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Anhai Chen,Chufeng He,Yong Feng,Jie Ling,Xin Peng,Xianlin Liu,Shuang Mao,Yongjia Chen,Mengyao Qin,Shuai Zhang,Yijiang Bai,Jian Song,Zhili Feng,Lu Ma,Dinghua He,Lingyun Mei1 대한이비인후과학회 2023 Clinical and Experimental Otorhinolaryngology Vol.16 No.4
Objectives. Branchio-oto syndrome (BOS) primarily manifests as hearing loss, preauricular pits, and branchial defects. EYA1is the most common pathogenic gene, and splicing mutations account for a substantial proportion of cases. However,few studies have addressed the structural changes in the protein caused by splicing mutations and potential pathogenicfactors, and several studies have shown that middle-ear surgery has limited effectiveness in improving hearing in thesepatients. BOS has also been relatively infrequently reported in the Chinese population. This study explored the ge-netic etiology in the family of a proband with BOS and provided clinical treatment to improve the patient’s hearing. Methods. We collected detailed clinical features and peripheral blood samples from the patients and unaffected individualswithin the family. Pathogenic mutations were identified by whole-exome sequencing and cosegregation analysis andclassified according to the American College of Medical Genetics and Genomics guidelines. Alternative splicing wasverified through a minigene assay. The predicted three-dimensional protein structure and biochemical experimentswere used to investigate the pathogenicity of the mutation. The proband underwent middle-ear surgery and was fol-lowed up at 1 month and 6 months postoperatively to monitor auditory improvement. Results. A novel heterozygous EYA1 splicing variant (c.1050+4 A >C) was identified and classified as pathogenic (PVS1(RNA),PM2, PP1). Skipping of exon 11 of the EYA1 pre-mRNA was confirmed using a minigene assay. This mutation mayimpair EYA1-SIX1 interactions, as shown by an immunoprecipitation assay. The EYA1-Mut protein exhibited cellularmislocalization and decreased protein expression in cytological experiments. Middle-ear surgery significantly improvedhearing loss caused by bone-conduction abnormalities in the proband. Conclusion. We reported a novel splicing variant of EYA1 in a Chinese family with BOS and revealed the potential molec-ular pathogenic mechanism. The significant hearing improvement observed in the proband after middle-ear surgeryprovides a reference for auditory rehabilitation in similar patients.
Oxidation of Ethylbenzene Using Nickel Oxide Supported Metal Organic Framework Catalyst
Peng, Mei Mei,Jeon, Ung Jin,Ganesh, Mani,Aziz, Abidov,Vinodh, Rajangam,Palanichamy, Muthiahpillai,Jang, Hyun Tae Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.11
A metal organic framework-supported Nickel nanoparticle (Ni-MOF-5) was successfully synthesized using a simple impregnation method. The obtained solid acid catalyst was characterized by Powder X-ray diffraction (XRD), scanning electron microscopy (SEM), nitrogen adsorption-desorption and thermogravimetric analysis (TGA). The catalyst was highly crystalline with good thermodynamic stability (up to $400^{\circ}C$) and high surface area ($699m^2g^{-1}$). The catalyst was studied for the oxidation of ethyl benzene, and the results were monitored via gas chromatography (GC) and found that the Ni-MOF-5 catalyst was highly effective for ethyl benzene oxidation. The conversion of ethyl benzene and the selectivity for acetophenone were 55.3% and 90.2%, respectively.
Solvent free synthesis of coumarin derivative by the use of AlSBA-1 molecular sieves
Mei Mei Peng,장현태,Pushparaj Hemalatha,Mani Ganesh,Muthiahpillai Palanichamy 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.3
AlSBA-1 molecular sieves were synthesized successfully and used for the synthesis of coumarins throughPechmann condensation. The synthesized AlSBA-1 catalysts were characterized by XRD, BET, SEM, FTIR,TGA techniques. The synthesized materials possess large surface area with mesopores. Pechmanncondensation of 3-methoxyphenol and ethyl acetoacetate was carried out under solvent free conditionand 7-methoxy-4-methylcoumarin was the main product. The selectivity of the product was found to be74.04%. Among the synthesized catalysts, AlSBA-1(2) showed high activity than others due to highdensity of acid sites. Hence, AlSBA-1 molecular sieves a better choice of catalyst for the synthesis ofcoumarins.
