Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Nocardia salmonicida, the Causative Agent of Nocardiosis in Fish

( Qun Xia Li ),( Hong Lian Zhang ),( Yi Shan Lu ),( Jia Cai ),( Bei Wang ),( Ji Chang Jian ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.3

Nocardia salmonicida is one of the main pathogens of fish nocardiosis. The purpose of this study was to build a loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of N. salmonicida. A set of four primers were designed from the 16S-23S rRNA intergenic spacer region of N. salmonicida, and conditions for LAMP were optimized as incubating all the reagents for 60 min at 64°C. LAMP products were judged with agar gel electrophoresis as well as with the naked eye after the addition of SYBR Green I. Results showed the sensitivity of the LAMP assay was 1.68 × 103 CFU/ml (16.8 CFU per reaction) and 10-fold higher than that of PCR. The LAMP method was also effectively applied to detect N. salmonicida in diseased fish samples, and it may potentially facilitate the surveillance and early diagnosis of fish nocardiosis.

Curdione Inhibits Proliferation of MCF-7 Cells by Inducing Apoptosis

Li, Juan,Bian, Wei-He,Wan, Juan,Zhou, Jing,Lin, Yan,Wang, Ji-Rong,Wang, Zhao-Xia,Shen, Qun,Wang, Ke-Ming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background: Curdione, one of the major components of Curcuma zedoaria, has been reported to possess various biological activities. It thus might be a candidate anti-flammatory and cancer chemopreventive agent. However, the precise molecular mechanisms of action of curdione on cancer cells are still unclear. In this study, we investigated the effect of curdione on breast cancer. Materials and Methods: Xenograft nude mice were used to detect the effect of curdione on breast cancer in vivo; we also tested the effect of curdione on breast cancer in vitro by MTT, Flow cytometry, JC-I assay, and western blot. Results: Firstly, we found that curdione significantly suppressed tumor growth in a xenograft nude mouse breast tumor model in a dose-dependent manner. In addition, curdione treatment inhibited cell proliferation and induced cell apoptosis. Moreover, after curdione treatment, increase of impaired mitochondrial membrane potential occurred in a concentration dependent manner. Furthermore, the expression of apoptosis-related proteins including cleaved caspase-3, caspase-9 and Bax was increased in curdione treatment groups, while the expression of the anti-apoptotic Bcl-2 was decreased. Inhibitors of caspase-3 were used to confirm that curdione induced apoptosis. Conclusions: Overall, our observations first suggested that curdione inhibited the proliferation of breast cancer cells by inducing apoptosis. These results might provide some molecular basis for the anti-cancer activity of curdione.

Curcumin Analogue A501 induces G2/M Arrest and Apoptosis in Non-small Cell Lung Cancer Cells

Xia, Yi-Qun,Wei, Xiao-Yan,Li, Wu-Lan,Kanchana, Karvannan,Xu, Chao-Chao,Chen, Da-Hui,Chou, Pei-Hong,Jin, Rong,Wu, Jian-Zhang,Liang, Guang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Curcumin and its analogues have been reported to exert anti-cancer activity against a variety of tumors. Here, we reported A501, a new curcumin analogue. The effect of A501 on cell viability was detected by MTT assay, the result showed that A501 had a better inhibiting effect on the four non-small cell lung cancer (NSCLC) cells than that of curcumin. Moreover, Colony forming experiment showed A501 significant restrained cell proliferation. Flow cytometry displayed A501 can cause G2/M arrest and induce apoptosis. Western blotting showed that A501 decreased the expression of cyclinB1, cdc-2, bcl-2, while increased the expression of p53, cleaved caspase-3 and bax. In conclusion, curcumin analogues A501 played antitumor activity by inhibiting cell proliferation and inducing apoptosis of NSCLC cells. And it was likely to be a promising starting point for the development of curcumin-based anticancer drugs.

