Serum MicroRNA Expression Profiling in Mice Infected with Rabies Virus

Han, Myung Guk,Park, Jun-Sun,Lee, Cho Soon,Jeong, Young Eui,Ryou, Jung Sang,Cho, Jung Eun,Ju, Young Ran,Lee, Kyoung-Ki Korea Centers for Disease Control and Prevention 2011 Osong Public Health and Research Persptectives Vol.2 No.3

<P><B>Objectives</B></P><P>Serum or plasma microRNAs (miRNAs) are potential biomarkers for the diagnosis for cancer and prenatal diseases. This study was conducted to investigate whether rabies virus causes a change in serum miRNA expression.</P><P><B>Methods</B></P><P>ICR mice were intramuscularly inoculated with rabies virus and were sacrificed weekly to collect serum and brain tissue for 4 weeks postinoculation. Mice were assigned to four groups based on the results of indirect immunofluorescent assays, enzyme-linked immunosorbent assay, and nested reverse transcription-polymerase chain reaction and the expression profiles of serum miRNAs were compared using a commercial mouse miRNA expression profiling assay.</P><P><B>Results</B></P><P>The expression levels of miRNAs changed significantly with the different stages of the disease. The expression level of 94 serum miRNAs in infected mice changed at least twofold. Seven microRNAs of them were significantly upregulated or downregulated in all infected mice regardless of disease status. The number of miRNAs with an expression level change decreased with the progression of the disease. In a hierarchical cluster analysis, infected mice clustered into a group separate from uninfected control mice.</P><P><B>Conclusions</B></P><P>Based on the relationship of miRNAs to gene expression regulation, miRNAs may be candidates for the study of viral pathogenesis and could have potential as biomarkers.</P>

Development of Radiochemical Analysis of Uranium Isotopes in Soil Sampoles with Extraction Chromatography

Myung Ho Lee,Guk Sik Choi,Young Hyun Cho,Chang Woo Lee,Soo Yong Lee 대한방사선방어학회 2001 방사선방어학회지 Vol.26 No.1

An aspect of voiceless stops in word-final coda in American English

( Guk-in Cho ),( Jin-myung Lee ) 현대영미어문학회 2015 현대영미어문학회 추계학술대회 발표논문집 Vol.2015 No.-

세포분리배양에서 신경성장인자와 탈분극이 나선신경절 신경세표의 생존에 미치는 효과

김명순,정원호,조양선,신동봉,방은국,류성희,홍성화 대한이비인후과학회 2001 대한이비인후과학회지 두경부외과학 Vol.44 No.11

실험토끼에서 선택적 신동맥 카테터를 통한 재조합 아데노바이러스 벡터의 새로 개발된 효율적인 주입법

길명철(Myung Cheol Gil),조재욱(Jae Wook Cho),황준승(Jun Seong Hwang),윤성국(Seong Guk Yoon),정재일(Jae Il Chung),정세일(Se Il Jung) 대한비뇨기종양학회 2007 대한비뇨기종양학회지 Vol.5 No.3

Development of Radiochemical Analysis of Uranium Isotopes in Soil Samples with Extraction Chromatography

Lee, Myung Ho,Choi, Guk Sik,Cho, Young Hyun,Lee, Chang Woo,Lee, Soo Yong 대한방사선 방어학회 2001 방사선방어학회지 Vol.26 No.1

