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Park, Kyung Eui,Kim, Sung Ah,Kang, Moon Joo,Kim, Hee Sun,Cho, Sung Im,Yoo, Kyoung Won,Kim, So Yeon,Lee, Hye Jun,Oh, Sun Kyung,Seong, Moon-Woo,Ku, Seung-Yup,Jun, Jong Kwan,Park, Sung Sup,Choi, Young Mi The Korean Society for Reproductive Medicine 2013 Clinical and Experimental Reproductive Medicine Vol.40 No.1
Hypochondroplasia (HCH) is an autosomal dominant inherited skeletal dysplasia, usually caused by a heterozygous mutation in the fibroblast growth factor receptor 3 gene (FGFR3). A 27-year-old HCH woman with a history of two consecutive abortions of HCH-affected fetuses visited our clinic for preimplantation genetic diagnosis (PGD). We confirmed the mutation in the proband (FGFR3:c.1620C>A, p.N540K), and established a nested allele-specific PCR and sequence analysis for PGD using single lymphocyte cells. We performed this molecular genetic analysis to detect the presence of mutation among 20 blastomeres from 18 different embryos, and selected 9 embryos with the wild-type sequence (FGFR3:c.1620C). A successful pregnancy was achieved through a frozen-thawed cycle and resulted in the full-term birth of a normal neonate. To the best of our knowledge, this is the first report of a successful pregnancy and birth using single-cell allele-specific PCR and sequencing for PGD in an HCH patient.
( Sun Ha Choi ),( Ji Hyun Kim ),( Jin Eun Choi ),( Yun Yong Seon ),( Kwon Hee Won ),( Sun Ji Park ),( Ji Eun Park ),( Yong Hoon Lee ),( Hyewon Seo ),( Seung Soo Yoo ),( Jaehee Lee ),( Shin Yup Lee ),( 대한결핵 및 호흡기학회 2019 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.127 No.-
Achaete-scute homolog 1 (ASCL1) is a basic helix- loop-helix transcription factor and is essential in the differentiation of neuroendocrine cells and neural tissues. ASCL1 is frequently overexpressed in small cell lung cancer (SCLC) and plays a crucial role in the pathogenesis of SCLC. This study was conducted to identify the association between single nucleotide polymorphisms (SNPs) in ASCL1 target genes and clinical outcomes of patients with SCLC after chemotherapy. The two-hundred sixty-one patients diagnosed as SCLC were enrolled in this study. The association between 103 SNPs in 58 ASCL1 target genes and the response to chemotherapy and survival of patients with SCLC were analyzed. Among the 103 SNPs, 10 SNPs were significantly associated with the response to chemotherapy, and 19 SNPs were associated with OS in multivariate analyses. Among these, Dopa Decarboxylase (DDC) rs12666409A>T was significantly associated with both worse response to chemotherapy and worse OS (adjusted odds ratio [aOR] = 0.40, 95% CI = 0.18-0.90, P = 0.03; adjusted hazard ratio [aHR] = 1.52, 95% CI = 1.10-2.10, P = 0.01, respectively, under a dominant model). In a stage-stratified analysis, the association was significant only in the extensive stage subgroup (aOR = 0.19, 95% CI = 0.06-0.60, P = 0.01; aHR = 1.73, 95% CI = 1.16-2.56, P = 0.01, respectively, under a dominant model), but not in the limited stage subgroup. These results suggest that DDC rs12666409A>T may be useful markers for predicting the clinical outcomes of patients with SCLC undergoing chemotherapy.
