Overexpression of Zoysia ZjCIGR1 gene confers cold stress resistance to zoysiagrass

Yang‑Ji Kim,Dae‑Hwa Yang,Mi‑Young Park,Hyeon‑Jin Sun,Pill‑Soon Song,Hong‑Gyu Kang,Seok‑Cheol Suh,Yong‑Eok Lee,이효연 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1

Zoysia japonica Steud. is a warm-season lawn grass popular in Korea and elsewhere. They are cultivated in many places such as river banks, roadside, and play grounds. However, there still is a disadvantage of frequent mowing, and the grass grows poorly under the chilly conditions. To develop a grass variety that circumvents these drawbacks, we cloned the chitininducible gibberellins-responsive1 gene (CIGR1) from zoysiagrass. The full length of the ZjCIGR1 (Zj; Zoysia japonica Steud.) gene was obtained by 5′/3′ RACE PCR and the phylogenetic tree showed that it belonged to the CIGR1-subgroup in the PAT1-group of GRAS protein family. Expression of the ZjCIGR1 in wild-type plants was confirmed in roots, meristems, leaves, and flowers, especially high in the flowers. The transgenic zoysiagrass was confirmed by PCR using gene-specific primers, phosphinothricin-acetyl-transferase (PAT) strip test, and Southern blot analysis. ZjCIGR1-overexpressing plants acquired tolerance to cold stress displaying morphological phenotypes characteristic of stress resistance. In addition, in the transformants, expression of the ZjCIGR1 as well as cold-regulated (COR) gene was increased compared to the wild-type plants under cold stress condition. These results suggest that ZjCIGR1 gene is an important candidate for regulating cold stress resistance.

私立大學生의 成長發育 및 營養狀態에 關한 硏究 : 中學校 入試有無過程을 通한 比較

朴淳永,具燾書,朴良元,金振浩,南炳執,朴昌植,朴喆斌 慶熙大學校 1980 論文集 Vol.10 No.-

In order to ascertain any possible changes in the physical and the nutritional status of Korean high school students before and after the abolishment of college entrance examination system, an intensive survey was conducted on the physical conditions of the incoming freshmen students (9532 males and 3428 females) of Kyung Hee University from 1972 to 1980. The finding are as below. 1. Physical growth conditions In each of the average physical dimensions of body height, body weight, chest girth and sitting height, a remarkable improvement was recorded for all age groups after the matriculation was abolished. 2. Physical and nutritional indices Relative body weight showed constant values of 35.0 in male and 32.0 in female. Relative chest girth showed the normal chest girth style in all age groups of both sexes. Relative sitting height showed a constant value of 54 for both sexes. The values of vervaeck index of th nutritional status were shown to be between 86-87 in male and 83-94 in female, Pelidisi index 91 and 92-93, Rohrer index of physical status 121-125 and 130-132, and Kaup index 206-211 and 202-210, respectively.

대학수학능력시험 “중국어Ⅰ”의 문항 분류 기준에 관한 재고

金美順(Kim, Mi-soon?Park),朴良淑(Park, Yang-sook) 중국어문학연구회 2013 중국어문학논집 Vol.0 No.80

이면성 및 도플러 심초음파도를 이용하여 진단한 선천성 거대 좌회선지-좌심실 관동맥루

진승원,박준철,이영중,유병현,박현옥,김용주,윤호중,김재형,홍순조,최규보,권종범,원용순,박건 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4

