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- 저자 : Muhammad Faisal Siddiqui (검색결과 5 건)
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Muhammad Faisal Siddiqui,오현석,Miles Rzechowicz,Harvey Winters,Tzyy Haur Chong,Anthony G. Fane 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.30 No.-
Exploring novel biological strategies to mitigate membrane biofouling is of great worth in order to allowsustainable performance of membrane systems for wastewater treatment. Here, the optimal biofilmprevention potential of three phenolic compounds, viz. tannic acid (TA), ellagic acid (EA), andepigallocatechin (EG) on polystyrene microtiter plate, glass surface, and reverse osmosis (RO) membranecoupons was investigated using Pseudomonas aeruginosa PAO1 and RO multispecies community. Biofilmformation was qualitatively and quantitatively assessed by crystal voilet assay and confocal microscopy[bacterial cells and the components of extracellular polymeric substances (EPS)]. The three phenoliccompounds had different optimal concentrations (TA 100 mg/L, EA 200 mg/L, and EG 200 mg/L) forbiofilm control. Biofilm control was correlated with a reduction in EPS. The three phenolic compoundshad no dispersal effect on 24 h-old PAO1 biofilms. Phenolic compounds also reduced multispeciesbiofilm formation of RO community. The data present strong evidence for the application of thesephenolic compounds for the prevention of biofouling in an industrial setting.
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Acute Gastrointestinal Bleeding in COVID-19 Patients: A Systematic Review and Meta-Analysis
Umair Iqbal,Hafsa Anwar,Hafiz Umair Siddiqui,Muhammad Ali Khan,Faisal Kamal,Bradley D. Confer,Harshit S. Khara 대한소화기내시경학회 2021 Clinical Endoscopy Vol.54 No.4
Background/Aims: More than 100 million people to date have been affected by the coronavirus disease-2019 (COVID-19)pandemic. Patients with COVID-19 have a higher risk of bleeding complications. We performed a systematic review and metaanalysisto evaluate the outcomes of COVID-19 patients with signs and symptoms of acute gastrointestinal bleeding (GIB). Methods: A systematic literature search was carried out for articles published until until November 11, 2020, in the Embase,MEDLINE, Web of Science, and Cochrane Library databases. We included studies on COVID-19 patients with signs and symptomsof GIB. Results: Our search yielded 49 studies, of which eight with a collective 127 patients (86 males and 41 females) met our inclusioncriteria. Conservative management alone was performed in 59% of the patients, endoscopic evaluation in 31.5%, and interventionalradiology (IR) embolization in 11%. Peptic ulcer disease was the most common endoscopic finding, diagnosed in 47.5% of thepatients. Pooled overall mortality was 19.1% (95% confidence interval [CI]; 12.7%-27.6%) and pooled mortality secondary to GIBwas 3.5% (95% CI; 1.3%–9.1%). The pooled risk of rebleeding was 11.3% (95% CI; 6.8%–18.4%). Conclusions: The majority of COVID-19 patients with GIB responded to conservative management, with a low mortalityrate associated with GIB and the risk of rebleeding. Thus, we suggest limiting endoscopic and IR interventions to those withhemodynamic instability and those for whom conservative management was unsuccessful.
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Biohydrogen production from palm oil mill effluent using immobilized mixed culture
Lakhveer Singh,Zularisam A. Wahid,Muhammad Faisal Siddiqui,Anwer Ahmad,Mohd Hasbi Ab. Rahim,Mimi Sakinah 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.2
Cell immobilization techniques were adopted to bio-hydrogen production using immobilized anaerobic sludge as the seed culture. Palm oil mill effluent (POME) was used as the substrate carbon source. It was found that with a POME concentration of 20 g COD/l in the feed, the suspended-cell containing reactor was able to produce hydrogen at an optimal rate of 0.348 l H2/(l POME h) at HRT 6 h. However, the immobilized-cell containing reactor exhibited a better hydrogen production rate of 0.589 l H2/(l POME h), which occurred at HRT 2 h. When the immobilized-cell containing reactor was scaled up to 5 l, the hydrogen production rate was 0.500–0.588 l H2/(l POME h) for HRT 2–10 h, but after a thermal treatment (60 8C, 1 h) the rate increase to 0.632 l H2/(l POME h) at HRT 2 h. The main soluble metabolites were butyric acid and acetic acid, followed by propionic acid and ethanol.
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Quorum quenching bacteria can be used to inhibit the biofouling of reverse osmosis membranes
Oh, Hyun-Suk,Tan, Chuan Hao,Low, Jiun Hui,Rzechowicz, Miles,Siddiqui, Muhammad Faisal,Winters, Harvey,Kjelleberg, Staffan,Fane, Anthony G.,Rice, Scott A. Pergamon Press 2017 Water research Vol.112 No.-
<P><B>Abstract</B></P> <P>Over the last few decades, significant efforts have concentrated on mitigating biofouling in reverse osmosis (RO) systems, with a focus on non-toxic and sustainable strategies. Here, we explored the potential of applying quorum quenching (QQ) bacteria to control biofouling in a laboratory-scale RO system. For these experiments, <I>Pantoea stewartii</I> was used as a model biofilm forming organism because it was previously shown to be a relevant wastewater isolate that also forms biofilms in a quorum sensing (QS) dependent fashion. A recombinant <I>Escherichia coli</I> strain, which can produce a QQ enzyme, was first tested in batch biofilm assays and significantly reduced biofilm formation by <I>P. stewartii</I>. Subsequently, RO membranes were fouled with <I>P. stewartii</I> and the QQ bacterium was introduced into the RO system using two different strategies, direct injection and immobilization within a cartridge microfilter. When the QQ bacterial cells were directly injected into the system, <I>N-</I>acylhomoserine lactone signals were degraded, resulting in the reduction of biofouling. Similarly, the QQ bacteria controlled biofouling when immobilized within a microfilter placed downstream of the RO module to remove QS signals circulating in the system. These results demonstrate the proof-of-principle that QQ can be applied to control biofouling of RO membranes and may be applicable for use in full-scale plants.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A recombinant Quorum Quenching (QQ) bacterium controlled biofilm formation. </LI> <LI> Direct injection of QQ bacteria in a lab-scale RO system mitigated biofouling. </LI> <LI> QQ bacteria controlled biofouling when immobilized within a microfilter cartridge. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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Eun-Kyung Jang,정병권,Gun-Seok Park,Abdur Rahim Khan,Sung-Jun Hong,박영준,WON-CHAN KIM,JAE-HO SHIN,Khalid M S Al-Ghamdi,Bassam Oudh Al-Johny,Yasir Anwar,Muhammad Faisal Siddiqui,Ihsan Ullah 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.1
Photorhabdus spp. has a high molecular weight Tc toxin with insecticidal activity. These toxins have been suggested as an alternative to BT toxin from Bacillus thuringiensis. Herein, we constructed a cosmid library with the genome of M1021 and screened the Escherichia coli clones showing insect toxicity by injecting each clone into Galleria mellonella larvae. In a total of 1020 clones, one clone with high insecticidal activity was selected and the nucleotide sequence of the cosmid of the clone was determined. In cosmid PtC28, a gene with 87% homology to the tccB gene of Photorhabdus temperata was found. Consequently, we have isolated the tccB gene cassette from the M1021 and expressed in E. coli expression vectors. The toxin was produced in the form of inclusion bodies but the denatured and refolded recombinant TccB showed strong mortality to the G. mellonella larvae.
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