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Comparison of Glucosinolates Contents in the Germplasm of Different Cruciferous Vegetables
On-Sook Hur,Jung-Bong Kim,Jung-Sook Sung,Sang Gyu Kim,Jae-Gyun Gwak,Sukyeung Lee,Yu-mi Choi,Do yoon Hyun,Myung-chul Lee,Hyung-Jin Baek,Binod Prasad Luitel,Kyoung-Yul Ryu,Ho-Cheol Ko 한국원예학회 2015 한국원예학회 학술발표요지 Vol.2015 No.10
Hur, On-Sook,Chang, Dong-Chil,Kim, Sun-Lim,Ok, Hyun-Chung,Kim, Jung-Tae,Chun, Chang-Hoo The Plant Resources Society of Korea 2011 한국자원식물학회지 Vol.24 No.6
The composition of free sugar, free amino acid, and fatty acid in tubers of the cultivars 'Superior', 'Sinnamjak', and 'Chubaek' were evaluated at the two sites with different altitude, Gangneung (20 m altitude) as lowland and Daekwallyeong (760 m altitude) as highland. The average reducing sugar content of the three potato cultivars harvested in Daekwallyeong and Gangneung was 1.1% and 2.3%, respectively, which showed two-fold difference between the two locations. Average total sugar content was also two-folds lower in potatoes harvested in Daekwallyeong (6.3%) than those harvested in Gangneung (12.2%). Average content of free amino acids of the three potato cultivars harvested in Daekwallyeong and Gangneung was 1,325 mg/100g and 1,051 mg/100g, respectively. The cultivar 'Chubaek' has the highest amino acid content among the three tested cultivars. Potatoes from Daekwallyeong have higher unsaturated fatty acid levels than those from Gangneung. During the tuber development, unsaturated fatty acids levels increased in tubers grown in Daekwallyeong, but decreased in those grown in Gangneung. These results indicate that the quality of potatoes from Daekwallyeong is from Gangneung for food processing purpose for human consumption.
Screening of Resistance to Gray Leaf Spot in Tomato Germplasm
On-Sook Hur,Bora Geum,Na-Young Ro 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
The resistance of tomato (Solanum lycopersicum) to the gray leaf spot disease caused by Stemphylium species is conferred by a single incompletely dominant gene, Sm. The resistance gene was introgressed into cultivars from the wild species S. pimpinellifolium on chromosome 11. To select resistant genetic resources to gray leaf spot, we have screened 200 tomato accessions conserved at National Agrobiodiversity Center by inoculum with gray leaf spot. Also, we have genotyped with the marker, Sm_D3 positioned in Solyc11g018720. Seventeen accessions including IT173883 were resistant to Stemphyllium sp. developing necrotic local regions at inoculated leaves as primary symptoms. Selected accessions of S. pimpinellifolium will hopefully contribute for use as donor parents for breeding programs with a new resistant source of gray leaf spot in tomato commercial varieties.
Evaluation of 200 Tomato Wild Accessions for Resistance to Tomato Spotted Wilt Virus (TSWV)
On-Sook Hur,Na-Young Ro,Sukyeong Lee,Aejin Hwang,Seonghoon Kim,Bich-Saem Kim,Awraris Derbie Assefa,Bum-Soo Hahn 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
Tomato spotted wilt virus (TSWV) is an economically damaging pathogen of tomatoes. Typical symptoms of TSWV include wilting or stunning plants and chlorotic or necrotic rings on leaves and fruits. The effective management for TSWV is breeding tomato cultivars containing the Sw-5 from an unknown Solanum peruvianum. Unfortunately, several Sw-5 resistance-breaking strains of TSWV have been identified. Sw-7 from S. chilense accession LA1938 is an alternative locus conferring resistance to TSWV. The objective of this study was to find tolerant or resistant germplasm to TSWV. A total of 200 tomato wild accessions were evaluated for the resistance to TSWV using a bioassay with strain TSWV-SW-TO2. IT173785 (S. peruvianum) and 5 accessions of S. pimpinellifolium including TI173719 were showed apparent recovery or no symptom on upper leaves at 4 weeks later after inoculation. We will conduct the high resolution DNA melting analysis (HRM) with Sw-5 and Sw-7. These selected accessions will hopefully contribute for new source of resistance in tomato for protection against TSWV.
On-Sook Hur,Sang Gyu Kim,Ho-Cheol Ko,Su Ran Ahn,Jung-Sook Sung,Na-Young Ro,Sukyeung Lee,Yu-mi Choi,Do yoon Hyun,Kyoung-Yul Ryu,Hyung-Jin Baek 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
This study aimed to evaluate 105 tomato accessions conserved in National Agrobiodiversity Center regarding their resistance to Ralstonia solanacearum, a soil-borne vascular bacterium that causes lethal wilt diseases of a wide range of crops worldwide. All the accessions are Solanum lycopericum var. lycopersicum including cultivar or breeding lines. At the four leaf stage, the seedlings were inoculated by drenching the soil with the bacterial suspension concentrated of 108 CFU/ml. Plant roots were wounded before inoculation by cutting with the knife. Seven accessions including IT 32899 were rated as resistant, while other 98 accessions were rated as susceptible. IT 32899 scored 0.1 of disease rate and 0.7 of disease index. The selected accessions will be used as a material to reveal the mechanism of wilt tolerance and to identify the host gene involved in defense response.
