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Liu Yang,Ce Shi,Xiaoying Mu,Chao Liu,Ke Shi,Wen-jiao Zhu,Qing Yang 한국식물생명공학회 2015 Plant biotechnology reports Vol.9 No.4
Plant P450s play an important role in plant response to environmental stimuli, especially to pathogen challenge, besides leading to the biosynthesis of secondary metabolites. In this work, a Verticillium dahliae-induced cDNA sequence was cloned from wild eggplant Solanum torvum and designated StoCYP77A2. StoCYP77A2 encodes a 511-amino acid peptide that shows sequence homology to the plant CYP77 family. StoCYP77A2 is expressed in a tissue-specific manner with the highest expression level in stem. The expression level of StoCYP77A2 was increased in wild eggplants which were infected with Verticillium dahlia. We expressed StoCYP77A2 in V. dahliae-sensitive tobacco, and the transgenic tobacco plants were more resistant to V. dahliae infection than the wild type. Protein extraction of transgenic tobacco showed strong antifungal activity against V. dahliae, indicating that StoCYP77A2 may be involved in the synthesis of some antimicrobial compounds. These results suggest that StoCYP77A2 plays a role in plant defense to V. dahliae infection.
Qing-yan Liu,Fang Yang,Zhi-hua Liu,Gang Li 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.26 No.-
The process of corncob hydrolysis to furfural in this paper included the saccharification of hemicellulose to monosaccharide, isomerization of xylose to xylulose and dehydration of xylulose to furfural. The SnO2–Co3O4/C biochar catalyst was prepared by containing sugar solution and lignocelluloses residue from corncob degradation, as well as the mixture of Sn(IV) and Co(II) hydroxide. The maximum yield of furfural have been achieved as 30.0% with 0.3 g SnO2–Co3O4–2/C catalyst for catalytic hydrolysis at 180 8C for duration 200 min. Xylose isomerization using a solid Lewis acid catalyst in water medium is a key step for increasing furfural production from corncob hydrolysis.
Overview of aristolochic acid nephropathy: an update
Zhou Qing-Qing,Jiang Lei,Tao Su,Gang Liu,Yang Liu 대한신장학회 2023 Kidney Research and Clinical Practice Vol.42 No.5
Aristolochic acid nephropathy (AAN) is a rapidly progressive renal interstitial fibrosis caused by medical or environmental exposure to aristolochic acid (AA). Since the outbreak of AAN in Belgium was reported nearly 30 years ago, the safety of herbal remedies has drawn considerable attention, and AAN has become a global public health problem. Breakthroughs have been made to better understand the disease, including the toxicity of AAs, the possible mechanisms of AAN, the disease patterns, and the pathological features; however, some critical problems remain unresolved. Because of the insidious onset of the disease, the incidence of AAN and the prevalence of exposure to AAs are unknown and might be largely underestimated. During the past decades, AA-containing herbs have been strictly administrated in many regions and the occurrence of AAN has declined sharply, yet cases of AAN are still sporadically reported. Despite the progress in the understanding of the disease’s pathogenesis, there is no effective treatment for delaying or reversing the renal deterioration caused by AAN. Therefore, the risk of exposure to AAs should be taken seriously by public health workers and clinicians. In this review, we updated the latest data on AAN, summarized the advances throughout these years, and put forward some challenges for future research.
Research on Aggregation and Propagation of Self-Similar Traffic in Satellite Network
Zhenyu Na,Yi Liu,Yang Cui,Qing Guo 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.1
It has already been confirmed that the traffic in high-speed terrestrial network presents self-similarity, but there is little research on self-similarity of traffic in satellite network. Considering time-varying network topology and link status, this paper analyzes the aggregation and propagation of self-similar traffic between nodes in satellite network. Furthermore, a sort of special network node called ground gateway is modeled, based on which the characteristics of the output traffic that the input traffic from terrestrial network passes gateway into satellite network are analyzed. Theoretically analyses demonstrate that after aggregation and propagation between satellite nodes, traffic is still self-similar, and the self-similarity of the output traffic generated by gateway from terrestrial network to satellite network is more often than not weakened.
