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        Biohydrogen production from palm oil mill effluent using immobilized mixed culture

        Lakhveer Singh,Zularisam A. Wahid,Muhammad Faisal Siddiqui,Anwer Ahmad,Mohd Hasbi Ab. Rahim,Mimi Sakinah 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.2

        Cell immobilization techniques were adopted to bio-hydrogen production using immobilized anaerobic sludge as the seed culture. Palm oil mill effluent (POME) was used as the substrate carbon source. It was found that with a POME concentration of 20 g COD/l in the feed, the suspended-cell containing reactor was able to produce hydrogen at an optimal rate of 0.348 l H2/(l POME h) at HRT 6 h. However, the immobilized-cell containing reactor exhibited a better hydrogen production rate of 0.589 l H2/(l POME h), which occurred at HRT 2 h. When the immobilized-cell containing reactor was scaled up to 5 l, the hydrogen production rate was 0.500–0.588 l H2/(l POME h) for HRT 2–10 h, but after a thermal treatment (60 8C, 1 h) the rate increase to 0.632 l H2/(l POME h) at HRT 2 h. The main soluble metabolites were butyric acid and acetic acid, followed by propionic acid and ethanol.

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        Methods for enhancing bio-hydrogen production from biological process: A review

        Lakhveer Singh,Zularisam A. Wahid 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.21 No.1

        Hydrogen (H2) is considered a clean source of energy and an ideal substitute for fossil fuel owing to itshigh energy content (122 kJ/g), recyclability and non-polluting nature. The conventional physicochemicalmethods for hydrogen production are costly owing to high energy input requirements. Hydrogenproduction by means of biological processes is considered the most environmental friendly andrelatively easy to operate, with successful operation under ambient conditions and promisingtechniques, and having significant advantages compared with conventional chemical processes. Themajor bottlenecks in biological processes are the low hydrogen yield and production rates at a largescale. The target is improvement of the biological method for hydrogen production and devising patheven better in comparison to the conventional methods. The present review article aims to highlightvarious techniques such as pretreatment, cell immobilization, sequential fermentation, combinedfermentation that have been used in biological processes for enhancing hydrogen production. Futuredevelopments on these techniques are also outlined.

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