cAMP가 보리와 귀리잎 Protoplast의 팽윤에 미치는 영향

송필순,송성준,유장걸,문두길 제주대학교 방사능이용연구소 1988 연구보고 Vol.3 No.-

암조건에서 생육시킨 보리(barley)와 귀리(oat) 잎의 상단부에서 분리한 protoplasts는 dibutyryl cAMP 처리에 의해서 팽윤되었으며 또한, 적색광과 cAMP 혼합처리에 의해서는 protoplasts 팽윤효과가 상승되었다. 따라서 phytochrome작용계와 cAMP는 일련의 공통기능을 갖고 있는 것으로 사료되며, GA₃처리에 의해서도 보리와 귀리의 protoplasts가 팽윤되므로 GA₃와 cAMP 그리고 phytochrome작용계가 서로 연류되어 있을 가능성이 있다고 생각된다. 한편, 적색광을 조사하거나 GA₃를 처리한후 전자현미경사진을 촬영해 봤을 때, 적색광처리에서는 plastid발달이, GA₃처리에서는 액포발달이 현저하였다. The effect of cAMP on the swelling of protoplasts isolated from the primary leaves of etiolated barley and oat was studied. The presence of 50ul dibutyryl cAMP caused protoplast swelling. The red light irradiation after adding dibutyryl cAMP enhanced the increase of protoplast size. It is likely that cAMP and phytochrome have common regulatory mechanism mediating protoplast swelling. The protoplast swelling of oat and barley leaves was accelerated by GA₃addition, indicating that cAMP, phytochrome and GA₃were closely involved in protoplast swelling. The electron microscopic pictures showed that red light irradiation made better plastid development in oat protoplasts and GA₃addition increased the vacuole size.

화학광량계에 의한 Stentor coeruleus 중의 ATP 정량

민태진,송필순 동국대학교 자연과학연구소 1984 자연과학연구 논문집 Vol.4 No.-

S. coeruleus의 소광반응 촉진에 관계하는 적색광을 조사하여, 세포내 ATP량의 변화를 관찰하였다. luciferase-luciferin 반응의 생물발광을 좌우하는 ATP를 화학광량계로 Potassium ferrioxalate를 사용하는 방법에 의하여 측정하였다. S. coeruleus에 610nm의 빛을 2초동안 조사함에 따라, 세포내 ATP농도는 대조구에 비하여 총단백질 1,000ug당 710nM이 증가되었다. 2초이상 (45초까지) 조사할때는 ATP량이 점점 감소하였지만, 45초 이상 조사시는 ATP농도가 점점 증가하여, 120초 조사에서는 2초동안 조사하였을때의 농도와 유사하였다. 이런 결과는 S. coeruleus 내 Ca?-ATPase의 역활에 기인되는 것으로 보여진다. Changes in cellular ATP level due to red light irradiation and its possible correlation to the step-up photophobic response in S. coeruleus have been investigated. The ATP dependent bioluminescence of luciferase-luciferin reaction was measured by the potassium ferrioxalate chemical actinometric method. Upon irradiation of S. coeruleus with 610nm actinic light for 2 seconds, the ATP concentration in the cells increased by 71nmole/1,000ug of total protein as compared to that of the control group. Irradiation beyond 2 seconds (up to 45 seconds) resulted in a decrease in the ATP level. However, subsequent irradiation caused a gradual increase in the ATP concertration which reached a preirradiation level at 120 seconds of irradiation. These results reflect as a functional rele of Ca?-ATPase in S. coeruleus.

Tomatotone과 Gibberellin(GA3)의 處理가 輸出用 가지(Solanum melongena. L)의 生育 및 收量에 미치는 影響

최성관,이재필,진동호,오주성,박흥식,정순재 동아대학교 농업생명과학연구소 2001 農業生命資援硏究 Vol.10 No.1

These grafting seeding "Torobambica", rootstock "Jang Ja" and "Chuk Yang" were tested to investigate the differences between the growth of eggplants and the number of eggplants which can be influenced by the growth retardant aerosol. For the test of the growth retardant aerosol, the flower clusters were sprayed with one hundred units of Tomatotone liquid and with three hundred units of GA3 on the day they flowered and sprayed two or three days before flowering. The results were as follows: 1.According to the growth retardant aerosol of Tomatotone, the plant height was the tallest at 226.21 cm with foliar spray and the high Marketable fruit was good at the percent of 77.38 with a flowering spray and Tomatotone on the day they flowered. 2.According to the growth retardant aerosol of GA3, the plant's height was the tallest at 229.27cm with foliage which was sprayed every week. The high quality fruit was good at the percent of 69.11 with a flowering spray and GA3 on the day they flowered. 3.According to Tomatotone and GA3 treatments of the plant growth regulators, the plant's height was the tallest sprayed every week and the high Marketable fruit was good at the flowering spray and Tomatotone on the day they flowered. and Yield per 10a was good at the flowering spray and Tomatotone on the day they flowered.

