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자초(紫草) 열수추출물이 각질형성세포 HaCaT의 세포 연접 관련 유전자의 발현에 미치는 영향 연구
조남준 ( Namjoon Cho ),이병권 ( Byeongkwon Lee ),이웅희 ( Woonghee Lee ),김기광 ( Keekwang Kim ),김균언 ( Kyoon Eon Kim ),한효상 ( Hyosang Han ) 대한본초학회 2017 大韓本草學會誌 Vol.32 No.3
Objectives : The aim of this research was to determine the diverse effects of Lithospermi Radix Water Extract (LR) on human keratinocyte HaCaT cells, and to examine whether those effects could be applied to the human skin. Methods : We examined effect of LR on the cell viability of using the MTS assay in human keratinocyte HaCaT cells. The antioxidation effect of LR was analyzed relative to the well-known antioxidant resveratrol, using an ABTS assay. Quantitative RT-PCR analysis revealed that, in HaCaT cells, LR influenced the mRNA expression of tight-junction genes associated with skin moisturization. Furthermore, a wound-healing assay demonstrated altered cell migration in LR-treated HaCaT cells. Result : The cytotoxicity was confirmed to be higher in LR at a concentration of 800 ㎍/㎖ using the MTS assay in HaCaT cells. In comparison to 100 μM resveratrol, 1,600 ㎍/㎖ LR showed either a similar or superior antioxidation effect. LR treatment in HaCaT cells reduced the mRNA expression levels of claudin 3, claudin 4, claudin 6, claudin 8, and ZO-2 to less than 0.80-fold, whereas JAM-A and Tricellulin mRNA expression level increased more than 1.33- fold. In addition, HaCaT cells migration was decreased to 83.9% by LR treatment. Conclusions : LR of antioxidation activity will have an anti-aging effect on the skin by reducing oxidative stress. Further studies are required to address the implications for human skin, given LR`s effects of altering mRNA expression of tight junction-related gene and decreasing cell migration of HaCaT cells.
안면인식 안면인식(Face Recognition) 을 활용한 진도관리 자동화
김남준 ( Namjoon Kim ),정영수 ( Youngsoo Jung ) 한국건축시공학회 2013 한국건축시공학회 학술발표대회 논문집 Vol.13 No.1
Progress measurement is one of key tasks for cost and schedule management in construction management. However, there has been shortage of effort to implement for automated progress measurement. This paper proposes to use a face recognition (FR) technology for automated progress measurement and management in construction sites. The FR technology can acquire detailed progress data that includes the in and out information of labors by location. The expected effectiveness of the proposed system is to provide accurate labor information for progress measurement by utilizing automated data acquisition technology (DAT) with high-speed reading face. In addition, it could be applied to real-time safety and quality management by using the camera images.
Rbfox family proteins make the homo- and hetero-oligomeric complexes
Choi, Sunkyung,Kim, Yong-Eun,Kim, Jae Whan,Cho, Namjoon,Cheon, Seonghye,Kim, Kee K. Academic Press 2018 Biochemical and biophysical research communication Vol. No.
<P><B>Abstract</B></P> <P>Rbfox family of proteins that consists of Rbfox1, Rbfox2, and Rbfox3 in mammals regulates alternative pre-mRNA splicing in various tissues via direct binding to their RNA binding element. Although many studies have indicated the splicing activity of each member of the Rbfox family, the interactions of Rbfox family proteins are largely unknown. Here, we have investigated interactions among Rbfox family proteins. Co-immunoprecipitation (Co-IP) and GST-pull down assays confirmed that Rbfox proteins form homo and hetero complexes. Moreover, <I>in vivo</I> crosslinking using disuccinimidyl suberate treatment indicated that the Rbfox proteins form a dimer which then assembles with other proteins to form a large multiprotein complex. Duolink <I>in situ</I> proximity ligation (PLA) assay revealed that neuron specific Rbfox3 protein interacts with other Rbfox family proteins. This study is the first to provide an evidence that Rbfox family proteins form homo- and hetero-oligomeric complexes <I>in vivo</I>.</P>
김재환,Cho Namjoon,Kim Eun-Mi,Park Ki-Sun,Kang Yeon Woo,Nam Joong Hyeon,Nam Myoung Soo,Kim Kee K. 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.3
Dysfunction of tight junctions and their components can cause diverse skin diseases. Here, we investigated the expression of claudin 1, a major tight junction protein, and changes of tight junction capacity upon treatment of the extracts of Cudrania tricuspidata (C. tricuspidata) and its components, chlorogenic acid, kaempferol, and quercetin. The effects of ethanol extracts of C. tricuspidata (EECT) and water extracts of C. tricuspidata (WECT) on the viability of human keratinocyte HaCaT cells were assessed by cell proliferation assay. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was conducted to measure the expression of claudin 1 mRNA. The protein expression of claudin 1 was analyzed by western blot and its tight junctional distribution was observed with immunofluorescence microscopy analysis. The tight junction capacity was analyzed by dispase assay. Upon treatment of WECT to HaCaT cells, the mRNA and protein expressions of claudin 1 were increased. In addition, chlorogenic acid, kaempferol, and quercetin increased claudin 1 protein expression levels in a dose-dependent manner. WECT and these three compounds enhanced the tight junction capacity of HaCaT cells in dispase assay. WECT, and its components, such as chlorogenic acid, kaempferol, and quercetin, upregulates both mRNA and protein expressions of claudin 1, which leads to the enhancement of tight junction capacity. Thus, WECT could be a therapeutic approach for treating tight junction-disrupted conditions such as atopic dermatitis and psoriasis.
Youngjin Choi,Namjoon Kim,김성현,Seunho Jung 대한화학회 2003 Bulletin of the Korean Chemical Society Vol.24 No.4
In a mediator-aided microbial fuel cell, the choice of a proper mediator is one of the most important factors for the development of a better fuel cell system as it transfers electrons from bacteria to the electrode. The electrochemical behaviors within the lipid layer of two representative mediators, thionin and safranine O both of which exhibit reversible electron transfer reactions, were compared with the fuel cell efficiency. Thionin was found to be much more effective than safranine O though it has lower negative formal potential. Cyclic voltammetric and fluorescence spectroscopic analyses indicated that both mediators easily penetrated the lipid layer to pick up the electrons produced inside bacteria. While thionin could pass through the lipid layer, the gradual accumulation of safranine O was observed within the layer. This restricted dynamic behavior of safranine O led to the poor fuel cell operation despite its good negative formal potential.