방사성 동위원소에 오염된 토양시료에 대하여 정확하고 신속한 U 동위원소 분석법을 개발하였고 IAEA 표준시료에 적용하여 분석법의 타당성을 검증하였다. TBP 용매추출법의 단점을 보완하기 위해서 토양시료를 질산 및 불산을 사용하여 메트릭스 성분으로부터 우라늄을 완벽하게 추출하였고 이온교환수지와 TRU Spec 수지를 이용하여 우라늄 동위원소를 악티나이드 원소로부터 순수분리하였다. 크로마토 그래피 추출법을 사용하여 토양시료에서 우라늄 동위원소 화학수율은 TBP 용매추출법의 경우보다 10% 정도 증가하였다. 크로마토 그래피 추출법을 사용한 IAEA 토양시료에 분석결과는 IAEA에서 보고된 수치와 일치하였다. An accurate and raped analytical technique of uranium isotopes in highly contaminated soil samples was developed and validated by application to the IAEA-Reference samples. For overcoming the demerits of the TBP extraction method, sample materials were decomposited with HNO3 and HF, and uranium isotopes were purified by an anion exchange resin and a TRU Spec resin. With the extraction chromatography method, the hindrance elements were completely removed from the uranium fraction. The chemical yields with the extraction chromatography method were more 10 % higher than those with the TBP extraction method. The concentrations of uranium isotopes in soil samples using the extraction chromatography method were consistent with the reference values reported by the IAEA.

양성 갑상선 결절에서 갑상선 호르몬 억제요법의 치료 효과

김극배,유명희,조영덕,송동화,서교일 대한내분비학회 1991 Endocrinology and metabolism Vol.6 No.2

To evaluate the therapeutic effect of thyroid hormone supressive therapy for benign thyroid nodule and factors affecting suppression of endogenous thyrotropin secretion by thyroid hormone treatment, we measured plasma T3, T4, TSH concentration, total thyroid volume and thyroid nodule volume by sequential ultrasonic tomogram in 19 patients with adenomatous goiter or follicular neoplasm on thyroid needle biopsy and aspiration cytology before and after thyroid hormone suppressive therapy for 3 to 6 months. According to the therapeutic response patients were classified as group Ⅰ (decrease in nodule volume ≥ 50%) and group Ⅱ(decrease in nodule volume $lt; 50%). 1) Before treatment, the thyroid nodule volume showed no significant difference between group Ⅰ (6.01±1.83 ml) and group Ⅱ (5.47±1.78 ml)(p$gt;0.05). After thyroid hormone treatment, thyroid nodule volume became smaller in group Ⅰ (1.05±0.31 ml) than those of group Ⅱ (4.38±1.78 ml)(p$lt;0.05). 2) After thyroid hormone therapy, plasma TSH concentration was on group Ⅰ (0.05±0.02 mU/L) than those of group Ⅱ (0.29±0.15 mU/L), but plasma T3 and T4 concentration showed no significant difference between group Ⅰ and Ⅱ (p$gt;0.05). 3) After treatment, total thyroid volume of group Ⅰ (21.45±2.44 ml) was not significantly different from group Ⅱ (23.33±3.00 ml)(p$gt;0.05). 4) After thyroid hormone therapy, 53%(10/19 patients) of patients showed decrease in nodule volume more than 50%. 5) Out of 5 group Ⅱ patients with the cytologic diagnosis of follicular proliferation, 4 patients underwent surgery revealing 2 patients as follicular carcinoma and 1 patient as papillary carcinoma and 1 patient as follicular adenoma. These findings suggest that thyroid hormone therapy decreased thyroid nodule volume in 53% of patients with benign thyroid nodules more than 50% and the therapeutic effect was influenced by palsma TSH concentration by radioimmunometric assay. Also thyroid nodules which were not decreased after thyroid hormone therapy were highly recommended to surgery, since 3 ou 4 exploration revealed thyroid cancer. (J Kor Soc Endocrinol 6:141~149,1991)

고령 환자의 대퇴 전자간부 불안정성 골절의 치료 : 압박 고 나사를 이용한 내고정술과 시멘트 양극성 반치환술의 결과 비교

박명식 ( Myung Sik Park ),정우철 ( Woo Chul Jung ),박혁 ( Hyuk Park ),황병연 ( Byung Yun Hwang ),임영진 ( Young Jin Lim ),정명국 ( Myung Guk Jung ),조홍만 ( Hong Man Cho ) 대한골절학회 2009 대한골절학회지 Vol.22 No.2

