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- 저자 : Mingzhou Li (검색결과 11 건)
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Pengxiang Li,Mingzhou Bai,Zijun Wei,Xin Li,Hai Shi 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.8
With the increase in the use of high-speed trains, karst collapse disasters have become increasingly prominent during the construction and operation of railway projects. To study the propagation characteristics of vibration wave caused by single and double railways in karst area, as well as the influence of sinkholes on the stability of the roadbed, a coupling model of vehicle-track-subgrade (including underlying sinkhole) considering track irregularity was established based on the geological engineering characteristics of a karst site. The results show that the subgrade stability is controlled by multiple factors related to the upper load, the stability of the caves, and the soil state; increasing the upper load of the track further develops the sinkhole and has a negative effect on the subgrade stability. The amplitude of vertical dynamic displacement on the subgrade surface can reach 1.31 mm under the superimposed effect of the vibration of double railways, which is approximately 50% higher than that of a single railway. Under the long-term cumulative effect of train vibrations, the sinkholes grow faster in dynamic displacement amplitude than the roadbed surface, and will lead to abrupt displacement of subgrade surface.
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Yixin Wang,Long Jin,Jideng Ma,Li Chen,Yuhua Fu,Keren Long,Silu Hu,Yang Song,Dazhi Shang,Qianzi Tang,Xun Wang,Xuewei Li,Mingzhou Li 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.2
Objective: Hemicastration is a unilateral orchiectomy to remove an injured testis, which can induce hormonal changes and compensatory hypertrophy of the remaining testis, and may influence spermatogenesis. However, the underlying molecular mechanisms are poorly understood. Here, we investigated the impact of hemicastration on remaining testicular function. Methods: Prepubertal mice (age 24 days) were hemicastrated, and their growth was monitored until they reached physical maturity (age 72 days). Subsequently, we determined testis DNA methylation patterns using reduced representation bisulfite sequencing of normal and hemicastrated mice. Moreover, we profiled the testicular gene expression patterns by RNA sequencing (RNA-seq) to examine whether methylation changes affected gene expression in hemicastrated mice. Results: Hemicastration did not significantly affect growth or testosterone (p>0.05) compared with control. The genome-wide DNA methylation pattern of remaining testis suggested that substantial genes harbored differentially methylated regions (1,139) in gene bodies, which were enriched in process of protein binding and cell adhesion. Moreover, RNA-seq results indicated that 46 differentially expressed genes (DEGs) involved in meiotic cell cycle, synaptonemal complex assembly and spermatogenesis were upregulated in the hemicastration group, while 197 DEGs were downregulated, which were related to arachidonic acid metabolism. Integrative analysis revealed that proteasome 26S subunit ATPase 3 interacting protein gene, which encodes a protein crucial for homologous recombination in spermatocytes, exhibited promoter hypomethylation and higher expression level in hemicastrated mice. Conclusion: Global profiling of DNA methylation and gene expression demonstrated that hemicastration-induced compensatory response maintained normal growth and testicular morphological structure in mice.
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Estimation of Growth Curves and Suitable Slaughter Weight of the Liangshan Pig
Luo, Jia,Lei, Huaigang,Shen, Linyuan,Yang, Runlin,Pu, Qiang,Zhu, Kangping,Li, Mingzhou,Tang, Guoqing,Li, Xuewei,Zhang, Shunhua,Zhu, Li Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.9
The Liangshan pig is a traditional Chinese small-sized breed; it has a relatively long feeding period and low meat production ability but superior meat quality. This study utilized three non-linear growth models (Von Bertalanffy, Gompertz, and logistic) to fit the growth curve of Liangshan pigs from an unselected, random-bred pig population and estimate the pigs most suitable slaughter weight. The growth development data at 20 time points of 275 Liangshan pigs (from birth to 250 d) were collected. To analyze the relative gene expression related to development, seven slaughter weight phases (50, 58, 66, 74, 82, 90, and 98 kg) (20 pigs per phase) were examined. We found that the Liangshan pig growth curve fit the typical S-curve well and that their growth turning point was 193.4 days at a weight of 62.5 kg, according to the best fit Von Bertalanffy model based on the goodness of fit criteria. Furthermore, we estimated that the most suitable slaughter weight was 62.5 to 74.9 kg based on the growth curve and the relative expression levels of growth-related genes.
