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Pushparaj Karthika,Chithravel Vadivalagan,Natraj Krishnaveni,Kadarkarai MURUGAN,Marcello Nicoletti,Angelo Canale,Giovanni Benelli 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1
Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae) is one of the most important pests attacking Solanaceae crops in Asian countries. Despite its agricultural importance, little information is available on the molecular ecology and evolution. In this study, we used DNA barcoding to explore the genetic diversity and intrapopulation polymorphisms of H. vigintioctopunctata. Haplotype analysis of the partial COI gene sequences of H. vigintioctopunctata fromfour localities in South India and other globally available haplotypes revealed two genetically distinctive lineages. The TCS haplotype network with the main clusters G1, G2, G4 and G6 represented intra-population polymorphismamong the Indian lineages,while other clusterswere differentiatedwith unique mutational steps. High substitutions in the 1st and 3rd codon suggested the dominance of synonymous substitutionswith significant p-values b 0.05, that inferred for purifying selection. The genetic diversity indices Fu's Fs and neutrality index Tajima-D had negative values that implied for recent population expansion and lowfrequency of polymorphism. Further, high genetic divergences among certain haplotype comparisons evidenced the influence of micro-evolutionary pressures. The threshold value was 1.26%, which was supported by the postulates of 10× rule. Overall, the study firstly reported the genetic diversity and intra-population polymorphism of H. vigintioctopunctata populations attacking Solanaceae species in Asian countries.
Feature Selection for Intrusion Detection System in Internet-of-Things (IoT)
Pushparaj Nimbalkar,Deepak Kshirsagar 한국통신학회 2021 ICT Express Vol.7 No.2
Internet of Things (IoT) is suffered from different types of attacks due to vulnerability present in devices. Due to many IoT network traffic features, the machine learning models take time to detect attacks. This paper proposes a feature selection for intrusion detection systems (IDSs) using Information Gain (IG) and Gain Ratio (GR) with the ranked top 50% features for the detection of DoS and DDoS attacks. The proposed system obtains feature subsets using insertion and union operations on subsets obtained by the ranked top 50% IG and GR features. The proposed method is evaluated and validated on IoT-BoT and KDD Cup 1999 datasets, respectively, with a JRipclassifier. The system provides higher performance than the original feature set and traditional IDSs on IoT-BoT and KDD Cup 1999 datasets using 16 and 19 features, respectively.
Two- and Three-Input Fuzzy PID Controller Structure of Takagi-Sugeno Type
Ritu Raj,Pushparaj Mani Pathak,Garima Bhandari,Jung-Min Yang 제어로봇시스템학회 2019 제어로봇시스템학회 국제학술대회 논문집 Vol.2019 No.10
In this paper, we analyze and present a general structure of fuzzy PID controller of Takagi-Sugeno (TS) type having two and three inputs. We show that while three-input fuzzy PID controller gains have an one-to-one relation with the conventional controller gains, this relationship is not directly evident in the case of two-input fuzzy PID controllers. The tuning of input/output scaling parameters of the presented fuzzy PID controllers is addressed based on the structure of conventional PID controllers.
Biological Constraints in Algal Biotechnology
Torzillo, Giuseppe,Pushparaj, Benjamin,Masojidek, Jiri,Vonshak, Avigad The Korean Society for Biotechnology and Bioengine 2003 Biotechnology and Bioprocess Engineering Vol.8 No.6
In the past decade, considerable progress has been made in developing the appropriate biotechnology for microalgal mass cultivation aimed at establishing a new agro-industry. This review points out the main biological constraints affecting algal biotechnology outdoors and the requirements for making this biotechnology economically viable. One of them is the availability of a wide variety of algal species and improved strains that favorably respond to varying environmental conditions existing outdoors. It is thus just a matter of time and effort before a new methodology like genetic engineering can and will be applied in this field as well. The study of stress physiology and adaptation of microalgae has also an important application in further development of the biotechnology for mass culturing of microalgae. In outdoor cultures, cells are exposed to severe changes in light and temperature much faster than the time scale re-quired for the cells to acclimate. A better understanding of those parameters and the ability to rapidly monitor those conditions will provide the growers with a better knowledge on how to optimize growth and productivity. Induction of accumulation of high value products is associated with stress conditions. Understanding the physiological response may help in providing a better production system for the desired product and, at a later stage, give an insight of the potential for genetic modification of desired strains. The potential use of microalgae as part of a biological system for bioremediation/detoxification and wastewater treatment is also associated with growing the cells under stress conditions. Important developments in monitoring and feedback control of the culture behavior through application of on-line chlorophyll fluorescence technique are in progress. Understanding the process associated with those unique environmental conditions may help in choosing the right culture conditions as well as selecting strains in order to improve the efficiency of the biological process.
Solvent free synthesis of coumarin derivative by the use of AlSBA-1 molecular sieves
Mei Mei Peng,장현태,Pushparaj Hemalatha,Mani Ganesh,Muthiahpillai Palanichamy 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.3
AlSBA-1 molecular sieves were synthesized successfully and used for the synthesis of coumarins throughPechmann condensation. The synthesized AlSBA-1 catalysts were characterized by XRD, BET, SEM, FTIR,TGA techniques. The synthesized materials possess large surface area with mesopores. Pechmanncondensation of 3-methoxyphenol and ethyl acetoacetate was carried out under solvent free conditionand 7-methoxy-4-methylcoumarin was the main product. The selectivity of the product was found to be74.04%. Among the synthesized catalysts, AlSBA-1(2) showed high activity than others due to highdensity of acid sites. Hence, AlSBA-1 molecular sieves a better choice of catalyst for the synthesis ofcoumarins.
Ion Pair Colorimetric Estimation of Dronedrone HCl in Solid Dosages Using Methyl Orange
Ganesh, Mani,Hemalatha, Pushparaj,Peng, Mei Mei,Vinodh, Rajangam,Sakthimanigandan, Kalaimani,Jang, Hyun Tae Asian Journal of Chemistry 2014 Asian Journal of Chemistry Vol.26 No.2
<P>New ion pair colorimetric method has been developed for the estimation of dronedrone in bulk and in tablet formulation using methyl orange dye as an ion paring complexant using pH 4 buffer. The developed method h validated for its sensitivity and specificity. The intensity of ion pair complex Of dronedrone and methyl orange found linear over the range of 2-20 mu g mL(-1) with a correlation coefficient of 0.9958. The inter and intraday precision, ace-tiny, stability LOD, LOQ placebo interference, robustness and ruggedness Were evaluated during validation The % RSD found for the recovery of the method resulted with low value of about 0.72-0.99 and with good precision. From the results obtained the developed method was found suit:able and comparable in terms of estimating dronedrone in formulation With that of existing methods.</P>