Clinical characteristics and outcomes of lung adenocarcinoma with solid or micropapillary subtypes
( Sun Ha Choi ),( Jae Yong Park ),( Yong Seon Yoon ),( Hee Won Kwon ),( Ji Eun Park ),( Sun Ji Park ),( Yong Hoon Lee ),( Hyewon Seo ),( Seung Soo Yoo ),( Shin Yup Lee ),( Jaehee Lee ),( Seung Ick Cha 대한결핵 및 호흡기학회 2019 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.127 No.-
Objective: The aim of this study was to investigate the clinical characteristics and outcomes of lung adenocarcinoma with solid or micropapillary (S/MP) subtypes in surgically treated patients. Methods: We retrospectively reviewed medical records and pathologic reports of 410 patients with lung adenocarcinoma who underwent curative resection. Clinical features and recurrence free survival were compared between groups with S/MP subtypes (S/MP+, n=203) and without S/MP subtypes (S/MP-, n=207). Result: S/MP subtypes were present in 203 patients (49.5%), and 141 (34.4%) had ≥ 5% of S/MP proportion. S/MP subtypes were more frequently observed in patients with male gender (57.6% vs. 42.0%, P=0.002), tumor > 3cm (62.3% vs. 37.4%, P<0.001), and positive lymph node stasis (85.9 vs. 40.9%, P<0.001) compared to female gender, tumor ≤ 3cm, and negative lymph node stasis, respectively. Recurrence and death were more frequent in S/MP+ group compared with S/MP- group (24.1% vs. 1.9%, P<0.001 and 5.4% vs. 1.4%, P=0.02). The tumor with S/MP proportion of < 5% (S/MP< 5%) were more frequent in patients with tumor > 3 cm (34.8% vs. 14.3%, P<0.001) and positive lymph node metastasis (60.9% vs. 18.7%, P<0.001) compared to tumor ≤ 3cm and negative lymph node metastasis, respectively, and more frequently associated with recurrence and death (22.6% vs. 1.9%, P<0.001 and 6.5% vs. 1.4%, P=0.05) compared with S/MP- group. Survival analysis indicated that S/ MP+ and S/MP<5% were associated with shorter recurrence free survival compare with S/MP- (HR=9.1, 95% CI=3.1-26.8, P<0.001; and HR=10.1, 95% CI=2.9-35.5, P<0.001, respectively). S/MP+ and S/MP<5% were more powerful predictor of recurrence than T or N stage in multivariate analyses.
Shim, Sun-Yup,Kang, Hye-Sook,Sun, Hyeon-Jin,Lee, Young-Ju,Park, Jeong-Ro,Chun, Soon-Sil,Song, Young-Hwan,Byun, Dae-Seok 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.2
In an ongoing search of compounds from natural sources that exhibit anti-allergic properties, 3 flavonoids were isolated from the ethyl acetatefraction of methanol extracts of gujeolcho (Chrysanthemum zawadskii var. latilobum), with guided histamine release inhibitory effects. The isolated compounds were identified as eriodictyol-7-O-${\beta}$-D-glucuronide, 5,7-dihydroxy-4-chromene, and 6-methoxyluteolin based on spectroscopic evidences including NMR and MS spectra. These results suggest that gujeolcho extracts and 3 compounds would be candidates as therapeutic or preventive agents for allergic reaction.
( Sunji Park ),( Jaehee Lee ),( Ji Eun Park ),( Sun Ha Choi ),( Hyewon Seo ),( Seung Soo Yoo ),( Shin Yup Lee ),( Yu Kyung Kim ),( Seung Ick Cha ),( Jae Yong Park ),( Tae In Park ),( Chang Ho Kim ) 대한결핵 및 호흡기학회 2020 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.128 No.-
Background The cause of epithelioid granulomatous inflammation varies widely depending on the affected organ, geographic region, and whether the granulomas morphologically contain necrosis. Compared with other organs, the etiological distribution and morphological patterns of pleural epithelioid granulomas have rarely been investigated. We evaluated the final etiologies and morphological patterns of pleural epithelioid granulomatous inflammation in a country with an intermediate tuberculosis (TB) prevalence. Methods The cases who had granulomatous pleural histology on pleural biopsy, retrospectively were reviewed and their final etiologies were identified. Patient with tuberculous pleurisy (TB-P) were classified into 2 groups according to the presence or absence of microbiological evidence: confirmed and probable TB-P. The clinical, blood, radiological, pleural fluid, and histological data were collected and compared between the confirmed and probable TB-P groups and between the confirmed TB-P and non- TB-P groups. Results Of 83 patients with pleural granulomas, 50 (60.2%) had confirmed TB-P and 29 (34.9%) had probable TB-P. Four patients (4.8%) with non-TB-P were diagnosed. With the exception of microbiological Results, there was no significant difference in patient characteristics and granuloma patterns between the confirmed TB-P and non-TB-P groups, or between patients with confirmed and probable TB-Ps. Additionally, anti-TB treatment outcomes were not significantly different between the confirmed and probable TB-P groups. Conclusions These findings suggest that most pleural granulomatous inflammation (95.2%) was attributable to TB-P in a TB-endemic area and that the granuloma patterns contributed little to the prediction of final diagnosis compared with other organs. However, the clinician should make active effort to find microbiological evidence for differentiation between TB-P and non-TB-P.
