetic and epigenetic variation in two populations (the base stock and the third selected generation) from a mass selection line in the Pacific oyster. The correlation between genetic and epigenetic variation was evaluated by Co-Inertia Analysis. The genetic difference was mainly found in the gene frequency shift revealed by the FST value (0.0151, P\0.01) and no significant reduction in genetic diversity was detected. The percentage of methylation in C. gigas was 26.4 %. No significant difference was observed on the average state of methylation, but a few bands showed different frequencies between the two populations. Co-Inertia Analysis revealed a significant association between the genetic and epigenetic profiles (P\0.01).

Influence of the Interface Property on the Memory Function in a CER Cell Based on Pr0.7Ca0.3MnO3 Films

Qun Wang,김형국,Li Dong Chen,Xiao Min Li,황윤회 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.51 No.2I

Two kinds of colossal electroresistance (CER) cells, Ag/Pr$_{0.7}$Ca$_{0.3}$MnO$_{3}$/Pt and In/Pr$_{0.7}$ Ca$_{0.3}$MnO$_{3}$/Pt, were prepared to study the memory function. Different reversible-resistance-switching phenomena were observed, depending on the applied electrodes. The I-V characteristics of the two cells have opposite hysteresis properties. The power consumptions and the threshold voltages were also investigated. In particular, the Ag/Pr$_{0.7}$Ca$_{0.3}$MnO$_{3}$/Pt cell with an Ohmic contact interface exhibited better resistance switching retention and small power consumption. In contrast, the In/Pr$_{0.7}$Ca$_{0.3}$MnO$_{3}$/Pt cell with a Schottky contact interface exhibited poor resistance switching retention and large power consumption. The results suggest that the CER cell with an Ohmic contact is better for the resistance memory function.

Thermal Characterization of Cardanol as Candidate Resin for Biocomposites

Qun Zhou,Chae Wook Cho,Donghwan Cho,Bong Keun Song 한국고분자학회 2006 한국고분자학회 학술대회 연구논문 초록집 Vol.2006 No.10

Expression and DNA methylation pattern of reproduction-related genes in partially fertile triploid Pacific oysters Crassostrea gigas

Qun Jiang,Qi Li,Hong Yu,Lingfeng Kong 한국유전학회 2017 Genes & Genomics Vol.39 No.9

Partial or complete sterility is an obvious feature in triploid Pacific oyster (Crassostrea gigas) which contributes to improving rearing performances. Despite the significance of sterility, the molecular mechanism behind it remains elusive and related research was limited. This study focused on six reproduction-related genes and compared their different behavior in gene expression and DNA methylation pattern between triploid and diploid oysters in order to provide more molecular information. The gonadal development of triploid oyster was examined by histology before molecular analysis. Gametogenesis disturbance was observed in triploid oysters at different development stages (stage II and III) with more serious impairment in females. QPCR showed significant gene expression difference between diploid and triploid in two genes: putative Vg and cgER. Gene expression of putative Vg was delayed in triploids while for cgER triploid oyster showed higher expression and the difference was significant at stage III. DNA methylation pattern of these two genes were further investigated by bisulfite sequencing. Between diploid and triploid oysters, no difference was observed in total methylation level but some individual loci showed different patterns: significantly high methylation rate of loci 2284 in cgER was observed in triploid oyster which has a higher expression of this gene. This study indicated that putative Vg and cgER might play a role in partial sterile in triploid C. gigas. Gene expression could be regulated by the methylation pattern at specific individual locus, which deserves equivalent attention as well as total DNA methylation level.

중국 네트워크 마케팅 산업에서 제품 애용자가 독립사업자로 활동하려는 의도에 관한 연구: 확장된 계획 행동이론을 중심으로*

Qun Wang,김문홍 한국무역연구원 2023 무역연구 Vol.19 No.5

Purpose – This study aims to investigate the motivations of product enthusiasts in China's network marketing industry to engage in independent business activities, using an extended Theory of Planned Behavior as the theoretical framework. Additionally, it explores the moderating role of prior knowledge of network marketing. Design/Methodology/Approach – By integrating perception as a variable, this research assesses its influence on the entrepreneurial intentions of product loyalists within China's network marketing sector. Furthermore, the study examines how prior knowledge of network marketing affects the relationships among attitude, subjective norms, perceived behavioral control, perception of network marketing, and intent. Findings – The results indicate that variables derived from the extended Theory of Planned Behavior, including attitude, subjective norms, perceived behavioral control, and awareness, positively impact individual intentions to become independent network marketers. Notably, perceived control and awareness also influence actual behavior. Moreover, prior knowledge serves as a moderator for all pathways, except in the relationship between perceived control, awareness, and intention. Research Implications – Network marketing companies should prioritize maintaining high product quality to establish consumer trust. Engaging in corporate social responsibility initiatives can enhance company image. Consistent education for consumers about network marketing can help foster entrepreneurial intentions among potential independent business owners.