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Biohydrogen production from palm oil mill effluent using immobilized mixed culture
Lakhveer Singh,Zularisam A. Wahid,Muhammad Faisal Siddiqui,Anwer Ahmad,Mohd Hasbi Ab. Rahim,Mimi Sakinah 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.2
Cell immobilization techniques were adopted to bio-hydrogen production using immobilized anaerobic sludge as the seed culture. Palm oil mill effluent (POME) was used as the substrate carbon source. It was found that with a POME concentration of 20 g COD/l in the feed, the suspended-cell containing reactor was able to produce hydrogen at an optimal rate of 0.348 l H2/(l POME h) at HRT 6 h. However, the immobilized-cell containing reactor exhibited a better hydrogen production rate of 0.589 l H2/(l POME h), which occurred at HRT 2 h. When the immobilized-cell containing reactor was scaled up to 5 l, the hydrogen production rate was 0.500–0.588 l H2/(l POME h) for HRT 2–10 h, but after a thermal treatment (60 8C, 1 h) the rate increase to 0.632 l H2/(l POME h) at HRT 2 h. The main soluble metabolites were butyric acid and acetic acid, followed by propionic acid and ethanol.