Phytochrome이 보리잎 Protoplast의 팽윤에 미치는 영향

홍경애,송성준,송필순,유장걸,문두길 제주대학교 방사능이용연구소 1986 연구보고 Vol.2 No.-

암조건에서 생육시킨 보리(Hordeum valgare)잎의 생장점 부근에서 분리한 Protoplast가 적색광 처리에 의해서 팽윤되었고 적외선광에 의해서는 적색광 효과과 소멸되었다. 이 사실은 Protoplast의 팽윤이 Phytochrome에 의해서 조절됨을 시사하는 것이나 Phytochrome의 작용기작을 알기 위해서는 다각적인 연구가 필요하다. 실험결과로 부터 적색광 처리시간은 2분 적색광 처리후 배양시간은 2시간이 적당함을 알았다. Protoplast 배양액의 Sorbitol 농도에 따른 크기변화는 농도가 낮을수록 커졌으며 0.4M 이하에서는 Protoplast가 파괴되었다. Light effect on the size of protoplasts isolated from etiolated barley leaves was studied. Red light irradiation caused a swelling of protoplasts while little change in size found under the dark condition. Photoreversibility by red light and far-red light was found in protoplast swelling, indicating the involvement of phytochrome. Action mechanism of phytochrome on protoplast swelling should be clarified in aspect of growth regulators and intermediates involved. Two minute irradiation time and two hour incubation after red light treatment were found sufficient to induce protoplast swelling. The protoplasts were swollen according to the sorbitol concentration of incubation media but destroyed below 0.4M-sorbitol concentration.

Goretex를 이용한 융비술

최진영,민철기,명훈,황순정,서병무,이종호,정필순,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.4

융비술시 실리콘 고무는 다루기 쉽고 조직 반응이 적어 현대 많이 돌출의 합병증이 생길 수 있다. 저자등은 이러한 실리콘의 단점을 보완할 수 있는 goretex를 사용하여 융비술을 시행하고 비첨에 대해서 자가연골을 이식하여 만족할 만한 결과를 얻었기에 goretex가 안면부 연조직의 증강을 위한 이식재료로 적합하다고 생각되어 문헌고찰과 함께 보고하는 바이다. Many different materials have been used to provide augmentation of the nasal dorsum. this include both autogenous and homogenous materials,as well as allografts. Silicone among the artificial materials has been most widely used. The silicone has some advantages and disadvantages. Goretex(expanded polytetra-fluoroethylene) which has widely used in vascular surgery and abdominal surgery can be used for facial soft tissue augmentation. The author presemts 2 cases of nasal augmentaion using goretex with literature review.

단세포 나팔벌레,Stentor coeruleus의 빛에 의한 ATP 합성

Chen, B.H.,허재욱,민태진,Iwatsuki, K,송필순 동국대학교 자연과학연구소 1986 자연과학연구 논문집 Vol.6 No.-

나팔벌레의 세포내 ATP 함량은 0.5~2초동안 40W/m₂백색광을 조사함에 따라 어두운 상태의 대조구에 비하여 급격히 증가하였고, 빛을 계속하여 조사하면 세포내 ATP 함량은 단계적으로 감소하였다. 광수용체 색소 Stentorin 을 포함하고 있는 색소 미립자를 나팔벌레 완전세포로부터 분리하여 이에 ADP와 pi를 가한 혼합물에 빛을 조사하여 ATP가 생성됨을 확인하였다. 나팔벌레 완전세포와 색소미립자주으이 ATP 함량은 변량울에 (fluence rate)의존하였다. 이들 결과로 생명체는 광양자를 ATP로 전이시킬 수 있고, ATP 생성은 나팔벌레 색소미립자의 특성으로 사려된다. The cellular ATP level in Stentor coeruleus jumped above the dark control level when stimulated by 0.5-2 second light pulses (40W/m₂white light). Continued, irradiation fo the cells caused a gradual decline of the cellular ATP level. Pigment granules containing the photoreceptor pigment stentorin isolated from whole cells also exhibited a light-induced ATP synthesis. The ATP level in both whole cells and pigment granules was dependent upon the fluence rate. These results suggest that the organism is capable of tranducing light quanta into ATP and that ATP synthesis is localized in the pigment granule.

