대전광역시 월평공원과 전북 격포해수욕장 주변 야생화와 토양들로부터 야생효모의 분리 및 국내 미기록 효모들의 특성과 생리 활성

장지은 ( Ji-eun Jang ),박선정 ( Seon-jeong Park ),이종수 ( Jong-soo Lee ) 한국균학회 2021 韓國菌學會誌 Vol.49 No.1

많은 사람들이 다녀가는 도시공원과 해변 환경의 효모 특성을 알아보기 위해 대전광역시 월평공원 갑천 누리길과 내원사 주변의 야생화와 토양 75점에서 모두 88균주의 야생 효모들을 분리, 동정하였다. 또한 전북 부안의 격포해수욕장과 격포항 주변 야생화와 토양 35점에서 30균주의 야생 효모들을 분리하였다. 이들 분리 야생 효모들 중A. porosum ASCM32-1, A. scarabaeorum WP49-1, C. athensensis WP4-90-3, C. berkhoutiae WP34-2, C. bracarensis WP35-1, Cy. amylophila GP19-1, K. gamospora WP9-1, M. carpophila BN-17-1, T. namnaoensis WP4-89-1, V. taibaiensis WP13-2 등 10종의 국내 미기록종 후보 균주들을 선별하여 균학적 특성을 확인하였다. 이들 미기록 효모들은 주로 구형~타원형으로 모두 출아방식으로 영양 증식을 하였고V. taibaiensis WP13-2와 K. gamospora WP9-1, C. athensensis WP4-90-3 외 7종의 미기록 효모들은 자낭포자를 형성하지 않은 무포자 효모들이었으며A. porosum ASCM32-1만이 의균사를 생성하였다. 대부분의 균주들은 YPD 배지와 YM 배지에서 생육하였고V. taibaiensis WP13-2와K. gamospora WP9-1, T. namnaoensis WP4-89-1 균주들은 15% NaCl을 함유한 YPD 배지에서 생육하는 호염성 효모들이었고, Cy. amylophila GP19-1 균주는 40˚C에서도 생육하였다. C. athensensis WP4-90-3 균주 등 5균주들이 10% 에탄올 내성을 보였고C. athensensis WP4-90-3 균주는 Li²⁺ 등 많은 중금속들에 대해서 내성을 보였다. 국내 미기록 효모들과 비병원성 야생 효모 균주 무세포추출물들의 β-glucuronidase 저해 활성은 C. oleophila WP5-19-1의 무세포 추출물이 49.0%로 가장 높았고 미기록 효모인K. gamospora WP9-1의 무세포 추출물도 27.7%를 보였다. 호중구 elastase 저해 활성은W. anomalus HO9-2가 38.4%로 우수하여 건강 소재로 생산에 매우 유용한 균주로 활용될 것으로 기대된다. This study aimed to isolate wild yeasts obtained from flowers and soil of the Wolpyung park, Daejeon city and Gykpo beach, Buan, Jeollabuk-do in Korea, and to further characterize previously unrecorded wild yeast strains. In total, 88 strains of 62 different species of wild yeasts were isolated from 75 samples obtained from the Wolpyung park. Among these, six strains of Trichosporon moniliiforme and four strains each of Papiliotrema flavescens and Candida melibiosica were isolated. Additionally, 39 strains of 30 different species of wild yeasts were isolated from 35 samples collected from the Gykpo beach. Among the 127 isolated wild yeast strains, 10 strains, including Apiotrichum porosum ASCM32-1, were previously unrecorded. All the 10 previously unrecorded yeasts were oval or global in shape, and three strains, including Candida athensensis WP4-90-3, formed spores. Three strains, including Vishniacozyma taibaiensis WP13-2, were halophilic yeasts which grew in 15% NaCl-containing YPD(yeast extract-peptone-dextrose) medium. Five strains, including C. athensensis WP4-90-3, showed 15% ethanol resistance. Cell-free extracts from Candida oleophila WP5-19-1 and Wickerhamomyces anomalus HO9-2 showed the highest β-glucuronidase inhibitory activity (49.0%) and neutrophil elastase inhibitory activity (38.4%), respectively.

