cDNA Cloning and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes-Jocheon

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

Fungi belonging to the Paecilomyces spp. have recently been used as food and herbal medicines in Korea and are greatly popular as commercially available powdered supplement or dried fruiting body. Despite this acceptance and its use, little is known of the genes related to its reactive agents. Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces spp. based on the previous identification of ITS1 and ITS2 at the molecular level and collected from Jocheon Miryang, Korea. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes- Jocheon was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDH is comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA. More investigation works including gene expression, immunological analysis etc. will be carried continuously without hesitation after this presentation.

Molecular Cloning, Expression and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes Jocheon-1

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces tenuipes Jocheon-1. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. Paecilomyces tenuipes Jocheon-1 cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes Jocheon-1 was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDHis comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The cDNA encoding Pt-GAPDH was expressed as a 37 kDa polypeptide in baculovirus-infected insect Sf9 cells. The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA.

일부 직장인의 정기 건강진단 실시 결과에 관한 고찰

남택승,홍종관,박종안,한구웅,김주자 순천향대학교 1987 논문집 Vol.10 No.2

서울, 천안, 구미에 각각 위치한 순천향대학 부속병원에서 서울, 천안, 구미 및 인근 군단위 지역의 공무원 및 사립학교 교직원을 대상으로 1986년 4월부터 동년 7월까지 집단건강진단을 실시하고 설문지를 배부, 조사하여 분석한 결과 다음과 같은 성적을 얻었다. 1. 1차 검사결과 유소견율은 수축기혈압 1.1%(남자 1.4%, 여자 0.2%), 이완기혈압 6.4%(남자 8.0%, 여자 1.5%)이었다. 2. 공복시혈당은 전대상자의 3.3%(남자 3.8%, 여자 1.2%)가 고혈당의 유소견율을 나타내었으며 뇨당은 1.3%(남자 1.6%, 여자 0.5%)의 유소견율을 보였다. 3. 순환기계질환에 관한 2차 검사유소견율은 수축기혈압 24.1%, 이완기혈압 74.9%.안저검사 41.1%, 심전도 검사 36.1%. 혈중 트리글리세라이드 검사 36.1%이었다. 4. 혈압의 연도별 변화는 '86년도의 혈압 평균치는 수축기혈압이 남자 122.7±15.7mmHg, 여자 113.0±13.1mmHg로 '82. '84년도에 비해 유의한 차가 있는 것으로 나타났으며(남 P<0.01, 여 P<0.05) 이완기혈압은 남자 80.9±15.1mmHg, 여자 73.8±23.9mmHg로 남자에 있어서만 (P<0.05). 연도별로 유의한 차가 있는 것으로 나타났다. 5. 혈압의 개인별 경시적 변화를 보면 수축기혈압의 경우 전체적으로 99.8%가 계속 정상으로 유지되고 1.2%는 고혈압 상태로 계속 유지되고 있는 것으로 나타났으며 이완기혈압의 경우는 전체적으로 92.8%가 계속 정상, 7.1%가 계속 고혈압으로 나타났다. 6. 혈중 콜레스테롤 평균치의 연도별 변화는 통계적으로 유의하게 증가되었다.(P<0.01). 7. 혈압과 관련요소들에 대한 상관관계에서 상관성이 있는 것은 수축기혈압의 경우 남자에 있어서 비만도, 뇨단백, 혈중 크레아티닌 및 콜레스테롤, 안저검사, 심전도검사, 혈중 트리글리세라이드이었고 여자에 있어서는 Stout지수, Rohrer지수, 뇨단백, 혈중 콜레스테롤, 안저검사, 심전도검사, 혈중 트리글리세라이드이었다. 이완기혈압의 경우 남자에 있어서는 뇨단백, 혈중 콜레스테롤, 안저검사, 심전도 검사, 혈중 트리글리세라이드이었고 여자에 있어서는 Stout지수, Rohrer지수, 뇨단백, 혈중 크레아티닌, 심전도 검사, 혈중 트리글리세라이드이었다. 8. 당뇨질환에 관한 2차 검사 유소견율은 공복시 혈당이 전체 51.8%, 식후 2시간 혈당치가 전체 41.2%이었다. 9. 공복시 혈당 평균치의 연도별 변화에서 '86년도 혈당 평균치는 남자 92.5±20.4㎎%, 여자 87.6±15.9㎎%이었고 연도별 증가는 통계적으로 유의하였다(P<0.01). 10. 혈당의 개인별 경시적 변화는 전체적으로 99.6%가 계속 정상, 2.32%가 계속 고혈당이었고 고혈당에서 정상으로, 정상에서 고혈당으로 된 경우는 각각 0.02%, 0.05%였다. 11. 혈당과 관련요소들에 대한 상관관계에서 상관성이 있는 것은 남녀 모두에서 Stout지수, Rohrer지수, 뇨당, 식후 2시간 혈당치이었다. 12. 건강행태에 관한 설문조사에서 자신의 건강상태에 대한 인식, 흡연, 음주, 식사에 대한 항목이 정상군과 유소견군간에 유의한 차가 없는 것으로 나타났다. Health Insurance Corporation which covers government officials and privated school faculties has held periodic health examination for the insured every other years since 1982 under the Insurance Law. The purpose is to detect diseases and trea diseases at their early stage. Three Soonchunhyang University Affiliated Hospitals located each in Seoul, Chunan, and Gumi city are committed to carry out the health examination. There are two steps in examination. The first step is for all insured, the second step is for the people whose results come out abnormal in the first step examination. Each item of the tests has been predetermined by the Corporation and most of them are to detect many chronic diseases e.g. hypertension, diabetes mellitus, liver disease, pulmonary Tbc., renal disease, and venereal disease. In spite of increasing concern regarding these diseases recently, we are lack of even basic statistics such as prevalence rate. Authors collected health examination results from three hospitals and analysed them.