목적: 골다공증을 동반한 고령 환자에서 대퇴 전자간부 불안정성 골절의 압박 고 나사못을 이용한 내고정술과 시멘트 양극성 반치환술을 시행한 집단의 임상적 결과를 비교 분석하고자 하였다. 대상 및 방법: 2001년 1월부터 2006년 10월까지 대퇴 전자간부 불안정성 골절로 수술을 시행한 60세 이상 환자 중 2년 이상 추시가 가능하였던 환자 73예를 대상으로 하였고, 시멘트 양극성 반치환술을 시행한 환자 34예를 A군, 압박 고 나사를 이용한 내고정술을 시행한 환자 39예를 B군으로 분류하여 후향적으로 분석하였다. 수술 중 출혈량 및 수술시간을 분석하였고, 술 후 임상 결과와 합병증을 분석하였다. 결과: 수술 중 출혈량 및 수술 시간 모두 두 군 간 통계학적 유의성은 보이지 않았다. 임상 결과는 일상생활 기능 평가에서 A군에서의 결과가 더 양호하였다. A군에서 고관절 탈구 1예, 대전자부 불유합 1예, 감염 1예, 대퇴스템의 해리 1예를 보였으며, B군에서는 8예에서 고정물 실패와 1예에서 수술 부위 감염을 보였다. 결론: 골다공증을 동반한 고령 환자의 대퇴 전자간부 불안정성 골절 치료 시 시멘트 양극성 반치환술의 단기 추시 결과는 우수하였지만, 인공관절 수명을 고려해본다면 장기간의 추시가 필요하리라 생각한다. Purpose: To perform comparative analysis between the results of internal fixation using compression hip screw and cemented bipolar hemiarthroplasty in unstable intertrochanteric fracture in elderly patients. Materials and Methods: From January 2001 to October 2006, we reviewd 73 patients, who were treated surgically for unstable intertrochanteric fractures, with a minimum of 2 years follow up. The patient`s age was older than 60 year old. The patients were divided into two groups and evaluated, retrospectively. One group was treated with cemented bipolar hemiarthroplasty (Group A, 34 cases), and the other group was treated with compression hip screw (Group B, 39 cases). We evaluated the amount of intraoperative bleeding, operative time, clinical results and complications between the two groups. Results: The amount of intraoperative bleeding and operative time were no statistically significant between the two groups. Group A showed a better result than Group B for clinical outcome using Johnson Daily Activity of Life. Complications in the group A were comprised of dislocation (1 case), nonunion of greater trochanter (1 case), infection (1 case) and loosening (1 case), and those in the group B were comprised of loss of fixation (8 cases) and infection (1 case). Conclusion: We found that short-term outcomes of cemented bipolar hemiarthroplasty for unstable intertrochanteric fractures were satisfactory. However, a longer-follow up period is necessary to clarify the efficacy of cemented bipolar hemiarthroplasty.

고령 환자의 대퇴 전자간부 불안정성 골절의 치료 -압박 고 나사를 이용한 내고정술과 시멘트 양극성 반치환술의 결과 비교

박명식 ( Myung Sik Park ),정우철 ( Woo Chul Jung ),박혁 ( Hyuk Park ),황병연 ( Byung Yun Hwang ),임영진 ( Young Jin Lim ),정명국 ( Myung Guk Jung ),조홍만 ( Hong Man Cho ) 대한골절학회 2009 대한골절학회지 Vol.22 No.3