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Estimation of Growth Curves and Suitable Slaughter Weight of the Liangshan Pig
Jia Luo,Huaigang Lei,Linyuan Shen,Runlin Yang,Qiang Pu,Kangping Zhu,Mingzhou Li,Guoqing Tang,Xuewei Li,Shunhua Zhang,Li Zhu 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.9
The Liangshan pig is a traditional Chinese small-sized breed; it has a relatively long feeding period and low meat production ability but superior meat quality. This study utilized three non-linear growth models (Von Bertalanffy, Gompertz, and logistic) to fit the growth curve of Liangshan pigs from an unselected, random-bred pig population and estimate the pigs most suitable slaughter weight. The growth development data at 20 time points of 275 Liangshan pigs (from birth to 250 d) were collected. To analyze the relative gene expression related to development, seven slaughter weight phases (50, 58, 66, 74, 82, 90, and 98 kg) (20 pigs per phase) were examined. We found that the Liangshan pig growth curve fit the typical S-curve well and that their growth turning point was 193.4 days at a weight of 62.5 kg, according to the best fit Von Bertalanffy model based on the goodness of fit criteria. Furthermore, we estimated that the most suitable slaughter weight was 62.5 to 74.9 kg based on the growth curve and the relative expression levels of growth-related genes.
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Coat colour phenotype of Qingyu pig is associated with polymorphisms of melanocortin receptor 1 gene
Xiaoqian Wu,Zhendong Tan,Linyuan Shen,Qiong Yang,Xiao Cheng,Kun Liao,Lin Bai,Surong Shuai,Mingzhou Li,Xuewei Li,Shunhua Zhang,Li Zhu 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.7
Objective: Qingyu pig, a Chinese indigenous pig breed, exhibits two types of coat colour phenotypes, including pure black and white with black spotting respectively. Melanocortin receptor 1 (MC1R) and agouti signaling protein (ASIP) are two widely reported pivotal genes that significantly affect the regulation of coat colour. The objectives of this study were to investigate whether the polymorphisms of these two genes are associated with coat colour and analyze the molecular mechanism of the coat colour separation in Qingyu pig. Methods: We studied the phenotype segregation and used polymerase chain reaction amplification and Sanger sequencing to investigate the polymorphism of MC1R and ASIP in 121 Qingyu pigs, consisting of 115 black and 6 white with black spotted pigs. Results: Coat colour of Qingyu pig is associated with the polymorphisms of MC1R but not ASIP. We only found 2 haplotypes, EQY and Eqy, based on the 13 observed mutations from MC1R gene. Among which, Eqy presented a recessive inheritance mode in black spotted Qingyu pigs. Further analysis revealed a g.462-463CC insertion that caused a frameshift mutation and a premature stop codon, thus changed the first transmembrane domain completely and lost the remaining six transmembrane domains. Altogether, our results strongly support that the variety of Qingyu pig’s coat colour is related to MC1R. Conclusion: Our findings indicated that black coat colour in Qingyu pig was dominant to white with black spotted phenotype and MC1R gene polymorphism was associated with coat colour separation in Qingyu pig.
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Luo, Jia,Shen, Linyuan,Gan, Mailin,Jiang, Anan,Chen, Lei,Ma, Jideng,Jin, Long,Liu, Yihui,Tang, Guoqing,Jiang, Yanzhi,Li, Mingzhou,Li, Xuewei,Zhang, Shunhua,Zhu, Li Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.8
Objective: Investigation of muscle growth at different developmental stages is an appropriate strategy for studying the mechanisms underlying muscle development and differences in phenotypes. In particular, the muscle development mechanisms and the difference between the fastest and slowest growth rates. Methods: In this study, we used a growth curve model to fit the growth inflection point (IP) of QingYu pigs and compared differences in the long non-coding RNA (lncRNA) transcriptome of muscle both at the growth IP and plateau phase (PP). Results: The growth curve of the QingYu pig had a good fit (R<sup>2</sup> = 0.974) relative to a typical S-curve and reached the IP at day 177.96. At the PP, marbling, intramuscular fat, and monounsaturated fatty acids had increased significantly and the percentage of lean muscle and polyunsaturated fatty acids had decreased. A total of 1,199 mRNAs and 62 lncRNAs were differentially expressed at the IP compared with the PP. Additional to gene ontology and Kyoto encyclopedia of genes and genomes pathway analyses, these differentially expressed protein coding genes were principally related to muscle growth and lipid metabolism. Conclusion: Our results suggest that the identified differentially expressed lncRNAs, could play roles in muscle growth, fat deposition and regulation of fatty acid composition at the IP and PP.