Oxidation of Ethylbenzene Using Nickel Oxide Supported Metal Organic Framework Catalyst
Mei Mei Peng,Ung Jin Jeon,Mani Ganesh,Abidov Aziz,RAJANGAMVINODH,Muthiahpillai Palanichamy,장현태 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.11
A metal organic framework-supported Nickel nanoparticle (Ni–MOF–5) was successfully synthesized using a simple impregnation method. The obtained solid acid catalyst was characterized by Powder X–ray diffraction (XRD), scanning electron microscopy (SEM), nitrogen adsorption–desorption and thermogravimetric analysis (TGA). The catalyst was highly crystalline with good thermodynamic stability (up to 400 °C) and high surface area (699 m2g–1). The catalyst was studied for the oxidation of ethyl benzene, and the results were monitored via gas chromatography (GC) and found that the Ni–MOF–5 catalyst was highly effective for ethyl benzene oxidation. The conversion of ethyl benzene and the selectivity for acetophenone were 55.3% and 90.2%, respectively.
Selective Oxidation of Ethylbenzene over Cobalt Impregnated Zeolite ZSM-5
Mei Mei Peng(펭메이메이),Mani Ganesh(마니 가니쉬),Abidov Aziz(아비도프 아지즈),Ung Jin Jeon(전웅진),Rajangam Vinodh(라자감 비노드),Muthiahpillai Palanichamy(무따이 팔라리차미),Hyun Tae Jang(장현태) 한국산학기술학회 2014 한국산학기술학회 학술대회 Vol.- No.-
A zeolite supported cobalt catalyst Co-ZSM-5 was synthesized and characterized by Powder X-ray diffraction (XRD), scanning electron microscopy (SEM), nitrogen adsorption-desorption and thermogravimetric analysis (TGA). The synthesized catalyst was used for ethylbenzene oxidation reaction. The catalytic reaction was carried out in liquid phase using oxygen as oxidizing agent in solvent-free condition. The results analyzed by Gas chromatography (GC) displayed the catalyst was effective for ethyl benzene oxidation.
팽메이메이(Peng, Mei Mei),차왕석(Cha, Wang-Seog) 한국산학기술학회 2013 한국산학기술학회논문지 Vol.14 No.10
methylethylketone(MEK)의 광분해 특성을 연속식 단일관형태의 광촉매반응기를 이용하여 연구하였다. 연속식 광촉매반응기에 적용된 주요 반응변수는 MEK의 초기농도, 광세기, 장기운전의 영향이었다. MEK의 주입농도가 증가 할수록 광분해 효율은 감소하였으며, 광분해 감소의 폭은 높은 유속에서 더욱 두드러졌다. 동일한 체류시간의 조건에 서 광반응기의 직경과 UV램프의 파장이 증가할수록 MEK제거효율은 감소하였다. 연속식 광촉매반응기를 120시간 연 속적으로 운전하여도 MEK 제거효율에서의 감소는 없었다. The degradation of methylethylkeone(MEK) was investigated by the continuous single module photocatalytic reactor. Operational conditions were initial concentration of MEK, intensity of photon flux, and activity change according to the long time operation. The photocatalytic degradation was decreased with the increase of MEK concentration, and the degree of decrease was larger at higher flow rate. Removal efficiency of photocatalytic reactor was decreased with the increase of reactor diameter and lamp wavelength under the same residence time condition. Continuous single module photocatalytic reactor was successfully operated without any activity drop during 120hrs operation.
Mei-Fang Chen,Yuan Yuan Zhang,Mei Di He,Cheng Yong Li,Chun Xia Zhou,Peng Zhi Hong,Zhong-Ji Qian 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.2
Marine microalgae have been widely applied in cosmaceuticals, nutraceuticals, and functional foods. In the present study, we first investigated the hepatoprotective effects of peptide purified from microalgae, Isochrysis zhanjiangensis on HepG2 cells alcoholic injury. I. zhanjiangensis was hydrolyzed utilizing chymotrypsin, trypsin, pepsin, and by vitro gastrointestinal digestion. Among hydrolysates, the gastrointestinal hydrolysate showed relatively high free radical scavenging ability preliminarily and was purified with following sequential chromatography methods. The amino acid sequence and molecular mass of the purified peptide from I. zhanjiangensis (PIZ) was identified as Asn-Asp-Ala-Glu-Tyr-Gly-Ile-Cys-Gly-Phe (NDAEYGICGF; MW, 1088.16 Da) via Q-TOF ESI/MS. Additionally, PIZ attenuated ethanol-induced cytotoxicity and inhibited the production of intracellular reactive oxygen species by DCFH-DA assay. Western blot results showed that superoxide dismutase (SOD) and glutathione (GSH) levels up-regulated with PIZ treatment before alcohol exposure while gamma-glutamyltransferase (GGT) protein expression down-regulated. These results provide an opportunity to discover new highly active peptide against alcohol toxicity in HepG2 cells.