졸-겔 법으로 증착한 SrBi_(2)Ta_(2)O_(9) 박막의 강유전체 특성

김광수,이남희,이은구 조선대학교 생산기술연구소 2000 生産技術硏究 Vol.22 No.1

Ferroelectric SrB_12Ta_2O_9 (SBT) thin films on Pt/Ti/SiO_2 electrode were fabricated using sol-gel method SBT thin films crystallized from 650℃however, good hysteresis loop can be obtained in the film annealed above 700℃. Pt/T_1O_2/SiO_2 and Pt/SiO_2 electrodes were also used to investigate the Pt electrode dependence of SBT thin films SBT thin films showed random oriented polycrystalline structure and similar morphology regardless of electrodes with quite different surface morphology A 0 2 pm thick SBT film annealed at 750℃ exhibited the remanent polarization(2P_1) of 8μC/㎠ and coercive voltage(V_(c)) of 1V at an applied voltage of 5V

옥수수 播種期와 地上部形質에 관한 硏究

金鎭淇,崔京求,白南赫,陳星桂 全北大學校 1982 論文集 Vol.24 No.-

全北 平野地인 裡里地方에서 옥수수의 播種期와 地上部生育 및 種實收量과의 관계를 調査하여 다음과 같은 결과를 얻었다. 1. 播種期의 早晩이 莖葉의 生育 및 種實收量에 크게 영향을 끼치며 播種이 늦어질수록 出雄, 出絲, 生育日數가 짧아지고 地上部生育은 떨어지는 경향이다. 2. 莖葉生育은 4月 20日 播種을 基點으로, 種實收量은 5月 5日 播種을 基點으로 各各 減少되었다. 3. 地上部諸形質과 種實收量간에는 高度의 正의 相關이 인정되며 種實增收를 위하여는 莖葉生育은 크게 할 필요가 있다. Some results were gained through the experiment that was carried out with a single cross cultivar, Suweon 19, of maize (Zea mays L.) in Iri, the Jeonbug plain area, in 1980, to check relationships between growth characters at different sowing dates. 1. The foliage growth and grain yield were greatly influenced with planting season. The number of days to tasseling, silking, or maturing shortened and the growth of maize decreased as sowing was delayed. 2. The threshold sowing date for foliage production seemed to be April 20 and that for grain yield, May 5. 3. Highly significant positive correlation existed between vegetative growth and grain yield. This may mean that increasing vegetative growth is prerequisite to better grain yield.