제3분과 : 야생동물 ; 국립공원 산불통제구간내 야생동물 서식현황 분석 -지리산국립공원 노고단~피아골계곡을 중심으로-

강병선 ( Byung Seon Kahng ),박기연 ( Ki Yeon Park ),김은창 ( Eun Chang Kim ),이재윤 ( Jae Yoon Lee ),지기선 ( Ki Seon Ji ),최형진 ( Hyung Jin Choi ),오현경 ( Hyun Kyung Oh ) 한국환경생태학회 2013 한국환경생태학회 학술대회지 Vol.2013 No.1

우리나라 전 국토의 6.58%를 차지하고 있는 국립공원은 생태계 현황을 대표하는 지역으로 한국에 서식하는 생물종 39,150종 중 40%에 해당하는 15,727종이 서식하고 있다(국립공원관리공단, 2012). 특히 환경부에서 지정한 멸종위기종 221종 중에서 65%에 해당하는 144종이 서식하고 있어 인간의 간섭 및 개발압력으로부터 마지막 피난처의 중요한 역할 또한 수행하고 있다. 국립공원은 자연생태계와 자연 및 문화경관 등을 보전하고 지속가능한 이용을 도모함을 목적으로 하는 자연공원법에 의하여 지정되는 자연공원의 하나로서 자연생태계의 보전뿐만 아니라 지속가능한 이용 또한 고려되어야 한다. 즉, 생물종 서식처의 보존 뿐만 아니라 탐방객의 이용 또한 고려되어야 하며 이를 위해서는 생물종의 서식현황 분석은 반드시 선행되어야 할 과제이다. 그러나 생물종이 다양하고 서식처가 광범위하여 종의 전체적인 서식현황을 분석에는 많은 시간과 비용이 수반된다. 따라서 시간과 공간이 제한되는 산불조심기간 중 산불통제 구간을 대상으로 야생동물의 서식현황을 분석해 보고자 하였다. 아울러 구례군 지역에 서식하는 야생동물의 서식현황을 분석하여 보호지역 관리에 기초자료를 제공하고자 하였다. 월 1회 정기적으로 탐방로를 중심으로 야생동물의 식흔, 배설물 등 흔적을 2010년부터 2012년까지 최근 3년간 모니터링 하였으며, 모니터링 시 GPS 좌표 측점, 사진촬영, 주변환경조사 등을 병행하였다. 아울러 무인센서카메라를 10대 설치하여 실체를 확인하였으며, 조사된 자료는 GIS DB로 최근 3년간, 지리산국립공원 노고단~피아골계곡에서 조사된 야생동물 흔적은 124건으로 삵 59건, 담비 27건, 족제비 21건, 멧토끼 10건, 멧돼지 4건, 고라니 2건, 오소리 1건 등이었다. 이중 삵과 담비는 멸종위기종으로서 86건으로 발견종의 69%를 차지하고 있었다. 특히 봄과 가을철 산불통 제기간 중에는 월평균 19회 관찰되었는데 이는 다른 기간 평균 4회 보다 5배정도 많은 횟수로 탐방객의 출입이 제한되었을 때 위협요인이 감소됨으로서 서식여건이 보다 안정화되어 훨씬 많이 발견되고 있음을 알 수 있었다. 2010년부터 2012년 지리산국립공원 구례지역에서 조사된 야생동물 698건의 서식 분포특성을 분석한 결과 삵 286건, 담비 139건, 족제비 95건, 수달 53건, 오소리 30건, 멧돼지 30건, 멧토끼 29건, 너구리 16건, 고라니 13건, 노루 4건, 하늘다람쥐 3건 등이었다. 특히 멸종위기종인 삵, 담비, 수달, 하늘다람쥐 등이 481건으로 68%를 차지하고 있어 멸종위기 야생동물의 출현빈도가 매우 높음을 알 수 있었다. 최근 3년간 야생동물 서식현황을 분석한 결과 타기간 보다 산불방지기간에 출현빈도가 높아 탐방객의 출입에 따른 위협요인을 계량적으로 파악할 수 있었으며, 특히, 봄철 산불방지기간은 야생동물의 번식기라는 점에서 보다 안정된 서식처를 제공한다는 의의가 있을 수 있다. 또한 멸종위기종의 흔적이 전체종의 68%가 발견됨으로서 멸종위기종의 서식처를 제공하고 있음을 알 수 있었다. 향후 이러한 결과를 바탕으로 각 야생동물에 대한 서식처 분석과 더불어 이를 통한 관리방안이 수립될 필요성이 있다.

갑상선 유두암이 병발된 가족성 대장 용종증 1예

백종현,소창배,이은영,이지숙,신은경,박은호,서정아,양재홍,송준영,박선자,박요한,최영식 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Familial adenomatous polyposis (FAP) is an autosomal dominant syndrome, typically characterized by multiple colorectal adenomas and increased incidence of colorectal carcinomas if it is not treated. Moreover, a variety of extracolonic manifestation are seen. The prevalence of thyroid tumors developing in patients with FAP is about 1∼2%. Generally, papillary thyroid cancer indicates low mortality but ,however, as people get older, the cancer becomes more aggressive. So, the operation is required. Recently we experienced a case of FAP, presenting with papillary thyroid carcinoma, and reported with a brief review of literatures.