후종골인대 석회화증의 외과적 치료

박수영,박순필,김상진,도종웅,주양자 최신의학사 1988 最新醫學 Vol.31 No.2

트랙타 부착 대형이앙기 개발 및 재배적 실용성

박성태,장익주,정창화,김상열,김재현,문헌팔,양세준,김순철 한국국제농업개발학회 2002 韓國國際農業開發學會誌 Vol.14 No.3

쌀 생산비를 줄이고 대경지 규모화 영농에 적응할 수 있는 농기계 개발을 목적으로 연구개발한 대규모 경지적응 트랙터 부착 대형벼이앙기의 구성과 특징 및 개발시작기의 재배적 실용성 평가결과를 요약정리하면 다음과 같다. 1. 트랙터 부착 12조 대형벼이앙기는 크게 바퀴자국 제거로타리, 문짝형 정지판, 이앙장치부 등으로 구성되어 있다. 2. 트랙터 부착 12조 이앙기는 트랙터 동력을 이용하여 로타리, 정지 및 이앙작업이 동시에 이루어 질 수 있도록 개발하였다. 3. 바퀴자국을 없애는 날은 기존의 로타리 날을 교체하면 되도록 반달형 날로 제작하였다. 4. 이앙기는 이동시는 접을 수 있도록 설계 제작되어 대형화가 가능하므로 기존 6조 승용이앙기에 비하여 이앙작업 능력이 1.5배 이상이었다. 5. 트랙터 부착 대형벼이앙기는 트랙터와 같은 큰 동력원을 이용할 수 있으므로 측조시비기, 긴매트모 탑재 등 다양한 작업기 및 기능을 추가로 부착 가능하도록 설계되었다. 6. 트랙터 부착 대형벼이앙기는 어린모 및 중묘이앙시 결주율은 3.0∼4.3%로 기계이앙기 결주 허용범위 5% 미만이었다. 하지만 무경운 논에서는 이앙작업은 가능하나 부묘율이 다소 높은 점은 보완이 필요하다. To develop a labor saving rice cultivation technology, a new tractor attached rice transplanter has been developed for large scale farming and its field performance was evaluated. The rice transplanter consists of three major parts such as rotary for removing tractor tire furrow, door-shaped soil leveling plate and transplanting equipment. The rice transplanter is operated using the power of the tractor and performs rotary, harrow and seedling transplanting simultaneously. A half moon shaped rotary blade was found to be very efficient to remove tire furrow developed during seedling transplanting and the original rotary blade can be replaced easily with existed rotary blade. The seedling transplanting part of the transplanter was designed to fold into small size and the transplanting efficiency of the transplanter was found to be 1.5 times higher than that of the conventional riding type six row transplanter. Since the transplanter is operated through a strong power of the tractor, the additional attachment such as fertilizer application apparatus and long mat rice seedling carrier is possible. Based on the field performance of the transplanter, although the missing hill percentage showed roughly with 3.0∼4.3% for 10-day and 30-day old seedlings but the percentage is less than 5% which is maximum missing hill permit in the transplanting rice. Seedling transplanting in no-tillage field condition is acceptable but floating seedling percentage is still high and thus further technology improvement for minimizing floating seedling percontage is needed.

중학교 가정 교과 수행평가 방법 및 도구 개발

박선영,조필교,이양심,윤인경,임양순,최창숙,장명희 한국 가정과 교육 학회 1998 한국가정과교육학회지 Vol.10 No.2

The purpose of the study is to develop a Performance Assessment Method & Tools for Home Economics at middle school. Through the content analyses of middle school Home Economics curriculum and 8 textbooks of lst, 2nd, and 3rd grade respectively, teching elements for each content area were drawn and each specific learning objectives were idntified accordingly. In addition, assessment element for student's performance in each teaching element were specified for analysis. Analytic framework for the development of Performance Assessment Method and Tools were proposed by each teaching element. Beased on the analytic framework proposed, a model method and tools were developed by 10 different test measures of description written test, statement written test, oral test, pro and con discussion, performance test, experiment, interview, observation, self-test report, and portfolio. The model proposed in the study has been modified and improved through the feasibility studies of experts' examination and field research application. The model assessment method and tools for the study has been compleeted finally by proposing 45 performance assessment tools for the content areas of food, clothing, housing, resource management and consumerism, human development and family relationship.