수집지가 다른 홍화자원의 형태적 및 생화학적 특성 변이
Jung Sook Sung,Ho Cheol Ko,On Sook Hur,Sang Gyu Kim,Jung Ro Lee,Binod P. Luitel,Yong Hwa Lee,Young Seok Jang,Jae Gyun Gwag,Hyung Jin Baek,Kyoung Yul Ryu 한국국제농업개발학회 2016 韓國國際農業開發學會誌 Vol.28 No.1
One hundred seventy two accessions of safflower, collected in four countries were investigated for their morphological and biochemical characters in 2014. The accessions were categorized into two groups; South-Central (S-C) Asia and South-West (S-W) Asia, and each group was represented the accessions of two countries. Variation in morphological and biochemical characters was observed between two groups of accessions. The average value of seed weight and range of variability were higher in S-C Asia accessions while S-W accessions exhibited the variation in plant height, leaf length and days to flowering. The average value of oleic and total oil content were higher in S-C Asia accessions, and the values were 19.8%, and 231.4 mg.g-1, respectively, while the range of variability for total oil content was higher in S-W accessions. Plant height exhibited a significant positive correlation with days to flowering (r = 0.625**). Palmitic acid had positively correlated with stearic acid (r = 0.282**) and linoleic acid (r = 0.444**). Oleic and linoleic acid showed a strong negative correlation (r = -0.977**). The first three principal components explained 57% of the total variation. Morphological and biochemical variation exist in different groups of accessions could be useful to breeder for developing new safflower cultivars with high oil quantity and quality.
Jung Sook Sung,Ho Cheol Ko,On Sook Hur,Sang Gyu Kim,Jung Ro Lee,Binod P. Luitel,Jae Gyun Gwag,Hyung Jin Baek,Kyoung Yul Ryu 한국국제농업개발학회 2015 韓國國際農業開發學會誌 Vol.27 No.3
사우디아라비아 북부지역에서 수집된 홍화 총 100자원에 대 한 총폴리페놀 함량 및 항산화 활성을 평가하여 항산화활성이 높은 홍화자원을 선발하고자 연구를 수행하였으며 그 결과는 다음과 같다. 1. 총 100자원의 홍화유전자원에 대한 총폴리페놀 함량은 14.2 ± 0.41 μg GAE mg−1dw에서 81.6 ± 1.56μg GAEmg−1dw 까지 분포하였다. DPPH 라디컬 소거능은 1.6 ± 0.07 μg ASC mg−1 dw에서 14.1 ± 0.23 μg ASCmg−1 dw까지 활성을 나 타냈다. ABTS 활성은 34.5 ± 0.70 μg Trolox mg−1 dw에서 156.5 ± 3.06 μg Trolox mg−1 dw까지 평가되었다. 항산화활성 을 측정한 DPPH와 ABTS 활성간에는 상당한 유의성(r = 0.954**)을 보였다. 2. 홍화자원 100자원의 총폴리페놀 함량 및 항산화활성 결 과값에 대한 상호관계를 분석하여 활성이 높은 그룹(Group1)을 얻었다. Group1에 포함된 8개의 홍화자원은 나머지 그룹에 속한 자원들에 비해 항산화활성이 높았으며, 이중 K185841, K185879, K185863은 Group1내의 다른 자원들보다도 항산화 활성이 상당히 높아 홍화육종이나 기능성물질연구에 좋은 소 재가 될 것으로 사료된다. This study was undertaken to select genetic resources of safflower with high antioxidant activities. A total of 100 accessions were obtained from the National Agrobiodiversity Center (NAC) of Korea. Total polyphenol contents (TPC) and antioxidant activities of 1,1-diphenyl-2-picryl-hydrazil (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) in 75 % ethanol seed extract of safflower germplasm were determined. TPC ranged from 14.2 ± 0.41 to 81.6 ± 1.56 μg GAE mg-1dry weight (dw). Safflower seed extracts showed variation in DPPH antioxidant activities ranging from 1.6 ± 0.07 to 14.1 ± 0.23 μg ASC mg-1 dw. Antioxidant activities of ABTS ranged from 34.5 ± 0.70 to 156.5 ± 3.06 μg Trolox mg-1 dw. ABTS values showed significant positive correlation (r=0.954**) with DPPH activity. Group I (8 accessions) having high antioxidant activities were obtained by the matrix of calculated distances of TPC and antioxidant activities. K185841, K185879, and K185863 among group I germplasm had higher TPC and antioxidant activities than other accessions. These genotypes are potential sources of natural antioxidants. Therefore, this study provides valuable information for safflower breeders and growers to develop new varieties and produce functional foods.