Butyrate-treated M2 Macrophage Polarization is CECR1-dependent
( Ji Eun Park ),( Ha Jeong Kim ),( Sunji Park ),( Sun Ha Choi ),( Yong Hoon Lee ),( Hye Won Seo ),( Seung Soo Yoo ),( Shin Yup Lee ),( Jaehee Lee ),( Seung Ick Cha ),( Jae Yong Park ),( Chang Ho Kim ) 대한결핵 및 호흡기학회 2020 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.128 No.-
Background The Cat Eye Syndrome Critical Region, Candidate 1 (CECR1) gene and its product adenosine deaminase 2 (ADA2) are highly expressed by macrophages. Patients with tuberculous pleural effusion (TPE) usually have elevated ADA2 levels in pleural fluid. In terms of immunomodulatory functions of intestinal metabolite butyrate, it may affect CECR1 expression in patients with TPE. We examined the effect of butyrate on CECR1 expression of macrophages and the role of CECR1 on butyrate-related immune response. Additionally, relationship between ADA2 and macrophage phenotype in pleural fluid of patients with TPE was investigated. Methods Expression of CECR1 was evaluated in LPS-stimulated and/or butyrate treated THP-1 cells. The role of CECR1 on butyrate-induced immune response was evaluated using siRNA transfected THP-1 cells. Correlation between the levels of chemokines associated with M1/M2 macrophage phenotype and ADA2 in pleural fluid of patients with TPE were analyzed. Results Butyrate promoted the expression of CECR1 and M2-macrophage marker in THP-1 cells. In LPS-stimulated THP-1 cells, butyrate facilitated M2-macrophage marker/anti-inflammatory cytokine expression and inhibited M1-macrophage marker/pro-inflammatory cytokine expression in a CECR1-dependent manner. Among M1 chemokines measured, there was a significant negative correlation between pleural fluid CCL21 and ADA2 levels and between CCL25 and ADA2 levels in patients with TPE (CCL21; Spearman r = -0.478, p = 0.050: CCL25; Spearman r = -0.808, p<0.001). Among M2 chemokines measured, there was a significant positive correlation between TGF-β and ADA2 levels and between IL-22 and ADA2 levels in pleural fluid of patients with TPE (TGF-β; Spearman r = 0.603, p<0.001: IL-22; Spearman r = 0.470, p = 0.050). Conclusions Butyrate enhanced M2-macrophage marker/anti-inflammatory cytokine expression of LPS-stimulated THP-1 cells in a CECR1- dependent manner. ADA2 may exert anti-inflammatory effect during the process of pleural inflammation in patients with TPE.
Lee Jaehee,Park Sunji,Park Ji Eun,Choi Sun Ha,Seo Hyewon,Yoo Seung Soo,Lee Shin Yup,Kim Yu Kyung,Cha Seung-Ick,Park Jae Yong,Park Tae In,Kim Chang Ho 대한의학회 2021 Journal of Korean medical science Vol.36 No.1
The cause of epithelioid granulomatous inflammation varies widely depending on the affected organ, geographic region, and whether the granulomas morphologically contain necrosis. Compared with other organs, the etiological distribution and morphological patterns of pleural epithelioid granulomas have rarely been investigated. We evaluated the final etiologies and morphological patterns of pleural epithelioid granulomatous inflammation in a tuberculosis (TB)-prevalent country. Of 83 patients with pleural granulomas, 50 (60.2%) had confirmed TB pleurisy (TB-P) and 29 (34.9%) had probable TB- P. Four patients (4.8%) with non-TB-P were diagnosed. With the exception of microbiological results, there was no significant difference in clinical characteristics and granuloma patterns between the confirmed TB-P and non-TB-P groups, or between patients with confirmed and probable TB-Ps. These findings suggest that most pleural granulomatous inflammation (95.2%) was attributable to TB-P in TB-endemic areas and that the granuloma patterns contributed little to the prediction of final diagnosis compared with other organs.