연폭로여성근로자들에서의 생물학적 연폭로 지표들의 상호관계

남택승,한구웅,김형아,김정만,이광묵 가톨릭대학 산업의학쎈타 산업의학연구소 1986 韓國의 産業醫學 Vol.25 No.3

For the purpose of obtaining of an effective biological monitoring data in terms of evaluation the health consequences of lead absorption, the female workers in the electronic industry who have been exposed to low-level lead occupationally were chosen as the subjects of our studies. We observed the 9 parameters in not only 305 lead-exposed female workers who have been exposed to lead occupationally also 254 normal female subjects who have not been exposed to lead occupationally. The parameters chosen for this study were as follows, blood lead (PbB), urine lead (PbU), δ-aminolevulinic acid in urine(ALAU), coproporphyrin in urine(CPU), zinc protoporphyrin in whole blood(ZPP), hemoglobin(Hb), hematocrit(Ht), δ-aminolevulinic acid dehydratase(ALAD) activity & ALAD activity ratio(A/R; absorbance at substrate pH 6.6/absorbance at substrate pH 5.8). The results obtained were as follows: 1. The average age of the lead-exposed female workers in the electronic industry and in the normal female subjects were 20.5±4.2 years and 21.7±3.5 years, respectively. 2. The average work duration of the lead-exposed female workers in the electronic industry was 26.4±19.4 months 3. The mean value of PbB of the lead-exposed female workers in the electronic industry and in the normal female subjects were 30.18±6.64㎍/100ml and 21.61±3.84㎍/100ml respectively. 4. In the lead-exposed female workers of the electronic industry and of the normal female subjects, the mean value of ZPP were 36.22±0.69㎍/100ml and 24.64±7.21㎍/100ml, respectively 5. In the lead-exposed female workers of the electronic industry and of the normal female subjects, the mean value of Hb were 13.98±0.57g/100ml and 14.09±0.66g/100ml, respectively. 6. The mean value of the lead-exposed female workers in the electronic industry were 55.07±17.61㎍/1 for PbU, 1.93±0.69mg/1 for ALAU, and 49.74±22.79㎍/1 for CPU, while in the normal female subjects, 44.27±15.76㎍/1 for PbU, 1.46±0.55mg/ 1 for ALAU, and 38.66±17.99㎍/1 for CPU. 7. Among various pairing of the parameters of lead exposure in the female lead-exposed workers and in the normal female subjects, the correlation between PbB and ZPP showed the highest coefficient value.