목적: 골다공증을 동반한 고령 환자에서 대퇴 전자간부 불안정성 골절의 압박 고 나사못을 이용한 내고정술과 시멘트 양극성 반치환술을 시행한 집단의 임상적 결과를 비교 분석하고자 하였다. 대상 및 방법: 2001년 1월부터 2006년 10월까지 대퇴 전자간부 불안정성 골절로 수술을 시행한 60세 이상 환자 중 2년 이상 추시가 가능하였던 환자 73예를 대상으로 하였고, 시멘트 양극성 반치환술을 시행한 환자 34예를 A군, 압박 고 나사를 이용한 내고정술을 시행한 환자 39예를 B군으로 분류하여 후향적으로 분석하였다. 수술 중 출혈량 및 수술시간을 분석하였고, 술 후 임상 결과와 합병증을 분석하였다. 결과: 수술 중 출혈량 및 수술 시간 모두 두 군 간 통계학적 유의성은 보이지 않았다. 임상 결과는 일상생활 기능 평가에서 A군에서의 결과가 더 양호하였다. A군에서 고관절 탈구 1예, 대전자부 불유합 1예, 감염 1예, 대퇴스템의 해리 1예를 보였으며, B군에서는 8예에서 고정물 실패와 1예에서 수술 부위 감염을 보였다. 결론: 골다공증을 동반한 고령 환자의 대퇴 전자간부 불안정성 골절 치료 시 시멘트 양극성 반치환술의 단기 추시 결과는 우수하였지만, 인공관절 수명을 고려해본다면 장기간의 추시가 필요하리라 생각한다. Purpose: To perform comparative analysis between the results of internal fixation using compression hip screw and cemented bipolar hemiarthroplasty in unstable intertrochanteric fracture in elderly patients. Materials and Methods: From January 2001 to October 2006, we reviewd 73 patients, who were treated surgically for unstable intertrochanteric fractures, with a minimum of 2 years follow up. The patient`s age was older than 60 year old. The patients were divided into two groups and evaluated, retrospectively. One group was treated with cemented bipolar hemiarthroplasty (Group A, 34 cases), and the other group was treated with compression hip screw (Group B, 39 cases). We evaluated the amount of intraoperative bleeding, operative time, clinical results and complications between the two groups. Results: The amount of intraoperative bleeding and operative time were no statistically significant between the two groups. Group A showed a better result than Group B for clinical outcome using Johnson Daily Activity of Life. Complications in the group A were comprised of dislocation (1 case), nonunion of greater trochanter (1 case), infection (1 case) and loosening (1 case), and those in the group B were comprised of loss of fixation (8 cases) and infection (1 case). Conclusion: We found that short-term outcomes of cemented bipolar hemiarthroplasty for unstable intertrochanteric fractures were satisfactory. However, a longer-follow up period is necessary to clarify the efficacy of cemented bipolar hemiarthroplasty.

Poster Session : PS 1307 ; Endocrinology : Preoperative Localization and Intraoperative Parathyroid Hormone Assay in Korean Patients with Primary Hyperparathyroidism

( Eirie Cho ),( Min Joo Kim ),( Jung Mi Chang ),( Seok Young Yoon ),( Gil Tae Lee ),( Yun Hyi Ku ),( Hong Il Kim ),( Myung Chul Lee ),( Guk Haeng Lee ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Intraoperative parathyroid hormone (IOPTH) assay is widely used in patients with primary hyperparathyroidism (PHPT). We investigated the usefulness of IOPTH assay in Korean patients with PHPT. Methods: We retrospectively reviewed the data of 33 patients with PHPT who underwent parathyroidectomy. Neck ultrasonography (US) and 99mTc-sestamibi scintigraphy (MIBI scan) were performed preoperatively and IOPTH assays were conducted. Results: The sensitivity of neck US and MIBI scans were 91% and 94%, respectively. A 50% decreased in parathyroid hormone (PTH) levels 10 minutes after excision of the parathyroid gland was obtained in 91% (30/33) of patients and operative success was achieved in 94% (32/33) of patients. The IOPTH assay was 91% true-positive, 3% true-negative, 0% false-positive, and 6% false-negative. The overall accuracy of the IOPTH assay was 94%. In 5 cases with discordant neck US and MIBI scan results, a sufficient decrease in IOPTH levels helped the surgeon confirm the complete excision of the parathyroid gland and perform no additional neck exploration. Conclusions: The IOPTH assay is an accurate tool for localizing hyperfunctioning parathyroid glands and is helpful for evaluating cases with discordant neck US and MIBI scan results.