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Effects of exploration and molecular mechanism of CsV on eNOS and vascular endothelial functions
Zuo, Deyu,Jiang, Heng,Yi, Shixiong,Fu, Yang,Xie, Lei,Peng, Qifeng,Liu, Pei,Zhou, Jie,Li, Xunjia Techno-Press 2022 Advances in nano research Vol.12 No.5
This study aimed to investigate the effects and potential mechanisms of Chikusetsusaponin V (CsV) on endothelial nitric oxide synthase (eNOS) and vascular endothelial cell functions. Different concentrations of CsV were added to animal models, bovine aorta endothelial cells (BAECs) and human umbilical vein endothelial cells (HUVECs) cultured in vitro. qPCR, Western blotting (WB), and B ultrasound were performed to explore the effects of CsV on mouse endothelial cell functions, vascular stiffness and cellular eNOS mRNA, protein expression and NO release. Bioinformatics analysis, network pharmacology, molecular docking and protein mass spectrometry analysis were conducted to jointly predict the upstream transcription factors of eNOS. Furthermore, pulldown and ChIP and dual luciferase assays were employed for subsequent verification. At the presence or absence of CsV stimulation, either overexpression or knockdown of purine rich element binding protein A (PURA) was conducted, and PCR assay was employed to detect PURA and eNOS mRNA expressions, Western blot was used to detect PURA and eNOS protein expressions, cell NO release and serum NO levels. Tube formation experiment was conducted to detect the tube forming capability of HUVECs cells. The animal vasodilation function test detected the vasodilation functions. Ultrasonic detection was performed to determine the mouse aortic arch pulse wave velocity to identify aortic stiffness. CsV stimulus on bovine aortic cells revealed that CsV could upregulate eNOS protein levels in vascular endothelial cells in a concentration and time dependent manner. The expression levels of eNOS mRNA and phosphorylation sites Ser1177, Ser633 and Thr495 increased significantly after CsV stimulation. Meanwhile, CsV could also enhance the tube forming capability of HUVECs cells. Following the mice were gavaged using CsV, the eNOS protein level of mouse aortic endothelial cells was upregulated in a concentration- and time-dependent manner, and serum NO release and vasodilation ability were simultaneously elevated whereas arterial stiffness was alleviated. The pulldown, ChIP and dual luciferase assays demonstrated that PURA could bind to the eNOS promoter and facilitate the transcription of eNOS. Under the conditions of presence or absence of CsV stimulation, overexpression or knockdown of PURA indicated that the effect of CsV on vascular endothelial function and eNOS was weakened following PURA gene silence, whereas overexpression of PURA gene could enhance the effect of CsV upregulating eNOS expression. CsV could promote NO release from endothelial cells by upregulating the expression of PURA/eNOS pathway, improve endothelial cell functions, enhance vasodilation capability, and alleviate vessel stiffness. The present study plays a role in offering a theoretical basis for the development and application of CsV in vascular function improvement, and it also provides a more comprehensive understanding of the pharmacodynamics of CsV.
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Location Fingerprint Positioning Technology using Bat Algorithm
Song Lixin,Zhou Chuanbin,Pei Heng 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.9
A location fingerprint positioning technology method is proposed based on bat algorithm to improve the defect of traditional indoor location fingerprint positioning technology. These shortcomings include offline heavy workload, limited accuracy and poor robustness. The fingerprint database can generate using the middle point interpolation method and the method that channel attenuation model which instead of offline training stage. This stage realize the function of timing automatic databased updates. After that the database combines K nearest neighbor algorithm with bat algorithm in the stage of matching algorithm to realize the positioning function. Compared with the traditional method, location fingerprint positioning technology using bat algorithm reduces the overall positioning of workload and rapidly respond to the effect of the changing environment. Finally, localization performance test is carried out under the given simulation environment. The result indicates that this method improves the average precision than other algorithms about 23.14%, the vast most of the blind node position error range within 1.5 meters, which shows the advantages of positioning accuracy, robustness and adaptation to the changing environment.