친환경농업 시범마을에 대한 환경영향평가

이남종,고병구,노기안,한민수,김민경,곽한강,박문희 한국환경농학회 2003 한국환경농학회지 Vol.22 No.4

1999년부터 2001년까지 3년간 친환경농업 시범마을에 적용되고 있는 INM (Integrated Nutrient Management) and IPM (Integrated Pest Management) 실천에 따른 추진효과를 분석하기 위하여 시범마을로 지정된 옥천과 양평지역의 농가 포장에서 시비량, 양분유실 및 생태계 변화 등을 조사 분석하였다. 3요소 시비량은 토양검정시비로 인하여 농가관행시비와 비교하여 28.6~39.4% 절감할 수 있었으며, 쌀 수량도 시비량이 비교적 적은 토양검정 시비구에서 3~10% 증수하였다. 영농기간동안 토양 침투수중 NO₃-N 농도는 농가관행 시비구 2.9 ㎎/L에 비하여 토양검정 시비구는 1.5 ㎎/L로 감소하였으며, 시범마을 논으로 유입되는 관개수의 COD, NH₄-N, NO₃-N 농도는 각각 15.0, 0.67, 1.39 ㎎/L, 시범마을 논에서 배출된 용수는 각각 12.4, 0.29, 2.42 ㎎g/L로 감소되는 경향이었다. 처리별 수서곤충, 환형동물, 패류 및 감각류 등의 개체수는 농가관행구 100에 대하여 토양검정 시비구 293, 토양검정과 개량제 혼용구 276, 무비구 90으로 토양검정 시비구에서 많았으며 수서 무척추 동물은 관행농업을 수행하고 있는 지역을 100으로 볼 때 친환경농업마을은 242와 443으로 INM과 IPM을 실천하는 시범마을에서 생물종이 다양하고 그 개체수가 많은 것으로 나타났다. 친환경농업 시범마을 조성 후 작물 추천시비량 준수와 적기 예찰을 통하여 필요이상 사용되는 비료와 농약을 절감하여 생태계의 개선과 토양이 건전화된 것으로 평가되었다. In order to preserve the soundness of ago-ecosystem and products safety in agriculture, this study was evaluated environmental impact in relation to the INM (Integrated Nutrient Management) and IPM (Integrated Pest Management) at paddy fields at Ok-Chun and Yang-Pyung region. By introduction of INM with the application of BB(bulk-blanded) fertilizer based on soil analysis and EM, the application rate of fertilizer was reduced to about 28.6-39.4% and the yield of brown rice was increased to about 3-10% compared to conventional practices. The concentrations of COD, NH4-N, and NO3-N in irrigation water flowed to the environmental-friendly agriculture practices were 15.0, 0.67, and 1.39 ㎎/L, respectively. The concentrations of COD, NH₄-N, and NO₃-N from paddy fields in drainage water were 12.4, 0.29, and 2.42 ㎎/L, respectively. The total number of the freshwater invertebrates was higher in field treated with fektilhtion by prescription with soil testing. Also, the population density of aquatic insects was higher than the other fields at both demonstration villages. In conclusion, it was possible to reduce the amount of fertilizer and agricultural chemicals application, and increase the yield of rice by application of the environmental-friendly agriculture practices.

麥酒麥의 (1-3,1-4)-β-glucanase 遺傳子의 構造와 發現 樣狀

유남희,윤성중,최경구,김제환,박충웅 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

禾穀類 種子 發芽時 호분층과 ??組織의 細胞壁에 多量 含有되어 있는 (1-3, 1-4)-β-glucan을 分解하여 種子의 發芽에 必要한 加水分解酵素의 分泌 및 擴散 浸透를 돕는다. 本 硏究에서는 國內 麥酒麥의 (1-3, 1-4)-β-glucanase 遺傳子의 構造 및 發現 에 대한 基礎 情報를 얻기 위하여 生化學的 및 分子生物學的 方法을 利用하여 發芽種子 및 어린 잎에서의 酵素活性과 遺傳子의 發芽樣狀 그리고 遺傳子의 構造 等을 調査하였다. 麥酒麥 發芽種子中 (1-3, 1-4)-β-glucanase 活性은 發芽 7日째에 제일 높았다. (1-3, 1-4)-β-glucanase의 活性測定에 使用한 (1-3, 1-4)-β-glucan이 (1-4)-β-glucanase에 의해 分解될 可能性이 있으므로 (1-4)-β-glucanase의 活性도 測定하였는데 (1-4)-β-glucanase의 活性이 매우 낮게 나타나서(1-4)-β-glucanase가 (1-3, 1-4)-β-glucanase을 分解하여 (1-3, 1-4)-β-glucanse의 活性에 影響을 미쳤을 可能性이 매우 낮았다. 麥酒麥의 (1-3, 1-4)-β-glucanase 遺傳子 構造를 PCR을 利用하여 調査하였던바 國內 麥酒麥의 (1-3, 1-4)-β-glucanase 遺傳子 構造는 外國의 다른 品種들이나 귀리의 遺傳子 構造와 대단히 類似하였다. 酵素活性 測定과 귀리의 (1-3, 1-4)-β-glucanase cDNA clone을 ?識子로 利用한 보리의 mRNA 分析에 의해서 發芽傳子 및 어린 잎에서 (1-3, 1-4)-β-glucanase가 種子發芽 以外에도 生育中인 組織細胞의 伸張과 發育 및 β-glucan 再利用에 泌要한 β-glucan 代謝에 關與하고 있음을 示唆하는 結果라 생각된다. (1-3, 1-4)-β-glucans hydrolysis of cell wall (1-3, 1-4)-β-glucanas accompanies degradation of endosperm cell walls during cereal seed germination. To investigate (1-3, 1-4)-β-glucanase expression pattern in Korean malting barley, (1-3, 1-4)-β-glucanase activity, gene structure and mRNA expression patterns werre examined. (1-3, 1-4)-β-glucanase activity increased as germination proceeded and the highest enzyme activity was detected in seeds germinated for 7 days. Because (1-4)-β-glucanase have a potential to degrade (1-3, 1-4)-β-glucans, (1-4)-β-glucanase activity was also determined. (1-4)-β-glucanase activity, however, was very low minimizing possibility of (1-4)-β-glucanase action on (1-3, 1-4)-β-glucan substrate. PCR technique was employed to investigate (1-3, 1-4)-β-glucanase gene structure. The amplification product of the expected size from the targeted sites of Dusan #29 indicated that (1-3, 1-4)-β-glucanase systems were conserved in different barley varieties and even in oats. (1-3, 1-4)-β-glucanase activity and mRNAs were detected in young leaves as well as in germinating seeds. This may suggest that (1-3, 1-4)-β-glucanase are also involved in β-glucan metabolism required for cell elongation and β-glucan reutilization during leaf growth and developmetn.