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Tengfei Li,Mingzhou Su,Jiangran Guo 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.38 No.4
Based on the spatial substructure hybrid simulation test (SHST) method, the seismic performance of a high-strength steel composite K-eccentrically braced frame (K-HSS-EBF) structure system is studied. First, on the basis of the existing pseudostatic experiments, a numerical model corresponding to the experimental model was established using OpenSees, which mainly simulated the shear effect of the shear links. A three-story and five-span spatial K-HSS-EBF was taken as the prototype, and SHST was performed with a half-scale SHST model. According to the test results, the validity of the SHST model was verified, and the main seismic performance indexes of the experimental substructure under different seismic waves were studied. The results show that the hybrid simulation results are basically consistent with the numerical simulation results of the global structure. The deformation of each story is mainly concentrated in the web of the shear link owing to shear deformation. The maximum interstory drifts of the model structure during Strength Level Earthquake (SLE) and Maximum Considered Earthquake (MCE) meet the demands of interstory limitations in the Chinese seismic design code of buildings. In conclusion, the seismic response characteristics of the K-HSS-EBFs are successfully simulated using the spatial SHST, which shows that the K-HSS-EBFs have good seismic performance.
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Tengfei Li,Mingzhou Su,Yan Sui 국제구조공학회 2020 Steel and Composite Structures, An International J Vol.34 No.5
High-strength steel composite Y-eccentrically braced frame (Y-HSS-EBF) is a novel structural system. In this study, the spatial substructure hybrid simulation test (SHST) method is used to further study the seismic performance of Y-HSS-EBF. Firstly, based on the cyclic loading tests of two single-story single-span Y-HSS-EBF planar specimens, a finite element model in OpenSees was verified to provide a reference for the numerical substructure analysis model for the later SHST. Then, the SHST was carried out on the OpenFresco test platform. A three-story spatial Y-HSS-EBF model was taken as the prototype, the top story was taken as the experimental substructure, and the remaining two stories were taken as the numerical substructure to be simulated in OpenSees. According to the test results, the validity of the SHST was verified, and the main seismic performance indexes of the SHST model were analyzed. The results show that, the SHST based on the OpenFresco platform has good stability and accuracy, and the results of the SHST agree well with the global numerical model of the structure. Under strong seismic action, the plastic deformation of Y-HSS-EBF mainly occurs in the shear link, and the beam, beam-columns and braces can basically remain in the elastic state, which is conducive to post-earthquake repair.
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Jideng Ma,Mingzhou Li,Huiyu Wang,Xuewei Li 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.5
During the last decade, DNA mutations in the porcine ryanodine receptor 1 gene (RYR1, C1843T) and the estrogen receptor 1 gene (ESR1, T1665G), have been widely used in marker-assisted selection (MAS) for the pig industry. These 2 well-characterized SNPs in RYR1 and ESR1 are responsible for porcine stress syndrome (PSS)and litter size, respectively. Here, we describe a reliable,high-efficiency method for the genotyping of these 2 genes using the high-resolution melting (HRM) method. The HRM approach exhibited high-accuracy and repeatability,comparable with the classic PCR-restriction fragment length polymorphism (PCR-RFLP) approach, and is potentially suitable for large-scale genotyping in commercial pig farms.
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Chi Xiangyu,Li Shengjie,Gu Mingzhou,Li Yaru,Zhu Xixi,Wang Naihua 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.8
The fluid retention effect of the Volume Control Tank (VCT) leads to a long time delay in Reactor Coolant System (RCS) concentration during the boration process. A coupled model combining a lumpedparameter sub-model and a computational fluid dynamics sub-model is currently used to investigate the concentration dynamic characteristic of RCS during the boration process. This model is validated by comparison with experimental data, and the predicted results show excellent agreement with experimental data. We provide detailed fields in VCT and concentration variations of RCS to study the interaction between mixing in VCT and the transient responses of RCS. Moreover, the impacts of the inlet flow rate, inlet nozzle diameter, original concentration, and replenishing temperature of VCT on the RCS concentration characteristic are studied. The inlet flow rate and nozzle diameter of VCT remarkably affect the RCS concentration characteristic. Too-large or too-small inlet flow rates and nozzle diameters will lead to unacceptable long delays. In this work, the optimal inlet flow rate and nozzle diameter of VCT are 5 m3 /h and 58.8 mm, respectively. Besides, the impacts of the original concentration and replenishing temperature of VCT are negligible under normal operating conditions.
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