종양과 구강각질세포주에서 Celecoxib과 Indomethacin의 Cyclooxygenase-2 비의존적 기전에 의한 억제 효과

노종렬,성명훈,김광현,김동영,하정훈,박찬일 충남대학교 암공동연구소 2005 암공동연구소 업적집 Vol.4 No.

Background and Objectives : Overexpression of cyclooxygenase-2 (COX-2), a rate-limiting enyme in the generation of prostanoids from arachidonic acid, has known to be closely related to tumorigenesis, tumor growth, angiogenesis, and metastasis. Selective or non-selective COX-2 inhibitors have been used for the growth inhibition of cancers with preventative intents ; however, it has been suggested recently that cancer cells have COX-2-independent mechanisms. Materials and Method : Using MTT assat and cell counts, we observed the growth inhibition of SCC VII, CT-26 and B16F10 murine cancer cell lines when treated by celecoxib and indomethacin. SNU-1041 and HOK 16B were used as controls for comparing with the murine cell lines. The COX-2 expression of these cell lines was analyzed by western blotting and compared with the degree of inhibition by the drugs. Results : The growth inhibition of the cell lines by the drugs was clearly demonstrated in a concentration-dependent manner and depended on the type of cell lines and test drug. The in vitro viability assay revealed that CT-26 expressing COX-2 protein was slightly inhibited but SCC Vn and B16F10 without COX-2 expression were moderate-to-highly inhibited by the drug treatment. Celecoxib and indomethacin appeared to have no close relation with the COX-2 expression of cell lines in their growth inhibition, HOK 16B showed a resistance by concentrations less than 25 μM of celecoxib, which implies that celecoxib has a more selective effect on tumor cells and is safer than indomethacin Conclusion : The growth of cancer cells was inhibited by celecoxib and indomethacin treatment, which depends on the type of cancer, treated drug, and its concentration. Their suppressive effect is not closely related to the COX-2 expression of cancer cells. (Korean J Otolaryngol 2004;47:562-8)

종양과 구강각질세포주에서 Celecoxib과 Indomethacin의 Cyclooxygenase-2 비의존적 기전에 의한 억제 효과

노종렬,성명훈,김광현,김동영,하정훈,박찬일 충남대학교 암연구소 2005 암연구소 업적집 Vol.4 No.-

Background and Objectives : Overexpression of cyclooxygenase-2 (COX-2), a rate-limiting enyme in the generation of prostanoids from arachidonic acid, has known to be closely related to tumorigenesis, tumor growth, angiogenesis, and metastasis. Selective or non-selective COX-2 inhibitors have been used for the growth inhibition of cancers with preventative intents ; however, it has been suggested recently that cancer cells have COX-2-independent mechanisms. Materials and Method : Using MTT assat and cell counts, we observed the growth inhibition of SCC VII, CT-26 and B16F10 murine cancer cell lines when treated by celecoxib and indomethacin. SNU-1041 and HOK 16B were used as controls for comparing with the murine cell lines. The COX-2 expression of these cell lines was analyzed by western blotting and compared with the degree of inhibition by the drugs. Results : The growth inhibition of the cell lines by the drugs was clearly demonstrated in a concentration-dependent manner and depended on the type of cell lines and test drug. The in vitro viability assay revealed that CT-26 expressing COX-2 protein was slightly inhibited but SCC Vn and B16F10 without COX-2 expression were moderate-to-highly inhibited by the drug treatment. Celecoxib and indomethacin appeared to have no close relation with the COX-2 expression of cell lines in their growth inhibition, HOK 16B showed a resistance by concentrations less than 25 μM of celecoxib, which implies that celecoxib has a more selective effect on tumor cells and is safer than indomethacin Conclusion : The growth of cancer cells was inhibited by celecoxib and indomethacin treatment, which depends on the type of cancer, treated drug, and its concentration. Their suppressive effect is not closely related to the COX-2 expression of cancer cells. (Korean J Otolaryngol 2004;47:562-8)

구강내에서 재광화용액 "R"의 법랑질 초기 우식병소에 대한 재광화의 정량적 평가

김명은,정일영,금기연,이찬영,노병덕 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.2

Dental caries is a chronic disease that causes the destruction of tooth structure by the interaction of plaque bacteria, food debris, and saliva. There has been attempts to induce remineralization by supersaturating the intra-oral environment around the surface enamel, where there is incipient caries. In this study, supersaturated remineralized solution "R" was applied to specimens with incipient enamel caries, and the quantitative ananlysis of remineralization was evaluated using microradiography. Thirty subjects volunteered to participate in this study. Removable appliances were constructed for the subjects. and the enamel specimen with incipient caries were embedded in the appliances. The subjects wore the intra-oral appliance for 15 days except while eating and sleeping. The removable appliance were soaked in supersaturated solution "R", saline, or Senstime® to expose the specimen to those solutions three times a day, 5 minutes each time. After 15 days, microradiography was retaken to compare and evaluate remineralization. The results were as the following: 1. The ratio of remineralized area to demineralized area was significantly higher in the supersaturated solution "R" and Senstime® than in the saline (p<0.05) 2. Remineralization in the supersaturated buffer solution "R" occurred in the significantly deeper parts of the tooth, compared to the Senstime® group containing high concentration of fluoride.(p<0.05) As in the above results, the remineralization effect of remineralized buffer solution "R" on incipient enamel caries has been proven. For clinical utilization, further studies on soft tissue reaction and the effect on dentin and cementum are necessary. In conclusion compared to commercially available fluoride solution, remineralization solution "R" showed better remineralization effect on early enamel caries lesion. so it is considered as effecient solution for clinical application