Multi‑tissue lipotoxicity caused by high‑fat diet feeding is attenuated by the supplementation of Korean red ginseng in mice

Seon‑A Jang,Seung Namkoong,이성률,Jin Woo Lee,Yuna Park,Gyeongseop So,Sung Hyeok Kim,Mi‑Ja Kim,Ki‑Hyo Jang,Alberto P. Avolio,Sumudu V. S. Gangoda,Hyun Jung Koo,Myung Kyum Kim,Se Chan Kang,Eun‑Hwa Sohn 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.1

Background Excessive intake of fat, one of the causes of obesity, is associated with low-grade infammation in various susceptible organs and eventually causes tissue toxicity. This study examines the multifaceted suppressive efects of Korean red ginseng extract (KRG) on high-fat diet (HFD)-induced lipotoxicity and infammatory responses in the aorta, liver, and brain. Methods Male C57BL/6 mice were fed HFD with or without KRG for 12 weeks. The improvement efect in KRG on lipotoxicity and infammatory potential was determined in the blood and the aorta, liver, and brain tissues. Results KRG signifcantly inhibited 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activity by >20% in vitro. KRG supplementation suppressed HFD-associated body weight gain, lipid profle changes, and excessive fat deposition in the liver and increased leptin, insulin, and ALT levels in the blood. Infammatory markers in the aorta, liver, and brain were also signifcantly reduced by KRG treatment. In microvascular endothelial cells, the 15% cyclic stretch-mediated upregulation of ICAM-1 and vascular cell adhesion protein-1 (VCAM-1) expression was signifcantly attenuated in the presence of KRG. Conclusion KRG supplementation attenuates HFD-mediated body weight gain, lipid profle changes, and multi-tissue infammatory responses.

저칼슘식이 생쥐 하악골에서 파골세포의 Tartrate저항성 산성인산분해효소 활성에 대한 세포화학적 연구

박은주,임도선,김현만,고재승 대한구강해부학회 1994 대한구강해부학회지 Vol.18 No.1

The present study was carried out to exanine the morphological changes and TRACP (Tartrate resistant acid phosphatase) activity in the resorption of mandibular bones of mouses resulted, established by low calcium diet. Mice were divided into two groups; one was the calcein group for fluorescent microscopy, the other was the non-calcein group for light and electron microscopy. After calcein injection, mice(ICR) were fed either a low calcium diet (0.01% Ca, 0.75% P) or control diet (0.1% Ca. 0.75% P). Non-calcein group were also fed a low calcium diet or control diet. Mice were sacrificed on 3, 7 and 14 days. For fluorescent microscopy study, fixed undecalcified alveolar bones were embedded in Epon 812, and ground sectioned to study the new bone formation after calcein injection. For the TRACP histochemical study, fixed decalcified alveolar bones were incubated in p-NPP (p-nitrophenyl phosphate) media. For the transmission electron microscopic study, incubated alveolar bone were post fixed in OsO_(4), embedded with Epon 812 for the study of TRACP localzation and activity. The observed results were as follows. 1. Low calcium diet induced bone resorption in buccal and lingual sides of alveolar bone, while the lingual side of alveolar bone was not resorbed in the control group. Bone resorption increased over the period of low calcium diet. 2. The alveolar bone proper of the lingual alveolar bone continued to deposit new bone of the periodontal side even after the low calcium diet. 3. After low calcium diet TRACP activity increased in the cytoplasm of osteoclast, extracellular ruffled border. TRACP activity was also found at the eroded bone matrix after bone resorption, and in the cytoplasm of detached osteoclast. In the immature osteoclast enzyme activity was confined in rER. No enzyme activity was found in the osteoblast.

건강한 성인에서 발병한 칸디다와 연관된 위궤양 1례

박지훈,이상혁,김희,이재호,박성재,지삼룡,양성연,박은택,이연재,설상영,정정명,강미선 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Fungus such as Candida albicans is a normal flora that is frequently found in the oral cavity, gastrointestinal tract, genitourinary tract, vaginal mucosa in a normal person. However, candida can cause opportunistic infection in an immune compromised host. Candidiasis has broad spectrum of disease from mucocutaneous infection to invasive or disseminated infection. But, it is rarely reported that candida is associaed with gastrointestinal tract disease in a healthy adult. The case of gastric ulcer associated with candida particularly in a health adult is reported with relevant literature.