Photoactivation and inactivation of <i>Arabidopsis</i> cryptochrome 2
Wang, Qin,Zuo, Zecheng,Wang, Xu,Gu, Lianfeng,Yoshizumi, Takeshi,Yang, Zhaohe,Yang, Liang,Liu, Qing,Liu, Wei,Han, Yun-Jeong,Kim, Jeong-Il,Liu, Bin,Wohlschlegel, James A.,Matsui, Minami,Oka, Yoshito,Lin American Association for the Advancement of Scienc 2016 Science Vol.354 No.6310
<P>Cryptochromes are blue-light receptors that regulate development and the circadian clock in plants and animals. We found that Arabidopsis cryptochrome 2 (CRY2) undergoes blue light-dependent homodimerization to become physiologically active. We identified BIC1 (blue-light inhibitor of cryptochromes 1) as an inhibitor of plant cryptochromes that binds to CRY2 to suppress the blue light-dependent dimerization, photobody formation, phosphorylation, degradation, and physiological activities of CRY2. We hypothesize that regulated dimerization governs homeostasis of the active cryptochromes in plants and other evolutionary lineages.</P>
NPC-IGCT Phase Module Clamp Circuit LRC Parameters Design considering FRD snappy Recovery
Yang Ju Zou,Jia Xi Hu,Zhen Yu Ma,Jian Ping Liu,Run Qing Guo,Zhi Xue Zhang 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5
In this paper, a method for neutral point clamping-integrated gate commutated thyristor (NPCIGCT) phase module clamp circuit parameters design considering FRD snappy recovery is present. Based on the snappy recovery theory, and analysis of snappy recovery factors, the paper has shown decreasing current commutating slope can attenuated snappy recovery affect effectively. Then, the paper has shown that it is reasonable for decreasing di/dt by increasing inductance of NPC-IGCT phase module clamp circuit, based on the circuit working principle. Then, the new clamp circuit parameters design method which combine multi-objective optimization solution mathematical module of the circuit, fast recovery diode (FRD) snappy recovery, devices overvoltage and loss is shown in paper. A design example and its test results have demonstrated both IGCT and FRD characteristics have been guaranteed and ensuring safety and reliability of the NPC - IGCT phase module.
Liu, Qing,Sun, Shujing,Piao, Meizi,Yang, Ji Young The Korean Society of Food Science and Nutrition 2013 Preventive Nutrition and Food Science Vol.18 No.4
Protease widely exists in the digestive tract of animals and humans, playing a very important role in protein digestion and absorption. In this study, a high protease-producing strain Planomicrobium sp. L-2 was isolated and identified from the digestive tract of Octopus variabilis. The strain was identified by physiological and biochemical experiments and 16S rDNA sequences analysis. A protease was obtained from the strain Planomicrobium sp. L-2 through ammonium sulfate precipitation, dialysis and enrichment, DEAE-Sephadex A50 anion-exchange chromatography, and Sephadex G-100 gel chromatography. The molecular weight and properties of the protease were characterized, including optimum temperature and pH, thermal stability, protease inhibitions and metal ions. According to our results, the protease from Planomicrobium sp. L-2 strain designated as F1-1 was obtained by three-step separation and purification from crude enzyme. The molecular weight of the protease was 61.4 kDa and its optimum temperature was $40^{\circ}C$. The protease F1-1 showed a broad pH profile for casein hydrolysis between 5.0~11.0. No residual activity was observed after incubation for 40 min at $60^{\circ}C$ and 60 min at $50^{\circ}C$. F1-1 protease was inhibited by $Mn^{2+}$, $Hg^{2+}$, $Pb^{2+}$, $Zn^{2+}$, and $Cu^{2+}$ ions, as well as PMSF, indicating that the protease F1-1 was a serine protease. Additionally, research basis provided by this study could be considered for industrial application of octopus intestinal proteases.