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

Block layout method in the block stockyard based on the genetic algorithm

Roh, Myung-Il Techno-Press 2012 Ocean systems engineering Vol.2 No.4

Due to its large size, a ship is first divided into scores of blocks and then each block is constructed through various shops, such as the assembly shop, the painting shop, and the outfitting shop. However, each block may not be directly moved to the next shop and may be temporarily laid at a block stockyard because the working time in each shop is different from each other. If blocks are laid at the block stockyard without any planning, the rearrangement of the blocks by a transporter is required because the blocks have the different in and out time. In this study, a block layout method based on the genetic algorithm was proposed in order to minimize the rearrangement of the blocks in the block stockyard. To evaluate the applicability of the proposed method, it was applied to simple layout problems of the block stockyard. The result shows that the proposed method can yield a block layout that minimizes the total relocation cost of moving obstacle blocks in the block stockyard.

Determination of an economical shipping route considering the effects of sea state for lower fuel consumption

Myung-Il Roh 대한조선학회 2013 International Journal of Naval Architecture and Oc Vol.5 No.2

With increases in international oil prices, the proportion of fuel cost to the operational costs of a ship is currently increasing. To reduce fuel cost, a method for determining an economical route for a ship based on the acquisition of the sea state and the estimation of fuel consumption is proposed. The proposed method consists of three items. The first item is to acquire the sea state information in real time. The second item is to estimate the fuel consumption of a ship according to the sea state. The last item is to find an economical route for minimal fuel consumption based on the previous two items. To evaluate the applicability of the proposed method, it was applied to routing problems in various ocean areas. The result shows that the proposed method can yield economical ship routes that minimize fuel consumption. The results of this study can contribute to energy savings for environmentally friendly ships.

Generation of production material information for a building block and simulation of block erection for process planning and scheduling in shipbuilding

Roh, Myung-Il,Lee, Kyu-Yeul Taylor Francis 2007 INTERNATIONAL JOURNAL OF PRODUCTION RESEARCH - Vol.45 No.20

<P> At the initial process planning and scheduling stage of a ship, the generation task of the production material information of a building block and the simulation task of the block erection have been manually performed using 2D drawings, etc. To make these tasks automatic, an accurate generation method for the production material information and a convenient simulation method for block erection using a 3D CAD model are developed in this study. For this, a 3D CAD model for a whole hull structure is generated first, and the block division method for dividing the 3D CAD model into a number of building blocks is then developed using the relationship between the hull structural parts. A generation method for the production material information for calculating the weight, etc., of a building block is developed. A simulation method for block erection is also developed using the composition and erection sequence information of erection blocks. Finally, to evaluate the developed methods, these methods are applied to corresponding processes of a deadweight 300 000-ton very large crude oil carrier. As a result, it is shown that the production material information can be accurately generated and the block erection can be conveniently simulated at the initial design stage.</P>

Determination of an economical shipping route considering the effects of sea state for lower fuel consumption

Roh, Myung-Il The Society of Naval Architects of Korea 2013 International Journal of Naval Architecture and Oc Vol.5 No.2

With increases in international oil prices, the proportion of fuel cost to the operational costs of a ship is currently increasing. To reduce fuel cost, a method for determining an economical route for a ship based on the acquisition of the sea state and the estimation of fuel consumption is proposed. The proposed method consists of three items. The first item is to acquire the sea state information in real time. The second item is to estimate the fuel consumption of a ship according to the sea state. The last item is to find an economical route for minimal fuel consumption based on the previous two items. To evaluate the applicability of the proposed method, it was applied to routing problems in various ocean areas. The result shows that the proposed method can yield economical ship routes that minimize fuel consumption. The results of this study can contribute to energy savings for environmentally friendly ships.

An Algorithm for Generating' the Hull Structural Analysis Model Using the Seam Information of the Hull Structure at the Initial Design Stage

Roh, Myung-Il,Lee, Kyu-Yeul,Yoo, Seong-Jin The Society of Naval Architects of Korea 2006 Journal of ship and ocean technology Vol.10 No.4

So far, the generation of a hull structural analysis model, that is, a finite element model of a hull structure, has been manually performed by a designer using design experience, and thus has required lots of time because of many constraints, the complexity, and the huge size of the hull structure. To make this task automatic, an algorithm for generating the hull structural analysis model is developed using the seam information of the hull structure. A generating system of the hull structural analysis model is implemented based on the developed algorithm. The applicability of the developed algorithm is demonstrated by applying it to the generation of the global and hold structural analysis models of a deadweight 300,000 ton VLCC (Very Large Crude oil Carrier). The results show that the developed algorithm can quickly generate these models at the initial design stage.

초기 설계를 위한 자료 구조 및 모델링 함수 기반의 선체 구조 CAD 시스템 개발

노명일(Myung-Il Roh),이규열(Kyu-Yeul Lee) 대한조선학회 2006 大韓造船學會 論文集 Vol.43 No.3