Preventive Effect of Actinidia Valvata Dunn Extract on N-methyl-N'-nitro-N-nitrosoguanidine-induced Gastrointestinal Cancer in Rats

Wang, Xia,Liu, Hao,Wang, Xin,Zeng, Zhi,Xie, Li-Qun,Sun, Zhi-Guang,Wei, Mu-Xin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Purpose: This study was conducted to assess the preventive effect of Actinidia valvata Dunn (AVD) extract on an animal model of gastrointestinal carcinogenesis on the basis of changes in tumor incidence, cell proliferation, and apoptosis. Materials and Methods: Seventy-five male Wistar rats were divided into five different treatment groups with 15 rats in each group. Group I was given normal feed, whereas Groups II to IV were treated with 10% sodium chloride in the first six weeks and 100ug/mL of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in drinking water for 24 weeks. Group II was then given normal feed, whereas Group III was given AVD extract (0.24g/kg/day) for 12 weeks. Group IV was given AVD extract from the first week to the 36th week, whereas Group V was treated with AVD extract alone for 36 weeks. All rats were sacrificed at the end of the 36-week experiment and assessed for the presence of gastrointestinal tumors. The occurrence of cancer was evaluated by histology. Bax, Bcl-2, Caspase-3, and cyclinD1 were determined by immunohistochemical staining and Western blotting. Results: The incidences of gastric cancer were 0% in Group I, 73.3% in Group II, 33.3% in Group III, 26.7% in Group IV, and 0% in Group V. Bcl-2 and cyclinD1 expression was decreased in AVD extract treated groups, whereas Bax and Caspase-3 expression was increased. Comparison with group II revealed significant differences (p<0.01). Conclusions: AVD extract exhibits an obvious preventive effect on gastrointestinal carcinogenesis induced by MNNG in rats through the regulation of cell proliferation and apoptosis.

Tools Setting of 5 Axis Ultra-Precision Single point Diamond Lathe without OTS

Ke-Tian Li,Xin Chen,Xin-Du Chen,Qiang Liu,Xiang-bo Ouyang,Hong-Jian Xia,Jian-Qun Liu 한국생산제조학회 2014 한국생산제조시스템학회 학술발표대회 논문집 Vol.2014 No.9(2)

The preparation of alginate–AgNPs composite fiber with green approach and its antibacterial activity

Xihui Zhao,Qun Li,Xiaomei Ma,Wanying Liu,Jianping Wang,Yanzhi Xia 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.24 No.-

Antibacterial alginate fibers containing silver nanoparticles (AgNPs) have been prepared in this work. Firstly, AgNPs colloid solution was obtained employing alginate as stabilizing agent and glucose as reducing agent. Then, sodium alginate powder was directly added to the above solution and the resultant sodium alginate–AgNPs spinning dope was spun to prepare alginate–AgNPs composite fibers. The fibers were characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffraction and thermal gravimetric analysis (TG). Mechanical properties of the fibers were also measured. The antimicrobial properties were investigated by Staphylococcus aureus and Escherichia coli, and the results indicated that the composite fibers showed strong antibacterial activity.

Dynamics of fungal community during silage fermentation of elephant grass (Pennisetum purpureum) produced in northern Vietnam

Viet Ha Vu,Xiyang Li,Mengyuan Wang,Rongmei Liu,Guojian Zhang,Wei Liu,Baixue Xia,Qun Sun 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.7

Objective: This study aimed to gain deeper insights into the dynamic changes in spoilage fungi populations during fermentation and the influence of traditional additives on silage quality. Methods: Elephant grass (Pennisetum purpureum) was prepared without any additive (control), and with the addition of 0.5% salt, and 0.5% salt–0.2% sugar mixture. The fungal community was then determined using a classic culturing method and high-throughput sequencing at 0, 5, 15, and 60 days after ensiling. Results: The results showed that the fungal community of elephant grass silage varied significantly between the natural fermentation without any additive and the two additive groups. The diversity and relative abundance of spoilage molds in the control group were much higher than those in the two treatment groups (p<0.05). Three species of yeasts (Candida sp., Pichia sp., Trichosporon sp.) and four spoilage molds (Fusarium sp., Aspergillus sp., Muco sp. and Penicillin sp.) were the predominant fungi in elephant grass during natural fermentation from 0 to 60 days, which were found to be significantly decreased in salt and sugar additive groups (p<0.05). Meanwhile, the diversity and relative abundance of undesirable molds in the 0.5%-salt additive group were the lowest among all groups. Conclusion: Adding salt and sugar, particularly 0.5% salt, is a promising effective approach to reduce the amount of undesirable fungi thus, improving the silage quality of elephant grass in northern Vietnam.