하치조신경 및 설신경 손상 평가를 위한 한국인 정상 성인의 하순-이부 및 혀의 감각 조사

이종호,이세영,송승일,이은진,안강민,김성민,명훈,황순정,서병무,최진영,정필훈,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.2

In the head and neck area, there are so many sensory nerves, which are sometimes injuried iatrogenically or inadvertently so that involved patients complained of the loss of sensations. In such cases, it is important to judge the degree of injuries and regeneration of nerve for better diagnosis and treatment. Seddon and Sunderland's classification, which is commomly used, is focused on histological change and nerve conduction. As times goes by, it is difficult to access patient's sensory disturbance by this method. Until now, so many methods such as contract threshold, direction, two-point discrimination, pin prick, thermal discrimination and current perception threshold have been introduced for sensory evaluation. However, there hasn't been enough information regarding each methodology nor integrated standard methodology for the measurement. the purpose of this study is to get Korean adult normative sensory values of lower lip,chin and tongue using modified methods of contact thershold, ditection, two point discrimination, pin prick, thermal discrimination and assess degree of regeneration of sensory nerve damage.

비복신경이식을 이용한 긴 거리의 말초신경 결손부 수복

이종호,이세영,김명진,이은진,안강민,김성민,최원재,명훈,황순정,서병무,최진영,정필훈 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.2

The surgery of oral and maxillofacial area poses the risk of cranial nerve damage such as trigeminal nerve or facial nerve. Inferior alveolar nerve is prone to damage in the third molar extraction, implant installation, orthognathic surgery, open reduction and rigid fixation, and tumor ablation surgery. On the other hands,facial nerve is likely to be damaged or sacrificed with trauma or parotidectomy. In case of inferior alveolar nerve injury, the incidence is reported to be about 1.3%. The nerve function will almost recover in minimal damage, but it won't recover at last in total damage of a part of nerve unit. In latter cases, nerve regeneration in intended by allograft as nerve substitute or various route of merve condit. But the recovery with autograft is believed to be most relialbe mrthod in the rapair of long-span(longer than 15㎜)nerve defect. We have performed autologous sural nerve graft in the repair of nerve defect, which is caused by resection of benign or malignant tumor. Hereby we report the method of nerve harvesting, recovery of defected peripheral nerve and the complications of donor site with the discussion of sural nerve anatomy.

A Molecular Model for the Structure and Function of phytochrome in Higher Plants

( Pill Soon Song,In Soo Kim,Tae Ryong Hahn ) 한국토양비료학회 1981 한국토양비료학회 학술발표회 Vol.1981 No.26

Electronic Structure of Flavins. Inclusion of Methyl Groups in Molecular Orbital Treatments of Flavins

Pill-Soon Song Korean Chemical Society 1972 대한화학회지 Vol.16 No.3

Various MO methods with differing degrees of sophistication are shown to yield qualitatively consistent results for methyl isoalloxazins. However, with crude methods such as the HMO and ${\omega}$-technique, the choice of Coulomb and resonance integralsis critical, in contrast with simpler molecular systems. The empirical value of ${\omega}$=0.5 appears to be more reasonable than 1.4. Methyl groups in these flaving are best treated by the group orbital approximation. The pseudo-heteroatom approximation overestimates methyl hyperconfiguration with the Pariser-Parrpole SCR MO method. siglet ${\pi}{\rightarrow}{\pi}^*$ transition energies are calculated by the P-P-P method and agree reasonably with the experimental values. 2- and 4-Thioisoalloxazine analogs are also treated. Reactivity indices of the flavin molecule are presented, includeing superdelocalizability. frontier orbital and radical densities. Various aspects of the applications of these indices of the methyl groups on dipolemoments, inozation potentialsm elctron affinities, and spectra are decribed in detail.