지방정부 재가노인복지사업의 적실성 제고방안 : 대구광역시의 일부 사례를 중심으로

김순양, 박병일, 고수정 영남대학교 사회과학연구소 2001 社會科學硏究 Vol.20 No.2

본 논문은 지방정부 단위의 재가노인복지사업의 현황과 문제점을 대구광역시의 사례를 중심으로 실증적으로 분석하고, 이를 통해서 아직 초기단계에 불과한 지방정부에서의 재가 노인복지사업의 적실성 제고방안을 제시하려는 것이다. 이를 위해서 이론적 논의로서 재가 노인복지의 의의, 필요성, 그리고 한국에서의 재가노인복지의 전개과정을 살펴보았다. 그리고 이러한 이론적 논의를 토대로 실증적 연구를 수행하기 위한 분석 틀을 Gilbert와 Terrell의 네 가지 분석차원을 중심으로 구성하였다. 다음에는 이러한 분석 틀에 입각하여 대구광역시의 재가노인복지사업의 현황과 문제점들을 적용범위, 전달체계, 급여내용, 재정의 네 가지 분석차원을 중심으로 고찰하였으며, 이를 토대로 재가노인복지사업의 적실성물 제고할 수 있는 구체적인 방안들을 제시하였다. 그리고 여기서 재가노인복지사업의 범위에는 가정봉사원 파견사업, 주간보호사업, 단기보호사업의 세 가지를 포함하였다.

벼 기계이앙 1회용 종이 육묘상자 개발

박성태,황정동,김상열,문헌팔,양세준,김순철,유남진 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.3

벼 기계이앙 육묘에 주로 사용하고 있는 플라스틱 육묘상자의 문제점을 개선하고 친환경 농업 실현에도 기여할 수 있는 벼 기계이양 종이 육묘상자개발 및 육묘시험 결과를 요약하면 다음과 같다. 1. 폐지 등 혼합물을 고열압착한 종이 육묘상자는 내부 길이가 가로 58㎝, 세로 28㎝, 높이는 2㎝이고 무게는 250g이다. 2. 종이 육묘상자 모의 파종 후 10일~30일 묘소질은 플라스틱 육묘상자 모에 비하여 초장이 1.1~1.8㎝ 짧고 본당 건물중은 0.3~3.7㎎이 떨어졌으나 엽수와 모 충실도는 비슷하였다. 3. 종이 육묘상자 모의 파종 후 30일(중묘) 이앙 시 결주율은 3.3%로 플라스틱 육묘상자 모 결주율 3.2%와 거의 같았고, 출수기, 벼 생육특성 및 수량도 플라스틱 육묘상자와 비슷하였다. 4. 종이 육묘상자의 이점은 플라스틱 육묘상자에 비하여 육묘상자 비용이 약 20% 절감되고 파종 후 운반, 치상 등 작업이 용이하며, 이앙 후 회수, 세척 및 보관관리 노력이 생략된다. 그리고 이앙 후는 논에서 유기물화되므로 친환경농업에도 부합된다. A light and bio-degradable paper tray for seedling raising in machine transplanting rice was developed for the first time and a possibility for rice seedling growth was evaluated. The paper tray was made of mixture of recycling paper and sawdust, and molded under high temperature and pressure. The tray was 58㎝ length x28㎝ width x2㎝ height in size and 250g in weight per tray. The seedling height and dry weight per plant grown at the paper tray were generally 1.1 to 1.8㎝ shorter and 0.3 to 3.7㎎ lighter than those grown at the conventional plastic tray, respectively. However, other characters such as leaf number, seedling health score, missing hill, heading date, panicle and spiketlet number, and milled rice yield were similar to those in the plastic tray. Based on the result of research, the paper tray had following advantages in comparison of the plastic tray. The paper tray could be reduced seedling raising cost by 20% as compared with the plastic tray. Since the paper tray was of lightness even after seedbed soil fill-up, it was more handy and convenient to carry over. Labor input for collecting, washing, and storing the empty tray after seedling transplanting was not needed as the plastic tray. The paper tray was also bio-degraded and transformed into an organic matter, once it was incorporated into soil, which was considered as an environmentally- safe cultivation technology.

교통사고 발생 추이에 관한 연구

양혁준,최봉근,윤태영,최중명,박순영,유동준 慶熙大學校 醫科大學 1997 慶熙 醫大 論文集 Vol.22 No.1