Dissolved Air Flotation(DAF) 접촉조의 수리동력학적 조건이 입자의 제거에 미치는 영향

유제선,김성훈,신은허,박희경 대한상하수도학회 2003 상하수도학회지 Vol.17 No.1

Hydrodynamic conditions for optimum solids capture within the contact zone, which are governed by the geometry and flow, are not yet well understood. In this research, dimensionless parameters which represent hydrodynamic conditions in the contact zone were derived, using the Buckingham π theorem. Those parameters are Reynolds No. (Re), Froude No. (Fr), Weber No. (We), Local Weber No., and Bubble Capacity No. (Bc). The particle removal rate was investigated by changing each value of the dimensionless parameters. The experimental results indicate that particle removal rate, when gravity dominates and the flow is semi-turbulent (Fr < 0.3, Re < 8000), is higher than when inertia dominates and flow is turbulent. For the We, higher removal rates were obtained in the range of the We < 40. Relative to the Local We, smaller bubbles which are influenced more by surface tension than inertia, demonstrated a higher particle removal rate than larger bubbles. For the Bc, the optimal range is from 0.3 to 0.5. We suggest that these dimensionless parameters can be used to diagnose the performance of and to design a DAF reactor.

乾癬患者 臨床報告

吳恩英,朴惠宣,池鮮渶,具德謨 동의대학교 한의학연구소 2001 동의ㆍ경산 한의학 학술대회 Vol.5 No.-

A Reports were done on proriasis which were treated by medications of Sasang Constitution and Constitution-Acupunture in Dept. surgery, opthalmology &tolaryngology, college of Oriental Medicine, Kyungsan University, Pohang, Korea, from May 30. 2000 to JUN 30 2001. we gave score and checked involvement, plaque, erythematous popules, invasiveness according to psoriasis area and severity index. The results were as follows: 1. Sasang Constitution classification was Soyanggin 14(93.33%), Taeumgin 1(6.67%) in the 15 cases. Hyungbangsabaeksan is used in 9 cases(60%), Yanggeuksanhoatang is used in 3 cases(13.11%), Geopungchunggisan is used in 3 cases(20%), Hyungbangdojucksan is used in 1 case(6.57%). 2. In the 9 cases, we checked for over 4 weeks, clinical severity of involvement, plaque, total score(psoriasis area and severity index) was siginificantly decreased(p<05). clinical severity of erythematous popules, itching was decreased but lt is not siginificant(p>05). 3. The mean duration of treatmemt was 73.3 days, result of Excellent or Good improvement was showed on over 120 days. These resilts indicate that Sasang Constitution and Constitution-Acupuntuer treatment is effetive on psoriasis and the more study is needed.

MHC class Ⅰ분자들에 의해 제시되는 Epitope을 인지하는 CD8+ T림프구의 결핵균 감염에 대한 면역반응의 연구 : 결핵환자와 PPD+ 건강개체에 존재하는 결핵균 항원에 특정한 CD8+ T세포 Evidence of M. tuberculosis Specific CD8+ T Cells in TB Patients and PPD+ Healthy Individuals

조장은,이경화,박승규,천선희,조상래,조성애 Official Journal of the Korean society for immunol 2003 Immune Network Vol.3 No.3

우측 대장에 발생한 연소성 용종 1예

이준식,박성한,홍현진,안광순,김지연,배용목,이은영,박선자 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Juvenile polyp, also known as a retention polyp because they contain mucin cysts, is a relatively common hamartomatous lesion in chlidhood. It occurs in 1-2% of children and young adults; however it is uncommon in adulthood. Juvenile polyp is rare in the first year of life and is thus presumed to be acquired and not congenital. It is pedunculated, 2-3 cm in size, red-tan in colour, smooth and always shows fluid filled cystic spaces on cut surface. Histologically, Juvenile polyps are hamartomatous with distended, mucus-filled glands, often with cystic dilatation and edematous lamina propria containing abundant vasculature. The most common manifestation is rectal bleeding produced by auto-amputation of the polyp. Juvenile polyp has been considered not to be at increased risk of developing carcinoma. However, atypical epithelium is observed in some lesions, especially in larger polyps, which may give rise to adenoma and subsequent malignant transformation. Therefore the diagnosis of solitary juvenile polyp should not be made only with colonoscopy and biopsy, but should be totally resected endoscopically for pathologic evaluation. About 90% of the lesions are solitary and localized within 20cm from anal verge. In Korea, there are some reports of juvenile polyps located in the rectosigmoid colon. We report one case of juvenile polyp in adolescent that presented as a hematochezia & abdominal pain, which is located at right colon.