산불 피해 소나무재의 역학적 특성 및 급속오븐 건조특성

황원중,권구중,박종수,김남훈 한국목재공학회 2003 목재공학 Vol.31 No.4

본 연구에서는 산불 피해 소나무재의 이용을 위한 기초자료를 얻고자 역학적 및 건조 특성을 조사하였다. 산불피해재는 종압축 특성, 전단강도, 충격휨흡수에너지 등에서 건전재와 거의 유사한 결과를 보여주었으며, 산불피해재 변재부의 휨특성은 건전재보다 양호하게 나타났다. 건전재와 산불피해재 변재부의 생재함수율은 산불피해재가 다소 낮았으나, 건조속도는 거의 차이가 없었다. 건조시 발생되는 초기할렬은 건전재가 산불피해재보다 발생율이 높았고, 내부할렬은 건전재와 산불피해재에서 발생되지 않았다. 따라서 산불에 의해 피해를 입은 목재의 대부분은 수피부를 제거하여 이용할 경우 건전재와 거의 동일한 용도로서 사용이 가능할 것으로 생각된다. The Mechanical and drying characteristics of Pinus densiflora wood, which was damaged by forest fire, and sound wood were examined. There were no significant differences in axial compressive properties, shearing strength and impact bending absorbed energy between damaged and sound woods. But the bending properties of damaged wood were slightly higher than those of sound wood. The green moisture content of sapwood in damaged wood was lower than that in sound wood, but oven-drying rate was similar in both woods. Number of initial check in damaged wood was smaller than that in sound wood, and inner check in damaged and sound woods was not observed. Consequently, it is considered that mostly damaged wood by forest fire can be used for wood material after removing the considered that mostly damaged wood by forest fire can be used for wood material after removing the bark.

다변량해석기법을 이용한 만경강水系의 水質評價

오연찬,이남도,김종구 한국환경과학회 2004 한국환경과학회지 Vol.13 No.3

This study was conducted to evaluate water quality in the Mankyung River using multivariate analysis. The analysis data which was surveyed from January 1996 to December 2002 in Mankyung river was aquired by the ministry of environment. Twelve water quality parameters were determined on each survey. The results were summarized as follow ; Water quality in the Mankyung River could be explained up to 74.90% by four factors which were included in loading of organic matter and nutrients by the tributaries(43.28%), seasonal variation(10.40%), loading of pathogenic bacteria by domestic sewage of Gapcheon (12.41%) and internal metabolism in river(8.81%). The result of cluster analysis by station was classified into three group that has different water quality characteristics. Especially, Iksan river was appeared to considerable water quality characteristics against other station. In monthly cluster analysis, three group was classified by seasonal characteristics. Also, in yearly cluster analysis, three group was classified. It is necessary to control the pollutant loadings by domestic sewage and livestock waste for water quality management of Mankyung river.