Expression of Ang-2/Tie-2 and PI3K/AKT in Colorectal Cancer

Zhang, Ji-Hong,Wang, Li-Hua,Li, Xiang-Jun,Wang, Ai-Ping,Reng, Li-Qun,Xia, Feng-Guo,Yang, Zhi-Ping,Jiang, Jing,Wang, Xiao-Dan,Wen, Chun-Yang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

Purpose: To study the expression of angiogenin-2 (Ang-2) and its receptor Tie-2 in colorectal cancer and discuss the possible mechanisms behind this process. Materials and Methods: Using the streptavidin-peroxidase (SP) immunohistochemical method, paraffin sections from 100 colorectal cancer samples and 10 samples from tumor-adjacent normal tissue (> 2 cm from the edge of the gross tumor) were tested for protein expression of Ang-2, Tie-2, PI3K, and AKT. Reverse transcription-polymerase chain reaction and Western blots were further used to measure expression of the 4 genes and proteins in 20 freshly-resected colorectal cancer samples and tumor-adjacent normal tissues. Results: In colorectal cancer tissues, the expression of the Ang-2, Tie-2, PI3K, and AKT genes and their proteins was significantly higher than in tumor-adjacent normal tissues. Protein expression in poorly-differentiated adenocarcinoma was higher than that in well and moderately differentiated adenocarcinoma. According to Duke's classification, the protein expression in Stages C and D was significantly higher than that in Stages A and B. In the group with lymphatic metastasis, the protein expression was higher than that without lymphatic metastasis. Conclusions: In colorectal cancer, the expression of the Ang-2, Tie-2, PI3K, and AKT genes and their proteins is markedly higher than those in tumor-adjacent normal tissues. No correlation was observed between protein expression and gender, location, or histologic type. Correlations did exist between protein expression and differentiation level, stage of Duke's classification, and lymphatic metastasis; in colorectal cancer tissues with lower differentiation levels, higher stages of Duke's classification, and lymphatic metastasis, the expression of all 4 proteins was higher. The study of their expression patterns and relationships with aggression and metastasis will provide a valuable experimental foundation for assessing prognosis and targeted therapy of colorectal cancer.

Continuous supply of recycled Pacific oceanic materials in the source of Cenozoic basalts in SE China: the Zhejiang case

Liu, Shao-Chen,Xia, Qun-Ke,Choi, Sung Hi,Deloule, Etienne,Li, Pei,Liu, Jia Springer-Verlag 2016 Contributions to mineralogy and petrology Vol.171 No.12

<P>Various enriched recycled oceanic components in the source of Cenozoic intra-plate alkaline basalts from eastern China were identified by previous studies. Due to the existence of a stagnant subducted Pacific slab in the mantle transition zone beneath eastern China, it is logical to connect the stagnant slab to the recycled oceanic materials. However, the recycled oceanic materials could also result from ancient subduction events (e.g., Paleo-Tethyan, Paleo-Asian or Izanagi plate subduction) because enriched geochemical signatures of a recycled slab can be preserved in the mantle for longer than 1 Gyr. Investigating the temporal variations of the recycled oceanic materials in the mantle source is a useful way to trace the origin of the basalts. In this article, we have conducted a detailed geochemical study, including major and trace elements and Sr-Nd-Pb isotopes, on two alkaline basalt groups from Zhejiang, SE China, which erupted 26-17 Ma and after 11 Ma, respectively. In particular, we recovered the H2O content of the initial magmas based on the H2O content of the clinopyroxene (cpx) phenocrysts and the partition coefficients of H2O between cpx and basaltic melts. The H2O contents of the Zhejiang basalts range from 1.3 to 2.6 (wt.%), which fall within the range of back-arc basin or island arc basalts. The older basalts are more alkaline and have lower Si and Al contents; higher trace element concentrations; higher La/Yb, Ce/Pb and Nb/La ratios; lower H2O/Ce and Ba/Th ratios; and stronger negative K, Pb, Hf and Ti anomalies than the younger ones. The co-relationships between Ba/La, H2O/Ce, Nb/La, Ce/Pb and Ba/Th in the two groups of the Zhejiang basalts indicate that a recycled dehydrated oceanic alkaline basalt component is needed in the source of the older rocks, along with a depleted mantle component. Meanwhile, an additional recycled dehydrated sediment component was required in the source of the younger rocks. The temporal change in the recycled oceanic materials in the mantle sources of Zhejiang Cenozoic basalts demonstrates that the recycled components can only originate in the stagnant Pacific slab that is the only plate subducted since 100 Ma in this area.</P>