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Junjun Liu,Hongqiao Zhu,Yifei Pei,Heng Zhang,Jian Zhou,Zaiping Jing 한국생체재료학회 2022 생체재료학회지 Vol.26 No.2
Background: Stent-graft-induced inflammation is an independent risk factor for adverse aortic remodeling in aortic dissection. In this context, we asked that whether a methylprednisolone-loaded stent-graft could reduce inflammation and degradation. Methods: First, a coaxial electrospinning technique was used to create a core-shell film with methylprednisolone encapsulated in the inner of poly (L-lactide-co-caprolactone) nanofibers for controllable drug release. Second, an in vitro study was conducted to evaluate the biocompatibility of the nanofiber meshes. Third, the porcine aortic dissection model was developed to investigate the therapeutic effects of the ethylprednisolone-loaded stent-graft. Results: The results demonstrated that the nanofiber-coated film with a methylprednisolone-poly-caprolactone core layer and a poly (L-lactide-co-caprolactone) shell layer could effectively sustain drug release in vitro. In vivo study showed that the methylprednisolone-loaded stent-graft could reduce degradtion of aortic dissection by regulating inflammation. Conclusions: Overall, the controllable drug release by coaxial nanofiber is a promising approach to alleviate aortic inflammation and promote aortic remodeling after stent-graft implantation.
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Koh, Renwei Eric,Sun, Cha Chee,Yap, Yee Ling,Cheang, Pei Ling,You, Ah Heng The Korean Electrochemical Society 2021 Journal of electrochemical science and technology Vol.12 No.2
In this study, Monte Carlo (MC) simulation is conducted with recurrence relation to study the effect of SiO<sub>2</sub> with different particle size and their roles in enhancing the ionic conductivity and lithium transference number of PMMA composite polymer electrolytes (CPEs). The MC simulated ionic conductivity is verified with the measurements from Electrochemical Impedance Spectroscopy (EIS). Then, the lithium transference number of CPEs is calculated using recurrence relation with the MC simulated current density and the reference transference number obtained. Incorporation of micron-size SiO<sub>2</sub> (≤10 ㎛) fillers into the mixture improves the ionic conductivity from 8.60×10<sup>-5</sup> S/cm to 2.35×10<sup>-4</sup> S/cm. The improvement is also observed on the lithium transference number, where it increases from 0.088 to 0.3757. Furthermore, the addition of nano-sized SiO<sub>2</sub> (≤12 nm) fillers further increases the ionic conductivity up towards 3.79×10<sup>-4</sup> S/cm and lithium transference number of 0.4105. The large effective surface area of SiO<sub>2</sub> fillers is responsible for the improvement in ionic conductivity and the transference number in PMMA composite polymer electrolytes.
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Chih-Jen Yang,Yu-Jung Huang,Cheng-Yuan Wang,Chuan-Sheng Wang,Pei-Hui Wang,Jen-Yu Hung,Tung-Heng Wang,Hseng-Kuang Hsu,Hurng-Wern Huang,S.P. Anand Kumar,Ming-Shyan Huang,Ching-Feng Weng 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.1
Toona sinensis is a traditional Chinese herb, and the extracts of T. sinensis leaf possess a variety of biological functions. This study attempted to test the antiproliferative effect of TSL-1 (a bioactive fraction of T. sinensis) in H441 cells (lung adenocarcinoma). The data showed that the antiproliferative effect of TSL-1 on H441 cells is prominent using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. TSL-1-induced apoptosis was confirmed by cell morphology, sub-G1 peak accumulation, cleavage of poly(ADP)-ribose polymerase, and propidium iodide-annexin V double staining. Furthermore, decreased Bcl-2 accompanied by increased Bax (in western blotting) was found with TSL-1 treatment of H441 cells. TSL-1 treatment-induced G1 arrest was concurrent with the down-regulation of protein levels of cyclin D1 and cyclin-dependent kinase 4 in H441 cells. Peroral and intraperitoneal administrations of TSL-1 were performed to evaluate the therapeutic efficacy, and peroral administration of TSL-1 was also used to elucidate the therapeutic efficacy in the H441 cell xenograft model in vivo. The data revealed that TSL-1 treatment inhibited H441 tumor growth in both therapeutic and preventive experiments. Taken together, these results demonstrate that TSL-1 possesses the capability of preventing and alleviating lung cancer proliferation in vitro and in vivo with proven nephrological and hepatic safety and has the potential to be developed as an anti–lung cancer drug.
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